##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211555_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1517393 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07533578973938 33.0 31.0 34.0 30.0 34.0 2 32.26767422810043 33.0 31.0 34.0 30.0 34.0 3 32.269773881914574 34.0 31.0 34.0 30.0 34.0 4 35.93720677504114 37.0 35.0 37.0 35.0 37.0 5 35.876509249746114 37.0 35.0 37.0 35.0 37.0 6 35.90106056901541 37.0 35.0 37.0 35.0 37.0 7 35.83785413534925 37.0 35.0 37.0 35.0 37.0 8 35.820241690847396 37.0 35.0 37.0 35.0 37.0 9 37.46148624647669 39.0 37.0 39.0 34.0 39.0 10-11 37.39950526989382 39.0 37.0 39.0 34.0 39.0 12-13 37.29829978126959 39.0 37.0 39.0 34.0 39.0 14-15 38.478564880686804 40.0 38.0 41.0 34.0 41.0 16-17 38.39396945946106 40.0 38.0 41.0 34.0 41.0 18-19 38.394228126793784 40.0 38.0 41.0 33.5 41.0 20-21 38.32669519366439 40.0 38.0 41.0 34.0 41.0 22-23 38.18888349952847 40.0 37.5 41.0 33.5 41.0 24-25 38.06536276363474 40.0 37.0 41.0 33.0 41.0 26-27 37.86904677957523 40.0 37.0 41.0 33.0 41.0 28-29 37.880229775674465 40.0 37.0 41.0 33.0 41.0 30-31 37.73764575162795 40.0 37.0 41.0 33.0 41.0 32-33 37.446317466865864 40.0 36.0 41.0 32.0 41.0 34-35 37.25918532641181 39.0 36.0 41.0 31.5 41.0 36-37 37.162935376662475 39.5 36.0 41.0 31.0 41.0 38-39 37.03738649117269 39.0 35.5 41.0 31.0 41.0 40-41 36.844964356630086 39.0 35.0 41.0 30.5 41.0 42-43 36.68299544020567 39.0 35.0 41.0 30.0 41.0 44-45 36.46837767144042 39.0 35.0 41.0 30.0 41.0 46-47 36.152269056203636 39.0 35.0 41.0 28.5 41.0 48-49 35.88164305489745 39.0 35.0 40.0 27.0 41.0 50-51 35.65118825511914 38.5 35.0 40.0 26.5 41.0 52-53 35.445377697142405 38.0 34.0 40.0 26.0 41.0 54-55 35.22818379945077 38.0 34.0 40.0 26.0 41.0 56-57 35.01639127108139 38.0 34.0 40.0 25.5 41.0 58-59 34.74498399557662 38.0 33.5 40.0 25.0 41.0 60-61 34.381756736718835 37.0 33.0 40.0 24.0 41.0 62-63 33.98334940256084 36.5 32.5 39.5 23.0 41.0 64-65 33.66868042754909 36.0 32.0 39.0 22.5 41.0 66-67 33.329223872787075 35.5 32.0 39.0 22.0 40.0 68-69 32.93315673658702 35.0 31.0 38.5 21.5 40.0 70-71 32.5361228106364 35.0 31.0 37.5 20.5 40.0 72-73 32.120442100365565 35.0 31.0 37.0 20.0 39.0 74-75 31.711496955633777 34.5 30.0 36.5 19.5 39.0 76-77 30.193398480156425 33.0 28.5 35.0 18.5 37.0 78-79 31.05278527052649 34.0 30.0 35.0 19.0 37.5 80-81 31.061706163136378 34.0 30.0 35.0 19.0 37.0 82-83 30.787673331826362 34.0 30.0 35.0 18.5 37.0 84-85 30.495750276955278 34.0 29.0 35.0 18.0 36.0 86-87 30.212791280834956 34.0 29.0 35.0 18.0 36.0 88-89 29.975445385605443 34.0 29.0 35.0 17.0 35.5 90-91 29.676007468071884 34.0 29.0 35.0 15.0 35.0 92-93 29.448035874687704 34.0 28.5 35.0 14.5 35.0 94-95 29.244288394634744 33.0 27.0 35.0 12.0 35.0 96-97 29.047485061549644 33.0 27.0 35.0 11.0 35.0 98-99 28.86605216974113 33.0 27.0 35.0 10.0 35.0 100 27.0659558861811 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37452715980559503 1101 2 0.23259945105822055 1101 3 0.342912298694138 1101 4 0.1739567734595937 1101 5 0.1822449182361865 1101 6 0.09930201858898613 1101 7 0.24963168622632992 1101 8 0.22114507849066456 1101 9 0.15800611688282373 1101 10-11 0.17446973794022114 1101 12-13 0.1579937322807652 1101 14-15 0.26041039205941985 1101 16-17 0.2249857473057162 1101 18-19 0.2601989738215664 1101 20-21 0.21954064502511272 1101 22-23 0.21947683230761328 1101 24-25 0.08144327977316124 