##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211556_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4973157 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.157231110942206 33.0 31.0 34.0 30.0 34.0 2 32.36001839475408 34.0 31.0 34.0 31.0 34.0 3 32.36726992532108 34.0 31.0 34.0 30.0 34.0 4 35.99639142701508 37.0 35.0 37.0 35.0 37.0 5 35.93765589946185 37.0 35.0 37.0 35.0 37.0 6 35.96532846238315 37.0 35.0 37.0 35.0 37.0 7 35.89798994883934 37.0 35.0 37.0 35.0 37.0 8 35.89533449275782 37.0 35.0 37.0 35.0 37.0 9 37.551330874935175 39.0 37.0 39.0 35.0 39.0 10-11 37.5208047926096 39.0 37.0 39.0 35.0 39.0 12-13 37.44513424772232 39.0 37.0 39.0 34.0 39.0 14-15 38.6679583009344 40.0 38.0 41.0 34.0 41.0 16-17 38.61605434938008 40.0 38.0 41.0 34.0 41.0 18-19 38.651112261285945 40.0 38.0 41.0 34.0 41.0 20-21 38.614633823142924 40.0 38.0 41.0 34.0 41.0 22-23 38.51197609486288 40.0 38.0 41.0 34.0 41.0 24-25 38.41239116319875 40.0 38.0 41.0 34.0 41.0 26-27 38.241686015543046 40.0 38.0 41.0 34.0 41.0 28-29 38.27354967880564 40.0 38.0 41.0 34.0 41.0 30-31 38.17618355905515 40.0 37.5 41.0 34.0 41.0 32-33 37.966254936250756 40.0 37.0 41.0 33.0 41.0 34-35 37.84438757915747 40.0 37.0 41.0 33.0 41.0 36-37 37.77917900038145 40.0 37.0 41.0 33.0 41.0 38-39 37.68034600556548 40.0 37.0 41.0 33.0 41.0 40-41 37.5236722870402 40.0 37.0 41.0 32.0 41.0 42-43 37.37012837117348 40.0 36.5 41.0 31.5 41.0 44-45 37.168694453040594 40.0 36.0 41.0 31.0 41.0 46-47 36.87798575432065 40.0 36.0 41.0 30.0 41.0 48-49 36.62112105047156 39.0 36.0 41.0 30.0 41.0 50-51 36.42274042424158 39.0 35.5 41.0 29.0 41.0 52-53 36.25164990769445 39.0 35.0 41.0 28.5 41.0 54-55 36.08141518556523 39.0 35.0 40.0 28.0 41.0 56-57 35.91013665967111 39.0 35.0 40.0 28.0 41.0 58-59 35.692791017858475 38.5 35.0 40.0 27.0 41.0 60-61 35.417902853258 38.0 34.0 40.0 26.0 41.0 62-63 35.124051080631475 38.0 34.0 40.0 26.0 41.0 64-65 34.84528871700612 38.0 34.0 40.0 26.0 41.0 66-67 34.53477599842515 37.0 33.5 40.0 25.5 41.0 68-69 34.160931677805465 36.5 33.0 39.0 25.0 41.0 70-71 33.79038566447832 36.0 32.5 39.0 24.0 40.0 72-73 33.393389953303306 36.0 32.0 39.0 23.0 40.0 74-75 32.98396400917969 35.0 32.0 38.0 22.5 40.0 76-77 31.378705417906573 34.0 29.5 36.0 21.0 39.0 78-79 32.20580528625982 35.0 31.0 37.0 22.0 39.0 80-81 32.1500055196327 35.0 31.0 36.0 22.0 39.0 82-83 31.84021538029063 35.0 31.0 36.0 21.0 37.5 84-85 31.512139773588487 34.0 31.0 35.5 20.0 37.0 86-87 31.20338046838256 34.0 31.0 35.0 20.0 37.0 88-89 30.90023449893096 34.0 30.0 35.0 19.5 36.0 90-91 30.59781804596155 34.0 30.0 35.0 19.0 36.0 92-93 30.373596691196354 34.0 29.5 35.0 18.0 36.0 94-95 30.145514609733816 34.0 29.0 35.0 18.0 35.0 96-97 29.94076850982183 34.0 29.0 35.0 17.5 35.0 98-99 29.77993174154767 34.0 29.0 35.0 17.0 35.0 100 27.969325520991998 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33566936771480727 1101 2 0.2644959932704438 1101 3 0.34898038599129677 1101 4 0.1813652051277046 1101 5 0.2376746153020406 1101 6 0.21903025932729037 1101 7 0.21814602306864828 1101 8 0.22924382870123594 1101 9 0.288446635003055 1101 10-11 0.28108836356187794 1101 12-13 0.2644129555264385 1101 14-15 0.35065299516509185 1101 16-17 0.36097610957479276 1101 18-19 0.37614852155944334 1101 20-21 0.36109000775245903 1101 22-23 0.3543094468189878 1101 24-25 0.37399521891834553 1101 26-27 