##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211561_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4354612 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96121858847585 33.0 31.0 34.0 30.0 34.0 2 32.23574913218445 34.0 31.0 34.0 30.0 34.0 3 32.330928909395375 34.0 31.0 34.0 30.0 34.0 4 35.97910904576573 37.0 35.0 37.0 35.0 37.0 5 35.8705397863231 37.0 35.0 37.0 35.0 37.0 6 35.89743908297685 37.0 35.0 37.0 35.0 37.0 7 35.80208868206857 37.0 35.0 37.0 35.0 37.0 8 35.78208437399245 37.0 35.0 37.0 33.0 37.0 9 37.40911199436368 39.0 37.0 39.0 34.0 39.0 10-11 37.36679444689906 39.0 37.0 39.0 34.0 39.0 12-13 37.27311480333954 39.0 37.0 39.0 34.0 39.0 14-15 38.481198784185594 40.0 38.0 41.0 34.0 41.0 16-17 38.40575899758693 40.0 38.0 41.0 33.5 41.0 18-19 38.39081690860173 40.0 38.0 41.0 33.5 41.0 20-21 38.31898881002486 40.0 38.0 41.0 34.0 41.0 22-23 38.210456637698144 40.0 38.0 41.0 33.5 41.0 24-25 38.09830175455356 40.0 37.0 41.0 33.0 41.0 26-27 37.89078039558978 40.0 37.0 41.0 33.0 41.0 28-29 37.91480446478354 40.0 37.0 41.0 33.0 41.0 30-31 37.80786967931931 40.0 37.0 41.0 33.0 41.0 32-33 37.634166488311706 40.0 37.0 41.0 32.0 41.0 34-35 37.4949209022526 40.0 37.0 41.0 32.0 41.0 36-37 37.40379418418908 40.0 36.5 41.0 31.5 41.0 38-39 37.32749037112836 40.0 36.0 41.0 31.0 41.0 40-41 37.1766544298321 39.5 36.0 41.0 31.0 41.0 42-43 36.9857216900151 39.0 36.0 41.0 30.5 41.0 44-45 36.78868392867149 39.0 35.5 41.0 30.0 41.0 46-47 36.548024714945896 39.0 35.0 40.5 30.0 41.0 48-49 36.30508504546444 39.0 35.0 40.0 29.0 41.0 50-51 36.100013617745965 39.0 35.0 40.0 28.0 41.0 52-53 35.886781646677136 38.5 35.0 40.0 28.0 41.0 54-55 35.6622014774221 38.0 34.0 40.0 27.0 41.0 56-57 35.445262746715436 38.0 34.0 40.0 26.5 41.0 58-59 35.16948972721335 38.0 34.0 40.0 26.0 41.0 60-61 34.85218809391055 37.5 33.5 40.0 26.0 41.0 62-63 34.50439373243816 37.0 33.0 40.0 25.5 41.0 64-65 34.173434165891244 36.5 32.5 39.5 25.0 41.0 66-67 33.803255835422306 36.0 32.0 39.0 23.5 40.0 68-69 33.40168274463947 35.5 31.5 39.0 23.0 40.0 70-71 33.02447003315106 35.0 31.0 38.0 22.5 40.0 72-73 32.595302865100265 35.0 31.0 37.5 22.0 40.0 74-75 32.195335887560134 35.0 30.5 37.0 21.5 39.0 76-77 30.721254844289227 33.5 28.5 35.5 20.0 38.0 78-79 31.600528703820224 34.0 30.0 36.0 21.0 38.5 80-81 31.560176429036616 34.0 30.0 36.0 20.5 37.5 82-83 31.269802912406433 34.0 30.0 35.0 20.0 37.0 84-85 30.93751073574408 34.0 30.0 35.0 20.0 36.5 86-87 30.637416720479344 34.0 29.0 35.0 19.5 36.0 88-89 30.34083645110058 34.0 29.0 35.0 18.5 36.0 90-91 30.05432918018873 34.0 29.0 35.0 18.0 35.5 92-93 29.813911893872522 34.0 29.0 35.0 18.0 35.0 94-95 29.586720470158994 33.5 29.0 35.0 17.5 35.0 96-97 29.340597279390217 33.0 27.5 35.0 16.5 35.0 98-99 29.128689536518984 33.0 27.0 35.0 15.0 35.0 100 27.319319608727483 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23784320939122594 1101 2 0.15273075320286011 1101 3 0.13331214008418613 1101 4 0.13065618926324163 1101 5 0.09244831282870791 1101 6 0.08584260515709019 1101 7 0.18519079878662126 1101 8 0.07903303891579583 1101 9 0.05550321040092854 1101 10-11 0.08914831228393183 1101 12-13 0.042926229953074824 1101 14-15 0.07986230733011013 1101 16-17 0.09020311376887946 1101 18-19 0.09176973617726958 1101 20-21 0.025596737736833575 1101 22-23 0.04589169422161632 1101 24-25 -0.04926035530854023 1101 26-27 -0.08003383539142561 1101 28-29 