##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211557_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3777552
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36599575598165	31.0	31.0	34.0	28.0	34.0
2	31.397607498189302	31.0	31.0	34.0	28.0	34.0
3	31.489998284603363	33.0	31.0	34.0	28.0	34.0
4	35.05091869019937	37.0	35.0	37.0	32.0	37.0
5	35.134639046663025	37.0	35.0	37.0	32.0	37.0
6	35.25353297585315	37.0	35.0	37.0	32.0	37.0
7	35.1877281901083	37.0	35.0	37.0	32.0	37.0
8	35.160284226398474	37.0	35.0	37.0	32.0	37.0
9	36.72896786066744	39.0	37.0	39.0	32.0	39.0
10-11	36.71540285878262	39.0	37.0	39.0	32.0	39.0
12-13	36.653338458345516	39.0	37.0	39.0	32.0	39.0
14-15	37.83439433792043	40.0	38.0	41.0	32.0	41.0
16-17	37.811609211468166	40.0	38.0	41.0	32.0	41.0
18-19	37.8280133536216	40.0	38.0	41.0	32.0	41.0
20-21	37.790684681508026	40.0	38.0	41.0	32.0	41.0
22-23	37.65049336183857	40.0	37.0	41.0	32.0	41.0
24-25	37.565105523365396	40.0	37.0	41.0	32.0	41.0
26-27	37.419429699445566	40.0	37.0	41.0	31.0	41.0
28-29	37.25435705451573	40.0	36.5	41.0	31.0	41.0
30-31	37.065213397459516	39.5	36.0	41.0	30.0	41.0
32-33	36.88091983909156	39.0	36.0	41.0	30.0	41.0
34-35	36.70826040250405	39.0	35.5	41.0	30.0	41.0
36-37	36.53871793161285	39.0	35.0	41.0	30.0	41.0
38-39	36.40539852264112	39.0	35.0	40.0	29.0	41.0
40-41	36.3648987492429	39.0	35.0	40.0	29.0	41.0
42-43	36.30168439772636	39.0	35.0	40.0	29.0	41.0
44-45	36.11366951401331	39.0	35.0	40.0	28.0	41.0
46-47	36.04170941922176	39.0	35.0	40.0	27.5	41.0
48-49	35.99632870705685	39.0	35.0	41.0	27.0	41.0
50-51	35.00891463572176	38.0	34.0	40.0	25.5	40.5
52-53	35.21520325861828	38.0	34.5	39.5	26.5	40.5
54-55	35.797242235183	39.0	35.0	40.5	27.0	41.0
56-57	35.76105226347645	39.0	35.0	41.0	27.0	41.0
58-59	35.59846919380594	38.5	34.5	40.5	26.5	41.0
60-61	35.40526152915963	38.0	34.0	40.0	26.0	41.0
62-63	35.141034061212125	38.0	34.0	40.0	26.0	41.0
64-65	34.857212951668174	37.0	34.0	40.0	26.0	41.0
66-67	34.54664224344232	37.0	34.0	40.0	26.0	41.0
68-69	34.26143875716337	36.0	33.0	39.0	25.5	41.0
70-71	33.916313130831824	36.0	33.0	39.0	25.0	41.0
72-73	33.56835087908783	35.0	33.0	38.5	24.5	40.0
74-75	33.204498971820904	35.0	32.5	37.5	24.0	40.0
76-77	32.86443601570541	35.0	32.0	37.0	24.0	39.0
78-79	32.546918612900626	35.0	32.0	36.5	23.0	39.0
80-81	32.21956507812467	35.0	32.0	36.0	22.5	38.5
82-83	31.921962291981686	35.0	31.0	36.0	22.0	37.0
84-85	31.662876778400403	35.0	31.0	35.0	21.5	37.0
86-87	31.374362947220845	34.5	31.0	35.0	20.0	36.5
88-89	31.159441881938356	34.0	31.0	35.0	20.0	36.0
90-91	30.932545998043175	34.0	30.5	35.0	20.0	36.0
92-93	30.784654718187863	34.0	30.0	35.0	19.0	35.5
94-95	30.62112553314951	34.0	30.0	35.0	19.0	35.0
96-97	30.514113240532495	34.0	30.0	35.0	19.0	35.0
98-99	30.341123960702593	34.0	30.0	35.0	18.0	35.0
100	28.956338390576754	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37683673741302925
1101	2	0.40901877277891074
1101	3	0.3477481246797396
1101	4	0.28473177291276386
1101	5	0.28610815712779214
1101	6	0.2816221256049829
1101	7	0.2831006021833815
1101	8	0.344978404816878
1101	9	0.3350623609741561
1101	10-11	0.34785537293605273
1101	12-13	0.3835668449817007
1101	14-15	0.46074365884070545
1101	16-17	0.46893081686588545
1101	18-19	0.37474147981010475
1101	20-21	0.47284922839835986
1101	22-23	0.3827647999372914
1101	24-25	0.4259875908601032
1101	26-27	0.37346392591229005
1101	28-29	0.3998726909929218