0.28450053961263677 1101 28-29 0.38831406273479985 1101 30-31 0.45459141897175215 1101 32-33 0.5591048492304012 1101 34-35 0.4790536026600307 1101 36-37 0.4853971136097357 1101 38-39 0.4618885001108737 1101 40-41 0.4562230500564297 1101 42-43 0.4513346638902789 1101 44-45 0.5234977116377948 1101 46-47 0.6209534812993738 1101 48-49 0.5646973423939983 1101 50-51 0.6359468604109324 1101 52-53 0.545791941464806 1101 54-55 0.5060934418234737 1101 56-57 0.5012161854008212 1101 58-59 0.5504853884573748 1101 60-61 0.44815727465483945 1101 62-63 0.471450702620551 1101 64-65 0.5294702517005945 1101 66-67 0.5390379856978171 1101 68-69 0.5658874583602014 1101 70-71 0.586052500707197 1101 72-73 0.37632230921786913 1101 74-75 0.4788631613370029 1101 76-77 0.42826551282343317 1101 78-79 0.4628082032485281 1101 80-81 0.5331815089771723 1101 82-83 0.3474541307759047 1101 84-85 0.3483229787840081 1101 86-87 0.30899710885307385 1101 88-89 0.3458592137194394 1101 90-91 0.25758154673905764 1101 92-93 0.23512691582536505 1101 94-95 0.2387741397828833 1101 96-97 0.22187525525788843 1101 98-99 0.05607735683578241 1101 100 0.036283669279040254 1102 1 0.3139072161084897 1102 2 0.2933103632444869 1102 3 0.36280188723629436 1102 4 0.18813349571310312 1102 5 0.24608471895023598 1102 6 0.22136680900936057 1102 7 0.22918659325279123 1102 8 0.2315233216003918 1102 9 0.301102755591657 1102 10-11 0.24904140434110644 1102 12-13 0.27464540320619335 1102 14-15 0.3454489700323222 1102 16-17 0.3704136487703238 1102 18-19 0.31070712036392933 1102 20-21 0.36206238763882936 1102 22-23 0.30699450594635636 1102 24-25 0.35340370711536906 1102 26-27 0.32547723266104356 1102 28-29 0.41166386480563943 1102 30-31 0.4445537581873893 1102 32-33 0.5466212881991552 1102 34-35 0.507463927920071 1102 36-37 0.5191588524226347 1102 38-39 0.4379589845874605 1102 40-41 0.40325614695968426 1102 42-43 0.4867729625198578 1102 44-45 0.4845586238054054 1102 46-47 0.502680558695495 1102 48-49 0.5722960489746143 1102 50-51 0.5571353019305363 1102 52-53 0.5502976742657424 1102 54-55 0.5256320657924789 1102 56-57 0.5395800416322842 1102 58-59 0.48153845159624353 1102 60-61 0.4781238939561021 1102 62-63 0.4781642517707567 1102 64-65 0.46317721600190254 1102 66-67 0.4885248054155795 1102 68-69 0.49827192912464113 1102 70-71 0.4332667735689384 1102 72-73 0.4901586452264226 1102 74-75 0.4512260464454769 1102 76-77 0.3933674675061205 1102 78-79 0.47265549283634556 1102 80-81 0.5155962115674484 1102 82-83 0.5011859089894095 1102 84-85 0.5086842921021493 1102 86-87 0.47881299571062996 1102 88-89 0.36922153520612966 1102 90-91 0.43319438620210704 1102 92-93 0.30662584547319227 1102 94-95 0.40004320170983476 1102 96-97 0.4126224973479964 1102 98-99 0.37532277509342293 1102 100 0.39299510153990624 1103 1 0.2391925568734692 1103 2 0.16614349565199404 1103 3 0.26832069337157805 1103 4 0.1324492519509377 1103 5 0.18031654299139888 1103 6 0.19265131214849873 1103 7 0.17222346966514124 1103 8 0.16628009697576118 1103 9 0.1512999629836429 1103 10-11 0.1493637337237672 1103 12-13 0.20010857637294066 1103 14-15 0.34006595191767985 1103 16-17 0.2934502185750887 1103 18-19 0.3372190545750726 1103 20-21 0.3371529347311508 1103 22-23 0.2554481925438239 1103 24-25 0.269064700889281 1103 26-27 0.20744831499887795 1103 28-29 0.3081501744506099 1103 30-31 0.4291000180966833 1103 32-33 0.5457768778512175 