0.05067645450079539 1101 30-31 0.055282528811439136 1101 32-33 0.03932125714947432 1101 34-35 -0.08175561169574053 1101 36-37 -0.06194582807123794 1101 38-39 -0.1549861167709068 1101 40-41 -0.11123319187893799 1101 42-43 -0.08687044425197143 1101 44-45 -0.13656494791286633 1101 46-47 -0.23216115221580225 1101 48-49 -0.22282461497702855 1101 50-51 -0.3620695805149836 1101 52-53 -0.313134817003494 1101 54-55 -0.27643342253884384 1101 56-57 -0.42039569334995264 1101 58-59 -0.4267794528674287 1101 60-61 -0.5742250592117344 1101 62-63 -0.6457352083260375 1101 64-65 -0.581266476585732 1101 66-67 -0.5313916790149946 1101 68-69 -0.6281906382897517 1101 70-71 -0.5241740768102616 1101 72-73 -0.7563175761171834 1101 74-75 -0.7611718264840235 1101 76-77 -0.6376750552606296 1101 78-79 -0.5997499027124888 1101 80-81 -0.6323024019788441 1101 82-83 -0.7635221378233013 1101 84-85 -0.7910956173092103 1101 86-87 -0.7958861365985079 1101 88-89 -0.7548290692062345 1101 90-91 -0.9290013708444995 1101 92-93 -1.0181562594629199 1101 94-95 -1.035535917439443 1101 96-97 -1.127303193614818 1101 98-99 -1.3004654243699498 1101 100 -1.1668829931250997 1102 1 0.2493289032948134 1102 2 0.15067796350486873 1102 3 0.12492416987920762 1102 4 0.09952573614798865 1102 5 0.10495657117806445 1102 6 0.15981627159454348 1102 7 0.1421839506385112 1102 8 0.16020786342203763 1102 9 0.16390300889107579 1102 10-11 0.18195254742288824 1102 12-13 0.18332981349447408 1102 14-15 0.17221210343007698 1102 16-17 0.23341162498614665 1102 18-19 0.13347899692783471 1102 20-21 0.20111454696682074 1102 22-23 0.15533841852207075 1102 24-25 0.18594141807623288 1102 26-27 0.1675234398619594 1102 28-29 0.25067161287111617 1102 30-31 0.27771649572411405 1102 32-33 0.1937329317282135 1102 34-35 0.17932559162697004 1102 36-37 0.14693418041080264 1102 38-39 0.1599133244192288 1102 40-41 0.14695743595171962 1102 42-43 0.08676372588656989 1102 44-45 0.056460715175894904 1102 46-47 -0.019379954654027642 1102 48-49 -0.1185466367925585 1102 50-51 -0.1437209684692533 1102 52-53 -0.011561195427717053 1102 54-55 -0.05575837678851059 1102 56-57 0.006375117384003204 1102 58-59 0.014955670765736784 1102 60-61 -0.013890692588823583 1102 62-63 -0.1183400112226849 1102 64-65 -0.16072681260709487 1102 66-67 -0.30705001807860555 1102 68-69 -0.25514491001671047 1102 70-71 -0.3200653718052351 1102 72-73 -0.32740878751744873 1102 74-75 -0.43499322673235596 1102 76-77 -0.23467710590529833 1102 78-79 -0.07008388935939536 1102 80-81 -0.16946482088154724 1102 82-83 -0.31768419268448156 1102 84-85 -0.24985320610408834 1102 86-87 -0.16002295982754333 1102 88-89 -0.3877028460818792 1102 90-91 -0.399570237817354 1102 92-93 -0.5308100731349477 1102 94-95 -0.4005483708464155 1102 96-97 -0.41956954821086967 1102 98-99 -0.6353675376493761 1102 100 -0.5559661600657151 1103 1 0.1938777591519134 1103 2 0.11985668953735029 1103 3 0.08782018291722693 1103 4 0.08535869190406231 1103 5 0.08521402859562954 1103 6 0.06609275849989871 1103 7 0.08301675556681687 1103 8 0.06763404389310779 1103 9 0.15499700993742493 1103 10-11 0.08943156839728772 1103 12-13 0.10086340974549302 1103 14-15 0.10347120451485381 1103 16-17 0.20368743260790723 1103 18-19 0.10928139812624948 1103 20-21 0.08499585231666629 1103 22-23 -0.03069227396428431 1103 24-25 -0.11035026536178094 1103 26-27 -0.021477721765791102 1103 28-29 0.03893545015100841 1103 30-31 0.2011476355341415 