##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211555_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1517393 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.143514567419253 31.0 31.0 34.0 28.0 34.0 2 31.13270985169959 31.0 31.0 34.0 28.0 34.0 3 31.22037072795248 31.0 31.0 34.0 27.0 34.0 4 34.76264685549492 37.0 35.0 37.0 32.0 37.0 5 34.86487943466195 37.0 35.0 37.0 32.0 37.0 6 34.9819315101625 37.0 35.0 37.0 32.0 37.0 7 34.93485076048196 37.0 35.0 37.0 32.0 37.0 8 34.898445557610984 37.0 35.0 37.0 32.0 37.0 9 36.44225853157356 39.0 37.0 39.0 32.0 39.0 10-11 36.40188995204275 39.0 37.0 39.0 32.0 39.0 12-13 36.3239552970127 39.0 37.0 39.0 32.0 39.0 14-15 37.462376589321295 40.0 37.0 41.0 32.0 41.0 16-17 37.43038883137065 40.0 37.0 41.0 31.5 41.0 18-19 37.44122715736793 40.0 37.0 41.0 31.0 41.0 20-21 37.392430306453235 40.0 37.0 41.0 31.0 41.0 22-23 37.228168971387106 40.0 37.0 41.0 31.0 41.0 24-25 37.13326409176792 40.0 36.0 41.0 31.0 41.0 26-27 36.9714895877337 40.0 36.0 41.0 30.0 41.0 28-29 36.77176117195743 39.5 36.0 41.0 30.0 41.0 30-31 36.5444400362991 39.0 35.5 41.0 30.0 41.0 32-33 36.277768844327085 39.0 35.0 41.0 29.0 41.0 34-35 36.058256166991676 39.0 35.0 40.0 27.5 41.0 36-37 35.860823464982374 39.0 35.0 40.0 27.0 41.0 38-39 35.70827102800659 38.5 35.0 40.0 26.5 41.0 40-41 35.65102448739384 38.5 35.0 40.0 26.0 41.0 42-43 35.57503560382841 38.5 35.0 40.0 26.0 41.0 44-45 35.37414170224853 38.0 34.5 40.0 25.0 41.0 46-47 35.258530914535655 38.0 34.0 40.0 24.0 41.0 48-49 35.19234107446126 39.0 34.0 40.0 24.0 41.0 50-51 34.18925551917005 38.0 33.0 39.5 22.5 40.5 52-53 34.39774336641859 38.0 33.5 39.5 23.0 40.5 54-55 34.96012140559499 38.0 34.0 40.0 23.0 41.0 56-57 34.90049677308383 38.0 34.0 40.0 23.0 41.0 58-59 34.73514442204492 38.0 34.0 40.0 23.0 41.0 60-61 34.50866189576465 38.0 33.0 40.0 23.0 41.0 62-63 34.18719606588405 37.0 33.0 40.0 22.0 41.0 64-65 33.8962098151237 36.5 33.0 40.0 22.0 41.0 66-67 33.58991078777878 36.0 32.5 39.0 21.5 41.0 68-69 33.3011154658022 35.5 32.0 39.0 21.0 41.0 70-71 32.938054610769925 35.0 32.0 38.5 20.0 40.5 72-73 32.5827745350084 35.0 32.0 37.5 20.0 40.0 74-75 32.23669313091598 35.0 31.0 37.0 20.0 39.0 76-77 31.909074972666936 35.0 31.0 36.5 19.0 39.0 78-79 31.607377258231715 35.0 31.0 36.0 19.0 39.0 80-81 31.310315455521412 35.0 31.0 36.0 18.0 37.5 82-83 31.02569670480884 35.0 30.0 35.0 18.0 37.0 84-85 30.775035867438426 34.0 30.0 35.0 17.5 36.5 86-87 30.49734083391712 34.0 30.0 35.0 17.0 36.0 88-89 30.299773031772258 34.0 29.5 35.0 15.5 36.0 90-91 30.078150815246943 34.0 29.0 35.0 15.0 35.5 92-93 29.913287131283724 34.0 29.0 35.0 14.0 35.0 94-95 29.75579035885891 34.0 29.0 35.0 12.0 35.0 96-97 29.64906948957851 34.0 29.0 35.0 11.0 35.0 98-99 29.468473889097943 34.0 29.0 35.0 10.0 35.0 100 28.10699469418931 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3475534898459962 1101 2 0.3563814188773975 1101 3 0.3516346939119721 1101 4 0.4363522953793151 1101 5 0.3538155632150506 1101 6 0.3113106599191653 1101 7 0.4544925990136548 1101 8 0.4493552435464707 1101 9 0.5118053678028716 1101 10-11 0.41608610364521326 1101 12-13 0.35810396720074067 1101 14-15 0.560332434624307 1101 16-17 0.647285788046787 1101 18-19 0.6845324562100004 1101 20-21 0.6748611365961139 1101 22-23 0.6813210310201541 1101 24-25 0.6114723562583393 1101 26-27 0.4308229530149603 1101 28-29 0.5629058436585623 1101 30-31 0.6070733998787006 1101 32-33 0.5431158900769901 1101 34-35 0.6539149940797415 1101 36-37 0.749045912038703 1101 