1103 34-35 0.5217465901089611 1103 36-37 0.5984084336097197 1103 38-39 0.4911822784140796 1103 40-41 0.557593307016667 1103 42-43 0.5279720526129168 1103 44-45 0.5486831221432098 1103 46-47 0.6114538413015822 1103 48-49 0.6357408569991136 1103 50-51 0.5845338213420561 1103 52-53 0.5658471150656865 1103 54-55 0.6609313408938604 1103 56-57 0.6652868047433387 1103 58-59 0.6782386689614555 1103 60-61 0.6853004686144004 1103 62-63 0.6674252231035993 1103 64-65 0.644065255957436 1103 66-67 0.7045100856418713 1103 68-69 0.7752261937100258 1103 70-71 0.713199306852168 1103 72-73 0.6514728240660617 1103 74-75 0.6160578950152527 1103 76-77 0.6368682999131074 1103 78-79 0.6151873474078045 1103 80-81 0.615383086675088 1103 82-83 0.54388140720717 1103 84-85 0.4098268294039009 1103 86-87 0.5122553704451214 1103 88-89 0.6200086686469746 1103 90-91 0.608667214246136 1103 92-93 0.44592158741136956 1103 94-95 0.5255622165992229 1103 96-97 0.4595824504826993 1103 98-99 0.5291517493801372 1103 100 0.6236233078724602 1104 1 0.30260530403931085 1104 2 0.25132095878705485 1104 3 0.31202751951818897 1104 4 0.11743869605411561 1104 5 0.19164199983360675 1104 6 0.2334070598923219 1104 7 0.2544382881336418 1104 8 0.25695059395861364 1104 9 0.2941481621600843 1104 10-11 0.3304997123019717 1104 12-13 0.3386581097380841 1104 14-15 0.42629453289514885 1104 16-17 0.3771091893053793 1104 18-19 0.3793246760818718 1104 20-21 0.3018068361428732 1104 22-23 0.353559327527158 1104 24-25 0.368395790905403 1104 26-27 0.4081782840961097 1104 28-29 0.4956287188632089 1104 30-31 0.4040334196171713 1104 32-33 0.3524752239763629 1104 34-35 0.46322119459486544 1104 36-37 0.3632704100891857 1104 38-39 0.47032917904206073 1104 40-41 0.536487229247669 1104 42-43 0.5842725058154343 1104 44-45 0.4454317411682993 1104 46-47 0.42200321529436735 1104 48-49 0.4756878404738174 1104 50-51 0.5501776638028204 1104 52-53 0.551829156423814 1104 54-55 0.5484551080528632 1104 56-57 0.5193876168362763 1104 58-59 0.5704737721012592 1104 60-61 0.5205598538473097 1104 62-63 0.5218114932760471 1104 64-65 0.6662990444434271 1104 66-67 0.6610651983843638 1104 68-69 0.6502098782173178 1104 70-71 0.5997715638908616 1104 72-73 0.5980735865652775 1104 74-75 0.7269466245317844 1104 76-77 0.5663965342052641 1104 78-79 0.6310966591579401 1104 80-81 0.6476350415922099 1104 82-83 0.5347247593121516 1104 84-85 0.5353304578148119 1104 86-87 0.6217419426517274 1104 88-89 0.6547115283048193 1104 90-91 0.5174116963145572 1104 92-93 0.566363798625467 1104 94-95 0.5192095639068874 1104 96-97 0.4956670919974506 1104 98-99 0.5367446989354221 1104 100 0.709834808437158 1105 1 0.25125249311159337 1105 2 0.1602060345964631 1105 3 0.22082504208048448 1105 4 0.12060348338054894 1105 5 0.11588503405641859 1105 6 0.135549883572061 1105 7 0.12096406649007463 1105 8 0.1385500829853683 1105 9 0.16002451576468957 1105 10-11 0.16590720985958995 1105 12-13 0.21066346520993307 1105 14-15 0.2130143252659309 1105 16-17 0.2894052723529512 1105 18-19 0.2248041433696102 1105 20-21 0.2232061058918191 1105 22-23 0.23909646463894774 1105 24-25 0.25851732380454706 1105 26-27 0.2536563956985276 1105 28-29 0.3265518496176867 1105 30-31 0.3764708783352688 1105 32-33 0.434712245477364 1105 34-35 0.4789373456635104 1105 36-37 0.4095362606676076 1105 38-39 0.4510482105130009 1105 40-41 0.5137602802312173 1105 42-43 