##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211558_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1452782 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11310712825462 33.0 31.0 34.0 30.0 34.0 2 32.33475290855751 34.0 31.0 34.0 30.0 34.0 3 32.33878241883504 34.0 31.0 34.0 30.0 34.0 4 35.98280884537391 37.0 35.0 37.0 35.0 37.0 5 35.90809426328245 37.0 35.0 37.0 35.0 37.0 6 35.92496878402954 37.0 35.0 37.0 35.0 37.0 7 35.85371996624408 37.0 35.0 37.0 35.0 37.0 8 35.85417495536151 37.0 35.0 37.0 35.0 37.0 9 37.49932061382919 39.0 37.0 39.0 35.0 39.0 10-11 37.46681057447022 39.0 37.0 39.0 34.5 39.0 12-13 37.38354205930415 39.0 37.0 39.0 34.0 39.0 14-15 38.60173687449321 40.0 38.0 41.0 34.0 41.0 16-17 38.531238341334074 40.0 38.0 41.0 34.0 41.0 18-19 38.53093616247999 40.0 38.0 41.0 34.0 41.0 20-21 38.475249555680065 40.0 38.0 41.0 34.0 41.0 22-23 38.36262047574928 40.0 38.0 41.0 34.0 41.0 24-25 38.25105074264411 40.0 38.0 41.0 33.5 41.0 26-27 38.069861823728544 40.0 37.0 41.0 33.0 41.0 28-29 38.09703623805912 40.0 38.0 41.0 33.0 41.0 30-31 38.008088963106644 40.0 37.0 41.0 33.0 41.0 32-33 37.809856881486695 40.0 37.0 41.0 33.0 41.0 34-35 37.68193885937463 40.0 37.0 41.0 33.0 41.0 36-37 37.62068293797693 40.0 37.0 41.0 33.0 41.0 38-39 37.51140122881478 40.0 36.5 41.0 32.0 41.0 40-41 37.34246191100936 40.0 36.0 41.0 31.5 41.0 42-43 37.17998984018249 40.0 36.0 41.0 31.0 41.0 44-45 36.985517097541134 39.0 36.0 41.0 30.5 41.0 46-47 36.70853989105041 39.0 35.0 41.0 30.0 41.0 48-49 36.46046000019273 39.0 35.0 41.0 29.5 41.0 50-51 36.24273084330615 39.0 35.0 40.5 28.5 41.0 52-53 36.02994736994263 39.0 35.0 40.0 28.0 41.0 54-55 35.83250033384224 38.5 35.0 40.0 28.0 41.0 56-57 35.62975759611559 38.0 34.0 40.0 27.0 41.0 58-59 35.36822248623675 38.0 34.0 40.0 26.5 41.0 60-61 35.049746624063346 38.0 34.0 40.0 26.0 41.0 62-63 34.72941914203232 37.0 33.5 40.0 26.0 41.0 64-65 34.41149222663827 37.0 33.0 39.5 26.0 41.0 66-67 34.06553770627664 36.0 33.0 39.0 25.0 40.5 68-69 33.67455888082314 36.0 32.0 39.0 24.5 40.0 70-71 33.27939601399247 35.0 32.0 38.5 24.0 40.0 72-73 32.86519002851082 35.0 31.0 37.5 23.0 40.0 74-75 32.46352171213575 35.0 31.0 37.0 22.0 39.0 76-77 30.92854089601881 33.5 29.5 35.5 20.5 38.0 78-79 31.761161000067457 34.0 30.5 36.0 22.0 38.5 80-81 31.73824221390408 34.0 31.0 36.0 21.5 37.5 82-83 31.459818816587763 34.0 30.5 35.0 20.5 37.0 84-85 31.164905677520785 34.0 30.0 35.0 20.0 37.0 86-87 30.871906452585453 34.0 30.0 35.0 20.0 36.0 88-89 30.601361043845532 34.0 29.5 35.0 19.5 36.0 90-91 30.317727986717898 34.0 29.0 35.0 18.5 35.5 92-93 30.105015067642633 34.0 29.0 35.0 18.0 35.0 94-95 29.88138034474546 34.0 29.0 35.0 18.0 35.0 96-97 29.678000897588213 34.0 29.0 35.0 17.5 35.0 98-99 29.520299673316437 33.5 29.0 35.0 17.0 35.0 100 27.625532254667252 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2750819602146066 1101 2 0.21219465762630563 1101 3 0.2961138295599284 1101 4 0.14849057338486205 1101 5 0.20525880829251264 1101 6 0.21414501369041972 1101 7 0.27019337861698745 1101 8 0.218345378699496 1101 9 0.2758788161540906 1101 10-11 0.2862409359052265 1101 12-13 0.29164387534850533 1101 14-15 0.29542382134363265 1101 16-17 0.2981806078244489 1101 18-19 0.34759294330127943 1101 20-21 0.358669036730781 1101 22-23 0.32854627832084304 1101 24-25 0.39862688222279274 