38-39 0.6209039408268211 1101 40-41 0.6686095169136905 1101 42-43 0.43529044189822486 1101 44-45 0.4344614639998454 1101 46-47 0.6813089400946595 1101 48-49 0.6075267124227821 1101 50-51 0.5861430858954506 1101 52-53 0.8749585744404627 1101 54-55 0.791263037855046 1101 56-57 0.7708403852741341 1101 58-59 0.6924103437622904 1101 60-61 0.5987308567102332 1101 62-63 0.572917190553504 1101 64-65 0.5497919831543783 1101 66-67 0.5818875117300806 1101 68-69 0.5240981720350746 1101 70-71 0.28814816897355655 1101 72-73 0.2921019088840353 1101 74-75 0.1692054210304832 1101 76-77 0.2371580916377205 1101 78-79 0.19545988277797122 1101 80-81 0.1788398432442868 1101 82-83 0.08986400703072661 1101 84-85 -0.006401031632691456 1101 86-87 0.03574035675457665 1101 88-89 0.16461697543325116 1101 90-91 0.07050342255656261 1101 92-93 0.040867154782784354 1101 94-95 0.0143014564352697 1101 96-97 -0.03821587165390028 1101 98-99 -0.18762838497738343 1101 100 0.03058381139714328 1102 1 0.4273825729621663 1102 2 0.4324550326679315 1102 3 0.43109390632908173 1102 4 0.3391649240100918 1102 5 0.42580888064028244 1102 6 0.48997761662305095 1102 7 0.36169936479102205 1102 8 0.4491732378658426 1102 9 0.36285147932657225 1102 10-11 0.45244622440616666 1102 12-13 0.5400389695664529 1102 14-15 0.4628354338728542 1102 16-17 0.3332549444925803 1102 18-19 0.47228325417856354 1102 20-21 0.3614905671710247 1102 22-23 0.48263949188929445 1102 24-25 0.48124710866469655 1102 26-27 0.40050838714061854 1102 28-29 0.3628222748530945 1102 30-31 0.2920603848245946 1102 32-33 0.32604646717781804 1102 34-35 0.21785135581182402 1102 36-37 0.2789878606493872 1102 38-39 0.19262168627081167 1102 40-41 0.3526833606832298 1102 42-43 0.2210291066430301 1102 44-45 0.30322291717421024 1102 46-47 0.36473148184610693 1102 48-49 0.20799641158972548 1102 50-51 0.25473216467570836 1102 52-53 0.20737748486730112 1102 54-55 0.3001827810402702 1102 56-57 0.2903896996583697 1102 58-59 0.3404899091752114 1102 60-61 0.1947099227610849 1102 62-63 0.26454280523466167 1102 64-65 0.10811271891142837 1102 66-67 0.11520480512616871 1102 68-69 0.17334887259215037 1102 70-71 0.11599478176361089 1102 72-73 0.05740686544437068 1102 74-75 -0.01497718669710224 1102 76-77 -0.016667506637293883 1102 78-79 -0.006132831721401999 1102 80-81 -0.06096985278791678 1102 82-83 -0.10183869456345107 1102 84-85 -0.1981147194852575 1102 86-87 -0.060745542016572784 1102 88-89 -0.20703313127993894 1102 90-91 -0.1927037079645828 1102 92-93 -0.09240861055659622 1102 94-95 -0.10340440041231957 1102 96-97 -0.17648397590910037 1102 98-99 -0.313551230681977 1102 100 0.06213671169611601 1103 1 0.37210108961624755 1103 2 0.45208335719180326 1103 3 0.37878666578876974 1103 4 0.35877646860275547 1103 5 0.3046200225145981 1103 6 0.3527051944399844 1103 7 0.3137087615524976 1103 8 0.3220610640257533 1103 9 0.21185726840283792 1103 10-11 0.3042941143298137 1103 12-13 0.31533891083669374 1103 14-15 0.4088963450231091 1103 16-17 0.4074977500970789 1103 18-19 0.2698291892402338 1103 20-21 0.27439852924837993 1103 22-23 0.3701636130403969 1103 24-25 0.3901111073223049 1103 26-27 0.35239612940087284 1103 28-29 0.40408412567951046 1103 30-31 0.4390588746307884 1103 32-33 0.4351411898894497 1103 34-35 0.4387088102962551 1103 36-37 0.4921412292205716 1103 38-39 0.35848291623977246 1103 40-41 0.3925860370822676 1103 42-43 0.37359014364277954 1103 44-45 0.4249529280811615 1103 46-47 0.5544432865277074 1103 48-49 0.5217727351068007 1103 50-51 0.4454339852982443 1103 52-53 