FastQCFastQC Report
Mon 6 Jun 2016
ERR1211560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029641
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.034.978641
GTATCAA57450.033.4053041
GTACATG133100.028.8729151
TCAACGC64500.028.6310444
CAACGCA65300.028.2774735
TACATGG133750.028.209632
ATCAACG66800.027.7866233
AACGCAG68350.027.0800136
ACATGGG137250.026.2944453
TATCAAC75250.025.00752
CATGGGG96800.024.7570694
CGTATAC2756.002665E-1122.2139513
ACGCAGA83550.021.8160657
ATGGGGG57350.021.6287655
CGCAGAG89200.020.3301498
TACCTGG34350.019.3111972
GTACCTG36900.019.0401761
GAGTACA96950.018.7250941
GTATAGG11600.018.2921871
AGTACAT95050.018.016322
Produced by FastQC (version 0.11.5)