1101 26-27 0.274799870227767 1101 28-29 0.3600875301637245 1101 30-31 0.390862891969725 1101 32-33 0.401696065433498 1101 34-35 0.43561311821527937 1101 36-37 0.36822753557935073 1101 38-39 0.41456630683208573 1101 40-41 0.3753213574830241 1101 42-43 0.42560363105307175 1101 44-45 0.4185041691059723 1101 46-47 0.43401503192753665 1101 48-49 0.4488094177984294 1101 50-51 0.39892202319403935 1101 52-53 0.4295955223575092 1101 54-55 0.46060663263725843 1101 56-57 0.2364866704571682 1101 58-59 0.31095743805617815 1101 60-61 0.17067542909523326 1101 62-63 0.16114622203059525 1101 64-65 0.18499830297257347 1101 66-67 0.13660446961835504 1101 68-69 0.12757256639444137 1101 70-71 0.10768777666134355 1101 72-73 0.09848033281447499 1101 74-75 0.059265024641383945 1101 76-77 0.3000709195456963 1101 78-79 0.3433656990593903 1101 80-81 0.3221484533233969 1101 82-83 0.3266097246508224 1101 84-85 0.34640648115837536 1101 86-87 0.23543406341591933 1101 88-89 0.03884695667112936 1101 90-91 0.19085861717965358 1101 92-93 0.20001165291079914 1101 94-95 0.25971938399645467 1101 96-97 0.19234847728734294 1101 98-99 0.025444818787146772 1101 100 0.2761803420725286 1102 1 0.36814873678311244 1102 2 0.30189233049959086 1102 3 0.395768370439761 1102 4 0.22370502010150517 1102 5 0.22975564525476955 1102 6 0.25405834653098225 1102 7 0.23148508364103293 1102 8 0.27209984812819243 1102 9 0.41239415126884893 1102 10-11 0.3255114325615054 1102 12-13 0.3149868176268953 1102 14-15 0.427397679338803 1102 16-17 0.38347791396006414 1102 18-19 0.36285694290707227 1102 20-21 0.40572038971055946 1102 22-23 0.43510906833441254 1102 24-25 0.3315221760795666 1102 26-27 0.4197458040462436 1102 28-29 0.5535358647252409 1102 30-31 0.603192392924278 1102 32-33 0.6213009638302935 1102 34-35 0.4984673407381379 1102 36-37 0.6526808672402638 1102 38-39 0.6185837343375482 1102 40-41 0.5644003098616324 1102 42-43 0.5514399340506557 1102 44-45 0.6632590066162507 1102 46-47 0.611772302658629 1102 48-49 0.6751639094762822 1102 50-51 0.677403548559937 1102 52-53 0.6405137328389543 1102 54-55 0.5480840568426899 1102 56-57 0.583953813763344 1102 58-59 0.5370601335631164 1102 60-61 0.6299635906808021 1102 62-63 0.67715217106511 1102 64-65 0.7522049221694047 1102 66-67 0.7829510546351202 1102 68-69 0.689402805378684 1102 70-71 0.5111049997434165 1102 72-73 0.659759569355046 1102 74-75 0.6855519102605854 1102 76-77 0.8125909195158272 1102 78-79 0.875820900246211 1102 80-81 0.7236420208551699 1102 82-83 0.645181530796517 1102 84-85 0.6200833473431757 1102 86-87 0.5720614655588534 1102 88-89 0.5734664103425011 1102 90-91 0.6176001171456846 1102 92-93 0.5685361609470014 1102 94-95 0.41473212499469625 1102 96-97 0.43003734308091524 1102 98-99 0.5158330739236767 1102 100 0.5312463167756931 1103 1 0.26697047620137937 1103 2 0.18164863705305123 1103 3 0.24108160269170043 1103 4 0.12474242649948764 1103 5 0.12587699378958206 1103 6 0.0893528293930359 1103 7 0.14536056719614265 1103 8 0.08645021898311711 1103 9 0.22174954702011007 1103 10-11 0.17585704145289327 1103 12-13 0.22376073170135413 1103 14-15 0.27155239901990313 1103 16-17 0.23752889300136104 1103 18-19 0.1654155445683756 1103 20-21 0.22947836886070405 1103 22-23 0.2875282183695447 1103 24-25 0.1892175607674531 1103 26-27 0.3045152254482275 1103 28-29 0.4032910903743172 1103 30-31 