FastQCFastQC Report
Mon 6 Jun 2016
ERR1211554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7911567
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260190.3288729021696966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222790.28160034541829704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144250.18232797624035793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137600.043.059191
GTATCAA232000.036.341771
TCAACGC282100.029.119444
ATCAACG285900.028.716513
CAACGCA286550.028.7158835
AACGCAG296900.027.7457966
TATCAAC298300.027.7123832
TACCTGG66550.026.9912852
GTACCTG71900.026.4874341
ACGCAGA339650.024.266957
TATAACG7050.024.0116312
GTACATG277350.023.7532881
CGCAGAG346850.023.6819218
TACATGG282100.023.0696642
ACATGGG280700.022.0828573
CATGGGG164100.021.5354484
GCAGAGT384950.020.9840249
ACCTGGG83500.020.8804743
TAACGCA8600.020.7648984
AGAGTAC349450.019.7139210-11
Produced by FastQC (version 0.11.5)