FastQCFastQC Report
Mon 6 Jun 2016
ERR1211553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6811861
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238840.350623713549058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192090.2819934229427171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130170.19109315354497106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110950.047.996541
GTATCAA192650.038.297871
TCAACGC235400.030.5989764
ATCAACG237100.030.3421753
TACCTGG55950.030.256242
CAACGCA239350.030.1314895
TATCAAC246850.029.335952
AACGCAG249450.029.0564146
GTACATG249150.029.0271871
TACATGG255300.027.6098082
ACATGGG255150.026.556623
GTACCTG66700.025.6950931
CATGGGG152900.025.5059684
ACGCAGA286300.025.235427
CGCAGAG289950.024.7732058
ACCTGGG68800.024.382763
ATGGGGG98900.022.660415
GCAGAGT325300.021.4620369
GAGTACT196150.020.2893412-13
AGAGTAC301350.019.3401610-11
Produced by FastQC (version 0.11.5)