##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211559_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2648802 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12492515484359 33.0 31.0 34.0 30.0 34.0 2 32.33285613647226 34.0 31.0 34.0 30.0 34.0 3 32.33917219935654 34.0 31.0 34.0 30.0 34.0 4 35.97944164947021 37.0 35.0 37.0 35.0 37.0 5 35.910124275049625 37.0 35.0 37.0 35.0 37.0 6 35.93421101312971 37.0 35.0 37.0 35.0 37.0 7 35.86698137497631 37.0 35.0 37.0 35.0 37.0 8 35.86354283936663 37.0 35.0 37.0 35.0 37.0 9 37.51581884942702 39.0 37.0 39.0 35.0 39.0 10-11 37.47693806483082 39.0 37.0 39.0 34.5 39.0 12-13 37.3887544255856 39.0 37.0 39.0 34.0 39.0 14-15 38.60806470245794 40.0 38.0 41.0 34.0 41.0 16-17 38.53094700925173 40.0 38.0 41.0 34.0 41.0 18-19 38.53646837324949 40.0 38.0 41.0 34.0 41.0 20-21 38.47892405698879 40.0 38.0 41.0 34.0 41.0 22-23 38.361434527760096 40.0 38.0 41.0 34.0 41.0 24-25 38.244688542216444 40.0 38.0 41.0 33.5 41.0 26-27 38.06815118683843 40.0 37.0 41.0 33.0 41.0 28-29 38.09013263354528 40.0 37.5 41.0 33.0 41.0 30-31 37.98897463834594 40.0 37.0 41.0 33.0 41.0 32-33 37.766656586638035 40.0 37.0 41.0 33.0 41.0 34-35 37.62032515076627 40.0 37.0 41.0 33.0 41.0 36-37 37.543067394240865 40.0 37.0 41.0 32.0 41.0 38-39 37.43865660777966 40.0 36.5 41.0 32.0 41.0 40-41 37.26629132717357 40.0 36.0 41.0 31.0 41.0 42-43 37.1067794044251 40.0 36.0 41.0 31.0 41.0 44-45 36.90830854854383 39.0 36.0 41.0 30.5 41.0 46-47 36.627326806609176 39.0 35.0 41.0 30.0 41.0 48-49 36.37936905061231 39.0 35.0 41.0 29.0 41.0 50-51 36.16871702754679 39.0 35.0 40.5 28.0 41.0 52-53 35.963437621989115 39.0 35.0 40.0 28.0 41.0 54-55 35.76699051118204 38.5 35.0 40.0 27.0 41.0 56-57 35.57065816924028 38.0 34.0 40.0 27.0 41.0 58-59 35.31656518682786 38.0 34.0 40.0 26.0 41.0 60-61 34.99967872268293 38.0 34.0 40.0 26.0 41.0 62-63 34.6767555672338 37.0 33.5 40.0 26.0 41.0 64-65 34.36306299980142 37.0 33.0 40.0 25.0 41.0 66-67 34.02300492826568 36.0 33.0 39.0 24.5 41.0 68-69 33.634084389848695 36.0 32.0 39.0 23.5 40.0 70-71 33.24336700138402 35.0 32.0 38.5 23.0 40.0 72-73 32.83599245998757 35.0 31.0 38.0 22.0 40.0 74-75 32.430979929794674 35.0 31.0 37.0 22.0 39.0 76-77 30.87766809297184 33.5 29.5 35.5 20.0 38.0 78-79 31.715857772683652 34.0 30.5 36.0 21.0 38.5 80-81 31.693294176008628 34.0 31.0 36.0 20.5 37.5 82-83 31.412753576900048 34.0 31.0 35.0 20.0 37.0 84-85 31.116213669424894 34.0 30.0 35.0 20.0 37.0 86-87 30.827975816991984 34.0 30.0 35.0 19.5 36.0 88-89 30.55917392088952 34.0 29.5 35.0 19.0 36.0 90-91 30.271224123207396 34.0 29.0 35.0 18.0 35.5 92-93 30.060665916138692 34.0 29.0 35.0 18.0 35.0 94-95 29.838817699473196 34.0 29.0 35.0 17.5 35.0 96-97 29.630142796630327 34.0 29.0 35.0 17.0 35.0 98-99 29.467474352556366 33.5 29.0 35.0 15.5 35.0 100 27.629822085607003 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4021174733768049 1101 2 0.29441767372536276 1101 3 0.33935698670212844 1101 4 0.23131368881416847 1101 5 0.21460047844553287 1101 6 0.23371188310374436 1101 7 0.2973790674549832 1101 8 0.24032703676571998 1101 9 0.3532420436584687 1101 10-11 0.27899534188387776 1101 12-13 0.26996828087041536 1101 14-15 0.34409225941764277 1101 16-17 0.41084412976602636 1101 18-19 0.3789225726776877 1101 20-21 0.3323707073931459 1101 22-23 0.27929433246888635 1101 24-25 0.31037393886504816 1101 26-27 0.282666934801334 1101 28-29 0.35197512129833797 1101 30-31 