##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211556_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4973157
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.582393638487584	31.0	31.0	34.0	30.0	34.0
2	31.608452739376617	33.0	31.0	34.0	30.0	34.0
3	31.707683268394703	33.0	31.0	34.0	28.0	34.0
4	35.26365284667265	37.0	35.0	37.0	33.0	37.0
5	35.368299050281344	37.0	35.0	37.0	33.0	37.0
6	35.482056367816256	37.0	35.0	37.0	33.0	37.0
7	35.424620417171624	37.0	35.0	37.0	33.0	37.0
8	35.39631204082236	37.0	35.0	37.0	33.0	37.0
9	36.99922142011604	39.0	37.0	39.0	33.0	39.0
10-11	36.98599642038246	39.0	37.0	39.0	33.0	39.0
12-13	36.94650148788787	39.0	37.0	39.0	33.0	39.0
14-15	38.157194715549906	40.0	38.0	41.0	33.0	41.0
16-17	38.15312707803112	40.0	38.0	41.0	33.0	41.0
18-19	38.20855484755458	40.0	38.0	41.0	33.0	41.0
20-21	38.18586925769687	40.0	38.0	41.0	33.0	41.0
22-23	38.05141864614369	40.0	38.0	41.0	33.0	41.0
24-25	37.981239984983375	40.0	38.0	41.0	33.0	41.0
26-27	37.84307824989237	40.0	37.5	41.0	32.0	41.0
28-29	37.69653129792604	40.0	37.0	41.0	32.0	41.0
30-31	37.513429598140576	40.0	37.0	41.0	32.0	41.0
32-33	37.32520801173178	40.0	36.5	41.0	31.0	41.0
34-35	37.16192269819754	40.0	36.0	41.0	31.0	41.0
36-37	36.9916299646281	39.5	36.0	41.0	30.0	41.0
38-39	36.86428278857876	39.0	36.0	41.0	30.0	41.0
40-41	36.835677618864636	39.0	36.0	41.0	30.0	41.0
42-43	36.76695879498676	39.0	36.0	41.0	30.0	41.0
44-45	36.56850417149509	39.0	35.0	41.0	30.0	41.0
46-47	36.470110434880695	39.0	35.0	41.0	29.0	41.0
48-49	36.39205126642895	39.5	36.0	41.0	28.0	41.0
50-51	35.41640018603877	38.5	34.0	40.0	26.5	40.5
52-53	35.61483631021502	38.5	35.0	40.0	27.5	40.5
54-55	36.21716708320288	39.0	35.0	41.0	28.0	41.0
56-57	36.20565719119666	39.0	35.0	41.0	28.0	41.0
58-59	36.082314614238	39.0	35.0	41.0	27.5	41.0
60-61	35.925850420567855	39.0	35.0	41.0	27.0	41.0
62-63	35.70148157397806	39.0	35.0	41.0	26.5	41.0
64-65	35.459928773614024	38.5	34.5	40.0	26.0	41.0
66-67	35.18328568754214	38.0	34.0	40.0	26.0	41.0
68-69	34.91380264085771	37.0	34.0	40.0	26.0	41.0
70-71	34.580094595847264	37.0	34.0	39.5	26.0	41.0
72-73	34.233175123970554	36.0	34.0	39.0	25.5	41.0
74-75	33.857161859157074	36.0	33.0	39.0	25.0	40.5
76-77	33.49287535060727	35.5	33.0	38.5	25.0	40.0
78-79	33.14414616309117	35.0	33.0	37.0	24.0	39.0
80-81	32.77059300560992	35.0	32.5	37.0	23.5	39.0
82-83	32.43418426162697	35.0	32.0	36.0	23.0	39.0
84-85	32.127345165254184	35.0	32.0	36.0	22.5	37.0
86-87	31.814711057784823	35.0	32.0	36.0	21.0	37.0
88-89	31.556714778962338	35.0	31.5	35.0	20.0	36.5
90-91	31.30343904284542	35.0	31.0	35.0	20.0	36.0
92-93	31.127523321704906	34.5	31.0	35.0	20.0	36.0
94-95	30.947525686400006	34.0	31.0	35.0	19.0	36.0
96-97	30.832507097604196	34.0	31.0	35.0	19.0	35.5
98-99	30.658550795802345	34.0	31.0	35.0	18.5	35.0
100	29.314441711773828	33.0	28.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.45474519232623223
1101	2	0.40500191467278057
1101	3	0.33687027466777764
1101	4	0.3606266544812158
1101	5	0.3403139687473882
1101	6	0.3758979670203004
1101	7	0.4157736553676088
1101	8	0.4086737615249092
1101	9	0.45523511957502194
1101	10-11	0.4282140755064816
1101	12-13	0.46307451572707237
1101	14-15	0.5048628466178826
1101	16-17	0.516711942139132
