##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211553_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6811861
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08973876595544	33.0	31.0	34.0	30.0	34.0
2	32.315814136548	34.0	31.0	34.0	30.0	34.0
3	32.33087932945197	34.0	31.0	34.0	30.0	34.0
4	35.97655970960065	37.0	35.0	37.0	35.0	37.0
5	35.897611827369936	37.0	35.0	37.0	35.0	37.0
6	35.91397108660908	37.0	35.0	37.0	35.0	37.0
7	35.8393519773818	37.0	35.0	37.0	35.0	37.0
8	35.83361580631196	37.0	35.0	37.0	35.0	37.0
9	37.48308619333248	39.0	37.0	39.0	35.0	39.0
10-11	37.447676266441725	39.0	37.0	39.0	34.0	39.0
12-13	37.35488385626189	39.0	37.0	39.0	34.0	39.0
14-15	38.57918863875819	40.0	38.0	41.0	34.0	41.0
16-17	38.511140934907516	40.0	38.0	41.0	34.0	41.0
18-19	38.497127436980875	40.0	38.0	41.0	34.0	41.0
20-21	38.43682122991059	40.0	38.0	41.0	34.0	41.0
22-23	38.32946187539646	40.0	38.0	41.0	34.0	41.0
24-25	38.21352336754963	40.0	38.0	41.0	33.5	41.0
26-27	38.036707369689424	40.0	37.0	41.0	33.0	41.0
28-29	38.06828163404978	40.0	37.5	41.0	33.0	41.0
30-31	37.984397288787896	40.0	37.0	41.0	33.0	41.0
32-33	37.79528156842895	40.0	37.0	41.0	33.0	41.0
34-35	37.680392480116666	40.0	37.0	41.0	33.0	41.0
36-37	37.6162195617321	40.0	37.0	41.0	33.0	41.0
38-39	37.515789884731944	40.0	37.0	41.0	32.0	41.0
40-41	37.34702609169506	40.0	36.0	41.0	31.0	41.0
42-43	37.192360942185985	40.0	36.0	41.0	31.0	41.0
44-45	37.00509192715471	39.0	36.0	41.0	31.0	41.0
46-47	36.73793651984384	39.0	35.5	41.0	30.0	41.0
48-49	36.50721520888345	39.0	35.0	41.0	30.0	41.0
50-51	36.305391434147	39.0	35.0	40.0	29.0	41.0
52-53	36.1014996489212	39.0	35.0	40.0	28.0	41.0
54-55	35.90286384293514	38.5	35.0	40.0	28.0	41.0
56-57	35.70187332066817	38.0	34.0	40.0	28.0	41.0
58-59	35.44241162583911	38.0	34.0	40.0	27.0	41.0
60-61	35.12289850013087	38.0	34.0	40.0	26.0	41.0
62-63	34.79452149713566	37.0	33.5	40.0	26.0	41.0
64-65	34.48091123703199	37.0	33.0	39.5	26.0	41.0
66-67	34.14166870110826	36.0	33.0	39.0	25.5	40.5
68-69	33.74075990393814	36.0	32.0	39.0	25.0	40.0
70-71	33.356617523463854	35.0	32.0	38.5	24.0	40.0
72-73	32.949815329467235	35.0	31.0	38.0	23.5	40.0
74-75	32.54055301480756	35.0	31.0	37.0	22.5	39.0
76-77	31.007859379397203	33.5	29.5	35.5	21.0	38.0
78-79	31.847930470102078	34.0	31.0	36.0	22.0	38.5
80-81	31.822957412078726	34.0	31.0	36.0	22.0	37.5
82-83	31.542840642226846	34.0	31.0	35.0	21.5	37.0
84-85	31.240623817779017	34.0	30.0	35.0	20.0	37.0
86-87	30.945007245450252	34.0	30.0	35.0	20.0	36.0
88-89	30.667899711987666	34.0	30.0	35.0	19.5	36.0
90-91	30.377036392844772	34.0	29.0	35.0	19.0	35.5
92-93	30.162860487024034	34.0	29.0	35.0	18.5	35.0
94-95	29.93757278664377	34.0	29.0	35.0	18.0	35.0
96-97	29.73098379429645	34.0	29.0	35.0	18.0	35.0
98-99	29.56595495709616	33.5	29.0	35.0	17.0	35.0
100	27.65476350735871	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2435925627727329
1101	2	0.2200744423351111
1101	3	0.2882133278319756
1101	4	0.1396302128776341
1101	5	0.1578664912476455
1101	6	0.15893418901196554
1101	7	0.14342656440256007
1101	8	0.182780404840436
1101	9	0.22701893899677117
1101	10-11	0.20502490050612465
1101	12-13	0.1757824904355516
1101	14-15	0.2807005403866185
1101	16-17	0.30418824460542027
1101	18-19	0.3045174198699314
1101	20-21	0.22940703814645502
1101	22-23	0.21831710711893493
1101	24-25	0.18882388429415897
1101	26-27	0.15196506889369488