0.40536267131862047 1101 32-33 0.44582807577756256 1101 34-35 0.357520778192999 1101 36-37 0.42892138315499295 1101 38-39 0.3887993801985985 1101 40-41 0.4010097202890037 1101 42-43 0.4258378604782038 1101 44-45 0.5063604339852645 1101 46-47 0.49948752332537794 1101 48-49 0.4765205900349727 1101 50-51 0.468449619265364 1101 52-53 0.4731191412246716 1101 54-55 0.3416245867780958 1101 56-57 0.3491330483963182 1101 58-59 0.4722881188472101 1101 60-61 0.3707495038981321 1101 62-63 0.44669287367079136 1101 64-65 0.44029175610625515 1101 66-67 0.18769954006398848 1101 68-69 0.3012122938062376 1101 70-71 0.19467270416895133 1101 72-73 0.1382361929813669 1101 74-75 0.09305372091260011 1101 76-77 0.0844036307999474 1101 78-79 0.12311232170488395 1101 80-81 0.15966347991787444 1101 82-83 0.2015818776889624 1101 84-85 0.1628440352088063 1101 86-87 0.15819521835141614 1101 88-89 0.12285424889098095 1101 90-91 0.015432878538732098 1101 92-93 -0.04740257866509623 1101 94-95 -0.09270893673859248 1101 96-97 -0.17306322763009874 1101 98-99 -0.1983130293459503 1101 100 0.001884529986348582 1102 1 0.23943135419348494 1102 2 0.14412646493973824 1102 3 0.2747045987799268 1102 4 0.2192571423045635 1102 5 0.14727838040151653 1102 6 0.1403591273869793 1102 7 0.145914313672165 1102 8 0.21541926473130957 1102 9 0.24505028566952802 1102 10-11 0.17181810421302401 1102 12-13 0.2783330424743369 1102 14-15 0.29329444434690544 1102 16-17 0.2787588661291309 1102 18-19 0.25511440075175784 1102 20-21 0.2856543987869955 1102 22-23 0.1686282012593736 1102 24-25 0.2358617669066163 1102 26-27 0.16166813539005176 1102 28-29 0.18578747547810082 1102 30-31 0.35276264003424274 1102 32-33 0.47804596790131626 1102 34-35 0.5390923635490665 1102 36-37 0.4701334064110725 1102 38-39 0.48908319957399016 1102 40-41 0.48046321118859936 1102 42-43 0.4542796623973189 1102 44-45 0.4763876148262369 1102 46-47 0.5171903029624971 1102 48-49 0.5388097633047053 1102 50-51 0.5326870194321529 1102 52-53 0.5356977330620936 1102 54-55 0.5377808618855795 1102 56-57 0.5085776034420917 1102 58-59 0.5266812248067865 1102 60-61 0.5616949116462919 1102 62-63 0.5889160399543627 1102 64-65 0.5617273179096642 1102 66-67 0.5223801499468053 1102 68-69 0.34876435486764734 1102 70-71 0.3224461453588958 1102 72-73 0.27890332976011223 1102 74-75 0.4273718434031224 1102 76-77 0.41277772185170036 1102 78-79 0.31054191916231844 1102 80-81 0.35735915200886126 1102 82-83 0.25632071155368763 1102 84-85 0.16199133281768496 1102 86-87 0.27051234949287917 1102 88-89 0.39723752515741495 1102 90-91 0.38957236775019766 1102 92-93 0.17195688897233552 1102 94-95 0.25677144298060384 1102 96-97 0.11520350620994435 1102 98-99 0.004447880318377173 1102 100 0.18743909633715816 1103 1 0.28375644117380716 1103 2 0.2440805495395395 1103 3 0.32817212831582054 1103 4 0.18475232614333237 1103 5 0.16605958794433207 1103 6 0.1949149485453816 1103 7 0.18917948093133674 1103 8 0.23961368238007452 1103 9 0.21415898424669422 1103 10-11 0.21242126853490362 1103 12-13 0.23207655999044619 1103 14-15 0.3799769216376063 1103 16-17 0.44480114447458874 1103 18-19 0.3578797195342247 1103 20-21 0.32548873413989554 1103 22-23 0.3459134340192307 1103 24-25 0.2793196524739372 1103 26-27 0.3223805848069148 1103 28-29 0.47740521403685676 1103 30-31 0.5025993591224349 1103 32-33 0.4580217861209306 1103 34-35 0.4417273078695203 1103 36-37 0.5391906762709695 1103 38-39 0.4971673330403732 