1101 26-27 0.0860008777759731 1101 28-29 0.15076204551895955 1101 30-31 0.28239167243561525 1101 32-33 0.2931266172129341 1101 34-35 0.40265622824109926 1101 36-37 0.26802664786554686 1101 38-39 0.36131031186909013 1101 40-41 0.4351289211432956 1101 42-43 0.39871915235841726 1101 44-45 0.3838908345024592 1101 46-47 0.6958954585845376 1101 48-49 0.5818299027883143 1101 50-51 0.4821930396726444 1101 52-53 0.5452729346359106 1101 54-55 0.5119114904637385 1101 56-57 0.48630547668883395 1101 58-59 0.476764198532301 1101 60-61 0.3937677501123389 1101 62-63 0.28148247394943837 1101 64-65 0.366959582132246 1101 66-67 0.3518599406111278 1101 68-69 0.28979494567401076 1101 70-71 0.33544372824893287 1101 72-73 0.026883193139852324 1101 74-75 0.11376695642351464 1101 76-77 0.2094660640223367 1101 78-79 0.3835830043458408 1101 80-81 0.3405028678135942 1101 82-83 0.4198097441812365 1101 84-85 0.30980326508691647 1101 86-87 0.1697516611524854 1101 88-89 0.14636165119929245 1101 90-91 -0.01902804269333558 1101 92-93 -0.012518906424908494 1101 94-95 0.13405962921746806 1101 96-97 0.05616416189496931 1101 98-99 -0.13403495739124693 1101 100 -0.051213644011603776 1102 1 0.27703766633069904 1102 2 0.27911673390750025 1102 3 0.33032497625038104 1102 4 0.23231724285114552 1102 5 0.16465042542778718 1102 6 0.22524555508020683 1102 7 0.11308970626819104 1102 8 0.2215061601830186 1102 9 0.16670833215196268 1102 10-11 0.1988647246908002 1102 12-13 0.28582251071137676 1102 14-15 0.3030714982145142 1102 16-17 0.2752372593810719 1102 18-19 0.2212803365863536 1102 20-21 0.3318145295170609 1102 22-23 0.210731038213531 1102 24-25 0.15072866761006054 1102 26-27 0.16518030681280038 1102 28-29 0.23461118368360445 1102 30-31 0.3707032456531678 1102 32-33 0.3603293766680764 1102 34-35 0.3698825715309155 1102 36-37 0.3524564097859937 1102 38-39 0.4150649755357776 1102 40-41 0.3121643086139798 1102 42-43 0.3348065941861833 1102 44-45 0.3948342464827519 1102 46-47 0.4882707389172296 1102 48-49 0.4730375635795312 1102 50-51 0.3961750309978882 1102 52-53 0.5346979285202238 1102 54-55 0.5053358486098958 1102 56-57 0.43913945528174736 1102 58-59 0.6159258518030555 1102 60-61 0.5409405829996317 1102 62-63 0.5646135502658467 1102 64-65 0.44226489025285076 1102 66-67 0.43355668765326527 1102 68-69 0.4399886334093921 1102 70-71 0.4932796404233173 1102 72-73 0.6143825156539151 1102 74-75 0.34183563811645357 1102 76-77 0.2522749353527409 1102 78-79 0.4272024784409787 1102 80-81 0.43587439377558823 1102 82-83 0.31027729736571175 1102 84-85 0.17727126944039995 1102 86-87 0.38315826550509513 1102 88-89 0.4293107218350727 1102 90-91 0.1464413049133455 1102 92-93 0.06821050056616329 1102 94-95 0.11072902780038874 1102 96-97 -0.18525282373940044 1102 98-99 0.09231638901134076 1102 100 -0.027659106027716263 1103 1 0.27802768227163455 1103 2 0.28640341903209077 1103 3 0.38550158502629017 1103 4 0.2141655522730801 1103 5 0.19719090701181585 1103 6 0.20318833551480964 1103 7 0.17716806854690503 1103 8 0.20346773414055974 1103 9 0.2276068974678438 1103 10-11 0.2947934938828425 1103 12-13 0.33698661843853017 1103 14-15 0.32178112150505456 1103 16-17 0.34692741795142723 1103 18-19 0.3382448470318664 1103 20-21 0.30391579640186706 1103 22-23 0.46587198963563026 1103 24-25 0.374445002873081 1103 26-27 0.3379276010488965 1103 28-29 0.4392004948674284 1103 30-31 