1103 32-33 0.1547743725975934 1103 34-35 0.16045307643830853 1103 36-37 0.13730377649809355 1103 38-39 0.09210326645159483 1103 40-41 0.12560619077960666 1103 42-43 0.16864212985858984 1103 44-45 0.1575291851942282 1103 46-47 0.18977989708583465 1103 48-49 0.16581517942567814 1103 50-51 0.1986688868853932 1103 52-53 -0.001125183322088219 1103 54-55 0.0496104621129021 1103 56-57 -0.037316767584279376 1103 58-59 -0.004874803898907487 1103 60-61 0.017737357598129222 1103 62-63 0.049737682243893744 1103 64-65 -0.030905846693862316 1103 66-67 -0.004544408250445997 1103 68-69 -0.1208551508769844 1103 70-71 -0.13587465115625008 1103 72-73 -0.22355079089822283 1103 74-75 -0.04986027941654925 1103 76-77 -0.015174963490771631 1103 78-79 -0.0056118226116552705 1103 80-81 -0.01806669823530882 1103 82-83 -0.04561372249693463 1103 84-85 -0.10862889900533901 1103 86-87 -0.1589037365184467 1103 88-89 -0.15208320751396798 1103 90-91 -0.1904074152274795 1103 92-93 -0.33075488642185036 1103 94-95 -0.21866945395840887 1103 96-97 -0.24922905731269296 1103 98-99 -0.1319675902410431 1103 100 -0.35019640368455995 1104 1 0.2735633346733586 1104 2 0.2100490925996965 1104 3 0.16541094845231896 1104 4 0.09900349939699993 1104 5 0.1081810086626831 1104 6 0.09874667481024346 1104 7 0.16918713548385966 1104 8 0.05909873441408564 1104 9 0.06819543550957263 1104 10-11 0.12556747920009315 1104 12-13 0.09855313960336787 1104 14-15 0.10165981559874382 1104 16-17 0.14891411809246335 1104 18-19 0.16985163831245131 1104 20-21 0.11882156815535438 1104 22-23 0.040895202282598575 1104 24-25 0.010048959903848242 1104 26-27 0.09057080613614943 1104 28-29 0.10784323155041164 1104 30-31 0.13836525755868934 1104 32-33 0.006957454088784232 1104 34-35 0.10238618856455162 1104 36-37 0.15260801310432015 1104 38-39 0.10112262203077194 1104 40-41 0.08250375999912762 1104 42-43 -0.036866588409090184 1104 44-45 0.06632814392877862 1104 46-47 0.1471504361797713 1104 48-49 0.008823336990758435 1104 50-51 -0.038135481658223114 1104 52-53 -0.02556582765771509 1104 54-55 0.04783192598399211 1104 56-57 0.03490120151204934 1104 58-59 -0.031053062470640214 1104 60-61 -0.061475172870892436 1104 62-63 -0.012295095866477368 1104 64-65 0.015872437588747346 1104 66-67 -0.014885017227349806 1104 68-69 -0.17975666493690312 1104 70-71 0.020248509457296393 1104 72-73 0.012419678179938387 1104 74-75 -0.09165543990779668 1104 76-77 -0.025658686325513713 1104 78-79 0.1506935444211308 1104 80-81 0.09819209853073474 1104 82-83 0.032697649253524474 1104 84-85 -0.1025393053700121 1104 86-87 -0.1841329730432264 1104 88-89 -0.11283836052751539 1104 90-91 -0.08864180971502478 1104 92-93 -0.1953543167229448 1104 94-95 -0.13474516722850538 1104 96-97 -0.4068235015580548 1104 98-99 -0.40461665506427735 1104 100 -0.240733422711358 1105 1 0.2591108777354343 1105 2 0.13375273742296656 1105 3 0.08387693721135037 1105 4 0.04243305177257639 1105 5 0.09790436863045215 1105 6 0.04534945108492394 1105 7 0.11084335178761506 1105 8 0.07701590631877053 1105 9 0.1560951989199424 1105 10-11 0.13632729628746887 1105 12-13 0.10441241123117351 1105 14-15 0.10393143649447012 1105 16-17 0.1083190250562609 1105 18-19 0.1323742012219924 1105 20-21 0.09799654574055694 1105 22-23 0.13079368400121183 1105 24-25 0.07408765652800042 1105 26-27 0.1334514182278923 1105 28-29 0.10987944773567904 1105 30-31 0.1742804229936965 1105 32-33 0.20106458495563828 