##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211557_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3777552 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07770482047633 33.0 31.0 34.0 30.0 34.0 2 32.296111873509616 34.0 31.0 34.0 30.0 34.0 3 32.30031750721102 34.0 31.0 34.0 30.0 34.0 4 35.95570173488016 37.0 35.0 37.0 35.0 37.0 5 35.87536505122895 37.0 35.0 37.0 35.0 37.0 6 35.8971278224628 37.0 35.0 37.0 35.0 37.0 7 35.82288847380526 37.0 35.0 37.0 35.0 37.0 8 35.81768272150853 37.0 35.0 37.0 35.0 37.0 9 37.46129980474127 39.0 37.0 39.0 34.0 39.0 10-11 37.418003908351224 39.0 37.0 39.0 34.0 39.0 12-13 37.32767186262426 39.0 37.0 39.0 34.0 39.0 14-15 38.53493307305895 40.0 38.0 41.0 34.0 41.0 16-17 38.45848118040466 40.0 38.0 41.0 34.0 41.0 18-19 38.45561596504827 40.0 38.0 41.0 34.0 41.0 20-21 38.39752914056511 40.0 38.0 41.0 34.0 41.0 22-23 38.28768882599101 40.0 38.0 41.0 34.0 41.0 24-25 38.169634461683124 40.0 37.5 41.0 33.0 41.0 26-27 37.98749692922824 40.0 37.0 41.0 33.0 41.0 28-29 38.012659918381004 40.0 37.0 41.0 33.0 41.0 30-31 37.92128619275128 40.0 37.0 41.0 33.0 41.0 32-33 37.71304882103542 40.0 37.0 41.0 33.0 41.0 34-35 37.584797244352956 40.0 37.0 41.0 32.5 41.0 36-37 37.52271219562299 40.0 37.0 41.0 32.0 41.0 38-39 37.42119115765978 40.0 36.0 41.0 32.0 41.0 40-41 37.24401715714304 40.0 36.0 41.0 31.0 41.0 42-43 37.08760091191333 39.0 36.0 41.0 31.0 41.0 44-45 36.897243638208025 39.0 35.5 41.0 30.5 41.0 46-47 36.62373661567068 39.0 35.0 41.0 30.0 41.0 48-49 36.38198243200888 39.0 35.0 41.0 29.0 41.0 50-51 36.17350323701699 39.0 35.0 40.0 28.5 41.0 52-53 35.970300342655776 39.0 35.0 40.0 28.0 41.0 54-55 35.76507894530638 38.0 34.5 40.0 27.5 41.0 56-57 35.56727438828109 38.0 34.0 40.0 27.0 41.0 58-59 35.30324016717705 38.0 34.0 40.0 26.5 41.0 60-61 34.98033422173937 38.0 34.0 40.0 26.0 41.0 62-63 34.646806185593206 37.0 33.0 40.0 26.0 41.0 64-65 34.328546767853894 36.5 33.0 39.5 25.5 41.0 66-67 33.983278191802526 36.0 32.5 39.0 25.0 40.5 68-69 33.58479949448744 35.5 32.0 39.0 23.5 40.0 70-71 33.19620074058544 35.0 32.0 38.0 23.5 40.0 72-73 32.7852274435931 35.0 31.0 37.5 22.5 40.0 74-75 32.37326038132632 35.0 31.0 37.0 22.0 39.0 76-77 30.83194235314299 33.5 29.5 35.5 20.5 38.0 78-79 31.683015614344953 34.0 30.5 36.0 21.0 38.0 80-81 31.670320355616546 34.0 31.0 36.0 21.0 37.5 82-83 31.392167467185097 34.0 30.5 35.0 20.0 37.0 84-85 31.096445131661987 34.0 30.0 35.0 20.0 36.5 86-87 30.80608420479718 34.0 30.0 35.0 20.0 36.0 88-89 30.536139012778648 34.0 29.5 35.0 19.0 36.0 90-91 30.246246643328803 34.0 29.0 35.0 18.5 35.5 92-93 30.03674999047002 34.0 29.0 35.0 18.0 35.0 94-95 29.814528694773756 34.0 29.0 35.0 18.0 35.0 96-97 29.604623046883276 34.0 29.0 35.0 17.0 35.0 98-99 29.443262594399762 33.0 28.5 35.0 16.5 35.0 100 27.57874332371864 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35467893856711896 1101 2 0.2366374875222732 1101 3 0.2761241312124909 1101 4 0.2135052663916639 1101 5 0.15877904152451094 1101 6 0.2382838668723153 1101 7 0.21493590234643278 1101 8 0.21173599950502364 1101 9 0.24125366058323294 1101 10-11 0.23011202328904545 1101 12-13 0.26167158687851355 1101 14-15 0.27619050070986617 1101 16-17 0.2780442772090481 1101 18-19 0.3241981836008634 1101 20-21 0.270497773332778 1101 22-23 0.3622741499480213 1101 24-25 0.27217354174317165 1101 26-27 0.2521053954852377 1101 28-29 0.3749117297832356 1101 30-31 0.38797590259056136 1101 32-33 0.4116274446228587 1101 34-35 0.4479944148724613 1101 