1101	28-29	0.20252702952071644
1101	30-31	0.3042900311245873
1101	32-33	0.3601536123748659
1101	34-35	0.31240843923931294
1101	36-37	0.3537719706176574
1101	38-39	0.3108271244861598
1101	40-41	0.2614111734136202
1101	42-43	0.3458452164602903
1101	44-45	0.2626092918434395
1101	46-47	0.3465444141413627
1101	48-49	0.3398572453537909
1101	50-51	0.30213667894027907
1101	52-53	0.2821522866091115
1101	54-55	0.22117956808919814
1101	56-57	0.20271005288957156
1101	58-59	0.29459511666678395
1101	60-61	0.19530874356585315
1101	62-63	0.24953022751751774
1101	64-65	0.18625492533757182
1101	66-67	0.12490281842732998
1101	68-69	0.19498448273984081
1101	70-71	0.23871588282871414
1101	72-73	0.0912941936894569
1101	74-75	0.181466681840611
1101	76-77	0.2496642144276997
1101	78-79	0.26858062735460564
1101	80-81	0.1942841376111133
1101	82-83	0.15490087776721495
1101	84-85	0.18261878513548524
1101	86-87	0.07322043536788314
1101	88-89	0.05877034054972441
1101	90-91	0.11651638608698178
1101	92-93	0.03316659949286915
1101	94-95	-0.02301992571770839
1101	96-97	-0.11275857008876145
1101	98-99	-0.2192020707481781
1101	100	-0.13112400848305583
1102	1	0.30020074881078784
1102	2	0.25280283977318163
1102	3	0.30392511274470024
1102	4	0.185004822565773
1102	5	0.19806903459251402
1102	6	0.1779405169571575
1102	7	0.16772494051367204
1102	8	0.17307944621332894
1102	9	0.21901428324343897
1102	10-11	0.23518272617381086
1102	12-13	0.2072014066658454
1102	14-15	0.2957747827644681
1102	16-17	0.30174355595588764
1102	18-19	0.26909322511387757
1102	20-21	0.27251413301761573
1102	22-23	0.21631344777451034
1102	24-25	0.2351684313529958
1102	26-27	0.27074063247441416
1102	28-29	0.3502506189182384
1102	30-31	0.34314559472689865
1102	32-33	0.3553156606743286
1102	34-35	0.37857652601686453
1102	36-37	0.3960769778995257
1102	38-39	0.4289910100789456
1102	40-41	0.3917787413355356
1102	42-43	0.3536830846658887
1102	44-45	0.3786927337719064
1102	46-47	0.3981971283406125
1102	48-49	0.40123669842282084
1102	50-51	0.3725101656956724
1102	52-53	0.33041177120928467
1102	54-55	0.3400982971640403
1102	56-57	0.3056769491021285
1102	58-59	0.3249689635699795
1102	60-61	0.2944963472516591
1102	62-63	0.321854059603389
1102	64-65	0.3804075515231702
1102	66-67	0.37605122791618584
1102	68-69	0.31503511452945077
1102	70-71	0.28103589626883974
1102	72-73	0.3228116818325688
1102	74-75	0.2693159358423287
1102	76-77	0.347867801931411
1102	78-79	0.3413068773945689
1102	80-81	0.25986405042836935
1102	82-83	0.25433498710890134
1102	84-85	0.20461282232890454
1102	86-87	0.20142290128771378
1102	88-89	0.24565883960072554
1102	90-91	0.21106870551298584
1102	92-93	0.18768339242933152
1102	94-95	0.17364198304133893
1102	96-97	0.16166184486894863
1102	98-99	0.2322495170922103
1102	100	0.2080061981648953
1103	1	0.2894088921429443
1103	2	0.25038300863628393
1103	3	0.3315614227756072
1103	4	0.15429959408880478
1103	5	0.16004932887708634
1103	6	0.17908289841019354
1103	7	0.21508959041017306
1103	8	0.18497464276372
1103	9	0.21646793163919398
1103	10-11	0.23583120292844484
1103	12-13	0.27921271847753815
1103	14-15	0.31930735225337514
1103	16-17	0.34782733027919477
1103	18-19	0.34117283423414335
1103	20-21	0.3423522098848011
1103	22-23	0.3044218094002602
1103	24-25	0.2486292593273589
1103	26-27	0.3309122064392298
1103	28-29	0.38150951859856974
1103	30-31	0.46585989902917646
1103	32-33	0.4373276183170418
1103	34-35	0.4125043291643138