1105 34-35 0.19843824717084146 1105 36-37 0.16395130604494312 1105 38-39 0.06182008254321403 1105 40-41 0.11793468688543385 1105 42-43 0.15396761222208255 1105 44-45 0.19723797227292295 1105 46-47 0.20746941252475892 1105 48-49 0.16167075642586326 1105 50-51 0.005657126199388074 1105 52-53 -0.003953843543584412 1105 54-55 0.06814353112023497 1105 56-57 0.19498156398000788 1105 58-59 0.06214086302139776 1105 60-61 0.0841044964170905 1105 62-63 0.09699119536390555 1105 64-65 0.18337182537850794 1105 66-67 0.08320710856407487 1105 68-69 0.06854847545673692 1105 70-71 0.08826444963447955 1105 72-73 0.07045659149492423 1105 74-75 -0.021961920251904132 1105 76-77 -0.026390647847115645 1105 78-79 0.08936713663381823 1105 80-81 -0.07265477090259864 1105 82-83 -0.045900196753873246 1105 84-85 -0.04662187376969129 1105 86-87 -0.011536095710411587 1105 88-89 -0.09293715842309425 1105 90-91 -0.10731185209577276 1105 92-93 -0.14187901150651783 1105 94-95 -0.12529175247778923 1105 96-97 0.05253766199687249 1105 98-99 0.06821318307523683 1105 100 0.12233988255246331 1106 1 0.22171735338175225 1106 2 0.1625542822500563 1106 3 0.15403625117683362 1106 4 0.1362512269323588 1106 5 0.09802480733283403 1106 6 0.1430592650962339 1106 7 0.1159897562908867 1106 8 0.13418096139157143 1106 9 0.06913708495476811 1106 10-11 0.1496755519433961 1106 12-13 0.15058569667364452 1106 14-15 0.17396637057239417 1106 16-17 0.15858933160898658 1106 18-19 0.10753220113268469 1106 20-21 0.13338398235846682 1106 22-23 0.15090631083393902 1106 24-25 0.24967565687737192 1106 26-27 0.15944191884555892 1106 28-29 0.2354783365270805 1106 30-31 0.31538890360169347 1106 32-33 0.33733593393960604 1106 34-35 0.3046558070553118 1106 36-37 0.2637440976221441 1106 38-39 0.2834852376878416 1106 40-41 0.3065406666767956 1106 42-43 0.23413295889363184 1106 44-45 0.3064855936575128 1106 46-47 0.24330871459129355 1106 48-49 0.08346378711794955 1106 50-51 0.1308820675075566 1106 52-53 0.13779014705475134 1106 54-55 0.12703320966052445 1106 56-57 0.17581616795796862 1106 58-59 0.21840285793280856 1106 60-61 0.21451731035084975 1106 62-63 0.28324274540020866 1106 64-65 0.18312766773266986 1106 66-67 0.21631699056940334 1106 68-69 0.12446138417477215 1106 70-71 0.23579654801742578 1106 72-73 0.1221613915353359 1106 74-75 0.13340390790028067 1106 76-77 0.18109539595105417 1106 78-79 0.20349747030581966 1106 80-81 0.2024635282992584 1106 82-83 0.18060003374425904 1106 84-85 0.1452562933765087 1106 86-87 0.05472457829198518 1106 88-89 0.1405531679011638 1106 90-91 0.11196570369182623 1106 92-93 0.1401276470891233 1106 94-95 0.18411909272381521 1106 96-97 0.2516636442087652 1106 98-99 0.10181492009507664 1106 100 0.046170775255873764 1107 1 0.2126158168715797 1107 2 0.1629714731404519 1107 3 0.12040890216021438 1107 4 0.060579593680088806 1107 5 0.006965313046578103 1107 6 0.08519224851372798 1107 7 0.04578741080316462 1107 8 0.07505072893282261 1107 9 0.05741898966045511 1107 10-11 0.07206831970189143 1107 12-13 0.10191374555709132 1107 14-15 0.12352467624553043 1107 16-17 0.09181991086275332 1107 18-19 0.05471042966168227 1107 20-21 0.062165731294356874 1107 22-23 0.14818882031548242 1107 24-25 0.1145161339478733 1107 26-27 0.1594790525242118 1107 28-29 0.20949079868546505 1107 30-31 0.1680117056353012 1107 32-33 0.1394296696940458 1107 34-35 0.13026905658184518 1107 36-37 0.19996566292696372 1107 38-39 