##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7911567 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11853543046529 33.0 31.0 34.0 30.0 34.0 2 32.33810116251306 34.0 31.0 34.0 30.0 34.0 3 32.353199814903924 34.0 31.0 34.0 30.0 34.0 4 35.98673701429818 37.0 35.0 37.0 35.0 37.0 5 35.90795982641618 37.0 35.0 37.0 35.0 37.0 6 35.9268875559039 37.0 35.0 37.0 35.0 37.0 7 35.85741257073346 37.0 35.0 37.0 35.0 37.0 8 35.851640642113 37.0 35.0 37.0 35.0 37.0 9 37.505630300546024 39.0 37.0 39.0 35.0 39.0 10-11 37.4684414604591 39.0 37.0 39.0 34.5 39.0 12-13 37.38588967267799 39.0 37.0 39.0 34.0 39.0 14-15 38.618963158625846 40.0 38.0 41.0 34.0 41.0 16-17 38.548164541866356 40.0 38.0 41.0 34.0 41.0 18-19 38.53673008141118 40.0 38.0 41.0 34.0 41.0 20-21 38.478930596176454 40.0 38.0 41.0 34.0 41.0 22-23 38.37384003699899 40.0 38.0 41.0 34.0 41.0 24-25 38.265938719851576 40.0 38.0 41.0 34.0 41.0 26-27 38.09127319531011 40.0 38.0 41.0 33.0 41.0 28-29 38.12904870804987 40.0 38.0 41.0 33.0 41.0 30-31 38.05574919608214 40.0 37.5 41.0 33.0 41.0 32-33 37.88013310131861 40.0 37.0 41.0 33.0 41.0 34-35 37.772003068418684 40.0 37.0 41.0 33.0 41.0 36-37 37.72093568821448 40.0 37.0 41.0 33.0 41.0 38-39 37.631084019133 40.0 37.0 41.0 32.5 41.0 40-41 37.47483848648441 40.0 37.0 41.0 32.0 41.0 42-43 37.334395133100685 40.0 36.0 41.0 31.0 41.0 44-45 37.15834960381426 40.0 36.0 41.0 31.0 41.0 46-47 36.91552065475777 39.0 36.0 41.0 30.5 41.0 48-49 36.7057888784864 39.0 35.5 41.0 30.0 41.0 50-51 36.511480721834246 39.0 35.0 41.0 30.0 41.0 52-53 36.3163686789229 39.0 35.0 40.0 29.0 41.0 54-55 36.12525950927294 39.0 35.0 40.0 28.0 41.0 56-57 35.92672222835249 38.0 35.0 40.0 28.0 41.0 58-59 35.673755401426796 38.0 34.0 40.0 28.0 41.0 60-61 35.365468496948836 38.0 34.0 40.0 27.0 41.0 62-63 35.043307223461554 37.5 34.0 40.0 26.0 41.0 64-65 34.7308727841147 37.0 33.5 40.0 26.0 41.0 66-67 34.39228296998559 36.5 33.0 39.0 26.0 41.0 68-69 33.99666791673508 36.0 32.5 39.0 25.5 40.0 70-71 33.60534645033026 35.5 32.0 39.0 25.0 40.0 72-73 33.1907878679407 35.0 32.0 38.0 24.0 40.0 74-75 32.78020062270849 35.0 31.0 37.0 23.5 39.0 76-77 31.227633729196757 33.5 29.5 35.5 21.5 38.5 78-79 32.066474947377685 34.0 31.0 36.0 23.0 39.0 80-81 32.03296862682197 34.5 31.0 36.0 23.0 37.5 82-83 31.744773001353586 34.0 31.0 35.5 22.5 37.0 84-85 31.442169231455665 34.0 31.0 35.0 21.5 37.0 86-87 31.147242575333053 34.0 30.0 35.0 20.0 36.0 88-89 30.867920539635193 34.0 30.0 35.0 20.0 36.0 90-91 30.585078720814725 34.0 29.5 35.0 20.0 35.5 92-93 30.375341572661902 34.0 29.0 35.0 19.0 35.0 94-95 30.15527745641287 34.0 29.0 35.0 19.0 35.0 96-97 29.952253832900613 34.0 29.0 35.0 18.0 35.0 98-99 29.797973208088866 34.0 29.0 35.0 18.0 35.0 100 27.89150998784438 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32467906720371786 1101 2 0.20946217990839955 1101 3 0.2643113218660531 1101 4 0.16284229891508772 1101 5 0.1800612896398519 1101 6 0.16041958783233667 1101 7 0.2024100260789652 1101 8 0.18336826093403147 1101 9 0.2152149673321091 1101 10-11 0.21517839898611868 1101 12-13 0.20775370967822226 1101 14-15 0.25278616345939753 1101 16-17 0.24440449351158833 1101 18-19 0.2826758617637424 1101 20-21 0.2290215963051523 1101 22-23 0.2589154865354928 1101 24-25 0.2099683941977517 1101 26-27 0.24973617174094187 1101 28-29 0.3206890911797302 1101 30-31 0.32701410243352314 1101 32-33 0.30391265071408924 1101 34-35 0.3309085974982935 1101 36-37 0.3378844265938383 1101 38-39 0.35472131554738695 1101 40-41 0.2937849351626056 1101 42-43 0.3145409647054649 