1101	18-19	0.43236048013486794
1101	20-21	0.4668471087155268
1101	22-23	0.5450978681624719
1101	24-25	0.5226740399248442
1101	26-27	0.43795812454484206
1101	28-29	0.5620856229365359
1101	30-31	0.5608969277377156
1101	32-33	0.5241427104039431
1101	34-35	0.39910215553260997
1101	36-37	0.4849637607950896
1101	38-39	0.4933906315499783
1101	40-41	0.44455796323980223
1101	42-43	0.3861676303817987
1101	44-45	0.33991424916097657
1101	46-47	0.32943751324891934
1101	48-49	0.4385932626772373
1101	50-51	0.42398654524694734
1101	52-53	0.49306497133239446
1101	54-55	0.5007855967260539
1101	56-57	0.48779063084629826
1101	58-59	0.4631011145741226
1101	60-61	0.43022206431149357
1101	62-63	0.4265635486183541
1101	64-65	0.48654035637502346
1101	66-67	0.45201592084616493
1101	68-69	0.3718296500410929
1101	70-71	0.3876500942865917
1101	72-73	0.37381057616713065
1101	74-75	0.28861592988308615
1101	76-77	0.2805150038267996
1101	78-79	0.2415336546882756
1101	80-81	0.20882014845939523
1101	82-83	0.18111607702837773
1101	84-85	0.13778723939933712
1101	86-87	0.05733620294462938
1101	88-89	0.08048763493415478
1101	90-91	0.021940412622317496
1101	92-93	-0.027105017193395753
1101	94-95	-0.12362749673173923
1101	96-97	-0.09916291071576921
1101	98-99	-0.1994931298940017
1101	100	-0.1655091790720462
1102	1	0.33836314804620216
1102	2	0.37194414079360527
1102	3	0.39181383972110595
1102	4	0.29672864753252526
1102	5	0.3189018315623713
1102	6	0.336059631212855
1102	7	0.2958722825648721
1102	8	0.3222403099103843
1102	9	0.3749673727655747
1102	10-11	0.3405243989142477
1102	12-13	0.3732735066323869
1102	14-15	0.42467764195438207
1102	16-17	0.42851945471478103
1102	18-19	0.43293415714518346
1102	20-21	0.4096610632500344
1102	22-23	0.41577633781025725
1102	24-25	0.3963103662750882
1102	26-27	0.37877299132635045
1102	28-29	0.3569767585947474
1102	30-31	0.31790373221387824
1102	32-33	0.29537519314467886
1102	34-35	0.37585364992417425
1102	36-37	0.3568811233891296
1102	38-39	0.28766644701544664
1102	40-41	0.29725868404062794
1102	42-43	0.31084146404192126
1102	44-45	0.2841427653803166
1102	46-47	0.2960752380691858
1102	48-49	0.38081197439130676
1102	50-51	0.41931180860121486
1102	52-53	0.40049275563724507
1102	54-55	0.45648905889856195
1102	56-57	0.5579312948350932
1102	58-59	0.5108189259486906
1102	60-61	0.4312448691634003
1102	62-63	0.5074545870319511
1102	64-65	0.48573946106313315
1102	66-67	0.40969321694341687
1102	68-69	0.36562440240473393
1102	70-71	0.4178113681776736
1102	72-73	0.43366383604440273
1102	74-75	0.3797135833112506
1102	76-77	0.38540430705268136
1102	78-79	0.34239387251619036
1102	80-81	0.3630410438771179
1102	82-83	0.332022833345313
1102	84-85	0.24278561204066307
1102	86-87	0.30312769463716194
1102	88-89	0.3686108273091193
1102	90-91	0.1951501857711655
1102	92-93	0.21146245733116587
1102	94-95	0.20349356023110587
1102	96-97	0.01612804249304034
1102	98-99	0.03266800904538414
1102	100	0.11769879472086586
1103	1	0.22778107637047995
1103	2	0.266028636626352
1103	3	0.20398139881156752
1103	4	0.19842966729631684
1103	5	0.22722968510049668
1103	6	0.21905778373903928
1103	7	0.22398278394004478
1103	8	0.21384382846079575
1103	9	0.21411829154457251
1103	10-11	0.27041403975281497
1103	12-13	0.20284714594704667
1103	14-15	0.25839929053772437