1103	36-37	0.46154519724706944
1103	38-39	0.48597233484389335
1103	40-41	0.5306090735219797
1103	42-43	0.49301329141729155
1103	44-45	0.48044854954127914
1103	46-47	0.5654056621563086
1103	48-49	0.5960500478313762
1103	50-51	0.5887891370171161
1103	52-53	0.5328603085851995
1103	54-55	0.5503290643909367
1103	56-57	0.5704728224047315
1103	58-59	0.627951020457651
1103	60-61	0.6178750977016065
1103	62-63	0.6143235037233339
1103	64-65	0.6246261936797382
1103	66-67	0.579029930145893
1103	68-69	0.6253054796098567
1103	70-71	0.6586478485658063
1103	72-73	0.6043699058732486
1103	74-75	0.5510076847676473
1103	76-77	0.6028549300850408
1103	78-79	0.6244482523823685
1103	80-81	0.6041901467909696
1103	82-83	0.6172201096451566
1103	84-85	0.5514018278427564
1103	86-87	0.47020118437851366
1103	88-89	0.5146427947702854
1103	90-91	0.5462168104399083
1103	92-93	0.5624747014975995
1103	94-95	0.5479909114299453
1103	96-97	0.5200207231255938
1103	98-99	0.5696819948486898
1103	100	0.6654727984575004
1104	1	0.26450699689016943
1104	2	0.24984951379424558
1104	3	0.2972847906992868
1104	4	0.09239422684501619
1104	5	0.1630324820795792
1104	6	0.17563825384986842
1104	7	0.21041446152871401
1104	8	0.22376593577956072
1104	9	0.25677050132460266
1104	10-11	0.27829130179411266
1104	12-13	0.2611353038996356
1104	14-15	0.39313611947195426
1104	16-17	0.33711089136337336
1104	18-19	0.3304109683413827
1104	20-21	0.2342940164139904
1104	22-23	0.3613015617762443
1104	24-25	0.31405395616136644
1104	26-27	0.39985323271827156
1104	28-29	0.47162681737192713
1104	30-31	0.4282746909237076
1104	32-33	0.38188804182496483
1104	34-35	0.45831904168509396
1104	36-37	0.4143956590729587
1104	38-39	0.4348728798720316
1104	40-41	0.5778856001418688
1104	42-43	0.6004504282868908
1104	44-45	0.48501795649276147
1104	46-47	0.47970909057353595
1104	48-49	0.5199437987254427
1104	50-51	0.4465729632291655
1104	52-53	0.49459815621838743
1104	54-55	0.5781757747078586
1104	56-57	0.579829292857653
1104	58-59	0.5725206037008519
1104	60-61	0.5480345323191997
1104	62-63	0.5470459915690284
1104	64-65	0.6534055774467333
1104	66-67	0.6095501415132816
1104	68-69	0.6429329520613578
1104	70-71	0.5778750727645843
1104	72-73	0.6275029102207483
1104	74-75	0.6531206259496187
1104	76-77	0.5147213379214826
1104	78-79	0.6456068426051296
1104	80-81	0.7661096279920656
1104	82-83	0.5555278589086363
1104	84-85	0.632160983515881
1104	86-87	0.6463734526082732
1104	88-89	0.636937181546454
1104	90-91	0.5146501059554822
1104	92-93	0.43817077500317225
1104	94-95	0.5791965630450129
1104	96-97	0.5622524392585468
1104	98-99	0.6037416606739363
1104	100	0.8637701284557338
1105	1	0.30477083000112515
1105	2	0.22545960607880033
1105	3	0.269610902394696
1105	4	0.15652952224721162
1105	5	0.10838550722033347
1105	6	0.17488696479978216
1105	7	0.15960901041421494
1105	8	0.18796026505938812
1105	9	0.19664188721595366
1105	10-11	0.21072281717095365
1105	12-13	0.2274182459607914
1105	14-15	0.28176815920905085
1105	16-17	0.33058353302948973
1105	18-19	0.3172401291229221
1105	20-21	0.300936064360549
1105	22-23	0.3659110804454997
1105	24-25	0.3481697986378194
1105	26-27	0.3243623348232916
1105	28-29	0.3828548461324388
1105	30-31	0.4136913515391143
1105	32-33	0.4523145160413691
1105	34-35	0.5139266747381797
1105	36-37	0.4280750944672178
1105	38-39	0.5194478478355649
1105	40-41	0.49184221398259353
1105	42-43	0.4800978938481819