1101 44-45 0.3280875429589756 1101 46-47 0.36479077286557526 1101 48-49 0.348827312161049 1101 50-51 0.3032436415642863 1101 52-53 0.2923228600371601 1101 54-55 0.25972076536648814 1101 56-57 0.29301693681831154 1101 58-59 0.24650503701312942 1101 60-61 0.17673547879125096 1101 62-63 0.07665427556266025 1101 64-65 0.1781647852204813 1101 66-67 0.1139498735655522 1101 68-69 0.09233426739145756 1101 70-71 0.09116942967570907 1101 72-73 0.08733157859480656 1101 74-75 0.07853101928657935 1101 76-77 0.15274945763633951 1101 78-79 0.22746217672229108 1101 80-81 0.24346091060163388 1101 82-83 0.1847485602169492 1101 84-85 0.1944941348928495 1101 86-87 0.1441086107277556 1101 88-89 0.09367835118692369 1101 90-91 -0.06481941876291941 1101 92-93 -0.04765788566151841 1101 94-95 0.066448799884963 1101 96-97 -0.0035084639567699583 1101 98-99 -0.14147420921455733 1101 100 -0.04476792699364651 1102 1 0.34983178830061945 1102 2 0.2350372011307016 1102 3 0.3376783918623829 1102 4 0.23004322752468198 1102 5 0.21699214327971816 1102 6 0.19103275158732202 1102 7 0.251911809283591 1102 8 0.21023413456314444 1102 9 0.26187297860589354 1102 10-11 0.23318814222148632 1102 12-13 0.2817708329747859 1102 14-15 0.3515016155511219 1102 16-17 0.30913641338394626 1102 18-19 0.34232039312080786 1102 20-21 0.3210307013682936 1102 22-23 0.30574589672730923 1102 24-25 0.3190392256819905 1102 26-27 0.34187506338857787 1102 28-29 0.38682048946243697 1102 30-31 0.3798160944018747 1102 32-33 0.4072660679263649 1102 34-35 0.34035308445801604 1102 36-37 0.4484425690404308 1102 38-39 0.3989645519629832 1102 40-41 0.4272160705110153 1102 42-43 0.3152677670467128 1102 44-45 0.41980309639108526 1102 46-47 0.4430244979703204 1102 48-49 0.4845286809778173 1102 50-51 0.44988081507782596 1102 52-53 0.4295244906235496 1102 54-55 0.46437521966245043 1102 56-57 0.4417897022454511 1102 58-59 0.4912969230475426 1102 60-61 0.4179367566801986 1102 62-63 0.46859665168638287 1102 64-65 0.4219740429968084 1102 66-67 0.40597617041387224 1102 68-69 0.4404488457024698 1102 70-71 0.410879197401151 1102 72-73 0.38224257597597244 1102 74-75 0.4213071811673572 1102 76-77 0.4908335534437036 1102 78-79 0.3860428745884974 1102 80-81 0.3780593972814472 1102 82-83 0.36088455837826316 1102 84-85 0.29571364392306165 1102 86-87 0.25645481910041923 1102 88-89 0.20916692165643624 1102 90-91 0.2460419915850558 1102 92-93 0.1511635212506306 1102 94-95 0.19019929543057046 1102 96-97 0.3041493116578948 1102 98-99 0.30659990890098143 1102 100 0.43036537738710123 1103 1 0.29168435788551506 1103 2 0.22913494401934997 1103 3 0.2917559600892474 1103 4 0.16798908818181957 1103 5 0.16540286755891742 1103 6 0.16413084440592485 1103 7 0.18324658360403845 1103 8 0.20980171210042187 1103 9 0.25022921740213633 1103 10-11 0.217080752888414 1103 12-13 0.28484873218233275 1103 14-15 0.3405940336934137 1103 16-17 0.3558281857349286 1103 18-19 0.3546740889242983 1103 20-21 0.3420046751702799 1103 22-23 0.36170525432160616 1103 24-25 0.33708708397173126 1103 26-27 0.3964061825201455 1103 28-29 0.4365000887989581 1103 30-31 0.45465540454718933 1103 32-33 0.46450380679777226 1103 34-35 0.4796925695683072 1103 36-37 0.49522125540087103 1103 38-39 0.5005186136138136 1103 40-41 0.49383663780179177 1103 42-43 0.5310046980473473 1103 44-45 0.5305885501693837 1103 46-47 0.6287075624976595 1103 48-49 0.5769667515878751 1103 50-51 0.5656745939044114 1103 52-53 0.5339772860838039 1103 54-55 0.5790791940834552 1103 56-57 0.5598292544361811 1103 58-59 0.5852185640042507 1103 60-61 0.5238206329944859 1103 