FastQCFastQC Report
Mon 6 Jun 2016
ERR1211558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452782
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64310.4426679295310652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59630.4104538740155096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.2464237580036096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.052.8197631
GTATCAA51150.036.855261
ATCAACG65600.028.5808723
TCAACGC65550.028.3876134
CAACGCA66150.028.130135
TATCAAC66950.028.0863442
GTACCTG13000.027.8450581
AACGCAG68100.027.3246426
TACCTGG13000.027.1208762
GTACATG57750.024.584181
ACGCAGA76800.024.2292737
CGCAGAG77650.023.903538
TACATGG59600.023.662512
ACATGGG58850.022.5169243
CATGGGG36100.022.3886384
GTATCGC1050.00233864922.376246
GCAGAGT86400.021.2108149
ACCTGGG16000.021.1455483
GAGTACT51500.020.80338512-13
ATGGGGG22500.020.46685
Produced by FastQC (version 0.11.5)