0.21104076068288435 1107 40-41 0.19684437800350452 1107 42-43 0.28617166493202006 1107 44-45 0.3079770978072389 1107 46-47 0.3156848416703113 1107 48-49 0.21077811369366373 1107 50-51 0.19904951136215487 1107 52-53 0.24439001398681626 1107 54-55 0.34724258419425524 1107 56-57 0.2311911313872841 1107 58-59 0.11220166296624967 1107 60-61 0.2016982613774232 1107 62-63 0.30213063532659845 1107 64-65 0.3115630720456011 1107 66-67 0.2797510882559848 1107 68-69 0.20420279673606956 1107 70-71 0.1951585281710848 1107 72-73 0.30198523128037635 1107 74-75 0.2959529447197369 1107 76-77 0.3223166924559564 1107 78-79 0.3598768781812822 1107 80-81 0.3142190351758849 1107 82-83 0.3371274971901066 1107 84-85 0.3941465953150036 1107 86-87 0.3498218258862238 1107 88-89 0.24903832367718692 1107 90-91 0.29269388027679355 1107 92-93 0.1975174839223044 1107 94-95 0.32855896879411617 1107 96-97 0.3331727875745649 1107 98-99 0.30310542802726204 1107 100 0.3080837624701047 1108 1 0.25299214139142023 1108 2 0.1576628060087586 1108 3 0.17185663252899275 1108 4 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2316 60-61 0.21150604065491763 2316 62-63 0.33891100111279115 2316 64-65 -0.07755526891478581 2316 66-67 -0.0012658415703370451 2316 68-69 0.12981644165866157 2316 70-71 -0.11416549981399982 2316 72-73 -0.3292282213608999 2316 74-75 -0.044771079262545754 2316 76-77 0.0177815654028457 2316 78-79 -0.30680267211693746 2316 80-81 -0.022251141114427497 2316 82-83 0.0719003299995542 2316 84-85 0.2282299181976022 2316 86-87 0.17168389195189704 2316 88-89 0.24365056339646074 2316 90-91 0.31838056712761187 2316 92-93 0.33240662746437977 2316 94-95 0.4178907343027163 2316 96-97 0.32509442926626875 2316 98-99 0.28685717948199496 2316 100 0.12494710190372871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 11.0 11 27.0 12 63.0 13 174.0 14 542.0 15 1415.0 16 3135.0 17 6169.0 18 10851.0 19 16400.0 20 22630.0 21 30652.0 22 39270.0 23 44921.0 24 48921.0 25 54751.0 26 64423.0 27 76898.0 28 91442.0 29 108036.0 30 128808.0 31 153384.0 32 186577.0 33 231630.0 34 298766.0 35 402885.0 36 583188.0 37 861371.0 38 782536.0 39 104725.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4736052085108 19.92386604844762 13.569086899066685 24.0334418439749 2 15.072560026199518 21.0763865348264 39.992117162408285 23.858936276565796 3 17.569209217944447 26.384792917752698 30.47437737538896 25.571620488913894 4 11.874165099919326 17.22969675717173 38.411087404178105 32.48505073873084 5 12.456224391986945 38.278011681456405 35.73384797756117 13.531915948995474 6 31.315870332326117 37.93658076626484 17.69135838191439 13.056190519494656 7 27.270695988528942 32.525216942404974 22.44066750378679 17.763419565279296 8 25.721667122126867 36.56756005759467 20.019956046176777 17.69081677410169 9 26.21921310096054 15.298194190435336 20.18875619687816 38.29383651172596 10-11 24.73551821803171 26.42028815272492 28.91529314801668 19.928900481226695 12-13 25.18973581948869 24.0389143150892 28.88921830231725 21.882131563104863 14-15 22.433671163012168 25.556809271460672 26.34867113832569 25.660848427201465 16-17 22.171347593105185 28.51022406282821 26.972575810271632 22.345852533794975 18-19 21.944050413325055 27.637549393590128 28.85950897943146 21.558891213653357 20-21 22.75691411300837 26.863339720134338 28.83999473195128 21.539751434906012 22-23 22.47524262497355 26.80754643669131 28.593377388545456 