1103 40-41 0.6001487761846604 1103 42-43 0.5795530052242981 1103 44-45 0.4689341676649974 1103 46-47 0.5346478188278425 1103 48-49 0.5231864474991781 1103 50-51 0.5680293642099699 1103 52-53 0.6356749859830941 1103 54-55 0.6014173640588183 1103 56-57 0.5482063973785856 1103 58-59 0.5948158503460022 1103 60-61 0.6647738700283057 1103 62-63 0.7171417950495993 1103 64-65 0.6701232821120087 1103 66-67 0.6616634509394714 1103 68-69 0.647015543080407 1103 70-71 0.668508137839467 1103 72-73 0.6110622852578516 1103 74-75 0.590694879429492 1103 76-77 0.6233909563401419 1103 78-79 0.6082601120927364 1103 80-81 0.5246439709601987 1103 82-83 0.6444522248563977 1103 84-85 0.571709402844494 1103 86-87 0.47902971817619644 1103 88-89 0.4428231794320432 1103 90-91 0.4499698271145718 1103 92-93 0.5128644875126582 1103 94-95 0.5578088386333349 1103 96-97 0.6410515472974119 1103 98-99 0.5791574771821573 1103 100 0.38059103616989276 1104 1 0.1969738910102592 1104 2 0.11068796894190314 1104 3 0.2006122093296625 1104 4 -0.015082268589807768 1104 5 0.09141593987786933 1104 6 0.1464230589129727 1104 7 0.18136566905257467 1104 8 0.13039422516326482 1104 9 0.15576302008729925 1104 10-11 0.16514494592156836 1104 12-13 0.25409713923227173 1104 14-15 0.3138638016822597 1104 16-17 0.2261502334281218 1104 18-19 0.20782438482511623 1104 20-21 0.17217559837462915 1104 22-23 0.1995866645960902 1104 24-25 0.2486971010647565 1104 26-27 0.2601455656014551 1104 28-29 0.36440233591334703 1104 30-31 0.25140169803196244 1104 32-33 0.25368944969068963 1104 34-35 0.4789806222628883 1104 36-37 0.2876204640707485 1104 38-39 0.37926547669263044 1104 40-41 0.4698996142899219 1104 42-43 0.463641521595342 1104 44-45 0.40835841121436545 1104 46-47 0.35780635276945105 1104 48-49 0.35037357583139084 1104 50-51 0.39089433674023866 1104 52-53 0.3598343355726996 1104 54-55 0.40908810816693375 1104 56-57 0.4322413157359293 1104 58-59 0.46967514807429467 1104 60-61 0.4036799195971881 1104 62-63 0.4148009691730721 1104 64-65 0.4628199987994961 1104 66-67 0.5271015952384985 1104 68-69 0.5698036818100292 1104 70-71 0.3856955536247142 1104 72-73 0.36628710347375915 1104 74-75 0.41754287306719107 1104 76-77 0.35863036279753047 1104 78-79 0.43677203959708066 1104 80-81 0.4472914373611303 1104 82-83 0.3941229713599448 1104 84-85 0.41764541579904346 1104 86-87 0.504831502540906 1104 88-89 0.4016554134571386 1104 90-91 0.4053761599444634 1104 92-93 0.4299739316623281 1104 94-95 0.39808831484599594 1104 96-97 0.3791969242741011 1104 98-99 0.5531798074920466 1104 100 0.4737219865974218 1105 1 0.25552199455813707 1105 2 0.2107098817290094 1105 3 0.25173772975836073 1105 4 0.12406791368474046 1105 5 0.07661347867346535 1105 6 0.18897221327859626 1105 7 0.11611461823483182 1105 8 0.1311448626261651 1105 9 0.12966987082137393 1105 10-11 0.17619677215316898 1105 12-13 0.20121492295305643 1105 14-15 0.31518211728615597 1105 16-17 0.30336330042148774 1105 18-19 0.2637282001401573 1105 20-21 0.22864871038881063 1105 22-23 0.21860659029182017 1105 24-25 0.28574259748899067 1105 26-27 0.3686057067397286 1105 28-29 0.37465683550497175 1105 30-31 0.3650244171100283 1105 32-33 0.40574420924765064 1105 34-35 0.39047568441552727 1105 36-37 0.46763867907503 1105 38-39 0.5099525297215735 1105 40-41 0.46401086401601077 1105 42-43 0.42976815011384417 1105 44-45 0.4193964797017884 1105 46-47 0.5409481359723074 1105 48-49 0.47199452445585877 