0.4696439747485357 1105 44-45 0.5107370423612494 1105 46-47 0.5390546134435326 1105 48-49 0.6414743756355747 1105 50-51 0.6367052405873821 1105 52-53 0.6010340984631242 1105 54-55 0.5696025635430573 1105 56-57 0.6722040530965003 1105 58-59 0.6633991196586493 1105 60-61 0.6779031464912038 1105 62-63 0.6081534441346861 1105 64-65 0.6235417589647554 1105 66-67 0.7170916915644909 1105 68-69 0.7590635501768261 1105 70-71 0.7681350509263112 1105 72-73 0.7781291902562373 1105 74-75 0.7135393600147566 1105 76-77 0.6738852884522935 1105 78-79 0.8008708311559687 1105 80-81 0.7734419360586244 1105 82-83 0.7529922758390661 1105 84-85 0.7619994995048778 1105 86-87 0.7542307011888987 1105 88-89 0.6653453695396507 1105 90-91 0.7831570289388203 1105 92-93 0.7256841094858295 1105 94-95 0.797245402392253 1105 96-97 0.8730432093867577 1105 98-99 0.8654769220825997 1105 100 0.9153427691415033 1106 1 0.259821758837127 1106 2 0.16055174255482996 1106 3 0.22335910862560127 1106 4 0.16806852819686213 1106 5 0.18629586337802095 1106 6 0.1863405721278042 1106 7 0.16615560874504354 1106 8 0.13943186732753077 1106 9 0.1090050544422354 1106 10-11 0.18144395306674 1106 12-13 0.1810297110522754 1106 14-15 0.2788541424461144 1106 16-17 0.2931179216338933 1106 18-19 0.265682757545342 1106 20-21 0.28952990388071953 1106 22-23 0.32084070784540586 1106 24-25 0.30229176308738204 1106 26-27 0.3291316554496433 1106 28-29 0.3402089672544193 1106 30-31 0.40771592710292737 1106 32-33 0.40404882443103673 1106 34-35 0.3932179259210784 1106 36-37 0.3722323156910079 1106 38-39 0.35274534140904734 1106 40-41 0.4143037481590781 1106 42-43 0.43287803498362365 1106 44-45 0.4993574743801261 1106 46-47 0.5354141918789068 1106 48-49 0.5754569685148425 1106 50-51 0.4941417986821577 1106 52-53 0.5544893284386561 1106 54-55 0.4985893371568011 1106 56-57 0.5584018807127435 1106 58-59 0.596721485079506 1106 60-61 0.5796418849606013 1106 62-63 0.5884715980975912 1106 64-65 0.6386832130034037 1106 66-67 0.6012434460952392 1106 68-69 0.5816427437173459 1106 70-71 0.5967725206953745 1106 72-73 0.6029178906037345 1106 74-75 0.6574854167603164 1106 76-77 0.6006263380708425 1106 78-79 0.6784307302176487 1106 80-81 0.5669080556454063 1106 82-83 0.5642918560159025 1106 84-85 0.6060418633165447 1106 86-87 0.6308713415377163 1106 88-89 0.6951023448013345 1106 90-91 0.5845249709097544 1106 92-93 0.6929951608155172 1106 94-95 0.7739000232990385 1106 96-97 0.8039705733100178 1106 98-99 0.8459530849385004 1106 100 0.9028214383714896 1107 1 0.20619020803245292 1107 2 0.2151626468818506 1107 3 0.23093228436840008 1107 4 0.10484514128204836 1107 5 0.14777015035774355 1107 6 0.14101603408136754 1107 7 0.18054113726488197 1107 8 0.17099301429162495 1107 9 0.18879127702958698 1107 10-11 0.16759762191797023 1107 12-13 0.22961116814336435 1107 14-15 0.296002079425854 1107 16-17 0.21916104685176663 1107 18-19 0.1829829217145047 1107 20-21 0.17083663523391124 1107 22-23 0.25838927212329565 1107 24-25 0.2698749945742591 1107 26-27 0.320714108099736 1107 28-29 0.29980948685915365 1107 30-31 0.2246954383678954 1107 32-33 0.23351272835771653 1107 34-35 0.2793893442942803 1107 36-37 0.3674367453930216 1107 38-39 0.38636213270722664 1107 40-41 0.35819046364342455 1107 42-43 0.31650571455634946 1107 44-45 0.2971626333148478 1107 46-47 0.28985807542979813 1107 48-49 0.36567803213407757 1107 