0.4061719488707993 1103 32-33 0.45265781300523145 1103 34-35 0.4829026916225203 1103 36-37 0.45412303305847956 1103 38-39 0.43968601743949876 1103 40-41 0.505861483140329 1103 42-43 0.4694026574359995 1103 44-45 0.47295606539687896 1103 46-47 0.483541885261225 1103 48-49 0.43286367014385263 1103 50-51 0.6281870546322637 1103 52-53 0.5950168216447835 1103 54-55 0.8193530946919267 1103 56-57 0.8780323652168249 1103 58-59 0.8125357221417318 1103 60-61 0.7575927783905101 1103 62-63 0.7089257107501936 1103 64-65 0.3895040899478346 1103 66-67 0.4967199064117054 1103 68-69 0.4474561975670781 1103 70-71 0.6150406885506712 1103 72-73 0.46793980885203723 1103 74-75 0.48063899412642996 1103 76-77 0.5487818049859712 1103 78-79 0.774073949312907 1103 80-81 0.5585168584857492 1103 82-83 0.682164169377625 1103 84-85 0.5435053858989214 1103 86-87 0.4166023560969023 1103 88-89 0.5883785896664087 1103 90-91 0.7448466871443387 1103 92-93 0.6181079694645994 1103 94-95 0.6671002967365602 1103 96-97 0.8689434304426769 1103 98-99 0.6698597045651873 1103 100 0.48193800207767623 1104 1 0.2612687514646126 1104 2 0.2602008123410684 1104 3 0.318180374311126 1104 4 0.04306471458654926 1104 5 0.19018223886351393 1104 6 0.18090210875190849 1104 7 0.18458731448572507 1104 8 0.1465129705897894 1104 9 0.2661341096275862 1104 10-11 0.33534060722980286 1104 12-13 0.2835177285132673 1104 14-15 0.3580598323191282 1104 16-17 0.3214414898658049 1104 18-19 0.389202801392301 1104 20-21 0.3117959513542772 1104 22-23 0.3192727145889904 1104 24-25 0.2874744087812857 1104 26-27 0.3348498307525887 1104 28-29 0.45446034022221227 1104 30-31 0.20884782780899513 1104 32-33 0.2879408673518853 1104 34-35 0.47356396115372235 1104 36-37 0.367222430950747 1104 38-39 0.47738301948848516 1104 40-41 0.5173261224234906 1104 42-43 0.4360885940363701 1104 44-45 0.4406661057316441 1104 46-47 0.5134450538444 1104 48-49 0.3588513902232009 1104 50-51 0.44431731005989406 1104 52-53 0.4325728346022544 1104 54-55 0.43511955241967826 1104 56-57 0.5859796199894305 1104 58-59 0.6232909869906536 1104 60-61 0.524999628074923 1104 62-63 0.5066982462771961 1104 64-65 0.49182355281397605 1104 66-67 0.6267624323581273 1104 68-69 0.4488043159124473 1104 70-71 0.4713437051500051 1104 72-73 0.6153573269312034 1104 74-75 0.8175881595751235 1104 76-77 0.5519419187096588 1104 78-79 0.5176284284458177 1104 80-81 0.6845208569970005 1104 82-83 0.4559234925678197 1104 84-85 0.47795575377594446 1104 86-87 0.6626426955274596 1104 88-89 0.7747340002830541 1104 90-91 0.4940516081259574 1104 92-93 0.4298479046150554 1104 94-95 0.5822164330526967 1104 96-97 0.5512061169356208 1104 98-99 0.8231384293928627 1104 100 1.0097647964542737 1105 1 0.21208638118786638 1105 2 0.15658530104784063 1105 3 0.21569407320502165 1105 4 0.1516648337369091 1105 5 0.08134653724683005 1105 6 0.14937709736919658 1105 7 0.13048489500849314 1105 8 0.1489718625820231 1105 9 0.2100971956672808 1105 10-11 0.173026553150045 1105 12-13 0.17239642393005994 1105 14-15 0.2120196564477581 1105 16-17 0.20937389128926753 1105 18-19 0.2027460749465817 1105 20-21 0.2885604980052676 1105 22-23 0.24926621341900557 1105 24-25 0.18669381533999996 1105 26-27 0.1229286783918937 1105 28-29 0.3432421976540496 1105 30-31 0.34132898687472846 1105 32-33 0.292955489321308 1105 34-35 0.2516650985186786 1105 36-37 