0.45891114900076246 1103 32-33 0.5089303499040909 1103 34-35 0.5657735858777571 1103 36-37 0.5476615444449422 1103 38-39 0.5331852037428391 1103 40-41 0.618450490340642 1103 42-43 0.4339864750486697 1103 44-45 0.40457080156287617 1103 46-47 0.534214190549072 1103 48-49 0.5904341572534975 1103 50-51 0.6325539218444405 1103 52-53 0.49706264032490566 1103 54-55 0.4368275844937841 1103 56-57 0.5542309059067989 1103 58-59 0.5971239388908955 1103 60-61 0.6149232996704939 1103 62-63 0.6836315047922383 1103 64-65 0.5658658634888596 1103 66-67 0.6584371306434491 1103 68-69 0.6846882726408765 1103 70-71 0.5996392343489845 1103 72-73 0.5323915768167637 1103 74-75 0.4374434911242595 1103 76-77 0.4232813117266012 1103 78-79 0.5301305764357771 1103 80-81 0.6275389629508545 1103 82-83 0.5770320586711897 1103 84-85 0.42343495766928285 1103 86-87 0.3384502363080166 1103 88-89 0.5703544335558526 1103 90-91 0.49968402661818345 1103 92-93 0.5951108277748212 1103 94-95 0.5681226849863954 1103 96-97 0.4621064345876391 1103 98-99 0.3645246279575609 1103 100 0.32308760091078526 1104 1 0.20237779327970173 1104 2 0.2554982114891615 1104 3 0.2647683697268448 1104 4 -0.023561733228426363 1104 5 0.10802754248381063 1104 6 0.13052161264152318 1104 7 0.16298292395538994 1104 8 0.09942928881554991 1104 9 0.06519226126516742 1104 10-11 0.1283973215533578 1104 12-13 0.18870935120417442 1104 14-15 0.23050860372971016 1104 16-17 0.09882930930403688 1104 18-19 0.19736437039976096 1104 20-21 0.12317780354010921 1104 22-23 0.2850257990389977 1104 24-25 0.37700380904456665 1104 26-27 0.3239476106040229 1104 28-29 0.4108547960786879 1104 30-31 0.3791342892643783 1104 32-33 0.35752448193342445 1104 34-35 0.43649064177523655 1104 36-37 0.39531210791626137 1104 38-39 0.4900431134188139 1104 40-41 0.5149741111708082 1104 42-43 0.6111883692116109 1104 44-45 0.5046579218169356 1104 46-47 0.5429803148219037 1104 48-49 0.4233599926243201 1104 50-51 0.30910803476352555 1104 52-53 0.43200769835824104 1104 54-55 0.49322319475711396 1104 56-57 0.6644471608600071 1104 58-59 0.6571195828781953 1104 60-61 0.4882342908741606 1104 62-63 0.4169163599196821 1104 64-65 0.6606295146706032 1104 66-67 0.5939941382032359 1104 68-69 0.6580877892639805 1104 70-71 0.5055521703015415 1104 72-73 0.5775887215206978 1104 74-75 0.83687650689021 1104 76-77 0.6496337834125185 1104 78-79 0.7155533907010145 1104 80-81 0.8722794289036955 1104 82-83 0.6741142327430794 1104 84-85 0.7395255065268813 1104 86-87 0.6678104645068714 1104 88-89 0.5268343537424585 1104 90-91 0.488910623588648 1104 92-93 0.31167491736816544 1104 94-95 0.6229353764401786 1104 96-97 0.5314899885319413 1104 98-99 0.7621084460729506 1104 100 0.787168763263594 1105 1 0.1804239657024027 1105 2 0.1345959689487728 1105 3 0.22190899557163135 1105 4 0.11669315824895676 1105 5 0.16264178108338 1105 6 0.17430161180049453 1105 7 0.26557779488766897 1105 8 0.17271284131670228 1105 9 0.1991649759224785 1105 10-11 0.20527176211972886 1105 12-13 0.2392904112595673 1105 14-15 0.307416835589045 1105 16-17 0.28770236217464884 1105 18-19 0.3261617608108409 1105 20-21 0.22913678541595317 1105 22-23 0.39782714228044114 1105 24-25 0.45470425899440414 1105 26-27 0.2434890402310259 1105 28-29 0.4024054710034406 1105 30-31 0.38979807040492886 1105 32-33 0.37557654155028075 1105 34-35 0.40670173973694546 1105 36-37 