50-51 0.26829010331069725 1107 52-53 0.3726325566482629 1107 54-55 0.4327891571299176 1107 56-57 0.4851255839474149 1107 58-59 0.5173004943779702 1107 60-61 0.40369510584505264 1107 62-63 0.45680340802302055 1107 64-65 0.5257805867974881 1107 66-67 0.5338089120633072 1107 68-69 0.4909109536001566 1107 70-71 0.602756185710156 1107 72-73 0.5833713860215397 1107 74-75 0.5465980602901084 1107 76-77 0.5697405006036007 1107 78-79 0.5635952527068255 1107 80-81 0.5773487146534464 1107 82-83 0.577959213977298 1107 84-85 0.4528549860277771 1107 86-87 0.4561044152958864 1107 88-89 0.5317137697902652 1107 90-91 0.44610212095970425 1107 92-93 0.506114672686973 1107 94-95 0.5739657250464205 1107 96-97 0.6121992989917118 1107 98-99 0.6742243385304825 1107 100 0.7206228039718603 1108 1 0.23652750172978187 1108 2 0.18835942215487478 1108 3 0.23340616652809132 1108 4 0.15251729568823436 1108 5 0.13962823954889103 1108 6 0.13960721751607963 1108 7 0.17633481255194283 1108 8 0.1635144147885086 1108 9 0.1582658791148006 1108 10-11 0.19609657973861516 1108 12-13 0.20078323122481834 1108 14-15 0.21061282718991947 1108 16-17 0.26320360637961926 1108 18-19 0.19192358089849648 1108 20-21 0.21858498675555893 1108 22-23 0.23398810301763717 1108 24-25 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-0.5841899321831399 2316 78-79 -0.4623353515741222 2316 80-81 -0.40342532205031745 2316 82-83 -0.17415144006153227 2316 84-85 -0.3423714264934574 2316 86-87 -0.4725883438209628 2316 88-89 -0.421189038009814 2316 90-91 -0.3098048246292322 2316 92-93 -0.18358866865375134 2316 94-95 -0.18016284529502613 2316 96-97 -0.20424546898080465 2316 98-99 -0.19361650207740766 2316 100 -0.32488213752065676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 10.0 11 20.0 12 49.0 13 158.0 14 447.0 15 1352.0 16 3368.0 17 7566.0 18 13781.0 19 21300.0 20 29781.0 21 40919.0 22 51705.0 23 53332.0 24 50730.0 25 51644.0 26 57231.0 27 68607.0 28 82001.0 29 99037.0 30 119905.0 31 145605.0 32 178618.0 33 223959.0 34 293620.0 35 400893.0 36 589008.0 37 975387.0 38 1180336.0 39 232716.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.1245176701293 21.166632515563553 13.918106123932914 22.790743690374235 2 14.273514283173098 22.702801901337626 41.10163608243315 21.922047733056125 3 16.76759601022779 29.05514369662803 31.493836057715125 22.683424235429055 4 10.649548543965562 18.629631915990146 40.72293691841216 29.997882621632126 5 10.889176436759074 40.208401827034926 36.50562980524162 12.396791930964381 6 27.624644564248484 40.41624967500288 19.260219085761236 12.698886674987397 7 24.918517553336844 34.186352853931616 24.26567269040571 16.629456902325828 8 24.8165581931442 36.750937995854905 21.60866163439607 16.823842176604828 9 26.515375749322967 14.819422515601763 21.751398910470535 36.91380282460474 10-11 23.12452633206633 27.811368110839858 30.133967216397956 18.93013834069586 12-13 24.115989099077307 26.01066083375208 30.02661086307953 19.846739204091083 14-15 21.49229352702921 26.994241283755972 28.355559657577672 23.157905531637148 16-17 19.977673970801824 29.856323566759613 29.13200950952511 21.03399295291345 18-19 19.80243502138908 28.71382184706595 31.694911300762623 19.788831830782343 20-21 20.935769435683522 27.886334572592368 31.717323059719405 19.460572932004705 22-23 20.439732749237557 28.13137610576139 31.259942125293854 20.1689490197072 