1103	16-17	0.2731546699663525
1103	18-19	0.25331012328526725
1103	20-21	0.2993924489075823
1103	22-23	0.35210131315117366
1103	24-25	0.314796977308788
1103	26-27	0.3302061208634868
1103	28-29	0.2997229775360495
1103	30-31	0.23211512444718352
1103	32-33	0.29122930403485725
1103	34-35	0.3308314567535646
1103	36-37	0.3240269785764056
1103	38-39	0.3246770817322968
1103	40-41	0.18874993202484802
1103	42-43	0.18680101532498128
1103	44-45	0.1947784933841632
1103	46-47	0.14278468627990293
1103	48-49	0.2046015920059716
1103	50-51	0.269572588141223
1103	52-53	0.20695483675908832
1103	54-55	0.29883267598091834
1103	56-57	0.21753323337788544
1103	58-59	0.2951391116876536
1103	60-61	0.23448939719646944
1103	62-63	0.24697761000012264
1103	64-65	0.2790535564011307
1103	66-67	0.3116547226867681
1103	68-69	0.22967156387081644
1103	70-71	0.33202602219384403
1103	72-73	0.26814623024521467
1103	74-75	0.16904422632607918
1103	76-77	0.1264427217218298
1103	78-79	0.18254883329651506
1103	80-81	0.10661034110643186
1103	82-83	0.16805366110752118
1103	84-85	0.06513065126033979
1103	86-87	0.13434843644486705
1103	88-89	0.22649755733265664
1103	90-91	-0.00244979439719728
1103	92-93	-0.08651445446500716
1103	94-95	-0.0611142690915365
1103	96-97	-0.004308723279638826
1103	98-99	-0.036703393208117774
1103	100	0.03683280441749304
1104	1	0.4131119606147067
1104	2	0.41029767061132816
1104	3	0.4223709176247361
1104	4	0.3803842112072715
1104	5	0.29281994953419854
1104	6	0.3140332844422602
1104	7	0.3511106822725836
1104	8	0.3830499961037006
1104	9	0.48700508640175144
1104	10-11	0.47766030400092774
1104	12-13	0.41924714971280963
1104	14-15	0.48260120993229805
1104	16-17	0.4669186549745987
1104	18-19	0.4125752889602339
1104	20-21	0.3761836142994355
1104	22-23	0.46758843946250295
1104	24-25	0.4526605875230132
1104	26-27	0.4756240143442554
1104	28-29	0.47755915822126127
1104	30-31	0.47397028927148455
1104	32-33	0.4629032550940906
1104	34-35	0.41686418457282315
1104	36-37	0.4705454659377395
1104	38-39	0.45479768356803874
1104	40-41	0.40849615004151474
1104	42-43	0.3510370350239427
1104	44-45	0.47702908657546317
1104	46-47	0.49898343033159875
1104	48-49	0.5268599588024614
1104	50-51	0.5548147416584968
1104	52-53	0.5156165225353035
1104	54-55	0.5895965772682317
1104	56-57	0.6080888578603023
1104	58-59	0.6082053080377605
1104	60-61	0.6574198681485015
1104	62-63	0.5840114416578643
1104	64-65	0.6278443102491877
1104	66-67	0.5153116962191859
1104	68-69	0.3603162284105039
1104	70-71	0.36418665742380796
1104	72-73	0.4619825548362684
1104	74-75	0.47638522298161234
1104	76-77	0.503062276037376
1104	78-79	0.4464093192746006
1104	80-81	0.33175450752345625
1104	82-83	0.40263847090468374
1104	84-85	0.318170258652934
1104	86-87	0.320961494823905
1104	88-89	0.40632446348823237
1104	90-91	0.3602163571672605
1104	92-93	0.37275219061578113
1104	94-95	0.36763515889955656
1104	96-97	0.375761461077861
1104	98-99	0.21455764895932106
1104	100	0.3814841639410176
1105	1	0.2978248238890373
1105	2	0.2936990198785985
1105	3	0.24597391392954648
1105	4	0.24895007288858295
1105	5	0.20289061506071704
1105	6	0.16231579416243846
1105	7	0.26102611324923686
1105	8	0.22222998838487484
1105	9	0.26555900260770215
1105	10-11	0.2313183805991983
1105	12-13	0.25574154652808545