1101	30-31	0.4310990088484985
1101	32-33	0.41511960478040777
1101	34-35	0.382898559280882
1101	36-37	0.41577291616822265
1101	38-39	0.37611319600214443
1101	40-41	0.35010989861884667
1101	42-43	0.26914100913881356
1101	44-45	0.2931050322601507
1101	46-47	0.2863749978846357
1101	48-49	0.2731165312530024
1101	50-51	0.2029187581285612
1101	52-53	0.3855711713233987
1101	54-55	0.47952582437118707
1101	56-57	0.35187246633221037
1101	58-59	0.3731873007046218
1101	60-61	0.39302375807729817
1101	62-63	0.3607381961024032
1101	64-65	0.3568109515762572
1101	66-67	0.3299980696473739
1101	68-69	0.2594700034592847
1101	70-71	0.23686413351716595
1101	72-73	0.194798604622477
1101	74-75	0.08556964274286116
1101	76-77	0.15522293221381744
1101	78-79	0.08554451468914692
1101	80-81	0.10154791158394261
1101	82-83	0.060872894674005806
1101	84-85	-0.039839069409215
1101	86-87	-0.04458481439860762
1101	88-89	-0.02938035306413056
1101	90-91	-0.030642319600776347
1101	92-93	3.5474648150923827E-4
1101	94-95	-0.09326713265809161
1101	96-97	-0.18989220722146882
1101	98-99	-0.2301329258831224
1101	100	-0.26873467987707045
1102	1	0.41019526465679235
1102	2	0.4744021355120758
1102	3	0.4236332953919444
1102	4	0.3850836627455223
1102	5	0.4695433615725122
1102	6	0.42053761290110714
1102	7	0.42822308874741566
1102	8	0.44760291175619926
1102	9	0.5567725946020801
1102	10-11	0.48508412073930174
1102	12-13	0.5180539103228909
1102	14-15	0.44279140837706876
1102	16-17	0.48501798764461057
1102	18-19	0.5354931683943036
1102	20-21	0.5395299848105211
1102	22-23	0.5913611831618653
1102	24-25	0.5948499836360455
1102	26-27	0.4596841434691612
1102	28-29	0.5642667310930207
1102	30-31	0.4189853279307698
1102	32-33	0.5106838948303576
1102	34-35	0.539598599364858
1102	36-37	0.5388786759888262
1102	38-39	0.5546358149534072
1102	40-41	0.49855705049419186
1102	42-43	0.5389016359608902
1102	44-45	0.4288762661801613
1102	46-47	0.4278067733390074
1102	48-49	0.46912664907899426
1102	50-51	0.5119150218391013
1102	52-53	0.5990150646021775
1102	54-55	0.6000967922453171
1102	56-57	0.630236276237234
1102	58-59	0.6258461418203112
1102	60-61	0.6481910828393254
1102	62-63	0.7039722575579148
1102	64-65	0.5314745941411942
1102	66-67	0.47404972041799454
1102	68-69	0.47668251106655646
1102	70-71	0.5028901169225577
1102	72-73	0.48300160525581504
1102	74-75	0.5235329966146409
1102	76-77	0.4263989569348752
1102	78-79	0.4006288652862082
1102	80-81	0.42021861987149833
1102	82-83	0.3142954427546236
1102	84-85	0.18637135930312354
1102	86-87	0.18779719528761518
1102	88-89	0.14913795212055092
1102	90-91	0.12758167204777138
1102	92-93	0.17288333000970724
1102	94-95	0.08337059733803542
1102	96-97	0.0022779772272407683
1102	98-99	-0.02168491352486157
1102	100	0.1076469032003402
1103	1	0.42583331171707783
1103	2	0.4432067343699728
1103	3	0.38848193244283635
1103	4	0.3766093526275043
1103	5	0.3277146202695036
1103	6	0.3398593058902293
1103	7	0.33777548685906567
1103	8	0.3575046164756017
1103	9	0.42283520249598183
1103	10-11	0.42694494437849073
1103	12-13	0.40964871076079135
1103	14-15	0.5434818605981704
1103	16-17	0.5093508177548287
1103	18-19	0.4250890409943011
1103	20-21	0.41278214796118107
1103	22-23	0.4657397240104544
1103	24-25	0.4670175196816828
1103	26-27	0.49291351773064207
1103	28-29	0.49291450933679215
1103	30-31	0.49072342030061833
1103	32-33	0.47090154006941276
1103	34-35	0.4572744536072051
1103	36-37	0.5000446643110408
1103	38-39	0.5538425536882272