1105	44-45	0.4333170863525879
1105	46-47	0.5087493926593964
1105	48-49	0.587300168033714
1105	50-51	0.5900311126812596
1105	52-53	0.583295188672416
1105	54-55	0.602508937021625
1105	56-57	0.5895971331339993
1105	58-59	0.6429905061492747
1105	60-61	0.6346452844769956
1105	62-63	0.5918774026265794
1105	64-65	0.58412535682357
1105	66-67	0.5691936603337595
1105	68-69	0.5703327865693382
1105	70-71	0.6393444961293753
1105	72-73	0.6748466101740931
1105	74-75	0.6898412997684815
1105	76-77	0.662033562528709
1105	78-79	0.822170287564159
1105	80-81	0.7262048919646844
1105	82-83	0.6980355250830108
1105	84-85	0.6576292827108752
1105	86-87	0.6833064617877902
1105	88-89	0.6783590010907936
1105	90-91	0.704825564286601
1105	92-93	0.7209596403002223
1105	94-95	0.8094511656762506
1105	96-97	0.7365813106799131
1105	98-99	0.7413794324271521
1105	100	0.9047723823404894
1106	1	0.2421367553777003
1106	2	0.17159583090311514
1106	3	0.20906855600728136
1106	4	0.1410443096685441
1106	5	0.10334311432758625
1106	6	0.1406071547245844
1106	7	0.18441121753904355
1106	8	0.1404743119151206
1106	9	0.17462417506207828
1106	10-11	0.15716204384658994
1106	12-13	0.1973213555883504
1106	14-15	0.2891152721427446
1106	16-17	0.2402194741140491
1106	18-19	0.31779608129131987
1106	20-21	0.29869874846031763
1106	22-23	0.2598075475093822
1106	24-25	0.2735379189607201
1106	26-27	0.2850358289017123
1106	28-29	0.3331767976987763
1106	30-31	0.35271345648947516
1106	32-33	0.3697766850274178
1106	34-35	0.40769554910750827
1106	36-37	0.4045113900306774
1106	38-39	0.39663044626871624
1106	40-41	0.43648107254011137
1106	42-43	0.40305328911406946
1106	44-45	0.4014790396717913
1106	46-47	0.546489446214899
1106	48-49	0.5442091320540712
1106	50-51	0.5168582598661189
1106	52-53	0.5913802713529179
1106	54-55	0.5458292438741381
1106	56-57	0.5855247791007017
1106	58-59	0.6166798189939868
1106	60-61	0.6443339609421272
1106	62-63	0.6270549637001395
1106	64-65	0.7029697242704671
1106	66-67	0.688855676965332
1106	68-69	0.6560971376456521
1106	70-71	0.6866357076237719
1106	72-73	0.6518209861292164
1106	74-75	0.6759498079397375
1106	76-77	0.5869930782466781
1106	78-79	0.6947445194539306
1106	80-81	0.7490483752382033
1106	82-83	0.6918297244023357
1106	84-85	0.6618385041076955
1106	86-87	0.6253357769573213
1106	88-89	0.6727339319597512
1106	90-91	0.6329055045415402
1106	92-93	0.784324196299206
1106	94-95	0.7799014807730096
1106	96-97	0.8273984380544626
1106	98-99	0.8438556634480712
1106	100	1.0120295116262916
1107	1	0.2514638751236191
1107	2	0.23155457186537376
1107	3	0.3027562814532274
1107	4	0.17997943653425352
1107	5	0.17047808672570142
1107	6	0.1702209151877767
1107	7	0.1698267559331299
1107	8	0.18304710011042857
1107	9	0.23042917803226715
1107	10-11	0.22786483691182724
1107	12-13	0.23330207903767786
1107	14-15	0.2584566992396944
1107	16-17	0.3384586660054225
1107	18-19	0.2665432309451319
1107	20-21	0.3360352815909451
1107	22-23	0.41302855681225026
1107	24-25	0.433535372710395
1107	26-27	0.44746342804541683
1107	28-29	0.38957183506533255
1107	30-31	0.3299341401566309
1107	32-33	0.315459985782816
1107	34-35	0.37423409509195693
1107	36-37	0.4457127975694277
1107	38-39	0.4362509071776799
1107	40-41	0.3990122476999076
1107	42-43	0.4178939504445367
1107	44-45	0.38928583121040816
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2316	72-73	-0.32080682910664393
2316	74-75	-0.3762502542423576