1105	14-15	0.3507632833139951
1105	16-17	0.31632816821320375
1105	18-19	0.3375035963733737
1105	20-21	0.42001068994376567
1105	22-23	0.4081243916366759
1105	24-25	0.4269238111608047
1105	26-27	0.34768004499029104
1105	28-29	0.37305032043999375
1105	30-31	0.403823406772851
1105	32-33	0.3950247773536759
1105	34-35	0.3833793405341268
1105	36-37	0.3905431973098956
1105	38-39	0.4026241853627752
1105	40-41	0.4919214897908475
1105	42-43	0.5314120313129607
1105	44-45	0.4006627831466645
1105	46-47	0.39470104348112756
1105	48-49	0.4332197532256572
1105	50-51	0.4273822260436546
1105	52-53	0.4691293520376334
1105	54-55	0.5266170657920171
1105	56-57	0.5062706779141806
1105	58-59	0.4826448737310969
1105	60-61	0.5012450389691523
1105	62-63	0.5267603012948356
1105	64-65	0.534020743942186
1105	66-67	0.4898226155780492
1105	68-69	0.5078045982606696
1105	70-71	0.49669875434123867
1105	72-73	0.5066797058893044
1105	74-75	0.4997393738069249
1105	76-77	0.44293341042509127
1105	78-79	0.4011745274469476
1105	80-81	0.43100580885266027
1105	82-83	0.41035022680719635
1105	84-85	0.3419102134965968
1105	86-87	0.3602679262122379
1105	88-89	0.3640267381737594
1105	90-91	0.2613639705347275
1105	92-93	0.2995849785002811
1105	94-95	0.35130872634773525
1105	96-97	0.3507602898687061
1105	98-99	0.4032087437781833
1105	100	0.4628248371949404
1106	1	0.3731644973715369
1106	2	0.3112182537371311
1106	3	0.31618290518837355
1106	4	0.2869023107009383
1106	5	0.3365679984789196
1106	6	0.30899816194748553
1106	7	0.29256768486372664
1106	8	0.36310737637715107
1106	9	0.36725872708841223
1106	10-11	0.40629463879431427
1106	12-13	0.3833153607053532
1106	14-15	0.49665727713679075
1106	16-17	0.38765863512457344
1106	18-19	0.4090523480497339
1106	20-21	0.4057942466583313
1106	22-23	0.4380214652465355
1106	24-25	0.4881144642261148
1106	26-27	0.49312519022689827
1106	28-29	0.4705543448016414
1106	30-31	0.5148427626986347
1106	32-33	0.5468900607108935
1106	34-35	0.5069818358312261
1106	36-37	0.5338082848084156
1106	38-39	0.5443662221278913
1106	40-41	0.46639286694917104
1106	42-43	0.3878841926776033
1106	44-45	0.3546106564347937
1106	46-47	0.42080037550046967
1106	48-49	0.4998961822873582
1106	50-51	0.45770198851125343
1106	52-53	0.52562706348332
1106	54-55	0.49583188748427887
1106	56-57	0.5516313198225973
1106	58-59	0.5095289459386976
1106	60-61	0.4894813759100316
1106	62-63	0.5453481402553422
1106	64-65	0.5917770672600398
1106	66-67	0.6639598336350687
1106	68-69	0.5613046872623002
1106	70-71	0.4835972252491416
1106	72-73	0.4638549904671976
1106	74-75	0.48726605707437187
1106	76-77	0.5231323180471819
1106	78-79	0.4556060164442286
1106	80-81	0.4970958914533341
1106	82-83	0.43790739106645304
1106	84-85	0.4517436330301692
1106	86-87	0.5109425372158682
1106	88-89	0.5169741456499537
1106	90-91	0.4426451882699176
1106	92-93	0.4641486142527036
1106	94-95	0.5520817661281576
1106	96-97	0.4979059035625042
1106	98-99	0.4549006427285427
1106	100	0.5107772554904813
1107	1	0.21182892374065077
1107	2	0.2587744325043069
1107	3	0.2180175464129448
1107	4	0.2906093317497067
1107	5	0.2604836501353702
1107	6	0.2162267590981557
1107	7	0.23770330039238274
1107	8	0.21917885353521172
1107	9	0.20621735595731394
1107	10-11	0.3369003196707041
