FastQCFastQC Report
Mon 6 Jun 2016
ERR1211557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3777552
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163770.43353473360525546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154080.4078832005489269No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106640.2822992244712978No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.1041679902751835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74150.052.6362571
GTATCAA127950.037.8175281
TACCTGG31850.033.218912
GTACCTG35900.030.6506351
TCAACGC164300.029.086084
ATCAACG165950.028.7402523
CAACGCA166850.028.641555
TATCAAC170650.028.2993492
AACGCAG172750.027.7442116
GTACATG138700.026.9191971
TACATGG140450.025.6794622
ACCTGGG41900.024.6723443
ACATGGG140550.024.4727063
TATAACG2501.6370905E-1124.452072
ACGCAGA195800.024.3101167
CGCAGAG200450.023.7227338
CATGGGG93450.023.5325134
ATGGGGG60550.022.0397155
GCAGAGT218050.021.2692099
GAGTACT141350.020.3291712-13
Produced by FastQC (version 0.11.5)