0.4490546389909724 1103 54-55 0.6156727550313832 1103 56-57 0.5737482028885097 1103 58-59 0.5691689090913172 1103 60-61 0.5142522501375382 1103 62-63 0.45180264246933177 1103 64-65 0.42922187236389675 1103 66-67 0.6046523258913368 1103 68-69 0.4747038520367397 1103 70-71 0.4323027774008281 1103 72-73 0.4851294092819387 1103 74-75 0.3935428233708578 1103 76-77 0.4154492816838733 1103 78-79 0.13303782017892374 1103 80-81 0.15612260463300842 1103 82-83 0.23139815416546483 1103 84-85 0.08856201514013051 1103 86-87 -0.08921898777544612 1103 88-89 0.12157874891135023 1103 90-91 0.15519880063513014 1103 92-93 0.09180776665625956 1103 94-95 0.1953077398679035 1103 96-97 0.060912198266589 1103 98-99 -0.01856633262005758 1103 100 8.689878937815365E-4 1104 1 0.3665731689799898 1104 2 0.4339244048831148 1104 3 0.16285911611617365 1104 4 0.28215982582390353 1104 5 0.21563511399086366 1104 6 0.22693024572852494 1104 7 0.15527636405931133 1104 8 0.28822046598736506 1104 9 0.38387480486688474 1104 10-11 0.14485070033999392 1104 12-13 0.25300647680920463 1104 14-15 0.22403419174767691 1104 16-17 0.2474163910211118 1104 18-19 0.17721754885015883 1104 20-21 0.1367829543952226 1104 22-23 0.23700522279786895 1104 24-25 0.2598522758578383 1104 26-27 0.4093060301961984 1104 28-29 0.260754445776179 1104 30-31 0.3922907550231116 1104 32-33 0.2732844043457945 1104 34-35 0.28629614132511705 1104 36-37 0.33617068148710416 1104 38-39 0.4692823148289875 1104 40-41 0.3153244233903294 1104 42-43 0.26392049802549167 1104 44-45 0.32656184245038844 1104 46-47 0.3080587770709471 1104 48-49 0.45646978144916517 1104 50-51 0.47649657136462764 1104 52-53 0.4531232953718245 1104 54-55 0.6613047984228899 1104 56-57 0.6526531575016676 1104 58-59 0.5875660369332536 1104 60-61 0.5931663383257231 1104 62-63 0.5093048204756769 1104 64-65 0.3214007218144772 1104 66-67 0.43462520619934253 1104 68-69 0.3849749084400713 1104 70-71 0.38051821735810876 1104 72-73 0.507298001025184 1104 74-75 0.44234632504299753 1104 76-77 0.5172626476170485 1104 78-79 0.3005777971342134 1104 80-81 0.2215667774121428 1104 82-83 0.3609347306205173 1104 84-85 0.3989300279552346 1104 86-87 0.40818496917028924 1104 88-89 0.43206198528827144 1104 90-91 0.30941780971249955 1104 92-93 0.4277097423187932 1104 94-95 0.37730265316920253 1104 96-97 0.3580224756462336 1104 98-99 0.28645010781498215 1104 100 0.18874662609179893 1105 1 0.44337251778422626 1105 2 0.535531068225719 1105 3 0.43375715351787747 1105 4 0.41071241709042994 1105 5 0.4401279442294168 1105 6 0.44348437487433756 1105 7 0.5696747588978042 1105 8 0.43219690519607923 1105 9 0.6243922171446883 1105 10-11 0.565190355398471 1105 12-13 0.4659171536563136 1105 14-15 0.5154412912232331 1105 16-17 0.5219135765818663 1105 18-19 0.6458367014546411 1105 20-21 0.5953705984963094 1105 22-23 0.7131583318971835 1105 24-25 0.7300776412543541 1105 26-27 0.7093449277289849 1105 28-29 0.5809021662906488 1105 30-31 0.687609384388189 1105 32-33 0.7685295939675143 1105 34-35 0.7248853473348618 1105 36-37 0.7554019821364975 1105 38-39 0.8205292886309721 1105 40-41 0.8758784203746472 1105 42-43 0.7381302323207919 1105 44-45 0.8393807571641716 1105 46-47 0.7164310579982427 1105 48-49 0.6895718822162991 1105 50-51 0.755108785490286 1105 52-53 0.894158990991869 1105 54-55 0.7910250665905849 1105 56-57 0.9690297697218 1105 58-59 0.8545765738093181 1105 60-61 0.874224291144067 1105 62-63 0.9143347670704216 1105 64-65 0.9277554372767725 1105 66-67 0.8582291001577573 1105 68-69 0.8324031657705291 1105 