1105 50-51 0.5247542617387424 1105 52-53 0.40580867022080014 1105 54-55 0.4438228015871175 1105 56-57 0.5422789285369802 1105 58-59 0.5757425000135683 1105 60-61 0.5297896279579106 1105 62-63 0.5013307680734513 1105 64-65 0.6254554596652468 1105 66-67 0.4279188425940177 1105 68-69 0.409200880957421 1105 70-71 0.5050114264508636 1105 72-73 0.5197307104181093 1105 74-75 0.4990885089364383 1105 76-77 0.5716359132069009 1105 78-79 0.720938121092729 1105 80-81 0.71284932945062 1105 82-83 0.689232373439669 1105 84-85 0.674850116091875 1105 86-87 0.6989649140630512 1105 88-89 0.6305789851418311 1105 90-91 0.7871026385595741 1105 92-93 0.5814381665076453 1105 94-95 0.6778476228003392 1105 96-97 0.5340286938312637 1105 98-99 0.6627297362754838 1105 100 0.6295576357139367 1106 1 0.25603448674301177 1106 2 0.21406437602997386 1106 3 0.282950157017531 1106 4 0.19844390560509595 1106 5 0.13184991641693244 1106 6 0.09874353054883045 1106 7 0.1537264347115297 1106 8 0.11290705422910463 1106 9 0.05685690491938544 1106 10-11 0.16121652038574297 1106 12-13 0.22230450237883304 1106 14-15 0.23312099695719724 1106 16-17 0.28235713248035665 1106 18-19 0.19988535038302757 1106 20-21 0.27241193599358127 1106 22-23 0.21876439580329077 1106 24-25 0.27877736279462084 1106 26-27 0.3000557440118925 1106 28-29 0.2993738376210686 1106 30-31 0.25849442504539155 1106 32-33 0.32492599518523235 1106 34-35 0.3461466133339073 1106 36-37 0.4021892189201637 1106 38-39 0.3873804802337659 1106 40-41 0.42191812246837657 1106 42-43 0.38468161257716815 1106 44-45 0.44447703565812446 1106 46-47 0.4817507798909375 1106 48-49 0.4073994684273856 1106 50-51 0.44553213770662126 1106 52-53 0.6253965423412211 1106 54-55 0.46858699506569224 1106 56-57 0.5130661225445792 1106 58-59 0.6401511952475474 1106 60-61 0.6921775108992847 1106 62-63 0.7087315459794752 1106 64-65 0.698644152975497 1106 66-67 0.6439590965256343 1106 68-69 0.68902461971566 1106 70-71 0.6708995859276143 1106 72-73 0.70016478077833 1106 74-75 0.6467877045791965 1106 76-77 0.6579217125143622 1106 78-79 0.7243564890530934 1106 80-81 0.7255887429551606 1106 82-83 0.7134434961732801 1106 84-85 0.6584969563746625 1106 86-87 0.6132847873377081 1106 88-89 0.7333471943949483 1106 90-91 0.7729602589125761 1106 92-93 0.8048945280235387 1106 94-95 0.8938488010814893 1106 96-97 0.9084575881707053 1106 98-99 0.8401955601104305 1106 100 0.8558642653489095 1107 1 0.09192338718523985 1107 2 0.12200257280034776 1107 3 0.15793203372643205 1107 4 0.08327277047930437 1107 5 0.08859541756925893 1107 6 0.1170451255448981 1107 7 0.13313447066423834 1107 8 0.15305703941316295 1107 9 0.1528484722801835 1107 10-11 0.13283074788930804 1107 12-13 0.2255073821880913 1107 14-15 0.19762772124739314 1107 16-17 0.21509665648584786 1107 18-19 0.2135886331761725 1107 20-21 0.22033139437933613 1107 22-23 0.22204025694642127 1107 24-25 0.33221879881342176 1107 26-27 0.21009197106731392 1107 28-29 0.31534741349651796 1107 30-31 0.20863932594455292 1107 32-33 0.2143141187057651 1107 34-35 0.2184007821790317 1107 36-37 0.5148503697601328 1107 38-39 0.3947314579639638 1107 40-41 0.39233767726759083 1107 42-43 0.3398115779317834 1107 44-45 0.37121476027098765 1107 46-47 0.42995593945156685 1107 48-49 0.4849019226993363 1107 50-51 0.546881364861143 1107 52-53 0.5014000967030441 1107 54-55 0.5829953043932932 1107 56-57 0.574586335933212 1107 58-59 0.5772315830884125 1107 60-61 