24-25 20.09120566497411 28.16362486919775 31.741151896300863 20.004017569527278 26-27 20.12403202717955 28.272296578165435 31.65606674721922 19.947604647435803 28-29 19.964734458292988 28.17971752779665 31.888119073584797 19.96742894032557 30-31 20.365611428217527 28.12346800603407 31.738631926120842 19.772288639627565 32-33 20.032006923807252 28.378872534642912 31.616775955454536 19.972344586095296 34-35 20.321579746428174 28.454277642043458 31.646668476457634 19.577474135070734 36-37 20.341975933112927 28.247882079954778 31.478252441611605 19.93188954532069 38-39 20.14627590051051 28.64374667710154 31.472041001651295 19.737936420736656 40-41 20.345054125664202 28.432211808387585 31.519079920014025 19.70365414593418 42-43 20.18393104471084 28.37833911060949 31.21325815752232 20.224471687157354 44-45 20.210580377489844 28.70221665513243 31.063908597751944 20.023294369625784 46-47 20.22793254567308 28.71154545730593 30.737139957935057 20.32338203908593 48-49 20.189502856147136 28.830922786243395 30.744130622918696 20.235443734690772 50-51 20.378281150283744 28.957216130018608 30.34735531688145 20.317147402816193 52-53 20.400530751204794 29.09344681925814 30.15686961757017 20.349152811966896 54-55 20.329891301724984 29.17359067868455 30.14125989939337 20.355258120197096 56-57 20.448891796116143 29.13569889848216 29.95117480289247 20.46423450250923 58-59 20.373306586323757 29.04604991296028 30.19212482080797 20.388518679907992 60-61 20.579034871497353 29.381736390724868 29.93462882515385 20.104599912623932 62-63 20.274999059813368 29.216505775762574 30.18822636959048 20.32026879483358 64-65 20.150190364663427 28.981498024665616 30.16272717501695 20.70558443565401 66-67 20.204286162313814 29.069667708948476 29.93120175860295 20.79484437013476 68-69 20.242635953560303 29.16463045528681 29.955726228614754 20.637007362538135 70-71 20.244319973359417 28.98253400857132 29.944705907903373 20.828440110165893 72-73 20.245125813118587 29.1277215184146 29.778055513520936 20.849097154945877 74-75 20.184651060330467 29.279530681822635 29.575277626600354 20.96054063124654 76-77 19.978638751793675 29.07143621265451 29.915168316099017 21.03475671945279 78-79 20.037510311162844 29.006482508929636 29.772233400887956 21.183773779019564 80-81 19.984871934784625 29.321832142825183 29.50296473278594 21.190331189604247 82-83 20.176943595131366 29.163233493265416 29.565188798224735 21.094634113378486 84-85 20.014136484347446 29.188863652110154 29.473876012232314 21.32312385131009 86-87 19.793810244563854 29.236499349805467 29.65206874091344 21.31762166471724 88-89 20.04450900563201 28.964572299730847 29.603718000352973 21.38720069428417 90-91 20.1736455200908 29.00973741580855 29.511652898828665 21.304964165271983 92-93 20.05205672229522 29.020511302055716 29.42591768604963 21.501514289599438 94-95 19.773658470250947 28.86431336544953 29.52129863797184 21.84072952632769 96-97 19.871854437034838 28.82146830884784 29.512840501986755 21.793836752130563 98-99 19.732826054864752 28.711429694253237 29.464654554142932 22.09108969673908 100 19.005282228350588 29.086546970481947 29.62241238387303 22.285758417294428 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2542.0 1 1777.5 2 1572.0 3 2208.5 4 1837.0 5 1671.0 6 2685.5 7 5120.0 8 7911.0 9 9190.0 10 8082.5 11 6759.5 12 7110.0 13 7718.5 14 8213.5 15 8847.0 16 9570.5 17 10351.0 18 