##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211558_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1452782
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30090818856511	31.0	31.0	34.0	28.0	34.0
2	31.342657742180176	31.0	31.0	34.0	28.0	34.0
3	31.429240588057947	33.0	31.0	34.0	28.0	34.0
4	34.97175419298973	37.0	35.0	37.0	32.0	37.0
5	35.055525192355084	37.0	35.0	37.0	32.0	37.0
6	35.176139985214576	37.0	35.0	37.0	32.0	37.0
7	35.115264368638925	37.0	35.0	37.0	32.0	37.0
8	35.086198755215854	37.0	35.0	37.0	32.0	37.0
9	36.648860599869764	39.0	37.0	39.0	32.0	39.0
10-11	36.63430198061375	39.0	37.0	39.0	32.0	39.0
12-13	36.57759250871776	39.0	37.0	39.0	32.0	39.0
14-15	37.75373352643411	40.0	38.0	41.0	32.0	41.0
16-17	37.73387714054827	40.0	38.0	41.0	32.0	41.0
18-19	37.75086041814945	40.0	38.0	41.0	32.0	41.0
20-21	37.71710862331719	40.0	37.5	41.0	32.0	41.0
22-23	37.56780714518764	40.0	37.0	41.0	32.0	41.0
24-25	37.47954235391133	40.0	37.0	41.0	31.0	41.0
26-27	37.33064148647216	40.0	37.0	41.0	31.0	41.0
28-29	37.16997870292997	40.0	36.5	41.0	30.5	41.0
30-31	36.98627220050909	39.5	36.0	41.0	30.0	41.0
32-33	36.80607413913444	39.0	36.0	41.0	30.0	41.0
34-35	36.63555578194113	39.0	35.0	41.0	30.0	41.0
36-37	36.461343133381334	39.0	35.0	41.0	29.5	41.0
38-39	36.326919317557625	39.0	35.0	40.0	29.0	41.0
40-41	36.27855246003874	39.0	35.0	40.0	29.0	41.0
42-43	36.21066890971942	39.0	35.0	40.0	28.0	41.0
44-45	36.02037022760469	39.0	35.0	40.0	27.5	41.0
46-47	35.94720370984773	39.0	35.0	40.0	27.0	41.0
48-49	35.8903576035496	39.0	35.0	41.0	27.0	41.0
50-51	34.91088924559914	38.0	34.0	40.0	25.0	40.5
52-53	35.11087967774931	38.0	34.0	39.5	26.0	40.5
54-55	35.68782756118949	39.0	35.0	40.5	26.0	41.0
56-57	35.64906503522208	39.0	35.0	41.0	26.0	41.0
58-59	35.48769154628843	38.5	34.0	40.5	26.0	41.0
60-61	35.296929959209294	38.0	34.0	40.0	26.0	41.0
62-63	35.04371578117019	38.0	34.0	40.0	26.0	41.0
64-65	34.760521193131524	37.0	34.0	40.0	25.5	41.0
66-67	34.452981589804935	37.0	33.5	40.0	25.0	41.0
68-69	34.17501903244947	36.0	33.0	39.0	25.0	41.0
70-71	33.82489423740107	36.0	33.0	39.0	24.5	41.0
72-73	33.483591481722655	35.0	33.0	39.0	24.0	40.0
74-75	33.118480955848845	35.0	32.0	37.5	23.5	40.0
76-77	32.78482697335181	35.0	32.0	37.0	23.0	39.0
78-79	32.472257365523525	35.0	32.0	36.5	23.0	39.0
80-81	32.13383494564222	35.0	31.5	36.0	22.0	38.5
82-83	31.839712702938225	35.0	31.0	36.0	21.0	37.0
84-85	31.582149283237264	35.0	31.0	35.0	20.5	37.0
86-87	31.298320050771554	34.5	31.0	35.0	20.0	36.5
88-89	31.07735331247221	34.0	31.0	35.0	20.0	36.0
90-91	30.84602094464276	34.0	30.0	35.0	19.5	36.0
92-93	30.703321282890343	34.0	30.0	35.0	19.0	35.5
94-95	30.5458217406328	34.0	30.0	35.0	19.0	35.0
96-97	30.447455640281888	34.0	30.0	35.0	18.0	35.0
98-99	30.27432574192136	34.0	29.5	35.0	18.0	35.0
100	28.89461529672036	33.0	27.0	35.0	13.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5122673362859658
1101	2	0.5285214183267506
1101	3	0.5269234118128736
1101	4	0.648998366094304
1101	5	0.48026203240834775
1101	6	0.5215259486360608
1101	7	0.5824383090640737
1101	8	0.5948009570107615
1101	9	0.5612903332245835
1101	10-11	0.6434802946706597
1101	12-13	0.6482214731599711
1101	14-15	0.7734782467336814
1101	16-17	0.6821599558635043
1101	18-19	0.6246120965370139
1101	20-21	0.7013890809424979
1101	22-23	0.7269976912337697
1101	24-25	0.6342693523103478
1101	26-27	0.5881792653903162