0.3900631937431527 1105 38-39 0.6050701764898818 1105 40-41 0.4390645997478515 1105 42-43 0.3604952722471211 1105 44-45 0.5304872352379348 1105 46-47 0.478141581890263 1105 48-49 0.4346192514385834 1105 50-51 0.5445882916374316 1105 52-53 0.46282911212586697 1105 54-55 0.3200578366895499 1105 56-57 0.33484388565771184 1105 58-59 0.42506951769854595 1105 60-61 0.30601737179905086 1105 62-63 0.36604769841532203 1105 64-65 0.5067544793774701 1105 66-67 0.4722245858102099 1105 68-69 0.43852698596234774 1105 70-71 0.6079330297117451 1105 72-73 0.5478043249881352 1105 74-75 0.6491290795339815 1105 76-77 0.5628757651141569 1105 78-79 0.5475858844083241 1105 80-81 0.3400004840330624 1105 82-83 0.25620755077772017 1105 84-85 0.33986086633656853 1105 86-87 0.38707840993618703 1105 88-89 0.24601669456294317 1105 90-91 0.46742386677181713 1105 92-93 0.5148416871608532 1105 94-95 0.3861830651157412 1105 96-97 0.4180533689828536 1105 98-99 0.45291811548898053 1105 100 0.5332234420811091 1106 1 0.29859264564121446 1106 2 0.17294906753543415 1106 3 0.19700749464062284 1106 4 0.14561579211935083 1106 5 0.09936188667256829 1106 6 0.09752104009060503 1106 7 0.12243437208843488 1106 8 0.1333683339328644 1106 9 0.1694461192214476 1106 10-11 0.1580042901442127 1106 12-13 0.17753953650930754 1106 14-15 0.19204512902188497 1106 16-17 0.15751615400748875 1106 18-19 0.10625645431979791 1106 20-21 0.15479358430570045 1106 22-23 0.16891987411379716 1106 24-25 0.1286530862929638 1106 26-27 0.2607654522523717 1106 28-29 0.34602692952963565 1106 30-31 0.5432204694481015 1106 32-33 0.5635400610288599 1106 34-35 0.5595340504518589 1106 36-37 0.5760199221092179 1106 38-39 0.5354008675765627 1106 40-41 0.7188980385394999 1106 42-43 0.6318817268695938 1106 44-45 0.7326812085597467 1106 46-47 0.6727423680706366 1106 48-49 0.7070854582164117 1106 50-51 0.869514974200861 1106 52-53 1.01621908085081 1106 54-55 0.8142343482293128 1106 56-57 0.7757450105631349 1106 58-59 0.8689359969061385 1106 60-61 1.0172491726897306 1106 62-63 0.9796600885335565 1106 64-65 1.16450343775643 1106 66-67 0.9837707606938153 1106 68-69 0.8035237995608853 1106 70-71 1.0422442686480622 1106 72-73 0.9375763538197859 1106 74-75 0.9164036038767343 1106 76-77 0.9456403534718625 1106 78-79 1.0126062562357028 1106 80-81 1.0648922822960962 1106 82-83 1.0650816033518318 1106 84-85 1.0653255750526611 1106 86-87 0.8859046513868947 1106 88-89 1.0712765523980892 1106 90-91 1.0304883770551356 1106 92-93 0.9505654759885154 1106 94-95 1.1766465883696746 1106 96-97 1.2618930809079956 1106 98-99 1.4902694230461861 1106 100 1.570450351619023 1107 1 0.2025772176919176 1107 2 0.15193355572238687 1107 3 0.2290706585853215 1107 4 0.19843989478648183 1107 5 0.16875709480936507 1107 6 0.14056103788406205 1107 7 0.13748884812073214 1107 8 0.18198135786235525 1107 9 0.267330031655753 1107 10-11 0.19071003682422827 1107 12-13 0.2097829143475778 1107 14-15 0.3116042814835893 1107 16-17 0.28168184940761165 1107 18-19 0.17516394812412983 1107 20-21 0.28770813310192267 1107 22-23 0.2771932013766403 1107 24-25 0.33717267481637236 1107 26-27 0.44616753050806324 1107 28-29 0.2358109449486392 1107 30-31 0.18059060642184477 1107 32-33 0.20629238750262857 1107 34-35 0.2573254145722643 1107 36-37 0.3606007691927928 1107 38-39 0.34363371659981823 1107 40-41 0.26679710235907095 1107 42-43 0.3259525220433659 