1107	12-13	0.2629823304307308
1107	14-15	0.3684836806913694
1107	16-17	0.3753058163586047
1107	18-19	0.23695092775412974
1107	20-21	0.25356464031656856
1107	22-23	0.3499575805995079
1107	24-25	0.2808028644127347
1107	26-27	0.3774549044289941
1107	28-29	0.3760398964681926
1107	30-31	0.3452309702000065
1107	32-33	0.34549330478379403
1107	34-35	0.3346694139200821
1107	36-37	0.46408109259639474
1107	38-39	0.49012420113616173
1107	40-41	0.47484618111598564
1107	42-43	0.4565691742729072
1107	44-45	0.5414404241914781
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2316	90-91	-0.16656259793908035
2316	92-93	-0.024501027177290524
2316	94-95	0.00846039048586178
2316	96-97	-0.054741358314213784
2316	98-99	0.09387553985173369
2316	100	-0.2027778713703654
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	21.0
1	45.0
2	55.0
3	66.0
4	88.0
5	81.0
6	104.0
7	358.0
8	1031.0
9	1884.0
10	2967.0
11	4426.0
12	6123.0
13	7658.0
14	9165.0
15	10782.0
16	13408.0
17	17196.0
18	22653.0
19	28957.0
20	35760.0
21	45560.0
22	53426.0
23	53047.0
24	49135.0
25	47184.0
26	49013.0
27	54483.0
28	62615.0
29	74343.0
30	90020.0
31	109628.0
32	138690.0
33	180987.0
34	248661.0
35	359156.0
36	554280.0
37	961999.0
38	1271160.0
39	405737.0
40	1205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.332563061941585	19.817104859236125	12.88716007544792	22.963172003374368
2	14.689275203269297	21.63341295758363	41.864306509146594	21.813005330000482
3	17.497639612828117	28.408783255109682	31.014240365657862	23.07933676640434
4	10.762909922252128	17.644923156266323	41.20415972136038	30.38800720012117
5	11.093735386372984	39.700873825755785	36.551782397374	12.653608390497235
6	27.541787945968498	39.64679445609061	19.773417587886648	13.038000010054244
7	25.02608605774867	33.95090612980631	24.382908137462515	16.6400996749825
8	24.51928171821683	36.2030138009376	22.134386556960564	17.143317923885007
9	26.58138732396632	14.893044558198495	21.73887251574128	36.78669560209391
10-11	23.05215664911763	27.323749030499766	30.436425418209772	19.187668902172828
12-13	24.09796547097218	25.659136829605618	30.155401237438472	20.087496461983733
14-15	21.52168546336617	26.66880854014228	28.445859622819096	23.363646373672452
16-17	19.96338837879655	29.49560181935988	29.305907530217763	21.235102271625813
18-19	19.749844639614185	28.48498987392178	31.6696364466415	20.095529039822537
20-21	20.89137190513464	27.663030230872508	31.710690171969812	19.734907692023036
22-23	20.368803822784418	27.913725422349238	31.247707876375504	20.469762878490844
24-25	19.96937925212051	28.01629500011614	31.72806016677307	20.28626558099028
26-27	19.992404514979917	28.106351269363007	31.56845056328656	20.33279365237052
28-29	19.821672407977616	28.029816199608153	31.807073850214234	20.341437542199994
30-31	20.3249515406294	27.952185480660546	31.647143986648874	20.07571899206118
32-33	19.87836466145377	28.2208506288116	31.567959338371654	20.332825371362972
34-35	20.215392403403406	28.31948733486594	31.594740649111564	19.87037961261909
36-37	20.252189882940428	28.156594567736764	31.326646657119444	20.264568892203368
38-39	19.973582936205858	28.55687612941244	31.356682652325496	20.112858282056205
40-41	20.26403218435932	28.27524570301312	31.470400590845752	19.990321521781805