11415.5 19 12569.5 20 14368.5 21 16770.5 22 19778.5 23 24262.5 24 29455.0 25 35693.0 26 43937.5 27 53176.5 28 62252.5 29 73189.5 30 86829.5 31 99647.5 32 115032.5 33 134011.5 34 153634.0 35 168915.0 36 183202.0 37 195700.5 38 200743.5 39 204938.5 40 200394.5 41 191628.0 42 186192.5 43 181771.5 44 180881.5 45 181675.0 46 178412.0 47 172709.5 48 169596.0 49 165026.0 50 160247.0 51 147689.5 52 128703.0 53 113884.5 54 101257.5 55 88492.0 56 76177.5 57 64978.0 58 54347.0 59 44618.5 60 35115.5 61 27182.5 62 21183.5 63 16024.0 64 11657.5 65 8294.0 66 5934.0 67 4454.5 68 3378.0 69 2614.5 70 1993.0 71 1420.5 72 1030.0 73 753.5 74 587.5 75 430.0 76 289.0 77 196.0 78 149.5 79 110.0 80 64.5 81 43.0 82 37.0 83 26.0 84 16.5 85 12.0 86 9.0 87 8.0 88 5.0 89 3.5 90 4.0 91 3.5 92 3.0 93 2.5 94 2.5 95 2.5 96 2.0 97 1.5 98 2.5 99 3.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032172722477894827 2 0.07244894942990941 3 8.244260134960548E-4 4 5.22806740265791E-4 5 0.0014477725115052671 6 7.238862557526336E-4 7 0.0 8 0.0010858293836289504 9 2.0107951548684264E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.641021588500021E-4 18-19 0.0011863691413723718 20-21 0.0031871103204664563 22-23 0.0 24-25 2.0107951548684264E-5 26-27 1.206477092921056E-4 28-29 6.132925222348701E-4 30-31 0.0012668009475671088 32-33 0.0034284057390506673 34-35 0.004162345970577643 36-37 0.0011763151655980295 38-39 0.0017896076878328995 40-41 0.0057307661913750165 42-43 0.007429888097238837 44-45 0.005660388360954621 46-47 0.007439942073013179 48-49 0.009279819639717789 50-51 0.009118956027328314 52-53 0.004132184043254617 54-55 0.0025134939435855334 56-57 0.002211874670355269 58-59 0.0028151132168157973 60-61 0.008033126643699364 62-63 0.014879884146026357 64-65 0.004051752237059879 66-67 0.011531910213170427 68-69 0.013814162713946091 70-71 0.00589162980376449 72-73 0.008133666401442786 74-75 0.0031267864658204034 76-77 0.0026743575559750074 78-79 0.007862209055535549 80-81 0.005640280409405937 82-83 0.016850463397797416 84-85 0.01842893759436913 86-87 0.02379776065786783 88-89 0.02271193127423888 90-91 0.026220768819484284 92-93 0.037179602413517206 94-95 0.04104032911086459 96-97 0.024793104259527698 98-99 0.02016827540333032 100 0.020369354918817164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4973157.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.28441724257681 #Duplication Level Percentage of deduplicated Percentage of total 1 74.24221600356812 44.75648726572613 2 16.406103383018706 19.780647633335015 3 5.094973535129179 9.214425312948418 4 1.9325336942913334 4.6600667024798845 5 0.8894054042758356 2.6808643244583585 6 0.4556608095221055 1.6481547817392552 7 0.2403399415273394 1.0142127320563448 8 0.15990026356617396 0.7711595364816985 9 0.10403794137071648 0.5644679999985914 >10 0.39928431885547244 4.238562381324475 >50 0.0347913243732963 1.475716779067667 >100 0.035557832847959304 4.350849312602164 >500 0.0035224052226198333 1.4317173383899449 >1k 0.0015410522389600523 1.5830404722699163 >5k 0.0 0.0 >10k+ 1.320901919108614E-4 1.8296274271221533 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27303 0.5490074011337266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24167 0.48594886507705265 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17460 0.3510848340400273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0107951548684268E-5 