0.4875299179896544 1107 62-63 0.5847818425668336 1107 64-65 0.6698475858807527 1107 66-67 0.7300216632522947 1107 68-69 0.5867160785563854 1107 70-71 0.7557469158115424 1107 72-73 0.6692419305911912 1107 74-75 0.5180035980098552 1107 76-77 0.45962672977359276 1107 78-79 0.5054718610699496 1107 80-81 0.5516149117670892 1107 82-83 0.6895112936356789 1107 84-85 0.5763545124323812 1107 86-87 0.42041001110238696 1107 88-89 0.4847882096116791 1107 90-91 0.34674768259338506 1107 92-93 0.5007829573822455 1107 94-95 0.7330560944308182 1107 96-97 0.6525760005069436 1107 98-99 0.6784657511653087 1107 100 0.7200015796065351 1108 1 0.2839024113442079 1108 2 0.18017528753026113 1108 3 0.18862679907411461 1108 4 0.13176922231020427 1108 5 0.12465909748456028 1108 6 0.15708043755981294 1108 7 0.15258140907764073 1108 8 0.13317366989842583 1108 9 0.16407302935291312 1108 10-11 0.12830488101425175 1108 12-13 0.16133080078284934 1108 14-15 0.24765089415119235 1108 16-17 0.32977162499881985 1108 18-19 0.26937333896271554 1108 20-21 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2316 92-93 0.11760439872951167 2316 94-95 0.24971413407514476 2316 96-97 0.2258605384868524 2316 98-99 0.1640977555037466 2316 100 0.0704631343320159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 4.0 11 14.0 12 29.0 13 105.0 14 338.0 15 849.0 16 2069.0 17 4464.0 18 8231.0 19 12123.0 20 15912.0 21 20471.0 22 25928.0 23 28330.0 24 29079.0 25 30740.0 26 34305.0 27 40387.0 28 48918.0 29 58825.0 30 70052.0 31 85560.0 32 103664.0 33 131595.0 34 174547.0 35 235926.0 36 341907.0 37 532789.0 38 530012.0 39 81615.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94862646759678 19.856436208007043 14.03679062852293 23.15814669587325 2 15.400630914826499 21.562628685845976 40.09580838323353 22.940932016093996 3 17.305620963001473 27.830537896488988 31.153877953670012 23.70996318683953 4 12.107074119349951 18.149322971841396 38.259169686293546 31.4844332225151 5 12.285946847541377 37.83697868963492 35.24950184199538 14.627572620828328 6 28.996475363789575 37.739959166168326 19.295646606140778 13.967918863901321 7 25.85300826562348 33.383242688581475 23.42945980862292 17.334289237172126 8 25.46475257430322 35.52989589527235 21.21025757189644 17.795093958528 9 26.64504681365146 15.59087133796436 21.123414376321353 36.64066747206282 10-11 24.32754505621787 26.41560222319373 29.40187677297133 19.854975947617074 12-13 25.03780954559835 24.600857293221615 29.108630996201303 21.252702164978736 14-15 22.59715901754831 25.751037638902417 27.29881282179642 24.352990521752854 16-17 21.723411962599393 28.353670679771316 27.935081823503303 21.98783553412599 18-19 21.411733437230534 27.242076705733826 30.08378977126004 21.2624000857756 20-21 22.43221871382099 26.64493039377677 29.995565785228834 20.927285107173404 22-23 22.11320855866323 26.53962556273092 29.842685456044933 21.50448042256092 24-25 21.77655097532808 26.59556153897556 29.939904885266955 21.6879826004294 26-27 21.69486342387442 26.585686523790514 29.936263530904117 21.78318652143095 28-29 21.735110531220737 26.904365090825898 29.968591158974718 21.391933218978647 30-31 22.314196076654735 26.652339207780244 30.03148643138676 21.001978284178257 32-33 22.089577539658748 26.777121192786556 29.822627667229707 21.310673600324986 34-35 22.16135146053332 27.005330937180265 29.734548021852312 21.0987695804341 36-37 22.137971456589796 26.986632487312516 29.539399854083353 