62-63 0.6480748075934386 1103 64-65 0.6483611931142335 1103 66-67 0.6411739755824826 1103 68-69 0.6523477904804977 1103 70-71 0.6443938016076345 1103 72-73 0.621556669057334 1103 74-75 0.5855715008188938 1103 76-77 0.603014495020556 1103 78-79 0.6206747280676481 1103 80-81 0.6066414735409111 1103 82-83 0.5689961746630168 1103 84-85 0.4812975721583648 1103 86-87 0.4863754405861158 1103 88-89 0.4800749835624565 1103 90-91 0.5372711300120372 1103 92-93 0.4779956950988655 1103 94-95 0.5520672581434525 1103 96-97 0.5422716848146045 1103 98-99 0.5692492449815383 1103 100 0.7245209787297213 1104 1 0.27337588405497115 1104 2 0.23840648534591224 1104 3 0.29065157668843966 1104 4 0.08636253923719295 1104 5 0.15571058022040063 1104 6 0.21372424604393814 1104 7 0.21291976269314716 1104 8 0.179980275836094 1104 9 0.21624588664766975 1104 10-11 0.23106291359399478 1104 12-13 0.2827663319353704 1104 14-15 0.3359787133944465 1104 16-17 0.2703107398981217 1104 18-19 0.2855621114218607 1104 20-21 0.2072314913143103 1104 22-23 0.3152688434198154 1104 24-25 0.31450092159698073 1104 26-27 0.3073064561178498 1104 28-29 0.33887769059639794 1104 30-31 0.27943946807345554 1104 32-33 0.3262868109072201 1104 34-35 0.4176815142632293 1104 36-37 0.29858587245967527 1104 38-39 0.45028476315626165 1104 40-41 0.4882874387754583 1104 42-43 0.5163046142065824 1104 44-45 0.4321379153987408 1104 46-47 0.4431092328890216 1104 48-49 0.41567221739860827 1104 50-51 0.4018707190797599 1104 52-53 0.47870376230186196 1104 54-55 0.49560586061927125 1104 56-57 0.5352576605721282 1104 58-59 0.5590353297597019 1104 60-61 0.5055052637777848 1104 62-63 0.5400023560759024 1104 64-65 0.6335874076644927 1104 66-67 0.5862887729282349 1104 68-69 0.5838199516015763 1104 70-71 0.4548864189560149 1104 72-73 0.5565221717335405 1104 74-75 0.5947712714995106 1104 76-77 0.457824498454233 1104 78-79 0.5987873257724701 1104 80-81 0.6672998414425209 1104 82-83 0.5226565413344169 1104 84-85 0.6047647316390901 1104 86-87 0.6723425968804335 1104 88-89 0.6353489598142907 1104 90-91 0.4665614558489857 1104 92-93 0.5121772331548939 1104 94-95 0.49690342325850523 1104 96-97 0.4651265314553541 1104 98-99 0.616208226738209 1104 100 0.6308763707560026 1105 1 0.23866854224711886 1105 2 0.18551263550880748 1105 3 0.22061551613195718 1105 4 0.15127590874227792 1105 5 0.11216712035518839 1105 6 0.1448553812540112 1105 7 0.1857669539960014 1105 8 0.17581616345051998 1105 9 0.16674960852402876 1105 10-11 0.20939824207969338 1105 12-13 0.2232267903323688 1105 14-15 0.25948301327573375 1105 16-17 0.29941492946509385 1105 18-19 0.2884035905898159 1105 20-21 0.19328646483667455 1105 22-23 0.28429698748599463 1105 24-25 0.3316520211146994 1105 26-27 0.30102736902340865 1105 28-29 0.35688094289784544 1105 30-31 0.3938852035575948 1105 32-33 0.4005187504836343 1105 34-35 0.4923959657780941 1105 36-37 0.4965212809245898 1105 38-39 0.46199259561246464 1105 40-41 0.48430310649765573 1105 42-43 0.5624940396260101 1105 44-45 0.5863955532568426 1105 46-47 0.6028757418011566 1105 48-49 0.5973510390808627 1105 50-51 0.6517690239026805 1105 52-53 0.611861337126065 1105 54-55 0.5139324521153341 1105 56-57 0.5406657652785753 1105 58-59 0.6455468612610389 1105 60-61 0.6211227626057578 1105 62-63 0.6100798643999141 1105 64-65 0.6027835666649182 1105 66-67 0.5477521967215679 1105 68-69 0.6675417254222111 1105 70-71 0.687480144343283 1105 72-73 0.6766128601147088 1105 74-75 0.6493322204582981 1105 76-77 0.5868568452241654 1105 78-79 0.6844149445139962 1105 80-81 0.6617837261843036 1105 82-83 