FastQCFastQC Report
Mon 6 Jun 2016
ERR1211559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648802
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110570.41743399468891973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104660.39512202120052764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59830.2258756977682741No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.1584112364759616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59100.049.0639571
TACCTGG27200.039.7597582
GTATCAA98650.036.7897341
GTACCTG30000.036.402911
ACCTGGG33100.030.9481283
ATCAACG120100.029.6964593
TCAACGC120000.029.6814864
CAACGCA121300.029.4369685
TATCAAC124450.028.9035632
AACGCAG124350.028.6334366
TATAACG2650.026.615232
GTACATG101350.026.613391
ACGCAGA137750.025.849027
TACATGG104650.025.3410572
CGCAGAG141750.024.9875098
CATGGGG60750.024.6741434
ACATGGG104100.024.1946833
ATGGGGG41050.023.9206735
TAACGCA2905.456968E-1222.6844234
GCAGAGT152650.022.5275279
Produced by FastQC (version 0.11.5)