21.33599620201433 38-39 22.085120022470946 27.177571039062144 29.376812644737864 21.36049629372905 40-41 22.22615922943561 26.891298832313442 29.493958445339825 21.388583492911124 42-43 21.93865107112383 27.131713873639857 29.202377086935837 21.727257968300474 44-45 21.852296379804347 27.289423480246118 29.241932457396523 21.616347682553013 46-47 21.848939696357082 27.049527402071543 28.9460166665125 22.15551623505888 48-49 21.85911519500589 27.148421350310603 29.00958293956605 21.982880515117454 50-51 21.985810113470386 27.4413255573324 28.805643333315086 21.767220995882127 52-53 22.082983632292326 27.523350213369547 28.49320664433866 21.900459509999468 54-55 21.89082947835138 27.417387985292613 28.72982481839458 21.96195771796143 56-57 22.008024183546688 27.51550452422149 28.344765800230526 22.1317054920013 58-59 21.991129686103395 27.53591589907965 28.55871085023063 21.914243564586325 60-61 22.29730609850096 27.620357748367198 28.490763116348354 21.591573036783483 62-63 22.097907246759867 27.57969359147418 28.65816475829974 21.664234403466214 64-65 21.98728012955836 27.30364306437464 28.575195931725506 22.13388087434149 66-67 21.917078168440955 27.366213075514896 28.433031071380903 22.28367768466325 68-69 21.77708521610951 27.484306448972905 28.524344614541164 22.214263720376422 70-71 21.80213142785732 27.33142619609182 28.542119806829675 22.32432256922119 72-73 21.699739730964087 27.771916202696094 28.110339573031922 22.418004493307894 74-75 21.865256472623408 27.71643667765502 27.979186876949885 22.43911997277169 76-77 21.6880090186822 27.504960879276208 28.299116857777555 22.50791324426403 78-79 21.653270245749347 27.479137036154665 28.303518132079937 22.564074586016048 80-81 21.637986934841273 27.678352819693824 27.99547693681398 22.688183308650924 82-83 21.882884013959597 27.73065947478767 27.9788699222065 22.40758658904623 84-85 21.800743945858322 27.872727183476563 27.777987555086998 22.548541315578117 86-87 21.544816835836023 27.856310615820007 27.949166683816777 22.64970586452719 88-89 21.493576143473273 27.627576995745624 28.054562800709686 22.824284060071417 90-91 21.918140579935898 27.513601540705494 28.017635035945077 22.55062284341353 92-93 21.70173681798449 27.667291259222782 27.899799819439057 22.73117210335367 94-95 21.49882258299043 27.621070004853188 27.847998957603547 23.032108454552834 96-97 21.456527629220048 27.570669685128944 27.91462711197167 23.05817557367934 98-99 21.372240387884883 27.470899854492192 27.83847368408135 23.318386073541575 100 20.614403083645545 28.055684149871578 28.03703063179541 23.292882134687463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1091.0 1 865.0 2 1606.5 3 2368.5 4 1570.0 5 1090.0 6 1455.5 7 2493.5 8 3934.0 9 4870.5 10 4341.5 11 3444.0 12 3443.0 13 3686.0 14 3961.0 15 4200.0 16 4550.5 17 4853.0 18 5119.5 19 5426.0 20 5942.0 21 6815.5 22 7771.0 23 9188.0 24 11178.0 25 13566.0 26 16729.0 27 20482.0 28 24565.0 29 29083.0 30 34137.5 31 39198.0 32 44380.5 33 50286.5 34 56058.0 35 61378.0 36 67894.0 37 73807.0 38 77678.0 39 81157.5 40 83149.5 41 84434.5 42 85971.5 43 87764.5 44 89980.0 45 92494.5 46 94419.5 47 95960.5 48 98263.0 49 100154.0 50 101450.5 51 98834.5 52 92934.0 53 87632.0 54 82416.0 55 75796.5 56 68956.5 57 62168.5 58 54234.5 59 45924.5 60 37474.5 61 30181.0 62 24316.5 63 19022.0 64 14333.5 65 10808.0 66 8133.0 67 6200.5 68 4796.0 69 3615.0 70 2812.0 71 2188.0 72 1693.5 73 1344.0 74 1077.0 75 836.5 76 571.5 77 397.0 78 285.5 79 183.5 80 125.5 81 94.0 82 65.5 83 50.0 84 40.5 85 31.5 86 21.0 87 15.0 88 9.0 89 4.0 90 5.5 91 4.5 92 2.0 93 2.0 94 2.0 95 2.0 96 1.