FastQCFastQC Report
Mon 6 Jun 2016
ERR1211556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4973157
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256210.5151858266288395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224970.45236858599074997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145100.2917663769714087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144700.043.217611
GTATCAA237450.034.586411
GTACATG277200.028.675461
TACCTGG35700.028.4485862
ATCAACG280800.028.4165293
TCAACGC283150.028.2130284
CAACGCA283900.028.2032835
TACATGG280500.027.6583442
AACGCAG291000.027.6379496
TATCAAC292250.027.4955652
ACATGGG273100.027.3077323
CATGGGG91600.025.2398624
TAACGCA4750.024.7322544
ACGCAGA327850.024.288617
CGCAGAG328800.024.0905678
GTACCTG44900.023.7024541
GAGTACT203600.022.29345512-13
GCAGAGT354100.021.8136189
CATGGGA156050.021.7114494
ACCTGGG46950.020.818923
Produced by FastQC (version 0.11.5)