1107 44-45 0.5057076800611711 1107 46-47 0.30080679630805207 1107 48-49 0.4859516425981454 1107 50-51 0.5189545965828728 1107 52-53 0.6749727983447471 1107 54-55 0.6008165572602806 1107 56-57 0.721885283690618 1107 58-59 0.7676492659787186 1107 60-61 0.6788755033745915 1107 62-63 0.7051656285732761 1107 64-65 0.6560518347845914 1107 66-67 0.6987266129489882 1107 68-69 0.7785232684237613 1107 70-71 0.7706910037947665 1107 72-73 0.7610602927923154 1107 74-75 0.956910357145464 1107 76-77 0.9155636423623754 1107 78-79 0.9239648751748248 1107 80-81 0.8928336730851747 1107 82-83 0.9340124299167378 1107 84-85 0.7052625020535466 1107 86-87 0.6894457891683352 1107 88-89 0.8453777845243948 1107 90-91 0.7556439256862362 1107 92-93 0.6871432681950012 1107 94-95 0.846906413195061 1107 96-97 0.9969063481542051 1107 98-99 1.1723346556301983 1107 100 1.3131313752637546 1108 1 0.25310864024466184 1108 2 0.24494048177007244 1108 3 0.2202423415829955 1108 4 0.15058952295027694 1108 5 0.17847271192979264 1108 6 0.20155494168540855 1108 7 0.151257958586406 1108 8 0.19799106071257455 1108 9 0.19446928670666352 1108 10-11 0.2561978923028292 1108 12-13 0.3067252398399063 1108 14-15 0.2713629869190939 1108 16-17 0.2721800779991028 1108 18-19 0.36286941555255936 1108 20-21 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quality scores pass #Quality Count 9 1.0 10 6.0 11 8.0 12 23.0 13 68.0 14 258.0 15 699.0 16 1662.0 17 3564.0 18 6583.0 19 9479.0 20 11767.0 21 14402.0 22 17459.0 23 19306.0 24 19687.0 25 19953.0 26 22338.0 27 25922.0 28 30549.0 29 36357.0 30 43757.0 31 52299.0 32 63536.0 33 79943.0 34 107703.0 35 141825.0 36 200854.0 37 298064.0 38 254768.0 39 34546.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.342738100808575 17.815785536767695 12.58858365664899 23.252892705774737 2 16.64944405133944 20.754317578871774 39.72640770975266 22.869830660036126 3 17.768223316064567 28.950568182192633 30.56420165508642 22.717006846656382 4 11.829271507466817 17.19088165126732 37.22903952866125 33.75080731260462 5 11.794587227670963 36.74499497157589 33.41555872184445 18.044859078908694 6 27.971345439687354 35.72593639715695 21.236271612016726 15.066446551138965 7 26.580852817958167 32.99362788677686 23.028839595279536 17.396679699985434 8 27.71719467003387 33.364681164874 21.274457404623618 17.64366676046852 9 28.876209230185236 15.765613477343676 20.85533656124229 34.5028407312288 10-11 25.366698014291618 25.23397036891563 29.173160809361846 20.2261708074309 12-13 25.513396990759812 23.820328682154194 28.89475567634753 21.771518650738468 14-15 23.30404845679399 24.708331987823854 27.48875868018371 24.49886087519845 16-17 22.501468818055372 26.44607320595778 28.28533327094495 22.767124705041894 18-19 21.951231567674437 25.770656561496004 30.2812093253151 21.99690254551446 20-21 23.083726483097553 25.63127764089711 29.93535724865793 21.349638627347407 22-23 22.547257038881817 25.258650857095034 30.114380387941686 22.079711716081462 24-25 21.851597394873114 25.41550719408459 30.188497702473153 22.544397708569143 26-27 21.735326710344694 25.230041159450277 30.206970317152827 22.827661813052202 28-29 22.11807713422008 26.105012518279853 29.856542292278604 21.920368055221466 30-31 23.47488431452215 25.53713880313187 30.230164920386844 20.757811961959142 32-33 22.874449368239848 25.454180583491727 30.200191386550745 