2316	76-77	-0.39860812906379195
2316	78-79	-0.2960720874420453
2316	80-81	-0.183425386895113
2316	82-83	-0.0927683420493075
2316	84-85	-0.21333728216363212
2316	86-87	-0.17625285350046838
2316	88-89	-0.1794283137185282
2316	90-91	-0.059243611860836154
2316	92-93	0.07648107241783464
2316	94-95	0.15362904757123275
2316	96-97	0.14564843158803598
2316	98-99	-0.0232939598329871
2316	100	-0.18605811711250553
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	32.0
12	79.0
13	219.0
14	716.0
15	1843.0
16	4166.0
17	8720.0
18	15579.0
19	23910.0
20	33328.0
21	45167.0
22	58174.0
23	66357.0
24	70689.0
25	77315.0
26	89172.0
27	105885.0
28	128568.0
29	154062.0
30	186692.0
31	225887.0
32	277601.0
33	349729.0
34	459035.0
35	624478.0
36	907633.0
37	1378651.0
38	1318795.0
39	199347.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3207140929776	20.16297931400589	13.663405494870414	23.85290109814609
2	15.041519909710777	21.51261476936248	40.003513793770566	23.442351527156177
3	17.338457565727325	27.323003539290617	30.68905110046262	24.649487794519438
4	12.00682225995729	17.46897561429627	38.25031170088342	32.27389042486302
5	12.391668998417737	38.58034088683662	35.33288450183667	13.695105612908964
6	30.44263405977734	38.02188022805092	18.112155767070494	13.423329945101244
7	26.661407213094922	33.076908057871414	22.931398629537508	17.33028609949616
8	25.70717410021881	36.29740765833382	20.478845884234627	17.516572357212745
9	26.74703146206136	15.261202712036589	20.44312452334937	37.54864130255268
10-11	24.537765817593755	26.55447608223362	29.093077501141025	19.8146805990316
12-13	25.059590910618994	24.347854426272058	29.095734631108883	21.496820032000066
14-15	22.34248467489281	25.68328977940096	26.702548686768562	25.27167685893767
16-17	21.950220888330314	28.537857689281843	27.346721056494065	22.165200365893778
18-19	21.74664139185199	27.62625815911022	29.34147608098462	21.28562436805317
20-21	22.56051674861429	26.90114961563012	29.318898102491197	21.219435533264395
22-23	22.3300668003991	26.779247754706663	29.11979040087506	21.770895044019177
24-25	22.003560261596725	26.83575071063567	29.303579243199252	21.857109784568355
26-27	21.944604072209707	26.977976408627047	29.23929159085826	21.83812792830499
28-29	21.991725245137005	27.09760543574146	29.263757334144863	21.646911984976665
30-31	22.40054077158016	26.909529909978325	29.264343802475974	21.425585515965544
32-33	22.117315412673634	27.040282727220145	29.21007878556368	21.63232307454254
34-35	22.236545889086813	27.158773442923334	29.146149188355313	21.458531479634544
36-37	22.25415631540836	27.11932907330654	29.041251744681723	21.58526286660337
38-39	22.199585731420985	27.296047888913368	28.960858820060498	21.543507559605153
40-41	22.232204550507845	27.082265436674824	29.05129376039559	21.634236252421736
42-43	22.083049188968175	27.194256381044386	28.81846349898002	21.904230931007422
44-45	22.034187696648548	27.393341731403524	28.77809840452571	21.794372167422218
46-47	22.012838493635147	27.264665910384533	28.58761154659892	22.134884049381395
48-49	22.078492275081192	27.36773426508335	28.586413192607722	21.967360267227736
50-51	22.171180167921744	27.569587630379544	28.32703103152012	21.93220117017859
52-53	22.211744156112893	27.5971879372836	28.17726597640622	22.01380193019729
54-55	22.059843726040736	27.51072916323354	28.37624105554399	22.053186055181744