1103	40-41	0.44690867289873637
1103	42-43	0.4008273819153203
1103	44-45	0.4172594001097636
1103	46-47	0.37591519925626216
1103	48-49	0.337789370424467
1103	50-51	0.38443008787152166
1103	52-53	0.4586187096323897
1103	54-55	0.5119363033100512
1103	56-57	0.4995172043715357
1103	58-59	0.4800944494742083
1103	60-61	0.3771157159413079
1103	62-63	0.3868310573392577
1103	64-65	0.34816811452940755
1103	66-67	0.4474634758428522
1103	68-69	0.39549987294029165
1103	70-71	0.3872232370231643
1103	72-73	0.32748440051560124
1103	74-75	0.3816233904021047
1103	76-77	0.35702245232317154
1103	78-79	0.3097973413362709
1103	80-81	0.30290039943134417
1103	82-83	0.33545006987225534
1103	84-85	0.215126514226192
1103	86-87	0.25203691555806884
1103	88-89	0.25328837368978085
1103	90-91	0.1835254033667475
1103	92-93	0.13079956336234133
1103	94-95	0.1499022446313205
1103	96-97	0.09245008824381884
1103	98-99	0.1479069691282895
1103	100	0.18299723852895156
1104	1	0.4513773828455534
1104	2	0.46603913886731263
1104	3	0.40011895143075904
1104	4	0.4221865763887749
1104	5	0.38756592000655843
1104	6	0.3598776292771575
1104	7	0.3463499190147985
1104	8	0.4692954657187087
1104	9	0.4854046566592487
1104	10-11	0.47033491230097013
1104	12-13	0.4804833841327536
1104	14-15	0.5056480519566051
1104	16-17	0.5651669985937744
1104	18-19	0.48911042893756473
1104	20-21	0.4937446749202792
1104	22-23	0.5751920127655268
1104	24-25	0.624677775171044
1104	26-27	0.6383761971529296
1104	28-29	0.5456301613277574
1104	30-31	0.526036952265521
1104	32-33	0.4421881231238487
1104	34-35	0.46726310923411063
1104	36-37	0.5295474489653671
1104	38-39	0.48835863137123425
1104	40-41	0.37776853050090153
1104	42-43	0.23722129395379454
1104	44-45	0.33861353733033894
1104	46-47	0.42396479427883094
1104	48-49	0.47199844796196544
1104	50-51	0.5599247127333058
1104	52-53	0.6036573653056365
1104	54-55	0.5874126464921616
1104	56-57	0.6049420205584042
1104	58-59	0.6426317311251495
1104	60-61	0.6666581896819608
1104	62-63	0.6129259075129028
1104	64-65	0.6075213138001843
1104	66-67	0.5182454358798907
1104	68-69	0.44622510416067485
1104	70-71	0.3985702168155427
1104	72-73	0.5031201349201027
1104	74-75	0.44253474951175775
1104	76-77	0.35179172547695714
1104	78-79	0.45883676534982243
1104	80-81	0.4575407021703981
1104	82-83	0.4289399498387816
1104	84-85	0.3112018963804921
1104	86-87	0.29724416122028074
1104	88-89	0.29742270024275186
1104	90-91	0.2482008421502293
1104	92-93	0.3811334531013344
1104	94-95	0.3761060550060442
1104	96-97	0.3990478166813993
1104	98-99	0.28893813695920656
1104	100	0.3091619135970056
1105	1	0.40107714974354636
1105	2	0.4125604151388984
1105	3	0.315378563079765
1105	4	0.2984458795204219
1105	5	0.3004273804744386
1105	6	0.3107119592612335
1105	7	0.28452170605402216
1105	8	0.27944349692086234
1105	9	0.36072602891401573
1105	10-11	0.34728599896769197
1105	12-13	0.36456203130315146
1105	14-15	0.40177125512862943
1105	16-17	0.3811316295536855
1105	18-19	0.4608975157718689
1105	20-21	0.4896849101552476
1105	22-23	0.4968595491210692
1105	24-25	0.44698748470765537
1105	26-27	0.521884944191541
1105	28-29	0.5169268579066397
1105	30-31	0.4058658679602942
1105	32-33	0.4868852904773746
1105	34-35	0.48901310732968284
1105	36-37	0.4417675932448404
1105	38-39	0.38085485108130257
1105	40-41	0.4206948177039038
1105	42-43	0.5244366771628322
1105	44-45	0.5452473272185827
1105	46-47	0.5206966526960244
1105	48-49	0.589015561153559