21.47117866171768 34-35 22.916109907064122 25.83507349885803 29.749959880356137 21.498856713721707 36-37 22.73143962060721 26.110110111429496 29.799317504211224 21.35913276375207 38-39 22.960241153625375 26.550261537538894 29.01445458697367 21.47504272186206 40-41 22.86616630945696 25.801250365777467 29.56318921042156 21.769394114344014 42-43 22.316135727111643 26.371413064503958 29.04096732704165 22.271483881342746 44-45 22.055250082546912 26.614739550483453 29.226418439365748 22.103591927603887 46-47 21.9665469136071 25.649464274769013 29.125499492845464 23.258489318778427 48-49 22.0070014667951 25.984023104906335 29.296060282609332 22.712915145689234 50-51 22.16774920663167 26.723479418559055 29.044959186430848 22.06381218837843 52-53 22.318634077028577 26.744360005351496 28.092383148590322 22.844622769029606 54-55 21.876449886645226 26.371724574260558 29.073753097485106 22.67807244160911 56-57 22.33636437029507 26.578410163722126 28.136810211173042 22.94841525480976 58-59 22.292450654100897 26.83085642732395 28.58312189013741 22.293571028437736 60-61 23.298192517975462 26.880038015483663 28.38605883634262 21.435710630198255 62-63 23.212675006863016 26.803061316294823 28.33279670548952 21.651466971352644 64-65 22.69490739032093 26.170208839703268 28.319633884650464 22.815249885325333 66-67 22.21955291392898 26.477956456661307 28.420949258756156 22.881541370653558 68-69 21.843568827922944 26.461207589750618 28.71024595242301 22.984977629903426 70-71 22.047559873288787 26.26621415486699 28.84011392541464 22.84611204642958 72-73 21.67664887718668 27.395293888909944 27.773832493289813 23.154224740613564 74-75 22.217660166066167 26.904030521693457 27.707834793227914 23.17047451901246 76-77 21.793054361042735 26.436562307436297 28.408480546740876 23.361902784780096 78-79 21.59957476663078 26.82436945740894 28.42354219001117 23.15251358594911 80-81 22.00387987608386 26.928099707608794 27.682820294908804 23.385200121398537 82-83 22.36691893659065 27.13191374926013 27.763720060797887 22.73744725335133 84-85 22.14043178801923 27.74539080180588 27.04058546366179 23.073591946513098 86-87 21.712951564906465 27.463900020532982 27.40391603164386 23.41923238291669 88-89 21.25249780746638 26.98310999317439 27.9773222317471 23.787069967612133 90-91 22.564213441984194 26.965047835228102 27.736950575482826 22.733788147304885 92-93 22.142772147271497 27.32605724031247 27.44271073854948 23.08845987386655 94-95 21.650546499063513 26.851533816751573 27.316910122841147 24.18100956134377 96-97 21.475170264433917 27.02580399353483 27.60557976918392 23.89344597284734 98-99 21.53610080105357 26.9461290979595 27.304141314471366 24.213628786515564 100 20.502240426280814 27.781615453470454 27.63871330057833 24.077430819670404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1042.0 1 834.0 2 2000.5 3 2875.0 4 1754.5 5 1195.0 6 1467.0 7 2528.5 8 3814.5 9 4245.0 10 3599.0 11 2882.5 12 2906.5 13 3129.5 14 3290.0 15 3433.0 16 3620.5 17 3754.0 18 3906.5 19 4039.0 20 4168.5 21 4463.5 22 4844.5 23 5410.5 24 6325.5 25 7202.5 26 8359.0 27 9796.5 28 11220.0 29 13127.0 30 15470.5 31 17891.5 32 20594.5 33 23504.5 34 26088.5 35 28550.5 36 32000.0 37 35167.5 38 37135.5 39 38894.5 40 39443.0 41 39633.5 42 40043.0 43 40785.0 44 42563.0 45 44962.0 46 47488.0 47 49930.5 48 53526.0 49 57696.5 50 63319.0 51 65166.0 52 61536.0 53 58643.0 54 56449.0 55 52837.5 56 49093.0 57 47195.5 58 43543.5 59 37428.0 60 29006.5 61 21195.0 62 16820.0 63 13167.5 64 9742.5 65 7246.5 66 5469.0 67 4207.0 68 3219.5 69 2346.0 70 1791.5 71 1383.0 72 1122.0 73 907.0 74 695.0 75 520.5 76 363.0 77 247.0 78 176.0 79 126.5 80 99.0 81 77.0 82 53.5 83 34.5 84 22.0 85 16.0 86 11.0 87 10.0 88 9.5 89 6.0 90 2.5 91 2.