0.2364860545262104 1105 38-39 0.3767366400965173 1105 40-41 0.41445869872162433 1105 42-43 0.32444628976801937 1105 44-45 0.38525552400151497 1105 46-47 0.48726991640241835 1105 48-49 0.42546403148773493 1105 50-51 0.43108052023342935 1105 52-53 0.4734747077093857 1105 54-55 0.43216118029452133 1105 56-57 0.4511635895086812 1105 58-59 0.5165166319542891 1105 60-61 0.5077171624325914 1105 62-63 0.3390054367524158 1105 64-65 0.5116804364656886 1105 66-67 0.47327365990134496 1105 68-69 0.5492711769346457 1105 70-71 0.5525403734702792 1105 72-73 0.4966311258570357 1105 74-75 0.43127142347957914 1105 76-77 0.5157448992809037 1105 78-79 0.4833534448120105 1105 80-81 0.49099212612958 1105 82-83 0.5423380859955209 1105 84-85 0.5403239465186438 1105 86-87 0.3746923988974231 1105 88-89 0.3891500615707386 1105 90-91 0.5920425880357527 1105 92-93 0.4921339832769469 1105 94-95 0.49390981794234534 1105 96-97 0.6241794847908437 1105 98-99 0.7129469959868153 1105 100 0.9220329799095879 1106 1 0.2123907286155955 1106 2 0.23405367221944573 1106 3 0.22035767966627162 1106 4 0.1420663373088118 1106 5 0.22607568994597926 1106 6 0.14069243994529046 1106 7 0.20260408128545038 1106 8 0.13537940370577672 1106 9 0.1601750552284713 1106 10-11 0.194050427293611 1106 12-13 0.19275662946814265 1106 14-15 0.2958606006577682 1106 16-17 0.22019555365563548 1106 18-19 0.33214691285807163 1106 20-21 0.285254105793058 1106 22-23 0.10792730781889048 1106 24-25 0.3736922047684672 1106 26-27 0.3504592535567497 1106 28-29 0.25844478986554975 1106 30-31 0.32954787322606194 1106 32-33 0.3595719227279801 1106 34-35 0.2963470056616515 1106 36-37 0.253100077851812 1106 38-39 0.30552511823588446 1106 40-41 0.440003566264771 1106 42-43 0.5450117306395725 1106 44-45 0.6128327073457456 1106 46-47 0.5433694332309571 1106 48-49 0.5016116383967386 1106 50-51 0.5231258982755662 1106 52-53 0.4133260555024094 1106 54-55 0.3093637653355259 1106 56-57 0.48243249918402853 1106 58-59 0.6603905046438427 1106 60-61 0.6193142733628392 1106 62-63 0.4479885060324875 1106 64-65 0.5114031775524666 1106 66-67 0.5664818522648503 1106 68-69 0.45861528034266286 1106 70-71 0.5156013995115032 1106 72-73 0.6830457179617611 1106 74-75 0.663758798439197 1106 76-77 0.5892597415958676 1106 78-79 0.7071991852825263 1106 80-81 0.6612579799814604 1106 82-83 0.7231965449219651 1106 84-85 0.7280505389548146 1106 86-87 0.3784198581660583 1106 88-89 0.5541802632726132 1106 90-91 0.4614275472974185 1106 92-93 0.7527027863111826 1106 94-95 0.8062864008776174 1106 96-97 0.9053576678606028 1106 98-99 0.6011303884169301 1106 100 0.6705448046437148 1107 1 0.2646812192176071 1107 2 0.2319128619520896 1107 3 0.23891533387311625 1107 4 0.19055267492759498 1107 5 0.1492862736459628 1107 6 0.2644644171942119 1107 7 0.19972532932984421 1107 8 0.22800400673908428 1107 9 0.21524954262768148 1107 10-11 0.21197822632407792 1107 12-13 0.20328819360097583 1107 14-15 0.22248790769975102 1107 16-17 0.3748669419458608 1107 18-19 0.24423278974528273 1107 20-21 0.2820707836854126 1107 22-23 0.40772319454258366 1107 24-25 0.3579653096447828 1107 26-27 0.3398351903407004 1107 28-29 0.366000918965554 1107 30-31 0.3552406729005355 1107 32-33 0.3425283000782997 1107 34-35 0.2703028735491628 1107 36-37 0.2939313472181695 1107 38-39 0.2677180151266896 1107 40-41 0.17706600047966248 1107 42-43 