1105	50-51	0.5535310712380976
1105	52-53	0.5311707820021425
1105	54-55	0.507873965651477
1105	56-57	0.44644622363139064
1105	58-59	0.5360037497428465
1105	60-61	0.5444462725321983
1105	62-63	0.5618641411938583
1105	64-65	0.5312130192536983
1105	66-67	0.48104600225683214
1105	68-69	0.48369115772842974
1105	70-71	0.47445906105616587
1105	72-73	0.4699121447012402
1105	74-75	0.46737158762774555
1105	76-77	0.478671158033535
1105	78-79	0.49574791615768277
1105	80-81	0.43124197737550674
1105	82-83	0.47481595823350276
1105	84-85	0.427965958678147
1105	86-87	0.43854011507217905
1105	88-89	0.48131856991892263
1105	90-91	0.4354520762196863
1105	92-93	0.46877666610020086
1105	94-95	0.4320536318116339
1105	96-97	0.3471573860520998
1105	98-99	0.28822421444658275
1105	100	0.3855661437128717
1106	1	0.24853196847208991
1106	2	0.24849915339073547
1106	3	0.24504893521120508
1106	4	0.17105090050394267
1106	5	0.17209483716116125
1106	6	0.20707990974740653
1106	7	0.23721500993265465
1106	8	0.22230911131034503
1106	9	0.2595492558973618
1106	10-11	0.2792644725209712
1106	12-13	0.2482353624411644
1106	14-15	0.3151664450632339
1106	16-17	0.2631115551687415
1106	18-19	0.31692303686516965
1106	20-21	0.2948743328450192
1106	22-23	0.35448267657665156
1106	24-25	0.3334217944171769
1106	26-27	0.35969158149210756
1106	28-29	0.3442819729050157
1106	30-31	0.4107242144114096
1106	32-33	0.4307305235800527
1106	34-35	0.4343046493405396
1106	36-37	0.5176194858966241
1106	38-39	0.5443028936035788
1106	40-41	0.366903927398063
1106	42-43	0.3339928680159261
1106	44-45	0.35917588528678124
1106	46-47	0.5589238095492064
1106	48-49	0.6297385722473123
1106	50-51	0.5150108870871293
1106	52-53	0.5852493466661457
1106	54-55	0.5295434343051539
1106	56-57	0.6408407175607849
1106	58-59	0.5398154734435394
1106	60-61	0.46586936444918337
1106	62-63	0.6022716672546764
1106	64-65	0.7256824543376794
1106	66-67	0.7830825127505676
1106	68-69	0.6966509383243533
1106	70-71	0.6859648109747098
1106	72-73	0.5602880391959104
1106	74-75	0.6149192460181538
1106	76-77	0.5059447852937069
1106	78-79	0.4998470373403663
1106	80-81	0.47936705797724244
1106	82-83	0.4499867170071532
1106	84-85	0.47877701516876314
1106	86-87	0.5681560410396678
1106	88-89	0.5862000078440239
1106	90-91	0.6975734388049268
1106	92-93	0.4973594562639825
1106	94-95	0.6056314665069493
1106	96-97	0.604902535919642
1106	98-99	0.6004287260271433
1106	100	0.5182350603369592
1107	1	0.3330252831359033
1107	2	0.34233585279946155
1107	3	0.40377190329913404
1107	4	0.3248433759544369
1107	5	0.3105220918473748
1107	6	0.3251929715842081
1107	7	0.31316307599685445
1107	8	0.3474390834257406
1107	9	0.29387685391410656
1107	10-11	0.45322761711049964
1107	12-13	0.42101203849186675
1107	14-15	0.5121243583851154
1107	16-17	0.5083684089318936
1107	18-19	0.45501352561663566
1107	20-21	0.43541150047015265
1107	22-23	0.49340518046085435
1107	24-25	0.4405453047097083
1107	26-27	0.5427299828403704
1107	28-29	0.43031568152952815
1107	30-31	0.4708813284355813
1107	32-33	0.5349190575931502
1107	34-35	0.6451537394829288
1107	36-37	0.6558344029291803
1107	38-39	0.6017938633134747
1107	40-41	0.6200171863489814
1107	42-43	0.6317505366347262
1107	44-45	0.7011949990195134
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2316	54-55	-0.4175508464351907
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2316	58-59	-0.4556075130146908
2316	60-61	-0.3011372623459252
2316	62-63	-0.41298225408146294