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0031712449628171525 2 0.06991840084687341 3 7.928112407042881E-4 4 7.550583244802745E-4 5 0.001661128313856604 6 6.79552492032247E-4 7 0.0 8 0.0010193287380483706 9 7.550583244802745E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.11687698816295E-4 18-19 0.0011325874867204118 20-21 0.003039109756033105 22-23 1.8876458112006863E-5 24-25 3.7752916224013726E-5 26-27 1.6988812300806175E-4 28-29 9.626993637123499E-4 30-31 0.0015856224814085763 32-33 0.003020233297921098 34-35 0.004228326617089537 36-37 0.001113711028608405 38-39 0.001812139978752659 40-41 0.005511925768706003 42-43 0.006871030752770497 44-45 0.005021137857793826 46-47 0.0074562009542427105 48-49 0.009098452809987306 50-51 0.008513282608515095 52-53 0.004190573700865523 54-55 0.002453939554560892 56-57 0.00222742205721681 58-59 0.0030013568398090914 60-61 0.00787148303270686 62-63 0.014232849416453175 64-65 0.00420945015897753 66-67 0.010249916754819727 68-69 0.012892620890500686 70-71 0.005851702014722127 72-73 0.007418448038018697 74-75 0.0033411330858252146 76-77 0.0029447274654730705 78-79 0.007890359490818868 80-81 0.00583282555661012 82-83 0.0167811712615741 84-85 0.018536681865990737 86-87 0.023312425768328475 88-89 0.022179838281608064 90-91 0.02508681283085712 92-93 0.03558212354113294 94-95 0.039904832448782505 96-97 0.02470928366861698 98-99 0.018517805407878733 100 0.019178481441798973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2648802.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.88095090373075 #Duplication Level Percentage of deduplicated Percentage of total 1 80.52220048669588 56.2696793887116 2 13.580116344528479 18.979828870778924 3 3.155459524452959 6.615195363210205 4 1.0558354775417982 2.9513114867406207 5 0.5181849103980363 1.8105627141289646 6 0.28735921595277103 1.2048561157038096 7 0.19074934483050915 0.9330821920713732 8 0.13225941647805678 0.7393931031567331 9 0.09674957555107404 0.6084857105137255 >10 0.42381216659230564 5.074415357116189 >50 0.023951057878626718 1.1540234409125807 >100 0.012299926362729419 1.5775410152275418 >500 3.6090096591666576E-4 0.16380456690872605 >1k 4.812012878882678E-4 0.6142356007786831 >5k 6.0150160986033476E-5 0.29566367042203906 >10k+ 1.2030032197206695E-4 1.007921403618151 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12247 0.46235996499549603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11732 0.44291721314012905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7034 0.2655540127197125 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3951 0.14916177200107822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.7752916224013726E-5 0.0 0.0 0.0 3 0.0 7.550583244802745E-5 0.0 0.0 0.0 4 0.0 7.550583244802745E-5 0.0 0.0 0.0 5 0.0 7.550583244802745E-5 0.0 0.0 0.0 6 0.0 7.550583244802745E-5 0.0 0.0 0.0 7 0.0 7.550583244802745E-5 0.0 0.0 0.0 8 0.0 7.550583244802745E-5 0.0 3.7752916224013726E-5 0.0 9 0.0 7.550583244802745E-5 0.0 7.550583244802745E-5 0.0 10-11 0.0 7.550583244802745E-5 0.0 7.550583244802745E-5 0.0 12-13 0.0 7.550583244802745E-5 0.0 1.1325874867204117E-4 0.0 14-15 0.0 7.550583244802745E-5 0.0 1.1325874867204117E-4 5.6629374336020585E-5 16-17 0.0 7.550583244802745E-5 