42-43	20.066562845979558	28.243638803779707	31.156505059347563	20.533293290893173
44-45	20.10887319342593	28.58277371980863	31.01836988724049	20.289983199524954
46-47	20.16027880100524	28.548624402509297	30.675461523323367	20.615635273162095
48-49	20.09460184660729	28.694780689366134	30.670185737646943	20.54043172637963
50-51	20.270314703178403	28.811815690242547	30.355673906946993	20.562195699632053
52-53	20.283479788873937	28.955599348806405	30.170156456008883	20.590764406310775
54-55	20.2636106517497	28.980688987139263	30.185897562249114	20.569802798861918
56-57	20.31344069894205	29.012456356094248	29.982741041918963	20.69136190304474
58-59	20.318699537580557	28.901909192333786	30.229884271443492	20.549506998642165
60-61	20.46144450206869	29.23982959716026	30.00237033765294	20.29635556311811
62-63	20.15889160141662	29.049622894489623	30.304803776074664	20.486681728019096
64-65	20.093151482340772	28.834749600202507	30.23291692308249	20.839181994374222
66-67	20.161699051796226	28.957512844575113	29.93665903882797	20.944129064800695
68-69	20.194298054756686	29.066145030732866	30.01048213074776	20.729074783762684
70-71	20.190897521301387	28.862233915595887	30.0575436425344	20.88932492056833
72-73	20.227083958182337	28.979998998230005	29.85602473124704	20.936892312340618
74-75	20.2289207583151	29.126213689883784	29.700718294198374	20.944147257602737
76-77	20.092251761146215	28.926824088111815	29.963298810992494	21.017625339749472
78-79	20.180717803174282	28.87723038940635	29.76506223028282	21.176989577136553
80-81	20.160411776590042	29.167541982211848	29.53662764393481	21.135418597263303
82-83	20.385185548683864	29.021461699555513	29.63378975285449	20.95956299890613
84-85	20.28065827821855	29.038692453524035	29.529091766755837	21.15155750150158
86-87	20.080252872067533	29.055308675296654	29.794987428498104	21.069451024137706
88-89	20.399766588135353	28.765407903121403	29.71539580114057	21.11942970760267
90-91	20.545755460466022	28.910604004923364	29.604195095725284	20.939445438885333
92-93	20.48412392325419	28.862655732478792	29.54814262447662	21.1050777197904
94-95	20.294877009719457	28.67393334094224	29.663808633629596	21.367381015708705
96-97	20.506069560733618	28.652948063737526	29.615830450488062	21.225151925040795
98-99	20.416150009568064	28.537049329285146	29.591286232574483	21.455514428572307
100	20.51864346430177	28.455988630157243	29.51854187600394	21.50682602953705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2483.0
1	1711.5
2	1568.0
3	2294.0
4	1875.0
5	1735.5
6	2731.5
7	4925.5
8	7740.5
9	9057.0
10	7749.0
11	6364.5
12	6539.0
13	6946.5
14	7419.5
15	7959.0
16	8625.0
17	9272.5
18	10185.0
19	11424.5
20	13068.0
21	15601.5
22	18931.5
23	23286.0
24	28247.5
25	34415.0
26	42934.0
27	52325.0
28	61148.0
29	72256.5
30	86037.5
31	98895.0
32	113708.0
33	131912.5
34	152279.5
35	168714.5
36	182513.0
37	194289.0
38	199383.0
39	203876.0
40	200519.0
41	193115.0
42	187546.0
43	183581.0
44	182597.0
45	182300.5
46	178770.0
47	172820.5
48	170311.0
49	166297.5
50	161043.5
51	148287.0
52	129158.0
53	114600.5
54	102669.0
55	90033.0
56	77987.5
57	67162.0
58	56402.0
59	46014.0
60	36558.5
61	28556.5
62	22003.0
63	16529.0
64	12193.5
65	8733.0
66	6375.0
67	4871.5
68	3646.0
69	2818.5
70	2098.0
71	1478.0
72	1153.0
73	870.0
