FastQCFastQC Report
Mon 6 Jun 2016
ERR1211553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6811861
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266630.3914202007351588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219380.32205589632554155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161350.2368662543172857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123500.048.3639871
GTATCAA215500.037.9086151
TACCTGG58400.030.0384942
TCAACGC268350.030.0324444
ATCAACG270550.029.7361243
CAACGCA273300.029.5224535
TATCAAC280700.028.8517992
AACGCAG283350.028.6739276
GTACCTG65900.027.4764521
GTACATG261100.026.2444821
ACGCAGA323650.025.0309337
TACATGG268450.024.912562
CGCAGAG328700.024.574898
TATAACG7250.024.0019152
CATGGGG156950.023.982774
ACATGGG266150.023.9420533
ACCTGGG72300.023.5287273
ATGGGGG95250.023.0395345
GCAGAGT364900.021.7504379
TAACGCA8050.021.015264
Produced by FastQC (version 0.11.5)