0.26438058744934523 1107 44-45 0.3559861344060167 1107 46-47 0.2681077490174957 1107 48-49 0.46946502796514267 1107 50-51 0.21470503606104785 1107 52-53 0.24174210202185975 1107 54-55 0.3567470872790963 1107 56-57 0.31197494252167246 1107 58-59 0.5983560252516611 1107 60-61 0.44361277754396866 1107 62-63 0.3305604071491217 1107 64-65 0.566673533298605 1107 66-67 0.6531975892507873 1107 68-69 0.46143332336560405 1107 70-71 0.5068783872983502 1107 72-73 0.6151085254545592 1107 74-75 0.6569557062215168 1107 76-77 0.5272806385949593 1107 78-79 0.6087986195409059 1107 80-81 0.7097769281417818 1107 82-83 0.7053224815008186 1107 84-85 0.6479299250726491 1107 86-87 0.5662721648183613 1107 88-89 0.5470927004819401 1107 90-91 0.32293878132133 1107 92-93 0.40205250339935006 1107 94-95 0.5754665267420016 1107 96-97 0.6878603279783597 1107 98-99 0.6598601278361436 1107 100 0.911211421539992 1108 1 0.30418416158317996 1108 2 0.2023504305377628 1108 3 0.27217978740170423 1108 4 0.16394464165993128 1108 5 0.18947747927784775 1108 6 0.24429300915837615 1108 7 0.22723580569144985 1108 8 0.16104834436992377 1108 9 0.13039101361794536 1108 10-11 0.2079039965692786 1108 12-13 0.21442514355188536 1108 14-15 0.2472248756193025 1108 16-17 0.36626084351619426 1108 18-19 0.3331326594726107 1108 20-21 0.37313852226729693 1108 22-23 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46-47 -0.5691777730282013 2316 48-49 -0.5938735783924614 2316 50-51 -0.6182595657088612 2316 52-53 -0.7279849823161157 2316 54-55 -0.5428400854241247 2316 56-57 -0.7235063743550469 2316 58-59 -0.7230961688912174 2316 60-61 -0.5933783566069835 2316 62-63 -0.6298572913142664 2316 64-65 -0.6090501363352914 2316 66-67 -0.4021341388240742 2316 68-69 -0.416611970338046 2316 70-71 -0.4906265653258899 2316 72-73 -0.4016167635571293 2316 74-75 -0.6663170660750737 2316 76-77 -0.43379564485154276 2316 78-79 -0.3312306400417704 2316 80-81 -0.40856024456143913 2316 82-83 -0.4227134445747467 2316 84-85 -0.528538267349834 2316 86-87 -0.20865102063135055 2316 88-89 -0.25971585452477086 2316 90-91 -0.09259372353354678 2316 92-93 -0.0441292513936169 2316 94-95 -0.05094309764055183 2316 96-97 -0.11635135760819892 2316 98-99 -0.10552640493589394 2316 100 -0.425647669783789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 4.0 11 4.0 12 14.0 13 34.0 14 157.0 15 398.0 16 955.0 17 1953.0 18 3395.0 19 5221.0 20 7410.0 21 10036.0 22 13345.0 23 15510.0 24 16026.0 25 16904.0 26 19202.0 27 22812.0 28 27139.0 29 32582.0 30 39455.0 31 47497.0 32 57924.0 33 73310.0 34 97050.0 35 132047.0 36 193548.0 37 293636.0 38 282624.0 39 42589.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88065311067362 20.477373790217108 14.668075498712776 22.97389760039649 2 15.247214995526221 21.560236204472677 39.74030733031499 23.452241469686104 3 16.985540047261406 27.486437986441082 31.46827683096648 24.05974513533103 4 12.228699499095178 18.268880642578907 38.34210622321068 31.160313635115237 5 12.602529261624113 38.14418938899581 35.29904540060409 13.954235948775981 6 29.519809303148385 38.08767622284769 18.421684814668733 13.970829659335191 7 25.908773649453252 33.543435973188 23.1261813541192 17.42160902323955 8 25.593350073790173 35.637761844974555 20.737653913081786 18.031234168153485 9 26.425575206741275 15.48181351365862 20.944573927815735 37.14803735178437 10-11 