36-37 0.4584937310124104 1101 38-39 0.3913130306419248 1101 40-41 0.4482850989361751 1101 42-43 0.36278804023937994 1101 44-45 0.32783940023229263 1101 46-47 0.32427871870275027 1101 48-49 0.47858213380173 1101 50-51 0.4663323282358931 1101 52-53 0.32475627472619095 1101 54-55 0.3147758847639821 1101 56-57 0.2746202554114987 1101 58-59 0.3356034669927723 1101 60-61 0.22668682550689567 1101 62-63 0.2379655442379729 1101 64-65 0.1616006432083381 1101 66-67 0.1768049968942904 1101 68-69 0.22950622603771365 1101 70-71 0.27981253539438455 1101 72-73 0.2022245187352283 1101 74-75 0.1882582768208465 1101 76-77 0.2953001363734913 1101 78-79 0.35151587914971216 1101 80-81 0.2640748366442942 1101 82-83 0.3276322199446504 1101 84-85 0.33967724855603976 1101 86-87 0.17150516034994823 1101 88-89 0.2056528331230716 1101 90-91 0.13630442915118124 1101 92-93 0.14157704172029995 1101 94-95 0.006124566684469812 1101 96-97 -0.0462287046853298 1101 98-99 0.02957166961195412 1101 100 0.03440033647112983 1102 1 0.2877186671085994 1102 2 0.23510854107007617 1102 3 0.2608097490125445 1102 4 0.20653438987856987 1102 5 0.1979898174950634 1102 6 0.13061838312368934 1102 7 0.17824578506079547 1102 8 0.19905732868548398 1102 9 0.21789603355443887 1102 10-11 0.2213044187542792 1102 12-13 0.19167201144396273 1102 14-15 0.341860615400428 1102 16-17 0.331783506840722 1102 18-19 0.2899872333768485 1102 20-21 0.32535290626660185 1102 22-23 0.35862558320264526 1102 24-25 0.34440744960588887 1102 26-27 0.24130287256750194 1102 28-29 0.36188500611279295 1102 30-31 0.3689063865826796 1102 32-33 0.5161631488174407 1102 34-35 0.42732227782000365 1102 36-37 0.5142476882972673 1102 38-39 0.5031145651395192 1102 40-41 0.4351000430042191 1102 42-43 0.3290433741266128 1102 44-45 0.4573818390111484 1102 46-47 0.5151770231313861 1102 48-49 0.5405913364280863 1102 50-51 0.5244621492248029 1102 52-53 0.4455299612495409 1102 54-55 0.47880535482796915 1102 56-57 0.4737111120767281 1102 58-59 0.5785251720158229 1102 60-61 0.4978405718939811 1102 62-63 0.5450203291870679 1102 64-65 0.4903306862199912 1102 66-67 0.4589513273490198 1102 68-69 0.4996636626250677 1102 70-71 0.5132316370025478 1102 72-73 0.5347252194385064 1102 74-75 0.4506302161325024 1102 76-77 0.5144086295328236 1102 78-79 0.531155650125033 1102 80-81 0.5157954051487827 1102 82-83 0.37740565070705046 1102 84-85 0.2491686077977775 1102 86-87 0.3110991577210491 1102 88-89 0.34637181541017625 1102 90-91 0.315656937328324 1102 92-93 0.21312557153756373 1102 94-95 0.281876873386004 1102 96-97 0.47977033811257286 1102 98-99 0.41573639283217645 1102 100 0.40435179638028984 1103 1 0.2859086915079061 1103 2 0.2715982727166377 1103 3 0.32234507964228243 1103 4 0.15355709979407095 1103 5 0.1467535013450174 1103 6 0.2010276489700331 1103 7 0.21720759882804686 1103 8 0.20035145378962937 1103 9 0.29452473363984666 1103 10-11 0.26340609488728006 1103 12-13 0.23142255895842823 1103 14-15 0.36495491144990666 1103 16-17 0.3490226332612849 1103 18-19 0.3437629426583797 1103 20-21 0.30386613699224085 1103 22-23 0.30438992967490464 1103 24-25 0.30415967745993555 1103 26-27 0.33725398775249005 1103 28-29 0.42042681636577584 1103 30-31 0.5014406492912826 1103 32-33 0.5452660056583554 1103 34-35 0.5675013692376965 1103 36-37 0.5870807809462164 1103 38-39 0.5180380667058628 1103 40-41 0.5293155141176769 1103 42-43 0.6436790516330007 1103 44-45 0.6611019515182477 1103 46-47 0.7103540244404698 1103 48-49 0.6805169154947635 1103 50-51 0.6452548385326509 