1101	28-29	0.596849315796149
1101	30-31	0.6953982735391335
1101	32-33	0.7088697129520867
1101	34-35	0.6178178927553262
1101	36-37	0.6256217500215016
1101	38-39	0.5495742509412551
1101	40-41	0.45989705167067996
1101	42-43	0.48848017813571687
1101	44-45	0.39664671249165906
1101	46-47	0.3819327543094033
1101	48-49	0.30171078555788
1101	50-51	0.335583667070388
1101	52-53	0.5314221506488721
1101	54-55	0.6797060163472182
1101	56-57	0.7039030606684449
1101	58-59	0.7555230982590899
1101	60-61	0.490721216478228
1101	62-63	0.5582715745134834
1101	64-65	0.5865864786426016
1101	66-67	0.5429576021880109
1101	68-69	0.5225106164508944
1101	70-71	0.47839211410519766
1101	72-73	0.501121456925901
1101	74-75	0.4919961289479531
1101	76-77	0.4294181277325677
1101	78-79	0.3272511765459001
1101	80-81	0.3746726548409782
1101	82-83	0.31279641767521227
1101	84-85	0.10838684509038288
1101	86-87	0.10578348888739342
1101	88-89	0.18323717960199204
1101	90-91	0.013176495820481193
1101	92-93	0.08447234125940639
1101	94-95	-0.15273420019070372
1101	96-97	-0.14627723699679152
1101	98-99	-0.26597865526136033
1101	100	-0.054085674371030734
1102	1	0.4751224547477584
1102	2	0.4524688954000453
1102	3	0.44095881782626734
1102	4	0.45560322790692
1102	5	0.4087485600999514
1102	6	0.38917448971150037
1102	7	0.42554297638328364
1102	8	0.40743362130169913
1102	9	0.4084125400416312
1102	10-11	0.4687839619726901
1102	12-13	0.44116569274862627
1102	14-15	0.6058732706888534
1102	16-17	0.5546049433464972
1102	18-19	0.5058921618211016
1102	20-21	0.4632061421582705
1102	22-23	0.5886979444111802
1102	24-25	0.5503988289252177
1102	26-27	0.4421432725369172
1102	28-29	0.4870882104464158
1102	30-31	0.3324524785911791
1102	32-33	0.43342411399078173
1102	34-35	0.571223468631274
1102	36-37	0.46336030309505816
1102	38-39	0.5190298261825816
1102	40-41	0.4870955178232279
1102	42-43	0.5036301321815202
1102	44-45	0.377159107433485
1102	46-47	0.4379306174950486
1102	48-49	0.6611359138078043
1102	50-51	0.447893718683936
1102	52-53	0.5718200371289228
1102	54-55	0.43730208526582004
1102	56-57	0.5733076420521783
1102	58-59	0.4638710839270601
1102	60-61	0.5498526260813534
1102	62-63	0.6165279504255636
1102	64-65	0.5408025059448534
1102	66-67	0.4099657335804352
1102	68-69	0.5734036809129535
1102	70-71	0.45204273674542605
1102	72-73	0.622279838770595
1102	74-75	0.5791289899721548
1102	76-77	0.5325207269260659
1102	78-79	0.4949114021582659
1102	80-81	0.339317784628939
1102	82-83	0.31006959020244196
1102	84-85	0.22680645360986418
1102	86-87	0.43635733030769686
1102	88-89	0.3857451366596365
1102	90-91	0.1953688509228897
1102	92-93	0.2364006258275282
1102	94-95	0.26678024570466263
1102	96-97	0.22513443425036428
1102	98-99	0.16633353167330966
1102	100	0.1359075090269215
1103	1	0.14360646203806482
1103	2	0.09426765494071176
1103	3	0.15602007967180143
1103	4	0.11005567503330127
1103	5	0.04540722657811358
1103	6	0.07624032800895009
1103	7	0.23697153926293169
1103	8	0.19685549454266038
1103	9	0.25468699176317244
1103	10-11	0.1620188095820012
1103	12-13	0.2598299567406315
1103	14-15	0.22457732991492918
1103	16-17	0.34339566567113167
1103	18-19	0.3022185455750659
1103	20-21	0.18920791424304184
1103	22-23	0.23071905608206578
1103	24-25	0.32968282888482037
1103	26-27	0.36945338774486913
1103	28-29	0.2025240486728137
1103	30-31	0.28356530518606604
1103	32-33	0.28630461196978274
1103	34-35	0.41171171575967946
1103	36-37	0.36962924931052754