0.6758789637709945 1105 84-85 0.7046461743782793 1105 86-87 0.7016846533541994 1105 88-89 0.6972019193813104 1105 90-91 0.7380741555448296 1105 92-93 0.6717254105417503 1105 94-95 0.7538100295532573 1105 96-97 0.7262995678615418 1105 98-99 0.7441657746363468 1105 100 0.9049797190498161 1106 1 0.26061375172736234 1106 2 0.19709944741484264 1106 3 0.23831036028420982 1106 4 0.1739490851062655 1106 5 0.14726386547249604 1106 6 0.14457734732965832 1106 7 0.14188518104636927 1106 8 0.1861466487061776 1106 9 0.187029609926455 1106 10-11 0.16577070950483375 1106 12-13 0.20815509141505828 1106 14-15 0.28987707734675894 1106 16-17 0.2974841118486111 1106 18-19 0.29360661696593837 1106 20-21 0.33576836693183765 1106 22-23 0.3063228363353403 1106 24-25 0.29030625250305775 1106 26-27 0.2998471885396299 1106 28-29 0.3738175591283124 1106 30-31 0.3882098803278424 1106 32-33 0.3401038985431626 1106 34-35 0.39165012820260614 1106 36-37 0.3791271069062603 1106 38-39 0.40349991527761375 1106 40-41 0.45437657376245966 1106 42-43 0.47184293033259905 1106 44-45 0.5098838374015244 1106 46-47 0.5022477486730352 1106 48-49 0.564472404224702 1106 50-51 0.5284657780063924 1106 52-53 0.6103505999038745 1106 54-55 0.4755540012422088 1106 56-57 0.5264440645401578 1106 58-59 0.6054200780638439 1106 60-61 0.6562891183223911 1106 62-63 0.7074833039464323 1106 64-65 0.7265198279323855 1106 66-67 0.7267764977988094 1106 68-69 0.7122362233936386 1106 70-71 0.7297440239989825 1106 72-73 0.7386420652501613 1106 74-75 0.6837234306649478 1106 76-77 0.5941057764717002 1106 78-79 0.7413714261458253 1106 80-81 0.7631808738857373 1106 82-83 0.7498396979466726 1106 84-85 0.7499475270024298 1106 86-87 0.6472908289206387 1106 88-89 0.7137567495569748 1106 90-91 0.7202557483238472 1106 92-93 0.7705671854792833 1106 94-95 0.7909404253533374 1106 96-97 0.8285910330574353 1106 98-99 0.8574362439819829 1106 100 0.9692311532153788 1107 1 0.22202853836889602 1107 2 0.19379870298990198 1107 3 0.26397405791981754 1107 4 0.14478654961875748 1107 5 0.1292028242528076 1107 6 0.15153804559374606 1107 7 0.18405083997622285 1107 8 0.19443345548076962 1107 9 0.19684644292387077 1107 10-11 0.19020467023560172 1107 12-13 0.18159587604110783 1107 14-15 0.20799625983380565 1107 16-17 0.2143106416039373 1107 18-19 0.20271332735537584 1107 20-21 0.27255069307014423 1107 22-23 0.27516043765082543 1107 24-25 0.2885698027707022 1107 26-27 0.3602896581449855 1107 28-29 0.32600119207371847 1107 30-31 0.27336970278194883 1107 32-33 0.33531278044291213 1107 34-35 0.336977662985781 1107 36-37 0.4161647873236589 1107 38-39 0.43754675743135607 1107 40-41 0.4115021805717305 1107 42-43 0.4043403771284275 1107 44-45 0.40745612626440675 1107 46-47 0.4158826377678011 1107 48-49 0.4639486248865552 1107 50-51 0.40094884017183574 1107 52-53 0.42500003210377457 1107 54-55 0.4553791507773468 1107 56-57 0.4549970140666346 1107 58-59 0.5568830911427511 1107 60-61 0.5901509039670216 1107 62-63 0.5366329718267124 1107 64-65 0.5851106827207602 1107 66-67 0.5732051611388655 1107 68-69 0.5623770221063253 1107 70-71 0.6618747778511391 1107 72-73 0.6351504061686128 1107 74-75 0.6362262479936831 1107 76-77 0.588778383909446 1107 78-79 0.6701818632652063 1107 80-81 0.6199440543789834 1107 82-83 0.668304307963357 1107 84-85 0.5599251035037476 1107 86-87 0.6051586934938129 1107 88-89 0.5635022756931178 1107 90-91 0.517452912574111 1107 92-93 0.5368419996439435 1107 94-95 0.7140818688008785 1107 96-97 0.7148154806984621 1107 98-99 0.8187888597633588 1107 100 0.9136457312662216 1108 1 