22.12383354978969 24-25 22.21129000700866 26.914682639922916 28.843316006110303 22.03071134695812 26-27 22.26166708231889 27.084691092320263 28.703748356336583 21.949893469024264 28-29 22.201674004814528 27.024688671733045 28.840821420923398 21.932815902529036 30-31 22.42631591517443 26.98412643735176 28.833427947317464 21.756129700156343 32-33 22.25302828821296 27.098817620421627 28.727392687280904 21.920761404084505 34-35 22.446063718467247 27.164797244547234 28.73184847444109 21.657290562544432 36-37 22.493719198813192 27.062651853008585 28.593709094416404 21.849919853761822 38-39 22.363515652342457 27.263467242562395 28.56779849406085 21.80521861103431 40-41 22.49339708145316 27.13232557656188 28.60659558101377 21.767681760971193 42-43 22.339736765891047 27.07213547964652 28.580769583224523 22.007358171237907 44-45 22.405367722424693 27.33094731146519 28.351777769254817 21.911907196855303 46-47 22.338669274639702 27.25446649256449 28.174766804471723 22.23209742832409 48-49 22.309104867992655 27.338235567678325 28.194174503099322 22.1584850612297 50-51 22.37835566134905 27.49929668759954 27.99732224568511 22.1250254053663 52-53 22.41462122716151 27.55192459415494 27.895468638099736 22.13798554058382 54-55 22.386997978352316 27.468491990458322 27.970906921301342 22.173603109888024 56-57 22.41457470613956 27.56707624020312 27.791685651085967 22.226663402571347 58-59 22.40283502662861 27.465704082211456 27.969983881142547 22.161477010017393 60-61 22.468648554922588 27.601650335934995 27.90442244557922 22.025278663563196 62-63 22.33692275203338 27.597834750624145 27.94789235671196 22.11735014063051 64-65 22.32252160078635 27.414858539178823 27.949560175895193 22.313059684139635 66-67 22.2792709546343 27.546101590634205 27.79939321624442 22.375234238487078 68-69 22.292180328000633 27.49726398182802 27.914218222282962 22.296337467888392 70-71 22.28075425212991 27.33243064610879 27.9101328631276 22.476682238633696 72-73 22.260080993604227 27.52749022604829 27.722481208576617 22.489947571770863 74-75 22.236448403992735 27.626369737290396 27.624463556522223 22.512718302194646 76-77 22.16334331744717 27.560287914514397 27.791306185040828 22.48506258299761 78-79 22.135961464589073 27.497236683974833 27.777225912066545 22.589575939369546 80-81 22.10322212010184 27.69284332084464 27.567692264587635 22.636242294465887 82-83 22.233871848471463 27.67843249570479 27.655970280974056 22.43172537484969 84-85 22.163212180842457 27.637188201324857 27.58018927136119 22.619410346471494 86-87 22.08580460434725 27.608892200313257 27.733262011132187 22.572041184207308 88-89 22.09369760444659 27.552252003950485 27.702657387629483 22.65139300397345 90-91 22.2003996291078 27.63798109772927 27.60804968843654 22.55356958472639 92-93 22.099620929436096 27.595156404794903 27.633504481292594 22.671718184476415 94-95 21.975055707220697 27.55133672635991 27.67459839174078 22.799009174678616 96-97 22.001091223255298 27.611301728742475 27.657376109928055 22.730230938074172 98-99 21.957355385264147 27.49477243557647 27.61416118721755 22.933710991941833 100 21.18337845895467 27.938256757588594 27.78135832560823 23.097006457848504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 498.0 1 413.5 2 705.5 3 1007.0 4 750.0 5 659.0 6 994.5 7 1946.0 8 3106.0 9 3728.0 10 3553.5 11 3209.5 12 3379.0 13 3672.5 14 3914.5 15 4219.0 16 4592.0 17 4997.0 18 5399.0 19 5843.0 20 6631.0 21 7727.5 22 