1103 52-53 0.6753703564984121 1103 54-55 0.6104878423999338 1103 56-57 0.6551890475331206 1103 58-59 0.8036703305460051 1103 60-61 0.7045185863928083 1103 62-63 0.6307057845184048 1103 64-65 0.6883301938094277 1103 66-67 0.7148453247427753 1103 68-69 0.6353748101881109 1103 70-71 0.6081440020209001 1103 72-73 0.5994622503059688 1103 74-75 0.5903201636786335 1103 76-77 0.6812041107718798 1103 78-79 0.6209960488125326 1103 80-81 0.6214848676200653 1103 82-83 0.6516280437650757 1103 84-85 0.6692594630482134 1103 86-87 0.6108398455622073 1103 88-89 0.5789417595451916 1103 90-91 0.568437205877764 1103 92-93 0.4949415005378306 1103 94-95 0.609650175861919 1103 96-97 0.6200301798190786 1103 98-99 0.5362131588134851 1103 100 0.5198969204325614 1104 1 0.22782323056397047 1104 2 0.23124511422629013 1104 3 0.2734428609090571 1104 4 0.11001715953739932 1104 5 0.09816188580062857 1104 6 0.1598000283874086 1104 7 0.22841155749522812 1104 8 0.1769696720742786 1104 9 0.1988281344493359 1104 10-11 0.250393547491619 1104 12-13 0.2779391227626533 1104 14-15 0.40345893249260456 1104 16-17 0.316593389617708 1104 18-19 0.2792529426491157 1104 20-21 0.17429675975568415 1104 22-23 0.25793586882495134 1104 24-25 0.2591803155994583 1104 26-27 0.368215483615586 1104 28-29 0.4412638183927484 1104 30-31 0.43745726129054674 1104 32-33 0.41342555258781744 1104 34-35 0.4190117279293517 1104 36-37 0.3608963181032081 1104 38-39 0.45659974802722303 1104 40-41 0.560839073155222 1104 42-43 0.6003285897483295 1104 44-45 0.5000146920648376 1104 46-47 0.5689904729658437 1104 48-49 0.6745830159313897 1104 50-51 0.6333004389271082 1104 52-53 0.677221579308771 1104 54-55 0.7151228274640999 1104 56-57 0.8245181817183251 1104 58-59 0.8220017113619704 1104 60-61 0.7033980515019138 1104 62-63 0.6136166426775915 1104 64-65 0.6625019920130555 1104 66-67 0.6989776841035962 1104 68-69 0.7758730519956458 1104 70-71 0.6905527280125838 1104 72-73 0.7811917041541179 1104 74-75 0.75257273539426 1104 76-77 0.6779046035662546 1104 78-79 0.6527960342613319 1104 80-81 0.7598257437875269 1104 82-83 0.6602593599811293 1104 84-85 0.6589027988314591 1104 86-87 0.7363503539365617 1104 88-89 0.6953312390293931 1104 90-91 0.5513878597084805 1104 92-93 0.6870617953803126 1104 94-95 0.6614206951357673 1104 96-97 0.5290717563903549 1104 98-99 0.5544685976711143 1104 100 0.6980185230291625 1105 1 0.2649633888600391 1105 2 0.150900622213463 1105 3 0.18477792219355393 1105 4 0.1009435114909607 1105 5 0.070677654465932 1105 6 0.11099381918977969 1105 7 0.16965423496889542 1105 8 0.13849212909133257 1105 9 0.1643703632397191 1105 10-11 0.20870204390748626 1105 12-13 0.22331798018192472 1105 14-15 0.20993236952839567 1105 16-17 0.26449537631718556 1105 18-19 0.27915105574167853 1105 20-21 0.213610822815113 1105 22-23 0.3309647380516765 1105 24-25 0.36630463857523665 1105 26-27 0.3285400553096878 1105 28-29 0.32383034959109125 1105 30-31 0.42235919276161127 1105 32-33 0.4403805909430005 1105 34-35 0.4675901199063759 1105 36-37 0.4468062634080283 1105 38-39 0.4772201821606714 1105 40-41 0.4435834303345345 1105 42-43 0.46552364128216084 1105 44-45 0.5178679920579796 1105 46-47 0.5609694975939092 1105 48-49 0.541066175073027 1105 50-51 0.6115959090542447 1105 52-53 0.5988788793628501 1105 54-55 0.564129287284473 1105 56-57 0.5276644875085523 1105 58-59 0.5604327862974046 1105 60-61 0.5802729610415724 1105 62-63 0.5958585640932696 1105 64-65 0.5900301680279298 1105 66-67 0.6018744621920078 1105 68-69 0.6732753167416377 