FastQCFastQC Report
Mon 6 Jun 2016
ERR1211558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452782
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55280.3805113224145123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52880.3639912939449966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.19872217579788293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.055.806271
GTATCAA51800.037.727781
TCAACGC61450.030.9672814
CAACGCA61750.030.8897345
TATCAAC62750.030.7993852
ATCAACG62100.030.7198683
AACGCAG63850.029.87076
TACCTGG11150.028.6779442
ACGCAGA71500.026.6747447
CGCAGAG73600.025.8515958
GTACCTG13500.025.1154861
GTGGTAT14250.023.4631541
GCAGAGT80300.023.2874839
GTACATG52400.022.6470761
GAGTACT50800.022.52384812-13
GTACCGT1503.3048993E-521.9223466
CAGAGTA77000.021.8447610-11
TACATGG53450.021.4662462
ACATGGG54450.020.8834553
CATGGGG33750.020.7435194
Produced by FastQC (version 0.11.5)