1103	38-39	0.2489944779911113
1103	40-41	0.10441609424074016
1103	42-43	0.2165970950309415
1103	44-45	0.43724336561329125
1103	46-47	0.35440887392521603
1103	48-49	0.3658620092707352
1103	50-51	0.3824709522943053
1103	52-53	0.3652193766926146
1103	54-55	0.411591895554011
1103	56-57	0.40666865252197937
1103	58-59	0.43060728004873994
1103	60-61	0.20098549989941716
1103	62-63	0.16203065885778045
1103	64-65	0.2881209093331307
1103	66-67	0.34332049799377273
1103	68-69	0.23610054857173424
1103	70-71	0.22567368157928058
1103	72-73	0.21683017319642772
1103	74-75	0.1621351533712314
1103	76-77	0.16036759157402258
1103	78-79	0.035308052925536515
1103	80-81	0.10304966498740953
1103	82-83	0.1481699362788511
1103	84-85	0.0632345770214684
1103	86-87	0.12577123702153514
1103	88-89	0.3112839151329574
1103	90-91	0.11173172361516492
1103	92-93	-0.038829982691716225
1103	94-95	0.023616591918710128
1103	96-97	0.11328925566364134
1103	98-99	0.21570767307430572
1103	100	0.08012124833148704
1104	1	0.5460120179950785
1104	2	0.5498310324317188
1104	3	0.5889568253374229
1104	4	0.5107964796618205
1104	5	0.4669381917298381
1104	6	0.4888251487402897
1104	7	0.5699241360030527
1104	8	0.6351672307869904
1104	9	0.6986670411656846
1104	10-11	0.7447802610120604
1104	12-13	0.6005484144621605
1104	14-15	0.6798755515642299
1104	16-17	0.7913347928829992
1104	18-19	0.5847309253419439
1104	20-21	0.6766476859875681
1104	22-23	0.7652025247322953
1104	24-25	0.6742280778749006
1104	26-27	0.7922019329814276
1104	28-29	0.7952248619727982
1104	30-31	0.7380927294874695
1104	32-33	0.5984396584623894
1104	34-35	0.6437799934860493
1104	36-37	0.7885871344042243
1104	38-39	0.6493510494658565
1104	40-41	0.6392849615649112
1104	42-43	0.46165295841333176
1104	44-45	0.6230550610644272
1104	46-47	0.8733866505633685
1104	48-49	0.8796001899248083
1104	50-51	0.865328869944868
1104	52-53	0.8925249892938965
1104	54-55	0.8141845175004434
1104	56-57	0.9606440864645762
1104	58-59	0.9767550804401992
1104	60-61	0.8244779966689819
1104	62-63	0.7745435669938132
1104	64-65	0.7973982557389405
1104	66-67	0.7811743790594363
1104	68-69	0.6650865693226535
1104	70-71	0.5825600410303693
1104	72-73	0.7430903141932887
1104	74-75	0.7491295798537152
1104	76-77	0.8301520247310563
1104	78-79	0.6308261227619454
1104	80-81	0.6171219614273653
1104	82-83	0.6819331134557949
1104	84-85	0.5475097672149367
1104	86-87	0.48849685259101605
1104	88-89	0.6559884518814236
1104	90-91	0.6198765729095221
1104	92-93	0.7503196460124002
1104	94-95	0.5539012741085543
1104	96-97	0.6160740718883027
1104	98-99	0.6899708506397495
1104	100	0.6908953946073773
1105	1	0.4376565421751266
1105	2	0.5009420076298703
1105	3	0.4980752259136949
1105	4	0.555953561066886
1105	5	0.3364379640756283
1105	6	0.2979014162985436
1105	7	0.39000482299776706
1105	8	0.4195911523742737
1105	9	0.6157578695325299
1105	10-11	0.5203171738397501
1105	12-13	0.5320811275793531
1105	14-15	0.6106250662417025
1105	16-17	0.49141849261502557
1105	18-19	0.5205618858614898
1105	20-21	0.6265292151895991
1105	22-23	0.6111168678536245
1105	24-25	0.5726569240418158
1105	26-27	0.4264137170882165
1105	28-29	0.591493598140822
1105	30-31	0.6710598906507954
1105	32-33	0.6516943208432622
1105	34-35	0.5327844083920752
1105	36-37	0.5683583022816521
1105	38-39	0.5262807303648813
1105	40-41	0.5820033114003422
1105	42-43	0.59331127775895
1105	44-45	0.6347532029077243
1105	46-47	0.5778654356827175