2316	64-65	-0.07419300149955887
2316	66-67	-0.06347923432831237
2316	68-69	-0.06514705365776052
2316	70-71	-0.16455396743440076
2316	72-73	-0.2223772619040787
2316	74-75	-0.0962976021120383
2316	76-77	-0.0827842169401265
2316	78-79	-0.3375388360777052
2316	80-81	-0.05344609652536292
2316	82-83	-0.01415567412874097
2316	84-85	0.020725462225669844
2316	86-87	0.25491648616212714
2316	88-89	0.04630647723174164
2316	90-91	-0.04027342229005271
2316	92-93	0.17630060058894514
2316	94-95	0.20158238079554636
2316	96-97	0.036814876995126866
2316	98-99	0.2149763369389781
2316	100	-0.09277878889924907
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	8.0
1	30.0
2	59.0
3	55.0
4	67.0
5	73.0
6	136.0
7	1060.0
8	1966.0
9	2470.0
10	3309.0
11	4950.0
12	6919.0
13	8330.0
14	9410.0
15	10559.0
16	12435.0
17	15218.0
18	18453.0
19	22439.0
20	26304.0
21	31711.0
22	36531.0
23	39243.0
24	38897.0
25	39589.0
26	42191.0
27	47076.0
28	54663.0
29	64819.0
30	78597.0
31	96883.0
32	122070.0
33	160370.0
34	223247.0
35	316289.0
36	479520.0
37	764233.0
38	799393.0
39	197716.0
40	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.85204336098259	18.611426127476495	12.96467712730176	23.57185338423915
2	15.9298090338275	20.259930227720503	40.564829524300386	23.24543121415161
3	18.183409378997627	26.396552961385254	30.599617260507795	24.820420399109324
4	12.368524465835955	16.628702499332693	38.13457798604373	32.86819504878762
5	12.708002731577015	37.672143501529035	35.268774097574706	14.351079669319248
6	30.166048436882853	37.01055632209225	18.725611867470455	14.097783373554448
7	26.50854223997692	32.92998765025196	23.138292028522226	17.4231780812489
8	25.664589735498367	35.45566648523522	20.890445410666896	17.989298368599513
9	26.918337129207355	15.291234826983066	20.717318265968153	37.07310977784143
10-11	24.56748357521673	25.916331755604627	29.30828081223687	20.207903856941773
12-13	25.027447035230427	24.059857966391114	29.02428337889087	21.888411619487595
14-15	22.661607116025685	25.251679062909936	26.82199490664724	25.264718914417138
16-17	21.954470038909268	28.034953480694547	27.54370972101654	22.466866759379645
18-19	21.79631901970438	27.110781152222263	29.526517967174943	21.566381860898414
20-21	22.537404560749764	26.53737384756038	29.443781492602756	21.481440099087106
22-23	22.335012945029675	26.253124028758613	29.366671491285164	22.045191534926552
24-25	21.971271784874762	26.37416258352984	29.431481471430445	22.223084160164955
26-27	21.80529487116525	26.51789355746153	29.341386247186563	22.33542532418666
28-29	21.934015378926404	26.826600016443656	29.279922595641	21.959462008988936
30-31	22.51765496429293	26.575285088654095	29.26622915697529	21.64083079007769
32-33	22.188985357436334	26.63114860117664	29.24325285393168	21.936613187455343
34-35	22.3257836722914	26.802545561418885	29.11932683218729	21.752343934102424
36-37	22.31354442822674	26.74918497777513	29.099057056520344	21.83821353747778
38-39	22.22552014422834	27.02572211473821	28.901641050108967	21.847116690924487
40-41	22.276751557402562	26.67319906565776	29.14852019449973	21.901529182439948
42-43	22.12256528166632	26.86116387166309	28.77632666950459	22.239944177166
44-45	21.844614621725565	27.11131973165631	28.93890632665343	22.1051593199647
46-47	21.971232776423516	26.8190343398521	28.614619369203286	22.5951135145211
48-49	21.99084495827359	27.004023913406368	28.601556057917772	22.40357507040227