0.22578692777372567 1108 2 0.14897833432512897 1108 3 0.22357102536849993 1108 4 0.13029145427589128 1108 5 0.1058764145769544 1108 6 0.13488576990106083 1108 7 0.13664249449050914 1108 8 0.1327216811056573 1108 9 0.15977372160135417 1108 10-11 0.15501889875147867 1108 12-13 0.22081066719076858 1108 14-15 0.2060561833164627 1108 16-17 0.25555790465388384 1108 18-19 0.28120269882159477 1108 20-21 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-0.32058199336155724 2316 64-65 -0.3732973209476853 2316 66-67 -0.09892263013396985 2316 68-69 -0.1153166869953921 2316 70-71 -0.3373562106477692 2316 72-73 -0.45663516844526697 2316 74-75 -0.5129858061187278 2316 76-77 -0.4224019237777732 2316 78-79 -0.3550456247820293 2316 80-81 -0.26744092782957196 2316 82-83 -0.12693138727977527 2316 84-85 -0.16239960538867138 2316 86-87 -0.1770329996121447 2316 88-89 -0.2002101102874505 2316 90-91 -0.13493970535613542 2316 92-93 0.025204443735862725 2316 94-95 0.06137232366681289 2316 96-97 0.039340158745041265 2316 98-99 -0.08875446211294857 2316 100 -0.3274642644373955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 15.0 11 34.0 12 89.0 13 248.0 14 710.0 15 1863.0 16 4395.0 17 8961.0 18 15928.0 19 24089.0 20 33814.0 21 47074.0 22 60999.0 23 70772.0 24 76689.0 25 85489.0 26 99628.0 27 119397.0 28 143949.0 29 174162.0 30 210680.0 31 255922.0 32 312739.0 33 394410.0 34 520513.0 35 708290.0 36 1039063.0 37 1622913.0 38 1632510.0 39 246195.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92404005358916 20.58537527519178 14.043935378011781 23.446649293207276 2 15.027103340521345 22.14625672619068 39.67904514685334 23.14759478643463 3 17.090973806943474 27.75751609748156 31.110982184636367 24.040527910938604 4 12.066725003561265 18.361775624068052 39.07753721568189 30.493962156688788 5 12.509761164003482 38.52377830927159 35.454645884309 13.51181464241592 6 29.39570544470294 38.75253333562536 18.761952528380814 13.089808691290886 7 26.28723235232666 33.292216320736465 23.301110892443937 17.119440434492937 8 25.57776036851681 36.02319055127107 20.887687767853823 17.511361312358293 9 26.427119485573396 15.697562277809476 20.66162375438693 37.21369448223019 10-11 23.974258196890705 26.78356259891372 29.609090082912775 19.6330891212828 12-13 24.940065602680228 24.542224062565605 29.32264872432983 21.195061610424332 14-15 22.416899205934804 25.987165374444782 26.85027504665005 24.74566037297036 16-17 21.889092804259214 28.935304546611953 27.31438064292812 21.861222006200716 18-19 21.718297206838642 28.018411464803027 29.2408054696695 21.022485858688828 20-21 22.5556442609888 27.221542325131757 29.108646487669326 21.114166926210114 22-23 22.266113653591002 27.130826295220658 28.894547692005894 21.708512359182446 24-25 21.993222705825126 27.23396238506303 29.043939412994735 21.72887549611711 26-27 21.998729073718465 27.324712817111436 28.97236667518796 21.704191433982142 28-29 22.04707385454788 27.399148806867185 29.033292493960992 21.520484844623947 30-31 22.392063637021486 27.213086884075672 29.037427798315917 21.35742168058692 32-33 22.19298291073968 27.350223596676315 28.912505752815925 21.544287739768077 34-35 22.31266260513934 27.453862248304706 28.87617629501869 21.357298851537262 36-37 22.36251016167483 27.385276800451596 28.756830355029628 21.495382682843946 38-39 22.24405977819228 27.488884144708294 28.747105301975733 21.519950775123693 40-41 22.294781891322884 27.29780074574837 28.8932967527264 21.514120610202344 42-43 22.137826736681955 27.435768826173675 28.614439022762383 21.81196541438199 44-45 22.070493613552962 