1105	48-49	0.6191428201577409
1105	50-51	0.6048550773616412
1105	52-53	0.6212025108757473
1105	54-55	0.7132153448252936
1105	56-57	0.7462498845115064
1105	58-59	0.6941756109990962
1105	60-61	0.7567372778253301
1105	62-63	0.8191111259290693
1105	64-65	0.7340701777067764
1105	66-67	0.6573627680151475
1105	68-69	0.6061297919095736
1105	70-71	0.6935120207159144
1105	72-73	0.5674041236297285
1105	74-75	0.6087971820546016
1105	76-77	0.6258578547175304
1105	78-79	0.5824745204613961
1105	80-81	0.701684174452808
1105	82-83	0.6229989882029336
1105	84-85	0.5405476257703192
1105	86-87	0.4533288075404016
1105	88-89	0.5049042062323359
1105	90-91	0.4947488713562649
1105	92-93	0.5404915532563379
1105	94-95	0.529479413867854
1105	96-97	0.5346248319815423
1105	98-99	0.579855868274457
1105	100	0.7874672166791896
1106	1	0.3540243388710458
1106	2	0.3900687177417339
1106	3	0.3938282647072384
1106	4	0.22577046262682643
1106	5	0.2702507641157794
1106	6	0.2455317489016835
1106	7	0.28905190482778664
1106	8	0.23385676083609042
1106	9	0.2710266453587451
1106	10-11	0.3467230179477383
1106	12-13	0.40399268835979996
1106	14-15	0.42490441814103264
1106	16-17	0.40866347914845136
1106	18-19	0.47066344787288017
1106	20-21	0.43874027933400583
1106	22-23	0.4157477290811613
1106	24-25	0.47079952833956895
1106	26-27	0.34149142328061544
1106	28-29	0.48409560532007134
1106	30-31	0.46569429330323686
1106	32-33	0.4977676215668083
1106	34-35	0.5532551589709911
1106	36-37	0.4435698815061926
1106	38-39	0.39852512134934415
1106	40-41	0.42038028711908026
1106	42-43	0.3727346827931939
1106	44-45	0.3297859262316152
1106	46-47	0.4162906130228521
1106	48-49	0.3615880127232174
1106	50-51	0.37409021323643543
1106	52-53	0.3831516330684579
1106	54-55	0.47375546590298967
1106	56-57	0.46163763468688757
1106	58-59	0.39074301817811374
1106	60-61	0.26958261156977414
1106	62-63	0.37836207273240063
1106	64-65	0.6313204544677404
1106	66-67	0.5157784723424186
1106	68-69	0.6297091487087343
1106	70-71	0.40843807106310237
1106	72-73	0.4375286391735571
1106	74-75	0.5458255481112602
1106	76-77	0.5614071082098562
1106	78-79	0.473461773323514
1106	80-81	0.4336032881484897
1106	82-83	0.2871275008960943
1106	84-85	0.4914883341907448
1106	86-87	0.5218179336928124
1106	88-89	0.5579741914013745
1106	90-91	0.5201160701130618
1106	92-93	0.4372863047769755
1106	94-95	0.5620286542142807
1106	96-97	0.5171921778130333
1106	98-99	0.5436526661730277
1106	100	0.6615167783033691
1107	1	0.4523514796197823
1107	2	0.47064218744592523
1107	3	0.42313919370978326
1107	4	0.42805754860626166
1107	5	0.4064102349964145
1107	6	0.2578199752245851
1107	7	0.2650140388616222
1107	8	0.36517797590076384
1107	9	0.20848536943746154
1107	10-11	0.3872404877634992
1107	12-13	0.3810922944900952
1107	14-15	0.45433779049762535
1107	16-17	0.4275127823493676
1107	18-19	0.3553546239935983
1107	20-21	0.4818081396148557
1107	22-23	0.5757258164380374
1107	24-25	0.5909398398092094
1107	26-27	0.6872158736391683
1107	28-29	0.7147659631992269
1107	30-31	0.6383552150453298
1107	32-33	0.5872552545829492
1107	34-35	0.7104086401770644
1107	36-37	0.7800792901601739
1107	38-39	0.5945023751889806
1107	40-41	0.6476540212352759
1107	42-43	0.7829252514143903
1107	44-45	0.6319096723665467
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2314	1	-0.38345926890529114
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2314	96-97	-0.4249908016510595