74	677.0
75	527.5
76	400.0
77	295.0
78	209.0
79	159.5
80	124.0
81	91.5
82	63.0
83	54.0
84	51.0
85	39.5
86	30.0
87	26.5
88	30.0
89	23.0
90	12.0
91	12.5
92	10.0
93	5.5
94	8.5
95	6.5
96	5.0
97	6.5
98	4.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17439626378173864
2	0.10536566611510556
3	0.030463546596256663
4	0.031649915737629035
5	0.02698487097833429
6	0.0026542496044263232
7	0.0039009426004447474
8	0.004705260662392118
9	0.004926448129427646
10-11	0.004705260662392118
12-13	0.0077315073704691
14-15	0.017584403629324393
16-17	0.008425231698898708
18-19	0.017031434961735575
20-21	0.017875968926780315
22-23	0.04685152710843434
24-25	0.014306807526888855
26-27	0.009370305421686868
28-29	0.02605990520709481
30-31	0.013924756447463856
32-33	0.020107951548684267
34-35	0.020560380458529662
36-37	0.020721244070919135
38-39	0.019947087936294794
40-41	0.02616044496483823
42-43	0.03549053448342773
44-45	0.037581761444490894
46-47	0.020278869136848083
48-49	0.012718279354542799
50-51	0.011712881777108585
52-53	0.02124405081118493
54-55	0.025718070030767176
56-57	0.0192332556563165
58-59	0.025416450757536912
60-61	0.02596941942512573
62-63	0.05225051209925606
64-65	0.03172029356804943
66-67	0.020490002628109266
68-69	0.02739708398508231
70-71	0.04143243416606393
72-73	0.03933115322922642
74-75	0.040306388879337615
76-77	0.02233993417058822
78-79	0.016468412318372413
80-81	0.022933118741274405
82-83	0.01817758820001058
84-85	0.017745267241713866
86-87	0.02007778962136124
88-89	0.016699653761182282
90-91	0.027175896518046787
92-93	0.02592920352202836
94-95	0.018388721691271762
96-97	0.012848981039609245
98-99	0.017845806999457287
100	0.04246799367082117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4973157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.10861410917114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89596479840705	45.767886111999225
2	15.964911975832132	19.511872904360217
3	4.998861092207517	9.164204204071769
4	1.8862373393952705	4.610613987656611
5	0.8553759837732653	2.6135420455326557
6	0.447016661776783	1.6389941210932388
7	0.24384511399987313	1.0430725881677567
8	0.14972017006881574	0.7319353677675791
9	0.09645403217432423	0.5304755008272921
>10	0.3872286334306697	4.185016971643805
>50	0.03485270419423864	1.507153691835106
>100	0.0346066509654199	4.2629259694261314
>500	0.0034430324133078653	1.3959303681632844
>1k	0.001351063299894917	1.3684186674738026
>5k	0.0	0.0
>10k+	1.3074806128015264E-4	1.6679574999814921
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25621	0.5151858266288395	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22497	0.45236858599074997	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14510	0.2917663769714087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.0107951548684268E-5	0.0
3	0.0	2.0107951548684268E-5	0.0	2.0107951548684268E-5	0.0
4	0.0	2.0107951548684268E-5	0.0	2.0107951548684268E-5	0.0
5	0.0	1.4075566084078986E-4	0.0	2.0107951548684268E-5	0.0
6	0.0	1.4075566084078986E-4	0.0	4.0215903097368535E-5	0.0
7	0.0	1.4075566084078986E-4	0.0	4.0215903097368535E-5	0.0
8	0.0	1.4075566084078986E-4	0.0	4.0215903097368535E-5	0.0
9	0.0	1.4075566084078986E-4	0.0	2.0107951548684267E-4	0.0
10-11	0.0	1.4075566084078986E-4	0.0	2.0107951548684267E-4	0.0