27.581483955258975 28.63341991180019 21.714602519387878 46-47 22.085300424573166 27.428579229640132 28.448550904537527 22.03756944124918 48-49 22.102711504485633 27.524355265001628 28.45326741387938 21.919665816633362 50-51 22.235024505065184 27.67512179848211 28.26475646213134 21.825097234321365 52-53 22.230073902279514 27.755042244861688 28.038233615943252 21.976650236915546 54-55 22.127724845300563 27.67049960060745 28.248508052852156 21.953267501239825 56-57 22.233975372610047 27.69274387165585 28.05413586599715 22.019144889736953 58-59 22.17129293173286 27.605086929188776 28.229883462196554 21.99373667688181 60-61 22.340012423274146 27.745887413382803 28.174211012117624 21.739889151225427 62-63 22.186639924004385 27.810455692411683 28.177748022506666 21.825156361077266 64-65 22.113372266091552 27.515997162015037 28.16533221627387 22.205298355619536 66-67 22.060186715568904 27.533322065963667 28.102914148837232 22.303577069630194 68-69 21.924503856507783 27.6753331720727 28.189504713902803 22.210658257516712 70-71 22.030949650914845 27.495987603251987 28.162904382408176 22.310158363424993 72-73 21.970285384616307 27.765729436855004 27.89474672180876 22.369238456719927 74-75 21.98401290707574 27.765545305053095 27.810790601997553 22.439651185873608 76-77 21.8711975099558 27.614790682570998 28.013635607242787 22.500376200230416 78-79 21.773219093467453 27.540429053021022 28.09198052776674 22.594371325744785 80-81 21.815643033631154 27.748702548714228 27.7968438471224 22.63881057053222 82-83 21.93090815196831 27.712103288542387 27.81379390772815 22.543194651761144 84-85 21.860140189007122 27.750614461520335 27.77632184762467 22.612923501847874 86-87 21.627956415164817 27.741996780057875 27.928083112914987 22.70196369186232 88-89 21.593724466310732 27.585278334359266 27.936041612699437 22.884955586630564 90-91 21.801439648236855 27.5673338124533 27.875603195872177 22.75562334343767 92-93 21.66220510666809 27.5752920255023 27.790272562193103 22.972230305636508 94-95 21.496006952972916 27.466247171814285 27.85781026241252 23.179935612800286 96-97 21.458301516005086 27.368422915758504 27.983639403388434 23.18963616484798 98-99 21.32944211673081 27.361769681822246 27.860251216084205 23.448536985362743 100 20.513928158527342 27.8236876987581 28.08133445342668 23.581049689287877 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2085.0 1 1597.5 2 2026.0 3 2807.0 4 2073.0 5 1688.5 6 2490.0 7 4596.0 8 7469.5 9 9084.0 10 7938.5 11 6310.5 12 6370.0 13 7012.0 14 7679.0 15 8236.0 16 8793.5 17 9451.5 18 10219.0 19 11083.5 20 12115.5 21 13617.0 22 15988.0 23 19689.5 24 24567.5 25 30768.5 26 38966.0 27 49095.5 28 61973.5 29 77689.5 30 96050.5 31 115143.5 32 135329.5 33 156625.5 34 178321.0 35 197493.5 36 218714.0 37 239326.0 38 252551.5 39 263077.0 40 269214.0 41 273824.0 42 278463.5 43 284180.5 44 290109.5 45 296437.5 46 302047.5 47 303896.0 48 308411.5 49 311370.5 50 308233.5 51 297107.5 52 279118.0 53 260724.5 54 241343.5 55 218270.0 56 192641.5 57 168403.0 58 145434.0 59 122023.0 60 99684.5 61 80906.5 62 65233.0 63 50407.5 64 36855.0 65 27198.5 66 20441.0 67 15369.5 68 11690.0 69 9043.0 70 7014.0 71 5360.0 72 4050.5 73 3130.0 74 2468.0 75 1874.5 76 1358.0 77 932.0 78 624.5 79 439.0 80 318.5 81 238.0 82 159.5 83 126.0 84 94.5 85 57.0 86 41.5 87 30.0 88 21.0 89 13.0 90 9.0 91 7.0 92 4.5 93 6.5 94 7.5 95 6.5 96 5.5 97 5.0 98 6.0 99 4.