2314	98-99	-0.3960843653811601
2314	100	-0.21565730373210457
2315	1	-0.3042388654661359
2315	2	-0.27071895878247787
2315	3	-0.31200033899612123
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2315	5	-0.24389177371267579
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2315	9	-0.520748605371729
2315	10-11	-0.3992580841333009
2315	12-13	-0.3162756644929132
2315	14-15	-0.30276760820068915
2315	16-17	-0.37869244310364536
2315	18-19	-0.25079031677523744
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2315	24-25	-0.13036347994543718
2315	26-27	-0.2871485033800809
2315	28-29	-0.16881436175277287
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2315	100	0.09831012351657265
2316	1	-0.601578362547901
2316	2	-0.9571757579970104
2316	3	-0.5056709057336981
2316	4	-0.45022275037584336
2316	5	-0.36160982313175793
2316	6	-0.4430708454773864
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2316	9	-0.7335990662892016
2316	10-11	-0.7723745271595348
2316	12-13	-0.5733223835241645
2316	14-15	-0.7152341985023369
2316	16-17	-0.832653537850824
2316	18-19	-0.6301915331609891
2316	20-21	-0.6238073868621328
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2316	54-55	-0.8085753860006477
2316	56-57	-0.7651154778321043
2316	58-59	-0.9097731147386625
2316	60-61	-0.7282770089738548
2316	62-63	-0.8767538899240606
2316	64-65	-0.7754225143502111
2316	66-67	-0.7078732014936904
2316	68-69	-0.6495604044666763
2316	70-71	-0.6319625842216539
2316	72-73	-0.6714785157733587
2316	74-75	-0.594511050495889
2316	76-77	-0.7888675159798169
2316	78-79	-1.0498885999780612
2316	80-81	-0.6026238571648364
2316	82-83	-0.3524798885350542
2316	84-85	-0.4203256488969558
2316	86-87	-0.3665574518180037
2316	88-89	-0.43988751976489127
2316	90-91	-0.33638547360269655
2316	92-93	-0.24883472053126354
2316	94-95	-0.2882470152867107
2316	96-97	-0.24638725246720838
2316	98-99	-0.1889121682162127
2316	100	-0.4292392410173278
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	3.0
1	18.0
2	19.0
3	23.0
4	37.0
5	29.0
6	43.0
7	267.0
8	638.0
9	943.0
10	1459.0
11	2191.0
12	3160.0
13	3726.0
14	4106.0
15	4614.0
16	5333.0
17	6208.0
18	7489.0
19	8850.0
20	10562.0
21	12515.0
22	14449.0
23	15504.0
24	15394.0
25	15619.0
26	16833.0
27	18203.0
28	21477.0
29	25274.0
30	30203.0
31	37112.0
32	47011.0
33	60547.0
34	84269.0
35	120800.0
36	183856.0
37	292693.0
38	305658.0
39	75573.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.29044938745593	18.966077187379117	13.55374852699131	23.18972489817364
2	15.833280504510663	20.577469563707425	40.14441562593886	23.444834305843052
3	18.119534531432897	26.556772016800938	30.763616332713628	24.560077119052536
4	12.466664509141138	17.21237143500466	38.58433732865747	31.736626727196732
5	12.906699569391103	37.70044933701102	35.16251457969845	14.230336513899427
6	29.593980469981513	37.35904202960204	18.726363597565424	14.320613902851031
7	25.9925876873557	33.10429140411284	23.294817025740716	17.60830388279074
8	25.42611944767788	35.09099933021409	21.114570562599514	18.368310659508516
9	26.4868217438473	15.504689212333483	20.955106918446806	37.05338212537241
10-11	24.333385764601047	26.134761207699754	29.25888460451623	20.27296842318297
12-13	25.017588423994603	24.251855217325456	28.996427243814793	21.734129114865144
14-15	22.62454466782007	25.438877915554514	26.86440928846666	25.072168128158758
16-17	21.910410341329833	28.14674356945362	27.51490114834443	22.42794494087212
