FastQCFastQC Report
Mon 6 Jun 2016
ERR1211556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4973157
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273030.5490074011337266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241670.48594886507705265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174600.3510848340400273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150150.041.0623861
GTATCAA243200.032.5839081
GTACATG282100.028.1407811
TACCTGG35500.027.4226112
TACATGG285050.027.2226092
ACATGGG274450.027.1212233
ATCAACG294950.026.303653
TCAACGC297500.026.078194
CAACGCA299850.025.8738085
TAGATCG2950.025.4868015
AACGCAG308250.025.2449556
TATCAAC309200.025.202862
CATGGGG94300.024.965684
GTACCTG43000.023.295821
CATGGGA156050.022.8858574
ACGCAGA351300.022.030937
CGCAGAG352200.021.8812378
GAGTACT215000.021.19043212-13
ACCTGGG43300.021.162363
ATGGGGG52550.021.0141815
Produced by FastQC (version 0.11.5)