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00336216580103537 2 0.06757194876817703 3 0.0010238174055784398 4 6.319860528261974E-4 5 0.0014282884793872061 6 7.710229844479608E-4 7 0.0 8 8.721407529001524E-4 9 1.2639721056523946E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.647031239196988E-4 18-19 0.0010554167082197495 20-21 0.0030208933325092235 22-23 0.0 24-25 3.159930264130987E-5 26-27 1.8327595531959724E-4 28-29 0.0010617365687480116 30-31 0.001700042482102471 32-33 0.0028312975166613643 34-35 0.00429118529868988 36-37 0.0012702919661806567 38-39 0.0015167665267828737 40-41 0.005732113499133611 42-43 0.006736971323127264 44-45 0.005548837543814013 46-47 0.0071161629548229825 48-49 0.008854124600095025 50-51 0.008234778268325353 52-53 0.0039056738064659 54-55 0.002243550487533001 56-57 0.002692260585039601 58-59 0.0034316842668462516 60-61 0.007697590123423084 62-63 0.014036410233269844 64-65 0.004044710738087663 66-67 0.01033929182423659 68-69 0.01235532733275216 70-71 0.005814271686001016 72-73 0.007729189426064395 74-75 0.003128330961489677 76-77 0.0027996982140200545 78-79 0.007710229844479608 80-81 0.006067066107131495 82-83 0.01642531751295287 84-85 0.017556572547511765 86-87 0.022719898599101795 88-89 0.021158893048621088 90-91 0.024350422615393386 92-93 0.034272603644764685 94-95 0.03801396107749577 96-97 0.02314332925449535 98-99 0.018258077066148844 100 0.01911125823746421 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7911567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.70783165851278 #Duplication Level Percentage of deduplicated Percentage of total 1 75.40081278167503 45.77419849264967 2 14.891172030871621 18.080215296962166 3 4.668408082729214 8.502267959986966 4 1.923285330125618 4.670339282102128 5 0.992967389124394 3.014044855067833 6 0.5519259118848427 2.0103735208005715 7 0.36015090360782925 1.5304786299519753 8 0.22703142007710272 1.102606818498708 9 0.17495313140440616 0.9558922724485518 >10 0.746052885186573 7.681138070761224 >50 0.03760279361467706 1.575871016285823 >100 0.023494232994862634 2.6921440723454992 >500 0.001428740397445466 0.6228702354626977 >1k 6.250705179664817E-4 0.6541645507067712 >5k 0.0 0.0 >10k+ 8.929578826682481E-5 1.1333949259695166 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26019 0.3288729021696966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22279 0.28160034541829704 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14425 0.18232797624035793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2639721056523948E-5 2 0.0 0.0 0.0 0.0 1.2639721056523948E-5 3 0.0 0.0 0.0 0.0 1.2639721056523948E-5 4 0.0 0.0 0.0 0.0 1.2639721056523948E-5 5 0.0 0.0 0.0 0.0 1.2639721056523948E-5 6 1.2639721056523948E-5 0.0 0.0 0.0 1.2639721056523948E-5 7 2.5279442113047896E-5 0.0 0.0 0.0 1.2639721056523948E-5 8 2.5279442113047896E-5 0.0 0.0 1.2639721056523948E-5 1.2639721056523948E-5 9 2.5279442113047896E-5 0.0 0.0 2.5279442113047896E-5 2.5279442113047896E-5 10-11 2.5279442113047896E-5 0.0 0.0 2.5279442113047896E-5 2.5279442113047896E-5 12-13 2.5279442113047896E-5 0.0 0.0 5.0558884226095785E-5 2.5279442113047896E-5 14-15 2.5279442113047896E-5 0.0 0.0 6.951846581088171E-5 3.791916316957184E-5 16-17 2.5279442113047896E-5 0.0 0.0 7.583832633914368E-5 6.319860528261973E-5 18-19 2.5279442113047896E-5 0.0 0.0 8.215818686740566E-5 7.583832633914368E-5 20-21 2.5279442113047896E-5 0.0 0.0 1.1375748950871552E-4 1.0111776845219158E-4 22-23 2.5279442113047896E-5 0.0 0.0 2.211951184891691E-4 1.0111776845219158E-4 24-25 2.5279442113047896E-5 6.319860528261974E-6 0.0 4.107909343370283E-4 1.0111776845219158E-4 26-27 2.5279442113047896E-5 1.2639721056523948E-5 0.0 6.256661922979354E-4 1.1375748950871553E-4 