24.40238797011527 26.60440451492378 28.9934415486976 19.99976596626335 12-13 25.124347630959083 24.558089238440452 28.908225735175684 21.409337395424778 14-15 22.655394959463983 25.6937723622677 26.834927745525484 24.815904932742832 16-17 21.823571501584382 28.53851128409035 27.473412099804822 22.16450511452045 18-19 21.655874047292496 27.53857723276436 29.62504323644355 21.180505483499594 20-21 22.685232987641267 26.653257504106037 29.595185091874793 21.066324416377903 22-23 22.417162382243173 26.533161892148993 29.3903696494037 21.65930607620414 24-25 22.082019904576153 26.705943047870583 29.423006832066623 21.789030215486637 26-27 22.03067362620192 26.764153391534435 29.415416935404494 21.789756046859157 28-29 22.04349832750366 26.863364672348006 29.459545688112044 21.633591312036295 30-31 22.445719792464356 26.651637827931268 29.43364796379312 21.468994415811256 32-33 22.205246742554976 26.805903841613382 29.35121621147218 21.63763320435946 34-35 22.352387249234802 26.929670616360866 29.305097270412194 21.412844863992138 36-37 22.337809839134895 26.84048404083234 29.189100821189868 21.6326052988429 38-39 22.356583331038852 26.96625733087365 29.04560973595088 21.631549602136623 40-41 22.32638054715933 26.880855894150425 29.129457294436644 21.663306264253603 42-43 22.19887841897897 27.020513443586914 28.93569581494636 21.844912322487758 44-45 22.125754541706048 27.15706513994946 28.845159678199877 21.872020640144612 46-47 22.18594823286777 27.017333842278248 28.650902453639947 22.145815471214036 48-49 22.19739400047362 27.087291621920794 28.600356039453466 22.114958338152118 50-51 22.250760252436507 27.39841358076746 28.345583996062345 22.00524217073368 52-53 22.37269794854639 27.417076585674106 28.12595079870533 22.084274667074176 54-55 22.233537898893378 27.371436379261866 28.2469806474395 22.14804507440526 56-57 22.331104219942247 27.483384902374468 28.00660129616691 22.178909581516372 58-59 22.27623181392493 27.340815591107887 28.260603947545494 22.122348647421692 60-61 22.457447438310314 27.45054981365399 28.23472437725188 21.85727837078381 62-63 22.239865341654248 27.523656428455865 28.21601787183362 22.020460358056265 64-65 22.20800761049623 27.239704817927347 28.27479012796936 22.277497443607057 66-67 22.179861966605845 27.26402199612493 28.131041886743226 22.425074150525997 68-69 22.01351383912129 27.38118595469456 28.203203254886976 22.40209695129717 70-71 22.097852660331128 27.26270208428486 28.12874194304485 22.51070331233916 72-73 22.09906110105675 27.603690886849563 27.796957166134657 22.500290845959032 74-75 22.10325932263883 27.577946646355628 27.794537290039067 22.524256740966468 76-77 21.987691496490587 27.480329351075955 27.938912486133916 22.593066666299542 78-79 21.956405910754924 27.380437730695256 27.99093258363542 22.6722237749144 80-81 21.94624138886451 27.64047573565729 27.694857325768098 22.71842554971011 82-83 22.02946652718585 27.5973396753732 27.74298215630044 22.63021164114051 84-85 22.03260364067218 27.66025487665632 27.63701918029283 22.67012230237868 86-87 21.863123368254072 27.731694151602277 27.64088095002038 22.76430153012327 88-89 21.759417199593372 27.524251507209783 27.755896905576876 22.960434387619966 90-91 21.987087641411808 27.565103996331608 27.64087476857382 22.806933593682764 92-93 21.891206739421367 27.55242535367123 27.610646098779707 