70-71 0.7778625220985091 1105 72-73 0.8614878209851824 1105 74-75 0.7742504118370661 1105 76-77 0.7634427296773261 1105 78-79 0.7322315279933242 1105 80-81 0.5775190010294047 1105 82-83 0.7118558061748423 1105 84-85 0.6738327090315117 1105 86-87 0.5656921929978047 1105 88-89 0.737143294648309 1105 90-91 0.672432413558397 1105 92-93 0.6743771699153172 1105 94-95 0.5731149357785519 1105 96-97 0.5556444697229956 1105 98-99 0.5615743095895489 1105 100 0.401981699351321 1106 1 0.4179149067396466 1106 2 0.29622778774235314 1106 3 0.24177722029639526 1106 4 0.329067675181399 1106 5 0.27373813708976513 1106 6 0.33556201433980704 1106 7 0.17846054362089347 1106 8 0.3340519647032565 1106 9 0.37796370504315746 1106 10-11 0.3282262862278813 1106 12-13 0.33702362778982575 1106 14-15 0.5018996512986789 1106 16-17 0.47271085651252065 1106 18-19 0.45719663796823795 1106 20-21 0.2815836736153514 1106 22-23 0.4648091068269977 1106 24-25 0.48167480611211744 1106 26-27 0.6403040270193685 1106 28-29 0.4944517722188664 1106 30-31 0.4738448851289192 1106 32-33 0.5085588756378456 1106 34-35 0.5169898295899245 1106 36-37 0.4936366477682199 1106 38-39 0.4640557214167771 1106 40-41 0.3211816117595845 1106 42-43 0.3108336070370754 1106 44-45 0.38589825443458636 1106 46-47 0.4262956132838127 1106 48-49 0.5364415070514994 1106 50-51 0.4879998105601686 1106 52-53 0.4290417812526641 1106 54-55 0.4463510007272191 1106 56-57 0.5300771066603502 1106 58-59 0.4225182792740725 1106 60-61 0.259197463455898 1106 62-63 0.44572912729083924 1106 64-65 0.4457714417709653 1106 66-67 0.39230377521276694 1106 68-69 0.4154542282315532 1106 70-71 0.34221065382524785 1106 72-73 0.32549016774720485 1106 74-75 0.49305765569040005 1106 76-77 0.3993716336609303 1106 78-79 0.37441695390646856 1106 80-81 0.4588064808878727 1106 82-83 0.3757776623216529 1106 84-85 0.5019858118465699 1106 86-87 0.42649288585135636 1106 88-89 0.4759350142328991 1106 90-91 0.6169539172378649 1106 92-93 0.7094530696961954 1106 94-95 0.762908182117588 1106 96-97 0.525320989568538 1106 98-99 0.41253491147209687 1106 100 0.4366968637530242 1107 1 0.3365260633028413 1107 2 0.29373312054541856 1107 3 0.31815883840470605 1107 4 0.4462223605749074 1107 5 0.3400918863401188 1107 6 0.2363183908739117 1107 7 0.27653715633024234 1107 8 0.2001278826479762 1107 9 0.31130730081537905 1107 10-11 0.36679820946235253 1107 12-13 0.37823073660361217 1107 14-15 0.44914487600322417 1107 16-17 0.4328349450532585 1107 18-19 0.4025165371975419 1107 20-21 0.4847931954689386 1107 22-23 0.42713527123797945 1107 24-25 0.38937398346458707 1107 26-27 0.3405883810615862 1107 28-29 0.2705629390320823 1107 30-31 0.30749516133885635 1107 32-33 0.48042743094114826 1107 34-35 0.4890855624902031 1107 36-37 0.4723432673767505 1107 38-39 0.5183051352091255 1107 40-41 0.6125830049415697 1107 42-43 0.6137783803309844 1107 44-45 0.4953156696939871 1107 46-47 0.4911780195250657 1107 48-49 0.7104970054761637 1107 50-51 0.6237032951551384 1107 52-53 0.726578460464097 1107 54-55 0.652746599855476 1107 56-57 0.7878263972661514 1107 58-59 0.6860718736187508 1107 60-61 0.5538007748442553 1107 62-63 0.6140612039102535 1107 64-65 0.5784207947341287 1107 66-67 0.6691876067651492 1107 68-69 0.6773967066403657 1107 70-71 0.7047378303413083 1107 72-73 0.6285780685181166 1107 74-75 0.5277451961080786 1107 76-77 0.5834652509581879 1107 78-79 0.5060038462623062 1107 80-81 0.6121161360662022 1107 82-83 0.534646552707251 1107 84-85 0.5094863998246808 1107 86-87 0.4753481828539172 1107 