18-19	21.728885905578217	27.23935904717647	29.489991566410218	21.541763480835098
20-21	22.59677412692271	26.499374185029183	29.42073905374573	21.48311263430237
22-23	22.470803220056105	26.38792523723621	29.209485798538857	21.93178574416882
24-25	22.027877306256233	26.542199941000042	29.310516345982062	22.11940640676166
26-27	21.902210222584934	26.565971597161436	29.2996561768894	22.232162003364227
28-29	21.93477201879001	26.72594918297194	29.34061110566024	21.998667692577808
30-31	22.390940371235768	26.55519175190733	29.25261298375138	21.801254893105522
32-33	22.102265725098814	26.692202460496627	29.1783106168729	22.027221197531656
34-35	22.26175268069866	26.811840410777116	29.121394538187285	21.80501237033694
36-37	22.27760332291194	26.710940947812023	29.002706440999106	22.00874928827693
38-39	22.237514143975776	26.849371090074964	28.90620723889283	22.006907527056434
40-41	22.232603581890395	26.69232323698292	29.07198792059568	22.00308526053101
42-43	22.127763887521283	26.849035673437964	28.804991127603447	22.218209311437302
44-45	22.100083045611736	26.993197972199688	28.778609764125672	22.128109218062903
46-47	22.12815967927549	26.860987365241694	28.50220126316601	22.508651692316803
48-49	22.153700630635004	26.92988045093449	28.487174747613476	22.429244170817032
50-51	22.160956113861566	27.22684366563552	28.344193830530806	22.268006389972108
52-53	22.29327166203707	27.356126605626496	28.05209746552591	22.298504266810518
54-55	22.22172878012507	27.209189039388725	28.22125025604481	22.34783192444139
56-57	22.199747120683607	27.347455698786515	28.0220300363897	22.430767144140177
58-59	22.206546731952905	27.266264497389965	28.196190580453305	22.33099819020383
60-61	22.359915944747165	27.302189925763127	28.22570983586917	22.112184293620533
62-63	22.245534725816377	27.272013528827788	28.28850066855577	22.193951076800065
64-65	22.18463017296168	27.084729120346047	28.20310310565336	22.52753760103891
66-67	22.122579285958206	27.140328434990472	28.15656704744489	22.58052523160643
68-69	21.974586103259877	27.204299224363186	28.176788732782278	22.64432593959466
70-71	22.117859078422825	27.029503333353993	28.156046830163184	22.696590758059994
72-73	22.081395585049922	27.437413900967478	27.84810693168441	22.633083582298195
74-75	22.082014039717222	27.340331614980656	27.937921697657153	22.639732647644966
76-77	22.068821407830473	27.264146104435948	27.947857416534443	22.71917507119913
78-79	22.079302566460967	27.17890322751649	27.99255503603895	22.74923916998359
80-81	22.102492498819228	27.374490339690922	27.741321832670312	22.78169532881954
82-83	22.243132335775194	27.338988587713644	27.787624693333314	22.63025438317785
84-85	22.22967688456465	27.439544945073408	27.655623056777216	22.675155113584726
86-87	21.987658889644308	27.462843274783346	27.833661798497378	22.71583603707497
88-89	21.99633947402572	27.226650722002088	27.909014897177226	22.867994906794966
90-91	22.271666617321724	27.2371676187945	27.82183630653811	22.66932945734566
92-93	22.158078861792536	27.274648871617728	27.75675111832987	22.810521148259866
94-95	22.069993005105722	27.220156035712517	27.754790401022245	22.955060558159516
96-97	22.033285716744402	27.121559988296646	27.86906011737776	22.976094177581192
98-99	22.092606170761346	27.02935294097398	27.77792694639224	23.100113941872433
100	22.175739420858726	27.057948559033157	27.74458561443377	23.021726405674343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	558.0