1105 70-71 0.6212862947018536 1105 72-73 0.563531989285103 1105 74-75 0.5464262410656389 1105 76-77 0.5404505955067123 1105 78-79 0.7438296055212312 1105 80-81 0.6134652012540265 1105 82-83 0.6621442677687135 1105 84-85 0.6665785965964268 1105 86-87 0.7288335759243267 1105 88-89 0.6530415069443514 1105 90-91 0.6483622946646435 1105 92-93 0.7102851067238483 1105 94-95 0.8575127609070989 1105 96-97 0.7886046096808279 1105 98-99 0.7546116202271627 1105 100 0.997509374156202 1106 1 0.22206972998922225 1106 2 0.20011991750573088 1106 3 0.17964654505816213 1106 4 0.09954313326578301 1106 5 0.08245418314242414 1106 6 0.10749822656645591 1106 7 0.13909817898526455 1106 8 0.12205386682042985 1106 9 0.06104472337270295 1106 10-11 0.1263233489024742 1106 12-13 0.15517238243945997 1106 14-15 0.19882944985054962 1106 16-17 0.2063457780214577 1106 18-19 0.21742575531255426 1106 20-21 0.18818165241984275 1106 22-23 0.23436888154687807 1106 24-25 0.23572370680442134 1106 26-27 0.22300635861635953 1106 28-29 0.23221426512736798 1106 30-31 0.2458120147821461 1106 32-33 0.3448046847826234 1106 34-35 0.3899866162043608 1106 36-37 0.44013117847472216 1106 38-39 0.4039905223467528 1106 40-41 0.3886912496133874 1106 42-43 0.3903257301837755 1106 44-45 0.4397550977001501 1106 46-47 0.37343030423145507 1106 48-49 0.4167385842748672 1106 50-51 0.3180983216918065 1106 52-53 0.44821445650079994 1106 54-55 0.37921125001915357 1106 56-57 0.441376454564967 1106 58-59 0.5617278900435352 1106 60-61 0.5980577645013199 1106 62-63 0.47604067109889314 1106 64-65 0.5348156726612316 1106 66-67 0.5358955129039131 1106 68-69 0.4795836883946336 1106 70-71 0.5764877276653309 1106 72-73 0.5623823070651284 1106 74-75 0.46740316238949475 1106 76-77 0.47855338572956185 1106 78-79 0.5702281748219811 1106 80-81 0.6580993038355523 1106 82-83 0.6607771762140011 1106 84-85 0.5947204275921862 1106 86-87 0.5518189386493582 1106 88-89 0.6375445006527336 1106 90-91 0.59333186043418 1106 92-93 0.7979387237851299 1106 94-95 0.8475253138581316 1106 96-97 0.7730022981461424 1106 98-99 0.7575858242265845 1106 100 0.7692404016154306 1107 1 0.25286458559258307 1107 2 0.2230013456256117 1107 3 0.3017381439844513 1107 4 0.19435713146500433 1107 5 0.1826180694441959 1107 6 0.1777252316217286 1107 7 0.1853587483493655 1107 8 0.18818864218425801 1107 9 0.21158464003566735 1107 10-11 0.22506642966927615 1107 12-13 0.21948939613255192 1107 14-15 0.31475216915231385 1107 16-17 0.29234622364013774 1107 18-19 0.1969646068553672 1107 20-21 0.26748743412863973 1107 22-23 0.34375084433877845 1107 24-25 0.3066949056172348 1107 26-27 0.3333680841251123 1107 28-29 0.3810633123270435 1107 30-31 0.23282897081936937 1107 32-33 0.23295897162729062 1107 34-35 0.3015856903312297 1107 36-37 0.37294465854542835 1107 38-39 0.3465248958793907 1107 40-41 0.4100951138946911 1107 42-43 0.44291630235675683 1107 44-45 0.519969628248262 1107 46-47 0.3627429356555254 1107 48-49 0.551193637682232 1107 50-51 0.45140606538996764 1107 52-53 0.3975889535958359 1107 54-55 0.37735336154772625 1107 56-57 0.48292133287164063 1107 58-59 0.6062871182573204 1107 60-61 0.6155724090236205 1107 62-63 0.5480229596922541 1107 64-65 0.5833648776225928 1107 66-67 0.6286811717261358 1107 68-69 0.5319086845051473 1107 70-71 0.5603562258036519 1107 72-73 0.603410816805031 1107 74-75 0.5512584081239069 1107 76-77 0.49833889038039914 1107 78-79 0.6597171001769802 1107 80-81 0.571149894579488 1107 82-83 0.6466249111968985 1107 84-85 0.6156008835171178 