FastQCFastQC Report
Mon 6 Jun 2016
ERR1211555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517393
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86500.5700566695641802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.4987501589897937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56520.372480959118699No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50840.33504833619240365No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24510.16152704012737637No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19170.12633510237624662No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.10537810573793341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3450.070.88472
TAACGCA3600.066.5721054
TACCTGG28350.062.8715822
GTACCTG31250.057.6428761
ACCTGGG31950.055.007943
ATAACGC4750.051.443963
GTATAAC5850.049.0423051
CCTGGGG25800.045.899244
TATCACG1900.039.6035962
TACCGTA952.122033E-839.5696687
CTGGGGG16400.038.395965
GTACCCG3500.034.9383351
TGGGGGG35750.034.7006956
ATGGGGG30850.031.9881235
GGTATCA56250.031.8565941
TATCTAA5950.030.8259072
CATGGGG42350.030.292414
ATATCTA6150.029.8254051
ATCACGC2850.028.0303613
ACCGTAT1205.855467E-627.4102368
Produced by FastQC (version 0.11.5)