9152.5 23 11298.5 24 14074.0 25 17570.0 26 22404.5 27 28035.0 28 34430.0 29 42231.0 30 51029.5 31 59751.0 32 69644.0 33 81230.0 34 92651.5 35 102906.0 36 115175.5 37 126026.5 38 133425.0 39 141038.0 40 145457.0 41 147906.5 42 151127.5 43 154997.0 44 158478.5 45 161995.0 46 164067.5 47 164075.5 48 165563.5 49 166262.0 50 163618.5 51 157499.0 52 149619.0 53 142721.0 54 135463.0 55 125234.5 56 112784.0 57 100030.0 58 87858.0 59 76136.5 60 64060.5 61 52728.5 62 42588.0 63 33328.5 64 25132.0 65 18881.0 66 14406.0 67 11097.5 68 8651.5 69 6744.0 70 5348.0 71 4171.5 72 3289.0 73 2634.0 74 2087.5 75 1616.5 76 1224.0 77 874.0 78 599.5 79 431.0 80 317.0 81 216.5 82 148.5 83 103.0 84 69.0 85 54.5 86 44.5 87 27.0 88 16.0 89 11.0 90 13.5 91 13.0 92 9.0 93 5.0 94 2.0 95 2.5 96 2.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2515034634543789 2 0.07777960470416193 3 0.0014237778245225982 4 0.0011252437645420535 5 4.1335485228075427E-4 6 7.807813876414247E-4 7 0.0 8 9.6449465532176E-4 9 0.0 10-11 2.6408782229048194E-4 12-13 0.0016189731714329543 14-15 3.444623769006286E-5 16-17 1.7223118845031428E-4 18-19 0.0011252437645420535 20-21 0.0013434032699124515 22-23 1.1482079230020952E-5 24-25 0.0 26-27 2.2964158460041906E-4 28-29 0.001940471389873541 30-31 0.001745276042963185 32-33 0.0029968226790354686 34-35 0.004397636345098025 36-37 0.001653419409123017 38-39 0.0027556990152050286 40-41 0.006602195557262047 42-43 0.01242360972688267 44-45 0.008335989520995212 46-47 0.006257733180361419 48-49 0.0062232869426713565 50-51 0.004983222385829093 52-53 0.0034101775313162227 54-55 0.005580290505790183 56-57 0.003766121987446873 58-59 0.001951953469103562 60-61 0.007968562985634541 62-63 0.01082760071390976 64-65 0.007222227835683179 66-67 0.007865224272564354 68-69 0.015087452108247532 70-71 0.004730616642768633 72-73 0.012963267450693655 74-75 0.008163758332544896 76-77 0.004466528820478151 78-79 0.0159486080504991 80-81 0.0062921794180514825 82-83 0.014547794384436546 84-85 0.023457887866932807 86-87 0.01519079082131772 88-89 0.016350480823549837 90-91 0.030438992038785546 92-93 0.024548685393784794 94-95 0.029198927481943283 96-97 0.01796945399498279 98-99 0.017636473697312183 100 0.0192898931064352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4354612.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.63500285588204 #Duplication Level Percentage of deduplicated Percentage of total 1 77.93296878290928 52.70996566199434 2 14.539585050257514 19.667697527950132 3 4.021804401319517 8.160442565071333 4 1.4751838445236292 3.9909625414922676 5 0.6971209527860501 2.357488881628985 6 0.38097805023560694 1.5460470909428186 7 0.2226852304913965 1.0542921340173845 8 0.15265408080218365 0.825980735281419 9 0.1020901647888139 0.6214381728344005 >10 0.43790838197879317 5.038751765414568 >50 0.02328444805647214 1.074068393192068 >100 0.012813404399421515 1.6311275501748699 >500 5.87495655153657E-4 0.26154322478289044 >1k 2.09819872599315E-4 0.22529897812698058 >5k 4.196397451986298E-5 0.20219575713991042 >10k+ 8.392794903972595E-5 0.6326990199554862 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11851 0.2721482419099566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10441 0.23976877848129755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7124 0.16359666486933852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.592831692008381E-5 0.0 10-11 0.0 0.0 