FastQCFastQC Report
Mon 6 Jun 2016
ERR1211557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3777552
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152550.4038329584874014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138740.36727489125232426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88950.23546995514555458No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41110.10882709225445474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.057.335931
GTATCAA120400.039.9978561
TATAACG3000.032.9116862
TCAACGC147300.031.7409064
ATCAACG147000.031.7114663
CAACGCA150300.031.1052955
TATCAAC153200.030.6590582
GTACCTG34000.030.4601021
AACGCAG155450.030.2505386
TACCTGG32950.030.1078072
GTACATG134550.028.1293661
ACGCAGA172350.026.9586247
TACATGG135200.026.9511382
CGCAGAG174900.026.2976598
ACATGGG138100.025.7927443
ACCTGGG39700.025.0938533
ATGGGGG59400.024.9169185
CATGGGG90150.024.9150144
TAACGCA3950.023.7920514
GCAGAGT192500.023.334449
Produced by FastQC (version 0.11.5)