FastQCFastQC Report
Mon 6 Jun 2016
ERR1211554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7911567
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236650.2991189988026392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198420.2507973452035482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120220.15195472654153092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136700.043.2223361
GTATCAA233100.037.8495181
TCAACGC274400.031.5426034
CAACGCA277500.031.1719235
ATCAACG277350.031.1399293
AACGCAG287200.030.2117356
TATCAAC291250.029.8823932
TACCTGG66450.028.8695552
GTACCTG75100.026.9563771
ACGCAGA323250.026.7707637
CGCAGAG330300.025.97318
GTACATG274250.025.561191
TACATGG279200.024.5873872
ACATGGG276700.023.9447443
GCAGAGT366050.022.8221159
CATGGGG160550.022.5065044
ACCTGGG84900.021.8619883
GAGTACT206450.021.30299412-13
AGAGTAC331300.019.65638410-11
ATGGGGG103450.019.4849645
Produced by FastQC (version 0.11.5)