0.0 4.592831692008381E-5 0.0 12-13 0.0 0.0 0.0 4.592831692008381E-5 0.0 14-15 0.0 0.0 0.0 4.592831692008381E-5 5.7410396150104766E-5 16-17 0.0 0.0 0.0 4.592831692008381E-5 9.185663384016762E-5 18-19 0.0 0.0 0.0 4.592831692008381E-5 9.185663384016762E-5 20-21 0.0 0.0 0.0 4.592831692008381E-5 9.185663384016762E-5 22-23 0.0 0.0 0.0 6.889247538012572E-5 9.185663384016762E-5 24-25 0.0 0.0 0.0 1.1482079230020952E-4 9.185663384016762E-5 26-27 0.0 0.0 0.0 3.559444561306495E-4 1.0333871307018857E-4 28-29 0.0 0.0 0.0 9.185663384016763E-4 1.1482079230020952E-4 30-31 0.0 0.0 0.0 0.003192018025945825 1.1482079230020952E-4 32-33 0.0 0.0 0.0 0.008232650807925023 1.1482079230020952E-4 34-35 0.0 0.0 0.0 0.01531709369284795 1.1482079230020952E-4 36-37 0.0 0.0 0.0 0.02848703856968198 1.1482079230020952E-4 38-39 0.0 0.0 0.0 0.048775872569129006 1.2630287153023048E-4 40-41 0.0 0.0 0.0 0.0713496403353502 1.3778495076025143E-4 42-43 0.0 0.0 0.0 0.09457788661768259 1.3778495076025143E-4 44-45 0.0 2.2964158460041904E-5 0.0 0.12254823162201361 1.3778495076025143E-4 46-47 0.0 2.2964158460041904E-5 0.0 0.15592663594368453 1.3778495076025143E-4 48-49 0.0 2.2964158460041904E-5 0.0 0.1908091926444882 1.6074910922029333E-4 50-51 0.0 2.2964158460041904E-5 0.0 0.22898710608430783 1.6074910922029333E-4 52-53 0.0 2.2964158460041904E-5 0.0 0.2664760947703263 2.1815950537039809E-4 54-55 0.0 2.2964158460041904E-5 0.0 0.30713413732383044 2.7556990152050287E-4 56-57 0.0 2.2964158460041904E-5 0.0 0.35361359404695525 2.8705198075052384E-4 58-59 0.0 2.2964158460041904E-5 0.0 0.40516812978974936 2.9853405998054477E-4 60-61 0.0 2.2964158460041904E-5 0.0 0.4606954649461307 2.9853405998054477E-4 62-63 0.0 2.2964158460041904E-5 0.0 0.5158553735671514 2.9853405998054477E-4 64-65 0.0 2.2964158460041904E-5 0.0 0.573150948924956 2.9853405998054477E-4 66-67 0.0 2.2964158460041904E-5 0.0 0.6366812933046618 2.9853405998054477E-4 68-69 0.0 2.2964158460041904E-5 0.0 0.7106373656252267 2.9853405998054477E-4 70-71 0.0 2.2964158460041904E-5 0.0 0.7898637123123713 2.9853405998054477E-4 72-73 0.0 2.2964158460041904E-5 0.0 0.8670806951342622 3.2149821844058667E-4 74-75 0.0 2.2964158460041904E-5 0.0 0.9484197443997306 3.2149821844058667E-4 76-77 0.0 2.2964158460041904E-5 0.0 1.0346042311002681 3.3298029767060765E-4 78-79 0.0 2.2964158460041904E-5 0.0 1.1311340711870541 3.4446237690062857E-4 80-81 0.0 2.2964158460041904E-5 0.0 1.2358735979233053 3.4446237690062857E-4 82-83 0.0 2.2964158460041904E-5 0.0 1.3426684168417302 3.9039069382071237E-4 84-85 0.0 2.2964158460041904E-5 0.0 1.4512085118031182 4.018727730507333E-4 86-87 0.0 2.2964158460041904E-5 0.0 1.5638706732080836 4.1335485228075427E-4 88 0.0 2.2964158460041904E-5 0.0 1.6525697352599955 4.1335485228075427E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6540 0.0 46.92415 1 GTATCAA 11175 0.0 37.374844 1 TCAACGC 13620 0.0 30.15289 4 ATCAACG 13665 0.0 29.950779 3 CAACGCA 13755 0.0 29.890425 5 AACGCAG 14430 0.0 28.6876 6 GTACCTG 3740 0.0 28.485905 1 TATCAAC 14640 0.0 28.328035 2 TACCTGG 3840 0.0 26.571587 2 GTACATG 13700 0.0 25.359467 1 ACGCAGA 16485 0.0 24.94013 7 TACATGG 13920 0.0 24.422382 2 CGCAGAG 16850 0.0 24.372002 8 ACATGGG 13940 0.0 23.460976 3 ACCTGGG 4645 0.0 21.648493 3 GCAGAGT 18980 0.0 21.364328 9 CATGGGG 8645 0.0 20.817492 4 AGAGTAC 17315 0.0 20.040228 10-11 GAGTACT 11805 0.0 19.980549 12-13 CAGAGTA 18670 0.0 18.61095 10-11 >>END_MODULE