FastQCFastQC Report
Mon 6 Jun 2016
ERR1211560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029641
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52600.032.988461
GTATCAA77000.029.087261
GTACATG155100.027.0265051
TACATGG157150.025.9739992
TCAACGC87800.024.6185554
CAACGCA89400.024.2305155
ACATGGG161750.024.199443
CATGGGG104650.024.0222574
ATCAACG89950.023.9259363
AACGCAG94250.022.9844976
TATCAAC98100.022.4341852
TACCTGG33600.022.3930992
GTACCTG36550.021.1571121
ATGGGGG57050.020.3441965
ACGCAGA110800.019.381467
CGCAGAG116450.018.5218018
GTGTAGC26800.017.2421421
GTATTAG11000.017.1461731
GAGTACA116600.016.9035421
TAGCCCT29050.016.3370824
Produced by FastQC (version 0.11.5)