88-89 0.6738948513210303 1107 90-91 0.599984738472763 1107 92-93 0.6322678504409254 1107 94-95 0.6609464411358275 1107 96-97 0.6945543220197479 1107 98-99 0.6181110685217028 1107 100 0.7045208005562813 1108 1 0.3062493405681721 1108 2 0.39280432797576026 1108 3 0.41606825719120977 1108 4 0.2361811872960402 1108 5 0.3650128910957875 1108 6 0.34771604814626045 1108 7 0.22009465529293237 1108 8 0.2403308764879455 1108 9 0.17968047754130367 1108 10-11 0.2840590693136207 1108 12-13 0.16453822758698067 1108 14-15 0.28546034630420536 1108 16-17 0.3521385102123702 1108 18-19 0.37746147990604584 1108 20-21 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72-73 -0.07779769126237568 2316 74-75 7.514079398021067E-4 2316 76-77 -0.03289649720017351 2316 78-79 -0.1317700605726735 2316 80-81 0.1627394890968148 2316 82-83 0.14758577441881116 2316 84-85 0.1654363579935918 2316 86-87 0.3139667871031371 2316 88-89 0.07895828098725488 2316 90-91 0.3659397042965544 2316 92-93 0.42293863090677064 2316 94-95 0.3961801801256861 2316 96-97 0.3365639803407028 2316 98-99 0.5156943372938976 2316 100 0.05606572057908821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 6.0 1 10.0 2 22.0 3 16.0 4 27.0 5 36.0 6 238.0 7 2637.0 8 4074.0 9 3284.0 10 3233.0 11 3672.0 12 4243.0 13 4656.0 14 4938.0 15 5301.0 16 6447.0 17 8131.0 18 10181.0 19 11944.0 20 13674.0 21 15553.0 22 17730.0 23 18341.0 24 18100.0 25 17939.0 26 18601.0 27 20884.0 28 23768.0 29 27480.0 30 33234.0 31 40822.0 32 51104.0 33 67188.0 34 94973.0 35 129438.0 36 192410.0 37 301290.0 38 278713.0 39 62966.0 40 89.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.521791593341526 16.54862034322106 11.553049555692562 23.37653850774485 2 17.31996672421539 19.823105295692027 40.130569851109996 22.72635812898259 3 18.758227885651753 28.176433555600237 29.623025225041115 23.442313333706892 4 12.24263775208843 16.220018352881613 37.295046304109356 34.24229759092059 5 12.290970120131206 36.16526742113423 33.121315115015875 18.42244734371869 6 28.190813493998775 34.90756289229422 21.395506394016962 15.50611721969004 7 26.812716497862045 32.5937696067318 22.925838443166178 17.66767545223998 8 27.710106491654262 32.83446538658751 21.425134877565576 18.030293244192652 9 29.078654084684523 15.744920918841471 20.565055605278314 34.61136939119569 10-11 25.44128589288927 24.825169525092484 29.18385423840741 20.549690343610838 12-13 25.656909374731217 23.574657338103727 28.641205898335535 22.127227388829517 14-15 23.459075948161452 24.491845971366395 27.21075633752732 24.838321742944835 16-17 22.57571483557332 26.216162955620348 28.058350776920683 23.149771431885647 18-19 22.032173928518933 25.673591368960714 29.86990559141642 22.424329111103933 20-21 23.220849483709777 25.444863842480647 29.535867760097002 21.79841891371258 22-23 22.598107555438048 25.17393670731261 29.705669916342252 22.522285820907083 24-25 21.95163669020293 25.367684740428263 29.756766331886002 22.923912237482803 26-27 21.837193706304074 25.12729591542496 29.779516323305756 23.255994054965207 28-29 22.109128519652838 26.021370062400212 29.50470078089259 22.364800637054362 30-31 23.73211367886255 25.438107347258114 29.658946274958787 21.17083269892054 32-33 22.999445972789058 25.348837745831037 29.755413959837806 21.896302321542098 34-35 22.934641104486563 25.722322743946453 29.397693603192966 21.94534254837402 36-37 22.84608866490472 25.95095221286538 29.32274451138262 21.880214610847283 38-39 22.951724656161314 26.49760282697344 28.593707927528815 21.95696458933643 