56-57	22.126758743804757	27.61629289690649	28.102700068118292	22.15424829117046
58-59	22.106854243783502	27.56796680502799	28.309147261875783	22.016031689312726
60-61	22.336977191440518	27.690377987423737	28.223446115362734	21.74919870577301
62-63	22.099542258920987	27.67492007168167	28.311960753178727	21.91357691621862
64-65	22.01656987923427	27.405894949575917	28.300386848771353	22.27714832241846
66-67	21.976384072859183	27.541026015222652	28.158416635272136	22.324173276646025
68-69	21.90776467002353	27.57708147681475	28.265304032499667	22.24984982066205
70-71	21.936239615244045	27.394145169340057	28.262428472845098	22.407186742570804
72-73	21.89984627794952	27.721890009711043	27.951808750125434	22.426454962214002
74-75	21.91669248633943	27.71077386128637	27.9359904070465	22.4365432453277
76-77	21.83618313473187	27.561545631921437	28.07491433639619	22.527356896950508
78-79	21.772538392541296	27.54533576585378	28.057534839263454	22.62459100234147
80-81	21.777242014345227	27.764427735359092	27.81118713979843	22.647143110497243
82-83	21.8862473400612	27.695195423370933	27.833571705548426	22.58498553101944
84-85	21.809937257930084	27.762060998039615	27.740741483040964	22.687260260989333
86-87	21.64408085125034	27.785996875201207	27.854620281737002	22.715301991811444
88-89	21.59871320966573	27.598426737446303	27.921262280881237	22.88159777200673
90-91	21.845562607705833	27.554933646744832	27.886876128451725	22.71262761709761
92-93	21.774828981178292	27.539256405427224	27.78737825475444	22.898536358640044
94-95	21.5720653688626	27.477807180388815	27.83849786549184	23.111629585256747
96-97	21.531543199027006	27.40054919536169	27.903667474410554	23.164240131200746
98-99	21.42320269978992	27.366816511428354	27.82074100916747	23.38923977961426
100	20.571880137221633	27.92286069866508	28.010562377286657	23.49469678682663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2708.0
1	2074.0
2	2527.0
3	3508.5
4	2579.5
5	2070.0
6	3195.5
7	6025.0
8	9458.5
9	10953.0
10	9406.0
11	7483.0
12	7470.0
13	8012.5
14	8519.0
15	9083.0
16	9626.0
17	10168.0
18	10921.5
19	11732.5
20	12926.5
21	14919.5
22	17574.5
23	21702.5
24	27209.0
25	33290.5
26	41384.5
27	50693.5
28	60642.5
29	72270.5
30	86353.5
31	100383.5
32	114302.5
33	130028.0
34	145658.0
35	159309.5
36	175694.0
37	192231.0
38	203437.5
39	212304.5
40	216465.5
41	219542.5
42	223822.5
43	228860.0
44	233711.0
45	239290.0
46	243896.0
47	245997.0
48	250259.0
49	253226.5
50	253082.5
51	246971.0
52	235346.0
53	224352.5
54	213456.5
55	198949.0
56	180395.0
57	161152.0
58	142871.5
59	123086.5
60	101474.0
61	82837.0
62	67628.5
63	52890.0
64	39795.0
65	30033.5
66	22393.5
67	16865.5
68	12958.5
69	9746.0
70	7521.5
71	5903.5
72	4637.0
73	3641.5
74	2844.5
75	2172.0
76	1627.0
77	1132.0
78	776.5
79	526.5
80	361.5
81	242.0
82	177.0
83	141.0
84	94.5
85	55.5
86	43.0
87	37.0
88	26.0
89	15.0
90	9.5
91	8.5
92	6.0
93	3.0
94	3.0
95	4.0
96	2.5
97	2.5
98	2.5
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002921374937039966
2	0.06465193579258297
3	6.459321468832086E-4
4	6.165715947521537E-4
5	0.0014533473304872192
6	7.193335272108459E-4
7	0.0
8	9.688982203248128E-4
9	8.808165639316481E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.046532511453185E-4
18-19	0.001079000290816269
20-21	0.002833293280646801
22-23	7.340138032763733E-6
24-25	2.9360552131054933E-5
26-27	9.542179442592854E-5
28-29	8.514560118005932E-4
30-31	0.0016515310573718403