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 1.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00276790521638099 2 0.06432084502828206 3 6.590250515192835E-4 4 8.567325669750685E-4 5 0.0012521475978866385 6 5.272200412154267E-4 7 0.0 8 6.590250515192835E-4 9 1.3180501030385667E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0010873913350068177 18-19 0.00118624509273471 20-21 0.003525784025628166 22-23 0.0 24-25 3.295125257596417E-5 26-27 9.885375772789251E-5 28-29 7.908300618231401E-4 30-31 0.0016146113762222443 32-33 0.0029656127318367755 34-35 0.0037234915410839513 36-37 0.001383952608190495 38-39 0.001318050103038567 40-41 0.005074492896698482 42-43 0.0064913967574649414 44-45 0.004876785381242697 46-47 0.006853860535800548 48-49 0.009193399468694003 50-51 0.008435520659446827 52-53 0.0039541503091157 54-55 0.0021747826700136354 56-57 0.002339538932893456 58-59 0.0028338077215329183 60-61 0.007677641850199651 62-63 0.01334525729326549 64-65 0.0037564427936599156 66-67 0.009621765752181537 68-69 0.012455573473714458 70-71 0.005568761685337945 72-73 0.007282226819288081 74-75 0.0030315152369887037 76-77 0.002504295195773277 78-79 0.007150421808984225 80-81 0.005041541644122518 82-83 0.015487088710703159 84-85 0.015585942468431053 86-87 0.02118765540634496 88-89 0.02059453285997761 90-91 0.023889658117574025 92-93 0.03344552136460364 94-95 0.03664179286447216 96-97 0.022472754256807566 98-99 0.01753006637041294 100 0.017859578896172578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1517393.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.72170893908476 #Duplication Level Percentage of deduplicated Percentage of total 1 81.53950951658871 51.958368924517984 2 11.65664663660131 14.8556288836654 3 3.005190316333535 5.744875879318847 4 1.2671772543628648 3.229868007069562 5 0.6488393010560669 2.067257454506694 6 0.40410759171494587 1.5450255803600568 7 0.28434807602825496 1.2683401736642845 8 0.1987989057622615 1.0134244808313093 9 0.15075369087374046 0.8645654530214323 >10 0.765448171005226 8.745730706590955 >50 0.049730000543512176 2.1917872412554447 >100 0.0266617116260384 3.2349771795513385 >500 0.0015617434019772562 0.6025041001656083 >1k 7.808717009886281E-4 0.7616601502211531 >5k 4.462124005649304E-4 1.9159857852600055 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8650 0.5700566695641802 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7568 0.4987501589897937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5652 0.372480959118699 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5084 0.33504833619240365 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2451 0.16152704012737637 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1917 0.12633510237624662 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1599 0.10537810573793341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3180501030385667E-4 0.0 10-11 0.0 0.0 0.0 1.3180501030385667E-4 0.0 12-13 0.0 0.0 0.0 1.3180501030385667E-4 0.0 14-15 