FastQCFastQC Report
Mon 6 Jun 2016
ERR1211561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4354612
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118510.2721482419099566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104410.23976877848129755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71240.16359666486933852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65400.046.924151
GTATCAA111750.037.3748441
TCAACGC136200.030.152894
ATCAACG136650.029.9507793
CAACGCA137550.029.8904255
AACGCAG144300.028.68766
GTACCTG37400.028.4859051
TATCAAC146400.028.3280352
TACCTGG38400.026.5715872
GTACATG137000.025.3594671
ACGCAGA164850.024.940137
TACATGG139200.024.4223822
CGCAGAG168500.024.3720028
ACATGGG139400.023.4609763
ACCTGGG46450.021.6484933
GCAGAGT189800.021.3643289
CATGGGG86450.020.8174924
AGAGTAC173150.020.04022810-11
GAGTACT118050.019.98054912-13
CAGAGTA186700.018.6109510-11
Produced by FastQC (version 0.11.5)