1107 86-87 0.4360056000784205 1107 88-89 0.48226284571083866 1107 90-91 0.3894913208202304 1107 92-93 0.4692410918999492 1107 94-95 0.7714632032024937 1107 96-97 0.820311708162496 1107 98-99 0.7826549549604032 1107 100 0.8528635982853281 1108 1 0.2558675211967696 1108 2 0.1394262879915047 1108 3 0.1565116009899441 1108 4 0.10469028139392123 1108 5 0.10010274474696246 1108 6 0.12082469145151009 1108 7 0.1736875169757397 1108 8 0.12398457087825676 1108 9 0.14004025469323977 1108 10-11 0.10576826101565473 1108 12-13 0.1523768402299126 1108 14-15 0.16827951329992885 1108 16-17 0.20776972461508336 1108 18-19 0.1994777461155408 1108 20-21 0.1571438047570055 1108 22-23 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88-89 -0.25766415905663465 2316 90-91 -0.08283884535964248 2316 92-93 -0.05292910193271183 2316 94-95 -0.0010369821860223283 2316 96-97 -0.04677722569806164 2316 98-99 -0.2222485712487483 2316 100 -0.4818518961278997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 5.0 11 17.0 12 42.0 13 142.0 14 442.0 15 1159.0 16 2520.0 17 5416.0 18 9778.0 19 14996.0 20 20206.0 21 27134.0 22 34684.0 23 39219.0 24 41340.0 25 44781.0 26 51323.0 27 61138.0 28 72972.0 29 87188.0 30 104805.0 31 127352.0 32 155267.0 33 194575.0 34 257239.0 35 346125.0 36 498136.0 37 757961.0 38 716631.0 39 104945.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.296052352916256 20.134959125757128 14.121044940488797 23.447943580837816 2 15.299760877909458 21.34239491356294 39.87964161619726 23.478202592330344 3 17.25254332857714 27.308849962806388 31.10844917181333 24.33015753680315 4 12.135369236959699 17.683797835617018 37.84707956542917 32.33375336199411 5 12.395370144617461 38.30804797797899 35.21275939560813 14.08382248179542 6 30.117883814648344 37.76345567331907 18.33744404085947 13.781216471173115 7 26.3378505444796 33.33113614319538 22.95288059568737 17.378132716637655 8 25.826991645570356 36.04714419551255 20.487161275515106 17.63870288340198 9 26.864165097527525 15.209835795644214 20.59446482361976 37.331534283208505 10-11 24.60484991338306 26.354739789154458 29.101240697679344 19.939169599783142 12-13 25.028364930515846 24.390319444973887 28.95823803352012 21.623077590990146 14-15 22.629973061919465 25.593029559884283 26.739023049848154 25.037974328348096 16-17 21.903080152909705 28.404429515792557 27.47227118507668 22.220219146221062 18-19 21.80002509586993 27.36503425268576 29.578037301781013 21.256903349663297 20-21 22.562086748513178 26.702963769212268 29.523580343441907 21.211369138832644 22-23 22.338276216978613 26.496882107777736 29.41531976263993 21.74952191260372 24-25 22.005791582959436 26.58569004098158 29.487848079350883 21.920670296708106 26-27 21.88674125655684 26.66210462085826 29.45240890578405 21.99874521680086 28-29 22.000839176019156 27.00639176813328 29.405892498110532 21.586876557737032 30-31 22.532793309199995 26.68658820794944 29.4780747669316 21.30254371591896 32-33 22.25458977502124 26.75026019567986 29.376850043236892 21.61829998606201 34-35 22.365571870277424 26.969082830661677 29.208096734274463 21.457248564786436 36-37 22.402120339371837 26.963552828537647 29.179219583490017 21.4551072486005 38-39 22.328611152584838 27.07723801929958 29.09439649375829 21.499754334357295 40-41 22.34350195527003 26.842257764724554 29.213599302474975 21.600640977530443 42-43 22.1683009395293 27.053435017500515 28.852569164833135 21.925694878137048 44-45 21.94500049836374 27.247630389625137 28.998640982601216 21.808728129409907 46-47 