22.945721808127697 94-95 21.669469947509498 27.53842101548203 27.606181793766904 23.18592724324157 96-97 21.640891365055932 27.517722097679464 27.63469895843087 23.20668757883373 98-99 21.612542224569133 27.4132247023497 27.562587715055713 23.41164535802545 100 20.78362524785195 28.00320830579423 27.721483256223838 23.491683190129983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 599.0 1 438.0 2 544.5 3 775.5 4 564.5 5 444.5 6 648.0 7 1232.0 8 2011.0 9 2495.5 10 2201.5 11 1699.0 12 1713.5 13 1852.5 14 1978.0 15 2134.0 16 2248.5 17 2374.0 18 2591.5 19 2722.5 20 2856.5 21 3234.0 22 3835.0 23 4672.5 24 5693.5 25 7067.0 26 8762.5 27 10765.5 28 13003.0 29 15404.0 30 18130.5 31 20784.0 32 23745.0 33 26862.5 34 29802.5 35 32575.5 36 36096.5 37 39419.5 38 41796.0 39 44216.5 40 45369.5 41 46209.5 42 47298.0 43 48396.5 44 49831.5 45 51169.0 46 52098.5 47 52832.5 48 54137.0 49 55063.0 50 55123.5 51 53647.5 52 51064.5 53 48640.0 54 46104.5 55 43085.0 56 39128.0 57 34926.0 58 30846.0 59 26614.0 60 22059.5 61 17989.5 62 14702.0 63 11431.5 64 8510.0 65 6410.0 66 4866.5 67 3698.5 68 2839.5 69 2198.0 70 1688.5 71 1240.0 72 975.0 73 773.5 74 609.0 75 465.5 76 309.0 77 219.0 78 164.5 79 105.5 80 68.5 81 59.0 82 39.5 83 21.5 84 17.0 85 14.5 86 8.5 87 3.5 88 4.0 89 6.5 90 4.5 91 2.0 92 1.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0028910049821652527 2 0.06807628398479607 3 7.571679715194709E-4 4 7.571679715194709E-4 5 0.0017896697508642042 6 3.441672597815777E-4 7 0.0 8 9.636683273884176E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.4741743771605104E-4 18-19 9.980850533665752E-4 20-21 0.0030286718860778835 22-23 0.0 24-25 3.441672597815777E-5 26-27 2.0650035586894662E-4 28-29 9.292516014102597E-4 30-31 0.001342252313148153 32-33 0.0022715039145584127 34-35 0.0036825896796628815 36-37 8.260014234757865E-4 38-39 0.0015143359430389418 40-41 0.005437842704548928 42-43 0.006917761921609712 44-45 0.005437842704548928 46-47 0.007124262277478658 48-49 0.009223682562146281 50-51 0.009602266547906017 52-53 0.0038546733095536703 54-55 0.0019617533807549927 56-57 0.0025468377223836747 58-59 0.003889090035531828 60-61 0.007434012811282077 62-63 0.014936859074520472 64-65 0.00354492277575025 66-67 0.011185435942901275 68-69 0.013560190035394161 70-71 0.005334592526614454 72-73 0.008466514590626811 74-75 0.0028565882561870947 76-77 0.0032007555159686723 78-79 0.007434012811282077 80-81 0.005816426690308663 82-83 0.017380446618969675 84-85 0.018963616013964932 86-87 0.024091708184710438 88-89 0.023850791102863332 90-91 0.025812544483618326 92-93 0.0378928153019517 94-95 0.042298156227155895 96-97 0.024642375800360966 98-99 0.019307783273746507 100 0.020787702490807292 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1452782.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.95860581640228 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80495695737254 61.980977770708755 2 10.975375194138119 16.234468953407617 3 2.627654578745766 5.830130076333681 4 0.9969076267157931 2.949195927985538 5 0.48775412618953845 1.8036807577087908 6 0.2809090768164424 1.2465386209510039 7 0.20135256429160892 1.0424228452795432 8 0.12530649819620418 0.741399512506143 9 0.08673304033351947 0.5773189267156398 >10 0.3945286778440497 4.8100020274097925 >50 0.013263989696151146 0.6873556476143408 >100 