40-41 22.910246412513043 25.62789673627513 29.240367064401067 22.221489786810757 42-43 22.38730009720524 26.1395411081925 28.757094680674683 22.716064113927576 44-45 22.0381461401764 26.477947844328725 28.922693429595842 22.56121258589903 46-47 22.0053003853837 25.470358941096244 28.887891345065896 23.636449328454155 48-49 22.07667788486255 25.76899348659155 29.04253818602695 23.111790442518938 50-51 22.160284418103576 26.47799931848276 28.89095921903661 22.470757044377056 52-53 22.434401208123855 26.607794139070034 27.921055354985256 23.036749297820855 54-55 21.98053995189866 26.35886949651798 28.789249610499105 22.871340941084263 56-57 22.299951057858838 26.48158737325716 27.993454606576044 23.22500696230796 58-59 22.394782561118586 26.827344553142918 28.246504013376494 22.531368872362005 60-61 23.32652821760901 26.76193608948211 28.28903306271078 21.622502630198102 62-63 23.19342985077973 26.495713815683196 28.32257583819778 21.9882804953393 64-65 22.69044283665969 25.954150666644694 28.159895841917038 23.19551065477858 66-67 22.349290198700857 26.289821990212115 28.163224142175807 23.19766366891122 68-69 21.95053137701862 26.335660730836214 28.490008582717756 23.22379930942741 70-71 22.100703629899574 26.107949254257797 28.70127622541869 23.09007089042394 72-73 21.74544797671189 27.188262591840655 27.732886874594342 23.333402556853112 74-75 22.328500695557004 26.748617127184744 27.775898784917953 23.146983392340303 76-77 21.95987233184229 26.291677707773864 28.24984278781695 23.498607172566903 78-79 21.88266032847093 26.63096817653615 28.25901970205998 23.227351792932936 80-81 22.26498411068272 26.729549384103564 27.566169234247624 23.439297270966087 82-83 22.664950696055687 26.92750079097237 27.68835029529635 22.719198217675597 84-85 22.509627759586976 27.50311038018321 26.948432780358544 23.038829079871267 86-87 22.13604974924986 27.23176853995391 27.413700818958926 23.218480891837306 88-89 21.640186460027895 26.751622165330353 28.080070766220995 23.528120608420757 90-91 22.948950109000393 26.69105524378579 27.742824992237804 22.617169654976017 92-93 22.493541491017417 26.907344868888476 27.634376209219436 22.96473743087467 94-95 22.237987042306624 26.57190636553773 27.446573738819886 23.743532853335758 96-97 22.2137781937542 26.67817925361526 27.529495511409323 23.578547041221213 98-99 22.227755386953323 26.66934499670262 27.336961053368086 23.765938562975965 100 22.14642398925067 26.91261424127705 27.400105225265143 23.540856544207138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 975.0 1 774.5 2 2125.5 3 3075.0 4 1729.0 5 1048.0 6 1324.5 7 2407.0 8 3791.5 9 4165.5 10 3305.0 11 2512.0 12 2491.0 13 2660.5 14 2851.5 15 2993.5 16 3094.0 17 3292.0 18 3510.5 19 3638.0 20 3849.0 21 4092.0 22 4469.0 23 5115.5 24 5965.0 25 6879.5 26 8082.0 27 9474.5 28 10994.0 29 12991.0 30 15303.5 31 17622.5 32 20034.0 33 22706.0 34 25571.0 35 28218.5 36 31592.0 37 34728.0 38 36678.5 39 38555.5 40 39128.0 41 39323.5 42 39924.5 43 40896.0 44 42544.5 45 44884.5 46 47137.5 47 49237.0 48 53350.0 49 57919.5 50 63117.5 51 64665.5 52 60768.0 53 57700.5 54 55859.5 55 52703.0 56 49045.5 57 46743.5 58 43568.5 59 38399.5 60 30432.0 61 22388.0 62 17708.0 63 13942.5 64 10275.0 65 7733.0 66 6010.5 67 4777.5 68 3808.5 69 3038.0 70 2494.0 71 2050.5 72 1696.5 73 1493.5 74 1325.0 75 1120.5 76 952.5 77 770.0 78 672.0 79 601.0 80 500.0 81 432.5 82 404.0 83 366.5 84 289.5 85 226.5 86 174.5 87 134.0 88 105.5 89 71.5 90 51.5 91 40.0 92 29.0 93 27.0 94 25.0 95 17.5 96 13.0 97 10.5 98 9.5 