32-33	0.003038817145564186
34-35	0.004264620197035729
36-37	0.0012992044317991808
38-39	0.0014166466403234006
40-41	0.005571164766867674
42-43	0.006987811407191074
44-45	0.005512443662605564
46-47	0.00689972975079791
48-49	0.00869072343079226
50-51	0.008705403706857788
52-53	0.004073776608183872
54-55	0.0024883067931069057
56-57	0.0026571299678604717
58-59	0.003332422666874735
60-61	0.007875968109155487
62-63	0.013425112461924871
64-65	0.004176538540642564
66-67	0.01020279186554159
68-69	0.012294731204879252
70-71	0.005497763386540036
72-73	0.007949369489483124
74-75	0.0028553136947450926
76-77	0.0027525517622864005
78-79	0.007993410317679707
80-81	0.0058280695980144044
82-83	0.015913419255031775
84-85	0.0176089911406002
86-87	0.02205711478845502
88-89	0.020956094083540457
90-91	0.024149054127792686
92-93	0.03381601591694252
94-95	0.03764022783201243
96-97	0.022519543484519136
98-99	0.018453107014368027
100	0.018687991431416466
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6811861.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.7409315803214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3104298022332	44.3937430251949
2	15.594239167609691	18.632287503186774
3	4.860653578853861	8.711399186698584
4	2.0261515351908703	4.8417672094080375
5	1.0058388334335195	3.004487446449109
6	0.5811932690845714	2.0832616393994816
7	0.34944831816488686	1.4613457646442822
8	0.2497609500686278	1.193676146358877
9	0.1765321284352117	0.9491574425918843
>10	0.7816736559029256	7.951375489987518
>50	0.04043289147711081	1.6851245773942864
>100	0.02134661642017638	2.3763361597817356
>500	0.0016230035002766739	0.6532861100983592
>1k	5.74812181259482E-4	0.7550921362920532
>5k	0.0	0.0
>10k+	1.014374437514201E-4	1.307660162514113
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26663	0.3914202007351588	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21938	0.32205589632554155	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16135	0.2368662543172857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.4680276065527467E-5	0.0	0.0	0.0
4	0.0	1.4680276065527467E-5	0.0	0.0	0.0
5	0.0	2.9360552131054933E-5	0.0	0.0	0.0
6	0.0	2.9360552131054933E-5	0.0	0.0	4.4040828196582405E-5
7	0.0	2.9360552131054933E-5	0.0	1.4680276065527467E-5	4.4040828196582405E-5
8	0.0	2.9360552131054933E-5	0.0	1.4680276065527467E-5	4.4040828196582405E-5
9	0.0	2.9360552131054933E-5	0.0	2.9360552131054933E-5	4.4040828196582405E-5
10-11	0.0	2.9360552131054933E-5	0.0	2.9360552131054933E-5	4.4040828196582405E-5
12-13	0.0	2.9360552131054933E-5	0.0	3.670069016381867E-5	4.4040828196582405E-5
14-15	0.0	5.138096622934614E-5	0.0	5.872110426210987E-5	5.872110426210987E-5
16-17	0.0	7.340138032763734E-5	0.0	5.872110426210987E-5	5.872110426210987E-5
18-19	0.0	7.340138032763734E-5	0.0	5.872110426210987E-5	8.808165639316481E-5
20-21	0.0	1.0276193245869228E-4	0.0	7.340138032763734E-5	8.808165639316481E-5
22-23	0.0	1.0276193245869228E-4	1.4680276065527467E-5	1.8350345081909335E-4	1.0276193245869228E-4
24-25	0.0	1.0276193245869228E-4	1.4680276065527467E-5	2.7158510721225814E-4	1.1744220852421973E-4
26-27	0.0	1.0276193245869228E-4	1.4680276065527467E-5	5.138096622934614E-4	1.1744220852421973E-4
28-29	0.0	1.0276193245869228E-4	1.4680276065527467E-5	0.0017983338180271148	1.3212248458974722E-4
30-31	0.0	1.0276193245869228E-4	1.4680276065527467E-5	0.006062954015062844	1.4680276065527468E-4