1	417.0
2	577.5
3	817.5
4	559.0
5	426.5
6	602.5
7	1159.0
8	1922.5
9	2362.0
10	2051.0
11	1543.5
12	1492.0
13	1549.0
14	1680.0
15	1805.5
16	1880.5
17	2021.5
18	2211.5
19	2382.5
20	2605.5
21	3014.5
22	3568.5
23	4355.0
24	5410.0
25	6745.5
26	8515.5
27	10543.5
28	12771.0
29	15081.0
30	17702.0
31	20540.0
32	23248.5
33	26363.0
34	29513.0
35	32286.5
36	35674.5
37	38973.0
38	41656.0
39	43746.5
40	44733.0
41	45720.5
42	46833.0
43	48074.5
44	49625.0
45	50964.0
46	51988.5
47	52809.5
48	54153.0
49	55547.0
50	55499.0
51	53810.5
52	51520.0
53	49505.5
54	46872.5
55	43466.5
56	39640.0
57	35634.5
58	31490.5
59	27164.5
60	22782.5
61	18671.5
62	15300.5
63	12088.0
64	9082.5
65	6816.5
66	5201.5
67	3984.5
68	3074.0
69	2360.0
70	1829.0
71	1428.0
72	1099.0
73	874.0
74	726.0
75	574.5
76	430.0
77	320.5
78	246.0
79	171.0
80	134.0
81	96.0
82	74.0
83	64.0
84	46.0
85	37.0
86	34.0
87	27.5
88	16.5
89	16.5
90	17.5
91	11.0
92	7.5
93	10.5
94	8.0
95	6.0
96	5.5
97	2.0
98	0.0
99	0.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17339146547795883
2	0.10696718434011435
3	0.03317772384294409
4	0.03448555943011408
5	0.028910049821652524
6	0.0030286718860778835
7	0.004405340925204194
8	0.005437842704548928
9	0.005919676868243136
10-11	0.005954093594221294
12-13	0.008397681138670496
14-15	0.018653865480161513
16-17	0.0086385982205176
18-19	0.017690197152773094
20-21	0.018998032739943087
22-23	0.04787366583561745
24-25	0.016416778291581256
26-27	0.01001526725964391
28-29	0.02853146583589279
30-31	0.015694027046039942
32-33	0.02140720355841413
34-35	0.021716954092217553
36-37	0.022095538077977286
38-39	0.021235119928523345
40-41	0.027017129892853847
42-43	0.03613756227706566
44-45	0.039097400711187225
46-47	0.02206112135199913
48-49	0.013663440213328633
50-51	0.013319272953547057
52-53	0.024126124910688596
54-55	0.02749896405654806
56-57	0.021372786832435976
58-59	0.0281184651241549
60-61	0.02780871459035148
62-63	0.05310500818429744
64-65	0.03407255871837619
66-67	0.021889037722108342
68-69	0.029391883985346735
70-71	0.04412224270399826
72-73	0.04147215480368011
74-75	0.04240140640509037
76-77	0.023506623843081758
78-79	0.017655780426794936
80-81	0.023678707472972543
82-83	0.01848178185027072
84-85	0.020512368682982028
86-87	0.021992287900042814
88-89	0.017793447330707566
90-91	0.028978883273608837
92-93	0.02705154661883201
94-95	0.02023703487515677
96-97	0.014248524554957316
98-99	0.01975520071146256
100	0.04350274163639142
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1452782.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.88407308462645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.22045119509801	63.067704225139344
2	10.808445217511224	16.187608031985835
3	2.4769768581778093	5.564583482301464
4	0.9585805044435052	2.8712965020898213
5	0.469063101234938	1.756267777708932
6	0.29424230452502564	1.322043734198456
7	0.17627889985635908	0.9240337414084799
8	0.12354967927837972	0.7401522570131476
9	0.08851731360000355	0.5965683282789718
>10	0.36661679109505213	4.519411748034191
>50	0.012364663985542817	0.6125860456887587
>100	0.0042256074332015885	0.564489519431577
>500	2.947987547050788E-4	0.17557565113774826
>1k	1.9653250313671916E-4	0.3017752495037324
>5k	1.9653250313671916E-4	0.795903706079583