99 13.5 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16152704012737637 2 0.10386234811943906 3 0.029524322308063896 4 0.030644664895646677 5 0.02537246448349241 6 0.0024383926906213485 7 0.0037564427936599156 8 0.005997127968825479 9 0.005997127968825479 10-11 0.004349565340027271 12-13 0.0077435443553515795 14-15 0.017101700086925405 16-17 0.007974203123383329 18-19 0.017398261360109083 20-21 0.017892530148748545 22-23 0.044648947240431454 24-25 0.015190527437519483 26-27 0.010577352076884499 28-29 0.027085929617442547 30-31 0.015948406246766657 32-33 0.021055850396041104 34-35 0.020297971586793927 36-37 0.02118765540634496 38-39 0.019342385262090967 40-41 0.025306561978340483 42-43 0.03291830132338821 44-45 0.03746557417887126 46-47 0.021978485468168104 48-49 0.014169038607664592 50-51 0.012521475978866384 52-53 0.021747826700136353 54-55 0.025537220746372234 56-57 0.019474190272394824 58-59 0.026591660828803085 60-61 0.026163294545315553 62-63 0.051140343997896395 64-65 0.03084237241110246 66-67 0.020660435365129536 68-69 0.02576787951440398 70-71 0.0405629919210119 72-73 0.03739967167371933 74-75 0.04105726070965136 76-77 0.022077339225895994 78-79 0.015948406246766657 80-81 0.02214324173104792 82-83 0.017991383906476435 84-85 0.019474190272394824 86-87 0.022736364277415277 88-89 0.01878221396829958 90-91 0.028074467194721474 92-93 0.02649280707107519 94-95 0.01990255655588236 96-97 0.014037233597360737 98-99 0.01835384768481204 100 0.042638920833297636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1517393.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.6530005215084 #Duplication Level Percentage of deduplicated Percentage of total 1 82.21289545226279 53.15310372549862 2 11.23401866861635 14.526260296813762 3 2.8955360593826165 5.616152830719321 4 1.1676054916736347 3.0195679384836636 5 0.6484422459432794 2.0961868432569464 6 0.41053851006068565 1.5925527903031675 7 0.26617123677487875 1.2046138377011741 8 0.20426091549641365 1.0564864860890735 9 0.1449509335251846 0.84343615027172 >10 0.7391646574641132 8.490773236659052 >50 0.04979442350409396 2.225977412695548 >100 0.023859883872233034 2.9363039646284324 >500 0.0015464519973247886 0.6428177420482393 >1k 8.836868556141649E-4 1.1321619447495541 >5k 3.313825708553118E-4 1.4636048000818371 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8196 0.5401369322252046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6919 0.4559794331461922 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5379 0.3544895752122225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4953 0.32641510801750107 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2655 0.17497115117836973 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1610 0.10610303329460463 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.590250515192834E-5 7 0.0 0.0 0.0 0.0 6.590250515192834E-5 8 0.0 0.0 0.0 0.0 1.3180501030385667E-4 9 0.0 0.0 0.0 6.590250515192834E-5 1.3180501030385667E-4 10-11 0.0 0.0 0.0 6.590250515192834E-5 1.3180501030385667E-4 12-13 6.590250515192834E-5 0.0 0.0 6.590250515192834E-5 1.3180501030385667E-4 14-15 6.590250515192834E-5 0.0 0.0 6.590250515192834E-5 2.306587680317492E-4 16-17 6.590250515192834E-5 0.0 0.0 6.590250515192834E-5 2.6361002060771335E-4 18-19 6.590250515192834E-5 0.0 0.0 1.3180501030385667E-4 2.6361002060771335E-4 20-21 6.590250515192834E-5 0.0 0.0 1.9770751545578503E-4 3.2951252575964173E-4 22-23 6.590250515192834E-5 0.0 0.0 3.6246377833560586E-4 3.2951252575964173E-4 24-25 