32-33	0.0	1.0276193245869228E-4	1.4680276065527467E-5	0.015260146970115802	1.4680276065527468E-4
34-35	0.0	1.0276193245869228E-4	1.4680276065527467E-5	0.029184388818268604	1.4680276065527468E-4
36-37	0.0	1.1010207049145601E-4	1.4680276065527467E-5	0.05060291159787318	1.5414289868803842E-4
38-39	0.0	1.2478234655698348E-4	1.4680276065527467E-5	0.08576951291284424	1.6148303672080215E-4
40-41	0.0	1.3212248458974722E-4	1.4680276065527467E-5	0.12797530660123568	1.6148303672080215E-4
42-43	0.0	1.4680276065527468E-4	1.4680276065527467E-5	0.16933698441585934	1.6148303672080215E-4
44-45	0.0	1.4680276065527468E-4	1.4680276065527467E-5	0.21966831090652028	1.7616331278632962E-4
46-47	0.0	1.4680276065527468E-4	1.4680276065527467E-5	0.2818539603200946	1.9818372688462082E-4
48-49	0.0	1.4680276065527468E-4	1.4680276065527467E-5	0.3488474001451292	2.0552386491738456E-4
50-51	0.0	1.4680276065527468E-4	1.4680276065527467E-5	0.42157348777375225	2.0552386491738456E-4
52-53	0.0	1.4680276065527468E-4	1.4680276065527467E-5	0.4989752433292458	2.2754427901567576E-4
54-55	0.0	2.1286400295014826E-4	1.4680276065527467E-5	0.5853686679748751	2.4956469311396696E-4
56-57	0.0	2.3488441704843947E-4	1.4680276065527467E-5	0.6873085049738977	2.569048311467307E-4
58-59	0.0	2.3488441704843947E-4	1.4680276065527467E-5	0.8015871140059962	2.7158510721225814E-4
60-61	0.0	2.3488441704843947E-4	1.4680276065527467E-5	0.927543882648222	2.789252452450219E-4
62-63	0.0	2.3488441704843947E-4	1.4680276065527467E-5	1.0597177482041986	2.9360552131054937E-4
64-65	0.0	2.3488441704843947E-4	1.4680276065527467E-5	1.1927210493578775	2.9360552131054937E-4
66-67	0.0	2.3488441704843947E-4	1.4680276065527467E-5	1.3446765869121522	2.9360552131054937E-4
68-69	0.0	2.422245550812032E-4	1.4680276065527467E-5	1.5263817039132184	2.9360552131054937E-4
70-71	0.0	2.4956469311396696E-4	1.4680276065527467E-5	1.7311054937850319	3.229660734416043E-4
72-73	0.0	2.4956469311396696E-4	1.4680276065527467E-5	1.9308967108988278	4.6976883409687893E-4
74-75	0.0	2.4956469311396696E-4	1.4680276065527467E-5	2.136582058852933	4.6976883409687893E-4
76-77	0.0	2.4956469311396696E-4	1.4680276065527467E-5	2.3600672415364907	4.771089721296427E-4
78-79	0.0	2.4956469311396696E-4	1.4680276065527467E-5	2.618344678495348	4.844491101624064E-4
80-81	0.0	2.4956469311396696E-4	1.4680276065527467E-5	2.9004482035085566	4.917892481951702E-4
82-83	0.0	2.4956469311396696E-4	1.4680276065527467E-5	3.1962998070571316	5.138096622934613E-4
84-85	0.0	2.4956469311396696E-4	1.4680276065527467E-5	3.494316751325372	5.138096622934613E-4
86-87	0.0	2.4956469311396696E-4	1.4680276065527467E-5	3.8001289221844075	5.138096622934613E-4
88	0.0	2.4956469311396696E-4	1.4680276065527467E-5	4.049392669639031	5.138096622934613E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12350	0.0	48.363987	1
GTATCAA	21550	0.0	37.908615	1
TACCTGG	5840	0.0	30.038494	2
TCAACGC	26835	0.0	30.032444	4
ATCAACG	27055	0.0	29.736124	3
CAACGCA	27330	0.0	29.522453	5
TATCAAC	28070	0.0	28.851799	2
AACGCAG	28335	0.0	28.673927	6
GTACCTG	6590	0.0	27.476452	1
GTACATG	26110	0.0	26.244482	1
ACGCAGA	32365	0.0	25.030933	7
TACATGG	26845	0.0	24.91256	2
CGCAGAG	32870	0.0	24.57489	8
TATAACG	725	0.0	24.001915	2
CATGGGG	15695	0.0	23.98277	4
ACATGGG	26615	0.0	23.942053	3
ACCTGGG	7230	0.0	23.528727	3
ATGGGGG	9525	0.0	23.039534	5
GCAGAGT	36490	0.0	21.750437	9
TAACGCA	805	0.0	21.01526	4
>>END_MODULE