50-51	22.03617227221365	27.180460523598576	28.548629474738767	22.234737729449005
52-53	22.147517372573436	27.331229299964292	28.209188171358996	22.312065156103277
54-55	21.985178885134307	27.15987238689907	28.525277490443386	22.32967123752324
56-57	22.058188269966863	27.293509511225896	28.166610248048197	22.481691970759048
58-59	22.078210426600524	27.25052081368077	28.444681875021928	22.226586884696783
60-61	22.390492618879005	27.352640054356335	28.37525044283019	21.88161688393447
62-63	22.26672592449765	27.324847950956553	28.42690058324644	21.981525541299362
64-65	22.14551458955164	27.052281516202765	28.349577782894464	22.452626111351133
66-67	22.017117159219342	27.101414144195658	28.281070177916835	22.600398518668168
68-69	21.835298572476415	27.24802527227808	28.40753401986511	22.509142135380394
70-71	21.91706486065252	27.107228007079105	28.417113524515	22.558593607753377
72-73	21.862439259893964	27.56401471229082	27.96606432714389	22.60748170067133
74-75	21.96516973523882	27.47861542295501	27.95981640739106	22.596398434415114
76-77	21.907360418523997	27.21966870027572	28.081915936686542	22.791054944513746
78-79	21.862962711436296	27.225987841200887	28.197926599487865	22.71312284787495
80-81	22.01625210287182	27.409970210612205	27.85009216426965	22.723685522246324
82-83	22.24000440575698	27.397003846961898	27.91041751034221	22.45257423693891
84-85	22.134650866401728	27.5518145844302	27.707605517975065	22.605929031193003
86-87	21.89120124467924	27.55084046563205	27.961541190812333	22.59641709887638
88-89	21.792491409200842	27.26535703397041	28.092644671414263	22.849506885414485
90-91	22.303933186017044	27.193637948817077	27.95199466166021	22.550434203505667
92-93	22.120657820629567	27.29977590545397	27.897176006234293	22.682390267682166
94-95	22.023864527264873	27.18670129545525	27.832840281729148	22.956593895550725
96-97	21.98264940838703	27.125807542241333	27.890704034130366	23.000839015241272
98-99	21.955512015594955	27.019649245901856	27.808505075310524	23.21633366319266
100	22.058653722903323	27.082801454690298	27.847117685631385	23.011427136774998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1893.0
1	1400.5
2	1968.0
3	2723.5
4	1806.5
5	1399.0
6	2045.5
7	3887.5
8	6221.0
9	7168.0
10	5869.5
11	4409.5
12	4265.5
13	4413.0
14	4660.5
15	4925.0
16	5303.5
17	5743.0
18	6134.5
19	6605.5
20	7288.0
21	8150.0
22	9501.5
23	11566.5
24	14201.0
25	17604.5
26	21761.5
27	26479.5
28	31527.5
29	37571.0
30	44874.5
31	51733.5
32	59151.0
33	67703.5
34	76234.5
35	83594.0
36	92709.5
37	101934.0
38	108437.0
39	114751.5
40	117633.0
41	119605.5
42	121789.5
43	124055.0
44	127119.5
45	131070.0
46	134505.0
47	135850.0
48	138609.0
49	142014.0
50	144030.0
51	142484.0
52	135935.5
53	128477.5
54	122661.0
55	113921.0
56	103312.5
57	93691.0
58	83499.0
59	71475.5
60	58856.5
61	47955.5
62	38660.0
63	30393.5
64	23183.0
65	17587.0
66	13383.0
67	10335.0
68	8111.0
69	6235.5
70	4871.0
71	3859.0
72	3042.0
73	2409.0
74	1894.5
75	1476.0
76	1141.5
77	831.5
78	608.5
79	464.0
80	354.0
81	304.0
82	250.5
83	173.5
84	141.5
85	121.0
86	92.5
87	83.0
88	71.0
89	51.5
90	44.0
91	35.0
92	23.0
93	15.5
94	14.0
95	14.5
96	12.0
97	12.0
98	11.0
99	7.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16261854237876805
2	0.10183843928554788
3	0.02893408217808782
4	0.03134304967873374
5	0.025916254759696226
6	0.0026472170336768364
7	0.003891409039504949
8	0.004870879341965379
9	0.005426794919037514
10-11	0.004791462830955074