0.0 4 0.0 0.0 0.0 2.0107951548684268E-5 0.0 5 0.0 4.0215903097368535E-5 0.0 2.0107951548684268E-5 0.0 6 0.0 4.0215903097368535E-5 0.0 2.0107951548684268E-5 0.0 7 0.0 4.0215903097368535E-5 0.0 2.0107951548684268E-5 0.0 8 0.0 4.0215903097368535E-5 0.0 2.0107951548684268E-5 0.0 9 0.0 4.0215903097368535E-5 0.0 2.0107951548684268E-5 0.0 10-11 0.0 4.0215903097368535E-5 0.0 5.026987887171067E-5 0.0 12-13 0.0 7.037783042039493E-5 0.0 8.043180619473707E-5 0.0 14-15 0.0 8.043180619473707E-5 0.0 1.206477092921056E-4 0.0 16-17 0.0 8.043180619473707E-5 0.0 1.4075566084078986E-4 0.0 18-19 0.0 8.043180619473707E-5 0.0 1.7091758816381627E-4 0.0 20-21 0.0 8.043180619473707E-5 0.0 1.9102553971250054E-4 0.0 22-23 0.0 8.043180619473707E-5 0.0 2.412954185842112E-4 0.0 24-25 0.0 8.043180619473707E-5 0.0 3.217272247789482E-4 0.0 26-27 0.0 8.043180619473707E-5 0.0 5.32860716040133E-4 0.0 28-29 0.0 8.043180619473707E-5 0.0 0.0019303633486736894 0.0 30-31 0.0 8.043180619473707E-5 0.0 0.0075505358065309424 0.0 32-33 0.0 8.043180619473707E-5 0.0 0.018790880722245447 0.0 34-35 0.0 8.043180619473707E-5 0.0 0.035229131113294836 0.0 36-37 0.0 9.048578196907921E-5 0.0 0.062173786188531754 0.0 38-39 0.0 1.1059373351776346E-4 0.0 0.10811040150150096 0.0 40-41 0.0 1.206477092921056E-4 0.0 0.15837022639743728 0.0 42-43 0.0 1.206477092921056E-4 0.0 0.20698119926638148 0.0 44-45 0.0 1.3070168506644774E-4 0.0 0.2668526250025889 0.0 46-47 0.0 1.4075566084078986E-4 0.0 0.3398545431000871 0.0 48-49 0.0 1.4075566084078986E-4 0.0 0.42165369000013475 0.0 50-51 0.0 1.4075566084078986E-4 0.0 0.5151858266288396 0.0 52-53 0.0 1.4075566084078986E-4 0.0 0.6055610148644011 0.0 54-55 0.0 2.412954185842112E-4 0.0 0.7070458463306105 0.0 56-57 0.0 2.815113216815797E-4 0.0 0.8281862004356588 0.0 58-59 0.0 3.4183517632763253E-4 0.0 0.9653726998765573 0.0 60-61 0.0 3.4183517632763253E-4 0.0 1.1172279499722209 0.0 62-63 0.0 3.4183517632763253E-4 0.0 1.2760405513037292 0.0 64-65 0.0 3.719971036506589E-4 0.0 1.4320782553215192 0.0 66-67 0.0 3.820510794250011E-4 0.0 1.6088170954586793 0.0 68-69 0.0 4.0215903097368534E-4 0.0 1.816331155441101 0.0 70-71 0.0 4.825908371684224E-4 0.0 2.0563698270535196 0.0 72-73 0.0 5.429146918144752E-4 0.0 2.303627253271916 0.0 74-75 0.0 5.429146918144752E-4 0.0 2.5400967634844425 0.0 76-77 0.0 5.429146918144752E-4 0.0 2.793034686015342 0.0 78-79 0.0 5.429146918144752E-4 0.0 3.0947243370760265 0.0 80-81 0.0 5.630226433631595E-4 0.0 3.432296627675338 0.0 82-83 0.0 5.630226433631595E-4 0.0 3.7892027136887094 0.0 84-85 0.0 5.630226433631595E-4 0.0 4.129811304971872 0.0 86-87 0.0 5.630226433631595E-4 0.0 4.4727222567073595 0.0 88 0.0 5.630226433631595E-4 0.0 4.753097479126438 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15015 0.0 41.062386 1 GTATCAA 24320 0.0 32.583908 1 GTACATG 28210 0.0 28.140781 1 TACCTGG 3550 0.0 27.422611 2 TACATGG 28505 0.0 27.222609 2 ACATGGG 27445 0.0 27.121223 3 ATCAACG 29495 0.0 26.30365 3 TCAACGC 29750 0.0 26.07819 4 CAACGCA 29985 0.0 25.873808 5 TAGATCG 295 0.0 25.486801 5 AACGCAG 30825 0.0 25.244955 6 TATCAAC 30920 0.0 25.20286 2 CATGGGG 9430 0.0 24.96568 4 GTACCTG 4300 0.0 23.29582 1 CATGGGA 15605 0.0 22.885857 4 ACGCAGA 35130 0.0 22.03093 7 CGCAGAG 35220 0.0 21.881237 8 GAGTACT 21500 0.0 21.190432 12-13 ACCTGGG 4330 0.0 21.16236 3 ATGGGGG 5255 0.0 21.014181 5 >>END_MODULE