FastQCFastQC Report
Mon 6 Jun 2016
ERR1211555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517393
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81960.5401369322252046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69190.4559794331461922No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53790.3544895752122225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.32641510801750107No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26550.17497115117836973No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16100.10610303329460463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA3200.066.076184
TACCTGG25200.064.930312
GTACCTG28700.057.9018061
TATAACG3500.057.76562
ACCTGGG28650.055.7617843
ATAACGC3800.051.933563
CCTGGGG25050.040.8913234
GTATAAC5700.039.642921
GGTATCA50800.036.6970141
TATCACG1950.036.1680832
GTACCCG3650.033.5335661
CTGGGGG17550.032.393815
TGGGGGG37750.030.984916
ATGGGGG29000.030.4588585
ATATCTA5400.029.6404251
CATGGGG41300.029.466744
GTATCAA74700.029.4303571
GTGGTTA12800.029.422481
GACCGTA804.7955193E-429.3604157
CCCTAGA6950.029.1261371
Produced by FastQC (version 0.11.5)