12-13	0.007332791183284836
14-15	0.01769664587012965
16-17	0.008021067612040814
18-19	0.01722014680406782
20-21	0.01781577063664511
22-23	0.04708075494394253
24-25	0.01412290287466592
26-27	0.009754994769099141
28-29	0.027014849828672114
30-31	0.0150626649216212
32-33	0.020873306310541854
34-35	0.02070123720335286
36-37	0.02156158273929783
38-39	0.020198265966954263
40-41	0.02624715688890583
42-43	0.03493002875936586
44-45	0.037709606644726536
46-47	0.020873306310541854
48-49	0.013434626445909943
50-51	0.012177198354913446
52-53	0.022170442657043505
54-55	0.025955963015201377
56-57	0.019205559579325446
58-59	0.026723655954967663
60-61	0.02624715688890583
62-63	0.051911926030402754
64-65	0.03273283862141408
66-67	0.0215880549096346
68-69	0.028642888304383367
70-71	0.04366584497049941
72-73	0.04038329584874013
74-75	0.04190544564310432
76-77	0.022527816956589876
78-79	0.016320093012617696
80-81	0.023070496448493627
82-83	0.01788195106248703
84-85	0.018411394469222395
86-87	0.02213073440153835
88-89	0.018305505787875324
90-91	0.029039970859434893
92-93	0.027028085913840497
94-95	0.019404100856851212
96-97	0.013368446020068023
98-99	0.018252561447201786
100	0.04309669330825889
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3777552.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.66660831602672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0836776909707	50.49406601116876
2	14.033630841971279	18.15014617818938
3	3.9302075443663687	7.624595756166995
4	1.488257684983894	3.849623071526806
5	0.7624318576540994	2.465194115328914
6	0.43388202717618873	1.6834607464059754
7	0.2732977027491958	1.2371264848146502
8	0.19820640721212346	1.0253868880730626
9	0.1386524152723514	0.8069563287439405
>10	0.6034157268770537	6.713949828976055
>50	0.03394417113990967	1.5002767417027947
>100	0.018852476645416488	2.3064931375121565
>500	0.0010953537298638988	0.46099280984034996
>1k	2.987328343252093E-4	0.4574833647143538
>5k	4.978880572086822E-5	0.2863899309686943
>10k+	9.957761144173643E-5	0.9378586058670149
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15255	0.4038329584874014	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13874	0.36727489125232426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8895	0.23546995514555458	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4111	0.10882709225445474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.2944340673536724E-5
2	0.0	0.0	0.0	0.0	5.2944340673536724E-5
3	0.0	0.0	0.0	0.0	5.2944340673536724E-5
4	0.0	0.0	0.0	0.0	7.941651101030509E-5
5	0.0	0.0	0.0	0.0	7.941651101030509E-5
6	0.0	0.0	0.0	0.0	1.3236085168384182E-4
7	0.0	0.0	0.0	0.0	1.3236085168384182E-4
8	0.0	0.0	0.0	0.0	1.3236085168384182E-4
9	0.0	0.0	0.0	0.0	1.5883302202061018E-4
10-11	0.0	0.0	0.0	0.0	1.5883302202061018E-4
12-13	0.0	0.0	0.0	0.0	1.5883302202061018E-4
14-15	0.0	1.3236085168384181E-5	0.0	2.6472170336768362E-5	1.9854127752576273E-4
16-17	0.0	2.6472170336768362E-5	0.0	6.618042584192091E-5	2.9119387370445197E-4
18-19	0.0	2.6472170336768362E-5	0.0	7.941651101030509E-5	3.1766604404122035E-4
20-21	0.0	2.6472170336768362E-5	0.0	1.0588868134707345E-4	3.1766604404122035E-4
22-23	0.0	2.6472170336768362E-5	0.0	1.7206910718899434E-4	3.7061038471475707E-4
24-25	0.0	2.6472170336768362E-5	0.0	3.573742995463729E-4	4.632629808934463E-4
26-27	0.0	2.6472170336768362E-5	0.0	5.691516622405197E-4	4.764990660618305E-4