0.0 0.0 0.0 1.3180501030385667E-4 3.295125257596417E-5 16-17 0.0 0.0 0.0 1.6475626287982084E-4 6.590250515192834E-5 18-19 0.0 0.0 0.0 1.9770751545578503E-4 6.590250515192834E-5 20-21 0.0 0.0 0.0 2.306587680317492E-4 6.590250515192834E-5 22-23 0.0 0.0 0.0 3.6246377833560586E-4 6.590250515192834E-5 24-25 0.0 0.0 0.0 7.578788092471759E-4 6.590250515192834E-5 26-27 0.0 0.0 0.0 9.88537577278925E-4 6.590250515192834E-5 28-29 0.0 0.0 0.0 0.0023724901854694203 6.590250515192834E-5 30-31 0.0 0.0 0.0 0.0070186167986803685 6.590250515192834E-5 32-33 0.0 3.295125257596417E-5 0.0 0.017332358854957153 6.590250515192834E-5 34-35 0.0 6.590250515192834E-5 0.0 0.03268764255535646 6.590250515192834E-5 36-37 0.0 6.590250515192834E-5 0.0 0.05433661549776492 6.590250515192834E-5 38-39 0.0 6.590250515192834E-5 0.0 0.09361450856831421 1.3180501030385667E-4 40-41 0.0 6.590250515192834E-5 0.0 0.15546400965339896 1.3180501030385667E-4 42-43 0.0 6.590250515192834E-5 0.0 0.21174474905314578 1.3180501030385667E-4 44-45 0.0 6.590250515192834E-5 0.0 0.27217734627746404 1.3180501030385667E-4 46-47 0.0 6.590250515192834E-5 0.0 0.34078185414062145 1.9770751545578503E-4 48-49 0.0 6.590250515192834E-5 0.0 0.4135052685757744 3.9541503091157005E-4 50-51 0.0 6.590250515192834E-5 0.0 0.5035280906133085 3.9541503091157005E-4 52-53 0.0 6.590250515192834E-5 0.0 0.6030738246452962 4.2836628348753424E-4 54-55 0.0 6.590250515192834E-5 0.0 0.7221926027074067 4.613175360634984E-4 56-57 0.0 6.590250515192834E-5 0.0 0.8431566509137711 4.613175360634984E-4 58-59 0.0 6.590250515192834E-5 0.0 0.981288301712213 4.613175360634984E-4 60-61 0.0 6.590250515192834E-5 0.0 1.1295030357988998 4.613175360634984E-4 62-63 0.0 6.590250515192834E-5 0.0 1.3019039892763442 4.613175360634984E-4 64-65 0.0 6.590250515192834E-5 0.0 1.4698235724034578 4.613175360634984E-4 66-67 0.0 6.590250515192834E-5 0.0 1.6548119043649205 4.613175360634984E-4 68-69 0.0 6.590250515192834E-5 0.0 1.8904463115356402 4.613175360634984E-4 70-71 0.0 6.590250515192834E-5 0.0 2.1504316943599977 4.613175360634984E-4 72-73 0.0 6.590250515192834E-5 0.0 2.376543189536264 5.272200412154267E-4 74-75 0.0 6.590250515192834E-5 0.0 2.6179770171603534 5.272200412154267E-4 76-77 0.0 6.590250515192834E-5 0.0 2.8805325976856357 5.931225463673551E-4 78-79 0.0 6.590250515192834E-5 0.0 3.1941955709562384 5.931225463673551E-4 80-81 0.0 6.590250515192834E-5 0.0 3.4885491102173267 6.260737989433193E-4 82-83 0.0 6.590250515192834E-5 0.0 3.81862180727076 6.590250515192835E-4 84-85 0.0 6.590250515192834E-5 0.0 4.166949498251277 6.590250515192835E-4 86-87 0.0 6.590250515192834E-5 0.0 4.537025015931931 6.590250515192835E-4 88 0.0 6.590250515192834E-5 0.0 4.8283470399560295 6.590250515192835E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 345 0.0 70.8847 2 TAACGCA 360 0.0 66.572105 4 TACCTGG 2835 0.0 62.871582 2 GTACCTG 3125 0.0 57.642876 1 ACCTGGG 3195 0.0 55.00794 3 ATAACGC 475 0.0 51.44396 3 GTATAAC 585 0.0 49.042305 1 CCTGGGG 2580 0.0 45.89924 4 TATCACG 190 0.0 39.603596 2 TACCGTA 95 2.122033E-8 39.569668 7 CTGGGGG 1640 0.0 38.39596 5 GTACCCG 350 0.0 34.938335 1 TGGGGGG 3575 0.0 34.700695 6 ATGGGGG 3085 0.0 31.988123 5 GGTATCA 5625 0.0 31.856594 1 TATCTAA 595 0.0 30.825907 2 CATGGGG 4235 0.0 30.29241 4 ATATCTA 615 0.0 29.825405 1 ATCACGC 285 0.0 28.030361 3 ACCGTAT 120 5.855467E-6 27.410236 8 >>END_MODULE