22.018741726053324 27.003951374178364 28.69255460291873 22.284752296849582 48-49 22.009429658922105 27.206517155027054 28.664675260792894 22.119377925257947 50-51 22.12376295683662 27.396060046811797 28.48854710023395 21.99162989611763 52-53 22.234682290109294 27.502233348039212 28.16143006281701 22.101654299034482 54-55 22.05874586314216 27.317126826402138 28.521119070426547 22.10300824002915 56-57 22.160324450391997 27.399777363919988 28.14216677057233 22.297731415115692 58-59 22.14835067670383 27.382930925486864 28.401812026595874 22.06690637121343 60-61 22.48931765353722 27.452399358264984 28.331369828923613 21.726913159274183 62-63 22.313643701570463 27.495527289984505 28.357914760704222 21.83291424774081 64-65 22.16072810610109 27.20966830107297 28.35219521066846 22.27740838215749 66-67 22.0492823312958 27.24775564304257 28.272166479071576 22.43079554659006 68-69 21.872524568983774 27.35614701147965 28.37466111746516 22.396667302071418 70-71 21.985322742283888 27.245889005770064 28.340124487056435 22.42866376488961 72-73 21.895332004693305 27.687451552645065 27.88883900017367 22.528377442487958 74-75 21.945740887322863 27.617665979250443 27.87667805889189 22.559915074534803 76-77 21.886300254551276 27.413190682610654 28.05177274558122 22.648736317256844 78-79 21.813377238093047 27.33065997187914 28.165086026484225 22.69087676354359 80-81 21.86839688145172 27.577832993080175 27.785254390364422 22.76851573510368 82-83 22.122890754433612 27.52434655004769 27.84143914206418 22.511323553454517 84-85 21.94279738146773 27.6931763273033 27.66659385611311 22.697432435115868 86-87 21.74045720274236 27.664136536476214 27.847535009607473 22.747871251173958 88-89 21.628087854972176 27.465753016613963 27.951323775399363 22.954835353014495 90-91 22.018032464154775 27.360172807474058 27.83554958265687 22.786245145714297 92-93 21.83905184828969 27.470188865310647 27.717268326759243 22.973490959640422 94-95 21.660019943479792 27.349088885396228 27.70135042860209 23.28954074252189 96-97 21.6199148709249 27.28029272899836 27.734441556735966 23.365350843340764 98-99 21.501491236791246 27.221025399544097 27.659467152744504 23.61801621092015 100 20.703061600299293 27.723272192054765 27.947610740184825 23.626055467461114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2026.0 1 1507.5 2 1927.5 3 2670.0 4 1855.5 5 1429.0 6 2117.0 7 4045.5 8 6256.5 9 7316.5 10 6349.5 11 4794.5 12 4734.0 13 5111.5 14 5440.0 15 5711.0 16 5997.5 17 6449.0 18 6870.0 19 7274.5 20 7796.5 21 8727.5 22 10157.5 23 12222.0 24 14945.5 25 18159.5 26 22404.0 27 27091.5 28 32141.5 29 38389.5 30 45476.5 31 52455.0 32 60095.5 33 68519.0 34 77088.0 35 84589.5 36 94148.0 37 103450.0 38 109506.0 39 115245.5 40 117647.5 41 119159.5 42 121372.0 43 123692.0 44 126800.0 45 130922.5 46 133873.5 47 135305.5 48 138144.5 49 140954.5 50 142557.0 51 141151.5 52 135603.5 53 129041.0 54 122667.5 55 113726.0 56 103285.5 57 92923.0 58 82417.0 59 70416.0 60 57463.0 61 46522.0 62 37689.0 63 29711.0 64 22373.5 65 16817.5 66 12756.5 67 9755.5 68 7500.5 69 5760.5 70 4504.0 71 3449.5 72 2645.0 73 2028.5 74 1544.0 75 1154.0 76 827.5 77 574.0 78 400.0 79 280.5 80 189.0 81 126.5 82 89.0 83 66.5 84 46.5 85 38.0 86 28.0 87 20.5 88 16.0 89 9.0 90 5.0 91 2.5 92 3.0 93 4.0 94 4.0 95 2.5 96 2.0 97 2.