FastQCFastQC Report
Mon 6 Jun 2016
ERR1211561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4354612
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98640.22651845904985338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84360.19372564076891352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55950.12848446658393448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.046.1474531
GTATCAA95800.036.80651
TCAACGC111100.030.7894384
ATCAACG112950.030.3669473
CAACGCA112900.030.2961145
AACGCAG116050.029.5513526
GTACCTG37100.028.7159331
TATCAAC122950.028.3856622
GTACATG128850.028.2436811
TACATGG129850.026.9135192
TACCTGG37850.026.7175252
ACGCAGA134100.025.3638067
ACATGGG133850.025.274273
CGCAGAG139150.024.3425838
CATGGGG79950.023.5085564
ACCTGGG48100.020.80653
TATACCG3202.0008883E-1120.555485
GCAGAGT160200.020.5281669
GAGTACT97000.019.13307812-13
AGAGTAC142000.019.12379510-11
Produced by FastQC (version 0.11.5)