0.004364885995325457 0.5710181244690458 >500 4.959909249843789E-4 0.27071904414361136 >1k 1.983963699937516E-4 0.3454785850602347 >5k 1.983963699937516E-4 0.9092931797062113 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6431 0.4426679295310652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5963 0.4104538740155096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3580 0.2464237580036096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.441672597815777E-5 6.883345195631554E-5 18-19 0.0 0.0 0.0 6.883345195631554E-5 1.3766690391263107E-4 20-21 0.0 0.0 0.0 6.883345195631554E-5 1.3766690391263107E-4 22-23 0.0 0.0 0.0 1.3766690391263107E-4 1.3766690391263107E-4 24-25 0.0 0.0 0.0 3.785839857597355E-4 1.3766690391263107E-4 26-27 0.0 0.0 0.0 6.539177935849976E-4 1.3766690391263107E-4 28-29 0.0 0.0 0.0 0.0013078355871699952 1.7208362989078885E-4 30-31 0.0 0.0 0.0 0.0032007555159686727 2.0650035586894662E-4 32-33 0.0 0.0 0.0 0.008053513878888918 2.0650035586894662E-4 34-35 0.0 0.0 0.0 0.01621027793571231 2.0650035586894662E-4 36-37 0.0 0.0 0.0 0.03159455444794883 2.0650035586894662E-4 38-39 0.0 0.0 0.0 0.05472259430527085 2.0650035586894662E-4 40-41 0.0 0.0 0.0 0.08566323095963468 2.0650035586894662E-4 42-43 0.0 0.0 0.0 0.11550253238269748 2.0650035586894662E-4 44-45 0.0 0.0 0.0 0.1506764263323747 2.0650035586894662E-4 46-47 0.0 0.0 0.0 0.1981026747302761 2.0650035586894662E-4 48-49 0.0 0.0 0.0 0.2514830167223988 2.0650035586894662E-4 50-51 0.0 0.0 0.0 0.307375779710927 2.0650035586894662E-4 52-53 0.0 0.0 0.0 0.36278670853576106 2.7533380782526214E-4 54-55 0.0 0.0 0.0 0.4304499918088192 3.441672597815777E-4 56-57 0.0 0.0 0.0 0.5139105523058518 3.441672597815777E-4 58-59 0.0 0.0 0.0 0.6102429683187154 3.441672597815777E-4 60-61 0.0 0.0 0.0 0.7135275629791669 3.441672597815777E-4 62-63 0.0 0.0 0.0 0.8177758259670067 3.441672597815777E-4 64-65 0.0 0.0 0.0 0.9301120195597137 3.441672597815777E-4 66-67 0.0 0.0 0.0 1.060413744113019 3.441672597815777E-4 68-69 0.0 0.0 0.0 1.2179735156410252 3.441672597815777E-4 70-71 0.0 0.0 0.0 1.387647974713343 3.441672597815777E-4 72-73 0.0 0.0 0.0 1.5604543558496733 4.818341636942088E-4 74-75 0.0 0.0 0.0 1.7388706633204432 4.818341636942088E-4 76-77 0.0 0.0 0.0 1.9356999191895272 4.818341636942088E-4 78-79 0.0 0.0 0.0 2.1682537366239396 4.818341636942088E-4 80-81 0.0 6.883345195631554E-5 0.0 2.415296995695156 4.818341636942088E-4 82-83 0.0 6.883345195631554E-5 0.0 2.677965448360456 5.506676156505243E-4 84-85 0.0 6.883345195631554E-5 0.0 2.9487562483566014 5.506676156505243E-4 86-87 0.0 6.883345195631554E-5 0.0 3.230560400665757 5.506676156505243E-4 88 0.0 6.883345195631554E-5 0.0 3.45674712379421 5.506676156505243E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3035 0.0 52.819763 1 GTATCAA 5115 0.0 36.85526 1 ATCAACG 6560 0.0 28.580872 3 TCAACGC 6555 0.0 28.387613 4 CAACGCA 6615 0.0 28.13013 5 TATCAAC 6695 0.0 28.086344 2 GTACCTG 1300 0.0 27.845058 1 AACGCAG 6810 0.0 27.324642 6 TACCTGG 1300 0.0 27.120876 2 GTACATG 5775 0.0 24.58418 1 ACGCAGA 7680 0.0 24.229273 7 CGCAGAG 7765 0.0 23.90353 8 TACATGG 5960 0.0 23.66251 2 ACATGGG 5885 0.0 22.516924 3 CATGGGG 3610 0.0 22.388638 4 GTATCGC 105 0.002338649 22.37624 6 GCAGAGT 8640 0.0 21.210814 9 ACCTGGG 1600 0.0 21.145548 3 GAGTACT 5150 0.0 20.803385 12-13 ATGGGGG 2250 0.0 20.4668 5 >>END_MODULE