0.0 1.1325874867204117E-4 7.550583244802745E-5 18-19 0.0 1.1325874867204117E-4 0.0 1.1325874867204117E-4 7.550583244802745E-5 20-21 0.0 1.1325874867204117E-4 0.0 1.510116648960549E-4 7.550583244802745E-5 22-23 0.0 1.1325874867204117E-4 0.0 1.6988812300806175E-4 9.438229056003432E-5 24-25 0.0 1.1325874867204117E-4 0.0 2.6427041356809605E-4 1.1325874867204117E-4 26-27 0.0 1.1325874867204117E-4 0.0 5.47417285248199E-4 1.1325874867204117E-4 28-29 0.0 1.1325874867204117E-4 0.0 0.0015478695651845627 1.1325874867204117E-4 30-31 0.0 1.1325874867204117E-4 0.0 0.004039562035969469 1.1325874867204117E-4 32-33 0.0 1.1325874867204117E-4 0.0 0.010816210498179931 1.1325874867204117E-4 34-35 0.0 1.510116648960549E-4 0.0 0.02099062142055163 1.510116648960549E-4 36-37 0.0 1.510116648960549E-4 0.0 0.03794168080513379 1.510116648960549E-4 38-39 0.0 1.510116648960549E-4 0.0 0.06710580858818439 1.8876458112006863E-4 40-41 0.0 1.510116648960549E-4 0.0 0.10349961982813363 2.2651749734408234E-4 42-43 0.0 2.6427041356809605E-4 0.0 0.1392138785760506 2.2651749734408234E-4 44-45 0.0 3.020233297921098E-4 0.0 0.18468726616787512 2.2651749734408234E-4 46-47 0.0 3.020233297921098E-4 0.0 0.24171304612424788 2.453939554560892E-4 48-49 0.0 3.020233297921098E-4 0.0 0.30326917602750225 2.6427041356809605E-4 50-51 0.0 3.020233297921098E-4 0.0 0.37092240190093484 2.6427041356809605E-4 52-53 0.0 3.020233297921098E-4 0.0 0.4426340662684489 2.6427041356809605E-4 54-55 0.0 3.020233297921098E-4 0.0 0.5272005986102397 2.6427041356809605E-4 56-57 0.0 3.020233297921098E-4 0.0 0.6235271643558107 2.6427041356809605E-4 58-59 0.0 3.020233297921098E-4 0.0 0.7420524448411017 2.6427041356809605E-4 60-61 0.0 3.9640562035214407E-4 0.0 0.8622954830145855 2.6427041356809605E-4 62-63 0.0 4.15282078464151E-4 0.0 0.990202363181544 2.6427041356809605E-4 64-65 0.0 4.15282078464151E-4 0.0 1.1161649681629657 2.6427041356809605E-4 66-67 0.0 4.15282078464151E-4 0.0 1.2716881065477903 2.6427041356809605E-4 68-69 0.0 4.15282078464151E-4 0.0 1.4519016521431198 2.6427041356809605E-4 70-71 0.0 4.15282078464151E-4 0.0 1.6501610916935279 3.020233297921098E-4 72-73 0.0 4.15282078464151E-4 0.0 1.840247024881437 4.15282078464151E-4 74-75 0.0 4.15282078464151E-4 0.0 2.0361280307097323 4.15282078464151E-4 76-77 0.0 4.15282078464151E-4 0.0 2.2665718313411123 4.341585365761578E-4 78-79 0.0 4.15282078464151E-4 0.0 2.52493391352015 4.530349946881647E-4 80-81 0.0 4.15282078464151E-4 0.0 2.7985292973955773 4.530349946881647E-4 82-83 0.0 4.15282078464151E-4 0.0 3.083526061970657 4.530349946881647E-4 84-85 0.0 4.15282078464151E-4 0.0 3.383623993035342 4.530349946881647E-4 86-87 0.0 4.15282078464151E-4 0.0 3.7040707459447706 4.530349946881647E-4 88 0.0 4.15282078464151E-4 0.0 3.9573361844335664 4.530349946881647E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6605 0.0 47.76354 1 TACCTGG 2705 0.0 41.71328 2 GTACCTG 3070 0.0 36.755272 1 GTATCAA 10590 0.0 34.58504 1 ACCTGGG 3370 0.0 32.35007 3 TATAACG 280 0.0 30.223507 2 TCAACGC 13075 0.0 27.781454 4 ATCAACG 13145 0.0 27.70501 3 CAACGCA 13225 0.0 27.466352 5 TATCAAC 13410 0.0 27.205914 2 AACGCAG 13655 0.0 26.704668 6 TAACGCA 330 0.0 25.631601 4 ACGCAGA 15205 0.0 23.796959 7 ATAACGC 395 0.0 23.793047 3 CGCAGAG 15385 0.0 23.365826 8 GTACATG 10585 0.0 23.23045 1 TACATGG 10705 0.0 22.35432 2 CATGGGG 6065 0.0 22.314072 4 CCTGGGG 2880 0.0 22.190321 4 GAGTACT 10700 0.0 21.519773 12-13 >>END_MODULE