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0029648830777180566 2 0.06599512064956352 3 5.823877474089039E-4 4 8.471094507765875E-4 5 0.001826579753237017 6 5.029712363985989E-4 7 0.0 8 9.265259617868927E-4 9 7.941651101030509E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.544568545978983E-4 18-19 0.0010059424727971978 20-21 0.003057535673896746 22-23 0.0 24-25 2.647217033676836E-5 26-27 1.8530519235737854E-4 28-29 9.794703024604294E-4 30-31 0.001760399327395096 32-33 0.0028192861408658306 34-35 0.004037005976357175 36-37 0.0011515394096494238 38-39 0.0015883302202061016 40-41 0.0052017814711749835 42-43 0.006220960029140565 44-45 0.005042948449154373 46-47 0.006843056032054622 48-49 0.008497566678102645 50-51 0.00791517893069374 52-53 0.004010533806020407 54-55 0.002051593201099548 56-57 0.0023030788192988476 58-59 0.003282549121759277 60-61 0.007729873738336362 62-63 0.01269340567648043 64-65 0.0035869790806321127 66-67 0.009437328725057922 68-69 0.011435977585483932 70-71 0.005598864026226509 72-73 0.0067768756062127 74-75 0.0032296047810857404 76-77 0.003189896525580588 78-79 0.007544568545978983 80-81 0.005757697048247119 82-83 0.015340622710157266 84-85 0.016598050801153764 86-87 0.021257152780424997 88-89 0.02010561337077557 90-91 0.02285871908579948 92-93 0.03273283862141408 94-95 0.036544831149908724 96-97 0.021614527079971368 98-99 0.017299563315078124 100 0.01776282629597157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3777552.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.68041035784344 #Duplication Level Percentage of deduplicated Percentage of total 1 77.28096655319176 49.21283662958025 2 14.502090171093313 18.4699810628334 3 4.065034238998417 7.765891453743093 4 1.5817480527673602 4.029054603317812 5 0.7942952597851204 2.529052404420317 6 0.4610641921429232 1.7616454174181357 7 0.28782897887708264 1.2830347241440172 8 0.19988903997445223 1.0183212873286793 9 0.14739707513969946 0.8447675607397752 >10 0.6228387818415759 6.797638429538154 >50 0.03594624955676989 1.581078317616837 >100 0.019137576317423247 2.2832186712965163 >500 0.0012094836452979385 0.5021089207978328 >1k 4.031612136686159E-4 0.5591040261745313 >5k 0.0 0.0 >10k+ 1.51185455125731E-4 1.3622664910505498 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16377 0.43353473360525546 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15408 0.4078832005489269 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10664 0.2822992244712978 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3935 0.1041679902751835 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.6472170336768362E-5 0.0 5 0.0 0.0 0.0 2.6472170336768362E-5 0.0 6 0.0 0.0 0.0 2.6472170336768362E-5 0.0 7 0.0 0.0 0.0 2.6472170336768362E-5 0.0 8 0.0 0.0 0.0 2.6472170336768362E-5 0.0 9 0.0 0.0 0.0 2.6472170336768362E-5 0.0 10-11 0.0 0.0 0.0 5.2944340673536724E-5 0.0 12-13 0.0 0.0 0.0 5.2944340673536724E-5 0.0 14-15 0.0 1.3236085168384181E-5 0.0 5.2944340673536724E-5 6.618042584192091E-5 16-17 0.0 2.6472170336768362E-5 0.0 9.265259617868927E-5 1.0588868134707345E-4 18-19 0.0 2.6472170336768362E-5 0.0 1.0588868134707345E-4 1.0588868134707345E-4 20-21 0.0 2.6472170336768362E-5 0.0 1.45596936852226E-4 1.0588868134707345E-4 22-23 0.0 2.6472170336768362E-5 0.0 2.2501344786253106E-4 1.3236085168384182E-4 24-25 2.6472170336768362E-5 2.6472170336768362E-5 0.0 4.1031864021990965E-4 1.7206910718899434E-4 26-27 2.6472170336768362E-5 2.6472170336768362E-5 0.0 6.220960029140565E-4 1.8530519235737854E-4 28-29 2.6472170336768362E-5 2.6472170336768362E-5 0.0 0.0015750941350377175 1.8530519235737854E-4 30-31 2.6472170336768362E-5 2.6472170336768362E-5 0.0 0.004606157638597695 2.117773626941469E-4 32-33 