6.590250515192834E-5 0.0 0.0 6.919763040952476E-4 3.2951252575964173E-4 26-27 6.590250515192834E-5 0.0 0.0 9.226350721269967E-4 3.2951252575964173E-4 28-29 6.590250515192834E-5 0.0 0.0 0.0021747826700136354 3.2951252575964173E-4 30-31 6.590250515192834E-5 0.0 0.0 0.006722055525496691 3.2951252575964173E-4 32-33 6.590250515192834E-5 0.0 0.0 0.017332358854957153 3.2951252575964173E-4 34-35 6.590250515192834E-5 0.0 0.0 0.03268764255535646 3.6246377833560586E-4 36-37 6.590250515192834E-5 0.0 0.0 0.05397415171942931 3.9541503091157005E-4 38-39 6.590250515192834E-5 0.0 0.0 0.09269187349618721 3.9541503091157005E-4 40-41 6.590250515192834E-5 0.0 0.0 0.1542118620555123 3.9541503091157005E-4 42-43 6.590250515192834E-5 3.295125257596417E-5 0.0 0.21042669895010718 3.9541503091157005E-4 44-45 6.590250515192834E-5 6.590250515192834E-5 0.0 0.2706945399115457 3.9541503091157005E-4 46-47 6.590250515192834E-5 6.590250515192834E-5 0.0 0.3398592190684945 3.9541503091157005E-4 48-49 6.590250515192834E-5 6.590250515192834E-5 0.0 0.412912146029407 5.931225463673551E-4 50-51 6.590250515192834E-5 6.590250515192834E-5 0.0 0.5037257981287643 5.931225463673551E-4 52-53 6.590250515192834E-5 6.590250515192834E-5 0.0 0.6038646547071194 5.931225463673551E-4 54-55 6.590250515192834E-5 6.590250515192834E-5 0.0 0.7247298491557559 5.931225463673551E-4 56-57 6.590250515192834E-5 6.590250515192834E-5 0.0 0.8461222636456079 5.931225463673551E-4 58-59 6.590250515192834E-5 6.590250515192834E-5 0.0 0.9857696720625441 5.931225463673551E-4 60-61 6.590250515192834E-5 6.590250515192834E-5 0.0 1.1371147751439477 5.931225463673551E-4 62-63 6.590250515192834E-5 6.590250515192834E-5 0.0 1.3128438051315645 5.931225463673551E-4 64-65 6.590250515192834E-5 6.590250515192834E-5 0.0 1.4829052196761157 5.931225463673551E-4 66-67 6.590250515192834E-5 6.590250515192834E-5 0.0 1.6700024318024402 5.931225463673551E-4 68-69 6.590250515192834E-5 6.590250515192834E-5 0.0 1.9097886967977313 5.931225463673551E-4 70-71 6.590250515192834E-5 6.590250515192834E-5 0.0 2.1773199164619843 5.931225463673551E-4 72-73 6.590250515192834E-5 6.590250515192834E-5 0.0 2.4097910033854117 6.590250515192835E-4 74-75 6.590250515192834E-5 6.590250515192834E-5 0.0 2.6572878614834785 6.590250515192835E-4 76-77 6.590250515192834E-5 6.590250515192834E-5 0.0 2.9251156424209155 7.249275566712117E-4 78-79 1.3180501030385667E-4 6.590250515192834E-5 0.0 3.2438531085882167 7.249275566712117E-4 80-81 1.3180501030385667E-4 6.590250515192834E-5 0.0 3.545060508385105 7.578788092471759E-4 82-83 1.3180501030385667E-4 6.590250515192834E-5 0.0 3.8824483835104022 8.567325669750684E-4 84-85 1.3180501030385667E-4 6.590250515192834E-5 0.0 4.239343400160671 8.567325669750684E-4 86-87 1.3180501030385667E-4 6.590250515192834E-5 0.0 4.617524925975012 8.567325669750684E-4 88 1.3180501030385667E-4 6.590250515192834E-5 0.0 4.919028887045084 8.567325669750684E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 320 0.0 66.07618 4 TACCTGG 2520 0.0 64.93031 2 GTACCTG 2870 0.0 57.901806 1 TATAACG 350 0.0 57.7656 2 ACCTGGG 2865 0.0 55.761784 3 ATAACGC 380 0.0 51.93356 3 CCTGGGG 2505 0.0 40.891323 4 GTATAAC 570 0.0 39.64292 1 GGTATCA 5080 0.0 36.697014 1 TATCACG 195 0.0 36.168083 2 GTACCCG 365 0.0 33.533566 1 CTGGGGG 1755 0.0 32.39381 5 TGGGGGG 3775 0.0 30.98491 6 ATGGGGG 2900 0.0 30.458858 5 ATATCTA 540 0.0 29.640425 1 CATGGGG 4130 0.0 29.46674 4 GTATCAA 7470 0.0 29.430357 1 GTGGTTA 1280 0.0 29.42248 1 GACCGTA 80 4.7955193E-4 29.360415 7 CCCTAGA 695 0.0 29.126137 1 >>END_MODULE