28-29	0.0	2.6472170336768362E-5	0.0	0.0015221497943641808	4.764990660618305E-4
30-31	0.0	2.6472170336768362E-5	0.0	0.00452674112758739	4.764990660618305E-4
32-33	0.0	2.6472170336768362E-5	0.0	0.012468392228617899	5.294434067353673E-4
34-35	0.0	2.6472170336768362E-5	0.0	0.02402349458061729	5.823877474089039E-4
36-37	0.0	2.6472170336768362E-5	0.0	0.04349377586331042	5.823877474089039E-4
38-39	0.0	2.6472170336768362E-5	0.0	0.07604130929236712	5.823877474089039E-4
40-41	0.0	2.6472170336768362E-5	0.0	0.11573632871235129	6.088599177456723E-4
42-43	0.0	2.6472170336768362E-5	0.0	0.1536312405494352	6.220960029140565E-4
44-45	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.1998781221277695	6.353320880824407E-4
46-47	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.25598588715655	6.618042584192091E-4
48-49	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.3190293608135639	7.412207694295141E-4
50-51	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.3873275602824263	7.412207694295141E-4
52-53	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.4608672494779688	7.676929397662825E-4
54-55	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.5445193077421568	7.941651101030509E-4
56-57	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.6414339233450658	7.941651101030509E-4
58-59	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.7521008314379259	8.206372804398193E-4
60-61	2.6472170336768362E-5	2.6472170336768362E-5	0.0	0.8717418052749506	8.471094507765876E-4
62-63	2.6472170336768362E-5	2.6472170336768362E-5	0.0	1.0031893670821739	9.000537914501243E-4
64-65	2.6472170336768362E-5	2.6472170336768362E-5	0.0	1.138210671884861	9.000537914501243E-4
66-67	2.6472170336768362E-5	2.6472170336768362E-5	0.0	1.292741966225746	9.397620469552769E-4
68-69	2.6472170336768362E-5	2.6472170336768362E-5	0.0	1.4726733080047607	9.794703024604294E-4
70-71	2.6472170336768362E-5	2.6472170336768362E-5	0.0	1.6690571036480768	9.794703024604294E-4
72-73	2.6472170336768362E-5	2.6472170336768362E-5	0.0	1.8649908723956679	0.0011383033244810395
74-75	2.6472170336768362E-5	2.6472170336768362E-5	0.0	2.0741474902264745	0.0011383033244810395
76-77	2.6472170336768362E-5	5.2944340673536724E-5	0.0	2.3064275488464485	0.0011383033244810395
78-79	2.6472170336768362E-5	5.2944340673536724E-5	0.0	2.5747891756354377	0.0011383033244810395
80-81	2.6472170336768362E-5	5.2944340673536724E-5	0.0	2.8572869413842614	0.0012044837503229606
82-83	2.6472170336768362E-5	5.2944340673536724E-5	0.0	3.148705828536576	0.0012971363465016498
84-85	2.6472170336768362E-5	5.2944340673536724E-5	0.0	3.447722228575543	0.0012971363465016498
86-87	2.6472170336768362E-5	5.2944340673536724E-5	0.0	3.767982545309767	0.0012971363465016498
88	2.6472170336768362E-5	5.2944340673536724E-5	0.0	4.020037315171307	0.0013236085168384182
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6675	0.0	57.33593	1
GTATCAA	12040	0.0	39.997856	1
TATAACG	300	0.0	32.911686	2
TCAACGC	14730	0.0	31.740906	4
ATCAACG	14700	0.0	31.711466	3
CAACGCA	15030	0.0	31.105295	5
TATCAAC	15320	0.0	30.659058	2
GTACCTG	3400	0.0	30.460102	1
AACGCAG	15545	0.0	30.250538	6
TACCTGG	3295	0.0	30.107807	2
GTACATG	13455	0.0	28.129366	1
ACGCAGA	17235	0.0	26.958624	7
TACATGG	13520	0.0	26.951138	2
CGCAGAG	17490	0.0	26.297659	8
ACATGGG	13810	0.0	25.792744	3
ACCTGGG	3970	0.0	25.093853	3
ATGGGGG	5940	0.0	24.916918	5
CATGGGG	9015	0.0	24.915014	4
TAACGCA	395	0.0	23.792051	4
GCAGAGT	19250	0.0	23.33444	9
>>END_MODULE