2.6472170336768362E-5 2.6472170336768362E-5 0.0 0.012587516995133356 2.6472170336768364E-4 34-35 2.6472170336768362E-5 2.6472170336768362E-5 0.0 0.02427498019881659 2.9119387370445197E-4 36-37 2.6472170336768362E-5 3.9708255505152544E-5 0.0 0.044115871866224475 2.9119387370445197E-4 38-39 2.6472170336768362E-5 6.618042584192091E-5 0.0 0.07657075269910249 2.9119387370445197E-4 40-41 2.6472170336768362E-5 7.941651101030509E-5 0.0 0.11600105041571897 2.9119387370445197E-4 42-43 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.15365771271977197 2.9119387370445197E-4 44-45 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.19974576127608568 3.309021292096045E-4 46-47 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.25585352630486624 3.7061038471475707E-4 48-49 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.31868522259918597 4.235547253882938E-4 50-51 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.386731936449849 4.235547253882938E-4 52-53 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.46017897304921285 4.36790810556678E-4 54-55 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.5435001291841912 4.500268957250622E-4 56-57 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.6395014549104817 4.500268957250622E-4 58-59 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.749308017467397 4.500268957250622E-4 60-61 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.8677577436392669 4.897351512302147E-4 62-63 2.6472170336768362E-5 1.0588868134707345E-4 0.0 0.9975375587152737 5.294434067353673E-4 64-65 2.6472170336768362E-5 1.0588868134707345E-4 0.0 1.130361673380009 5.294434067353673E-4 66-67 2.6472170336768362E-5 1.0588868134707345E-4 0.0 1.2835825952892244 5.294434067353673E-4 68-69 2.6472170336768362E-5 1.0588868134707345E-4 0.0 1.4615682325484864 5.294434067353673E-4 70-71 2.6472170336768362E-5 1.1912476651545763E-4 2.6472170336768362E-5 1.6550003811992529 5.294434067353673E-4 72-73 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 1.8486045989572082 6.353320880824407E-4 74-75 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 2.0550742914988334 6.353320880824407E-4 76-77 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 2.284945382618161 6.485681732508249E-4 78-79 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 2.549971515944718 6.618042584192091E-4 80-81 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 2.8287764139315623 7.147485990927458E-4 82-83 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 3.11631712812954 7.941651101030509E-4 84-85 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 3.4110450365739506 7.941651101030509E-4 86-87 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 3.7258520862188 8.07401195271435E-4 88 2.6472170336768362E-5 1.3236085168384182E-4 2.6472170336768362E-5 3.9724403529057972 8.471094507765876E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7415 0.0 52.636257 1 GTATCAA 12795 0.0 37.817528 1 TACCTGG 3185 0.0 33.21891 2 GTACCTG 3590 0.0 30.650635 1 TCAACGC 16430 0.0 29.08608 4 ATCAACG 16595 0.0 28.740252 3 CAACGCA 16685 0.0 28.64155 5 TATCAAC 17065 0.0 28.299349 2 AACGCAG 17275 0.0 27.744211 6 GTACATG 13870 0.0 26.919197 1 TACATGG 14045 0.0 25.679462 2 ACCTGGG 4190 0.0 24.672344 3 ACATGGG 14055 0.0 24.472706 3 TATAACG 250 1.6370905E-11 24.45207 2 ACGCAGA 19580 0.0 24.310116 7 CGCAGAG 20045 0.0 23.722733 8 CATGGGG 9345 0.0 23.532513 4 ATGGGGG 6055 0.0 22.039715 5 GCAGAGT 21805 0.0 21.269209 9 GAGTACT 14135 0.0 20.32917 12-13 >>END_MODULE