12-13	0.0	1.4075566084078986E-4	0.0	2.111334912611848E-4	0.0
14-15	0.0	1.4075566084078986E-4	0.0	2.815113216815797E-4	0.0
16-17	0.0	1.4075566084078986E-4	0.0	3.01619273230264E-4	0.0
18-19	0.0	1.4075566084078986E-4	0.0	3.217272247789483E-4	0.0
20-21	0.0	1.4075566084078986E-4	0.0	3.5188915210197466E-4	0.0
22-23	0.0	1.4075566084078986E-4	0.0	4.0215903097368534E-4	0.0
24-25	0.0	1.4075566084078986E-4	0.0	4.7253686139408027E-4	0.0
26-27	0.0	1.4075566084078986E-4	0.0	6.635624011065808E-4	0.0
28-29	0.0	1.6086361238947414E-4	0.0	0.002051011057965795	0.0
30-31	0.0	1.809715639381584E-4	0.0	0.007590751709628311	0.0
32-33	0.0	1.809715639381584E-4	0.0	0.018640071085630316	0.0
34-35	0.0	1.809715639381584E-4	0.0	0.03487724196119286	0.0
36-37	0.0	2.0107951548684267E-4	0.0	0.061781681133332406	0.0
38-39	0.0	2.2118746703552692E-4	0.0	0.10772835042207596	0.0
40-41	0.0	2.2118746703552692E-4	0.0	0.158068607124207	0.0
42-43	0.0	2.2118746703552692E-4	0.0	0.20644833855034134	0.0
44-45	0.0	2.3124144280986904E-4	0.0	0.2662996563350001	0.0
46-47	0.0	2.412954185842112E-4	0.0	0.33931162840827267	0.0
48-49	0.0	2.412954185842112E-4	0.0	0.4214827724119709	0.0
50-51	0.0	2.412954185842112E-4	0.0	0.5150752328953219	0.0
52-53	0.0	2.412954185842112E-4	0.0	0.6057620943798878	0.0
54-55	0.0	3.01619273230264E-4	0.0	0.7078903802956553	0.0
56-57	0.0	3.01619273230264E-4	0.0	0.8293323536739339	0.0
58-59	1.0053975774342134E-5	3.5188915210197466E-4	0.0	0.9667601485334165	0.0
60-61	2.0107951548684268E-5	3.619431278763168E-4	0.0	1.1196308501822887	0.0
62-63	2.0107951548684268E-5	3.619431278763168E-4	0.0	1.2797404143886872	0.0
64-65	2.0107951548684268E-5	4.1221300674802746E-4	0.0	1.4368237318870087	0.0
66-67	2.0107951548684268E-5	4.825908371684224E-4	0.0	1.614829372971736	0.0
68-69	2.0107951548684268E-5	4.825908371684224E-4	0.0	1.8237007196836938	0.0
70-71	2.0107951548684268E-5	5.127527644914488E-4	0.0	2.066192561385052	0.0
72-73	2.0107951548684268E-5	5.429146918144752E-4	0.0	2.315712132152675	0.0
74-75	2.0107951548684268E-5	5.429146918144752E-4	0.0	2.554514164744849	0.0
76-77	2.0107951548684268E-5	5.429146918144752E-4	0.0	2.809844933510042	0.0
78-79	2.0107951548684268E-5	5.429146918144752E-4	0.0	3.113877160926148	0.0
80-81	2.0107951548684268E-5	5.730766191375015E-4	0.0	3.4537618659535583	0.0
82-83	2.0107951548684268E-5	5.831305949118437E-4	0.0	3.8132719316924844	0.0
84-85	2.0107951548684268E-5	5.831305949118437E-4	0.0	4.157982545091579	0.0
86-87	2.0107951548684268E-5	5.831305949118437E-4	0.0	4.505377570022422	0.0
88	2.0107951548684268E-5	5.831305949118437E-4	0.0	4.789392331671813	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14470	0.0	43.21761	1
GTATCAA	23745	0.0	34.58641	1
GTACATG	27720	0.0	28.67546	1
TACCTGG	3570	0.0	28.448586	2
ATCAACG	28080	0.0	28.416529	3
TCAACGC	28315	0.0	28.213028	4
CAACGCA	28390	0.0	28.203283	5
TACATGG	28050	0.0	27.658344	2
AACGCAG	29100	0.0	27.637949	6
TATCAAC	29225	0.0	27.495565	2
ACATGGG	27310	0.0	27.307732	3
CATGGGG	9160	0.0	25.239862	4
TAACGCA	475	0.0	24.732254	4
ACGCAGA	32785	0.0	24.28861	7
CGCAGAG	32880	0.0	24.090567	8
GTACCTG	4490	0.0	23.702454	1
GAGTACT	20360	0.0	22.293455	12-13
GCAGAGT	35410	0.0	21.813618	9
CATGGGA	15605	0.0	21.711449	4
ACCTGGG	4695	0.0	20.81892	3
>>END_MODULE