FastQCFastQC Report
Mon 6 Jun 2016
ERR1211559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648802
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122470.46235996499549603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117320.44291721314012905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70340.2655540127197125No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39510.14916177200107822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66050.047.763541
TACCTGG27050.041.713282
GTACCTG30700.036.7552721
GTATCAA105900.034.585041
ACCTGGG33700.032.350073
TATAACG2800.030.2235072
TCAACGC130750.027.7814544
ATCAACG131450.027.705013
CAACGCA132250.027.4663525
TATCAAC134100.027.2059142
AACGCAG136550.026.7046686
TAACGCA3300.025.6316014
ACGCAGA152050.023.7969597
ATAACGC3950.023.7930473
CGCAGAG153850.023.3658268
GTACATG105850.023.230451
TACATGG107050.022.354322
CATGGGG60650.022.3140724
CCTGGGG28800.022.1903214
GAGTACT107000.021.51977312-13
Produced by FastQC (version 0.11.5)