28-29 2.5279442113047896E-5 1.2639721056523948E-5 0.0 0.0016431637373481131 1.1375748950871553E-4 30-31 2.5279442113047896E-5 1.2639721056523948E-5 0.0 0.004651417348800813 1.1375748950871553E-4 32-33 2.5279442113047896E-5 1.2639721056523948E-5 0.0 0.011647502953586818 1.3903693162176342E-4 34-35 6.319860528261973E-5 3.159930264130987E-5 0.0 0.022865255391251824 1.5167665267828737E-4 36-37 6.319860528261973E-5 5.0558884226095785E-5 0.0 0.04167316032335946 1.5167665267828737E-4 38-39 6.319860528261973E-5 6.951846581088171E-5 0.0 0.07015045186370791 1.5799651320654934E-4 40-41 6.319860528261973E-5 7.583832633914368E-5 0.0 0.10398698513202251 1.6431637373481132E-4 42-43 6.319860528261973E-5 8.847804739566763E-5 0.0 0.13785511770297842 1.6431637373481132E-4 44-45 6.319860528261973E-5 8.847804739566763E-5 0.0 0.18111456301893164 1.7695609479133527E-4 46-47 6.319860528261973E-5 8.847804739566763E-5 0.0 0.23402443536154088 1.8959581584785922E-4 48-49 6.319860528261973E-5 9.479790792392961E-5 0.0 0.2921102734767967 2.0855539743264514E-4 50-51 7.583832633914368E-5 1.1375748950871553E-4 0.0 0.3558650264859035 2.1487525796090712E-4 52-53 7.583832633914368E-5 1.3903693162176342E-4 0.0 0.4242016783780002 2.2751497901743107E-4 54-55 7.583832633914368E-5 1.3903693162176342E-4 0.0 0.5008110277015918 2.40154700073955E-4 56-57 7.583832633914368E-5 1.3903693162176342E-4 0.0 0.5901801754317444 2.654341421870029E-4 58-59 8.215818686740566E-5 1.3903693162176342E-4 0.0 0.6941734804242952 2.654341421870029E-4 60-61 8.847804739566763E-5 1.3903693162176342E-4 0.0 0.8068186744800366 2.654341421870029E-4 62-63 8.847804739566763E-5 1.3903693162176342E-4 0.0 0.928968938770284 2.654341421870029E-4 64-65 8.847804739566763E-5 1.3903693162176342E-4 0.0 1.051757508973886 2.654341421870029E-4 66-67 8.847804739566763E-5 1.3903693162176342E-4 0.0 1.1930253513621258 2.654341421870029E-4 68-69 8.847804739566763E-5 1.3903693162176342E-4 0.0 1.3586562560868156 2.717540027152649E-4 70-71 1.0111776845219158E-4 1.3903693162176342E-4 0.0 1.5404091250191017 2.7807386324352684E-4 72-73 1.0111776845219158E-4 1.3903693162176342E-4 0.0 1.7246393792784667 3.349526079978846E-4 74-75 1.0111776845219158E-4 1.3903693162176342E-4 0.0 1.912383475991545 3.412724685261466E-4 76-77 1.0111776845219158E-4 1.3903693162176342E-4 0.0 2.119403147315823 3.475923290544086E-4 78-79 1.0111776845219158E-4 1.3903693162176342E-4 0.0 2.3589385010580077 3.5391218958267053E-4 80-81 1.0111776845219158E-4 1.3903693162176342E-4 0.0 2.621844699033706 3.7919163169571843E-4 82-83 1.0111776845219158E-4 1.453567921500254E-4 0.0 2.8954251414416383 3.918313527522424E-4 84-85 1.0111776845219158E-4 1.5167665267828737E-4 0.0 3.1756414374042463 4.0447107380876633E-4 86-87 1.0111776845219158E-4 1.5167665267828737E-4 0.0 3.4683710572128126 4.0447107380876633E-4 88 1.0111776845219158E-4 1.5167665267828737E-4 0.0 3.7061558095886693 4.0447107380876633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13760 0.0 43.05919 1 GTATCAA 23200 0.0 36.34177 1 TCAACGC 28210 0.0 29.11944 4 ATCAACG 28590 0.0 28.71651 3 CAACGCA 28655 0.0 28.715883 5 AACGCAG 29690 0.0 27.745796 6 TATCAAC 29830 0.0 27.712383 2 TACCTGG 6655 0.0 26.991285 2 GTACCTG 7190 0.0 26.487434 1 ACGCAGA 33965 0.0 24.26695 7 TATAACG 705 0.0 24.011631 2 GTACATG 27735 0.0 23.753288 1 CGCAGAG 34685 0.0 23.681921 8 TACATGG 28210 0.0 23.069664 2 ACATGGG 28070 0.0 22.082857 3 CATGGGG 16410 0.0 21.535448 4 GCAGAGT 38495 0.0 20.984024 9 ACCTGGG 8350 0.0 20.880474 3 TAACGCA 860 0.0 20.764898 4 AGAGTAC 34945 0.0 19.71392 10-11 >>END_MODULE