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5528	0.3805113224145123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5288	0.3639912939449966	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2887	0.19872217579788293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	3.441672597815777E-5	0.0
18-19	0.0	0.0	0.0	6.883345195631554E-5	0.0
20-21	0.0	0.0	0.0	6.883345195631554E-5	3.441672597815777E-5
22-23	0.0	0.0	0.0	1.3766690391263107E-4	6.883345195631554E-5
24-25	0.0	0.0	0.0	3.785839857597355E-4	6.883345195631554E-5
26-27	0.0	0.0	0.0	6.539177935849976E-4	6.883345195631554E-5
28-29	0.0	0.0	0.0	0.0013078355871699952	6.883345195631554E-5
30-31	0.0	0.0	0.0	0.0032351722419468303	6.883345195631554E-5
32-33	0.0	0.0	0.0	0.008087930604867075	6.883345195631554E-5
34-35	0.0	0.0	0.0	0.016107027757777836	6.883345195631554E-5
36-37	0.0	0.0	0.0	0.031663387899905145	6.883345195631554E-5
38-39	0.0	3.441672597815777E-5	0.0	0.05482584448320532	6.883345195631554E-5
40-41	0.0	6.883345195631554E-5	0.0	0.08545673060376574	1.032501779344733E-4
42-43	0.0	6.883345195631554E-5	0.0	0.11519278184889406	1.3766690391263107E-4
44-45	0.0	6.883345195631554E-5	0.0	0.15046992597650577	1.3766690391263107E-4
46-47	0.0	6.883345195631554E-5	0.0	0.19748317366266926	1.3766690391263107E-4
48-49	0.0	6.883345195631554E-5	0.0	0.25072584875087933	1.3766690391263107E-4
50-51	0.0	6.883345195631554E-5	0.0	0.3070660291771236	1.3766690391263107E-4
52-53	0.0	6.883345195631554E-5	0.0	0.36251137472793576	1.3766690391263107E-4
54-55	0.0	6.883345195631554E-5	0.0	0.4307597423426226	1.3766690391263107E-4
56-57	0.0	6.883345195631554E-5	0.0	0.5154593049748689	1.3766690391263107E-4
58-59	0.0	6.883345195631554E-5	0.0	0.6131339733008807	1.3766690391263107E-4
60-61	0.0	6.883345195631554E-5	0.0	0.7158679003456816	1.3766690391263107E-4
62-63	0.0	6.883345195631554E-5	0.0	0.8215616658246041	2.0650035586894662E-4
64-65	0.0	6.883345195631554E-5	0.0	0.9352745284564374	2.0650035586894662E-4
66-67	0.0	6.883345195631554E-5	0.0	1.0665743380631092	2.0650035586894662E-4
68-69	0.0	6.883345195631554E-5	0.0	1.2257172789861108	2.0650035586894662E-4
70-71	0.0	6.883345195631554E-5	0.0	1.3971469910833147	2.409170818471044E-4
72-73	0.0	6.883345195631554E-5	0.0	1.5725002099420284	2.7533380782526214E-4
74-75	0.0	6.883345195631554E-5	0.0	1.7518457690142086	2.7533380782526214E-4
76-77	0.0	6.883345195631554E-5	0.0	1.950636778264048	2.7533380782526214E-4
78-79	0.0	6.883345195631554E-5	0.0	2.1861160174066034	2.7533380782526214E-4
80-81	0.0	6.883345195631554E-5	0.0	2.436463282171723	3.097505338034199E-4
82-83	0.0	6.883345195631554E-5	0.0	2.7033994088583144	3.441672597815777E-4
84-85	0.0	6.883345195631554E-5	0.0	2.9771844640145595	3.441672597815777E-4
86-87	0.0	6.883345195631554E-5	0.0	3.262395872195553	4.1300071173789324E-4
88	0.0	6.883345195631554E-5	0.0	3.4926093522634503	4.818341636942088E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2945	0.0	55.80627	1
GTATCAA	5180	0.0	37.72778	1
TCAACGC	6145	0.0	30.967281	4
CAACGCA	6175	0.0	30.889734	5
TATCAAC	6275	0.0	30.799385	2
ATCAACG	6210	0.0	30.719868	3
AACGCAG	6385	0.0	29.8707	6
TACCTGG	1115	0.0	28.677944	2
ACGCAGA	7150	0.0	26.674744	7
CGCAGAG	7360	0.0	25.851595	8
GTACCTG	1350	0.0	25.115486	1
GTGGTAT	1425	0.0	23.463154	1
GCAGAGT	8030	0.0	23.287483	9
GTACATG	5240	0.0	22.647076	1
GAGTACT	5080	0.0	22.523848	12-13
GTACCGT	150	3.3048993E-5	21.922346	6
CAGAGTA	7700	0.0	21.84476	10-11
TACATGG	5345	0.0	21.466246	2
ACATGGG	5445	0.0	20.883455	3
CATGGGG	3375	0.0	20.743519	4
>>END_MODULE