FastQCFastQC Report
Mon 6 Jun 2016
ERR1211552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3920661
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154640.3944232872977287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140840.359225140862727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90180.23001223518177163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53050.1353088165490462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79350.048.0184061
TACCTGG37800.040.1827622
GTACCTG41350.037.5875931
GTATCAA135650.035.6927031
ACCTGGG43900.033.8274233
TCAACGC163400.028.9314424
ATCAACG164200.028.8205763
CAACGCA163900.028.75245
AACGCAG170000.027.9066836
TATCAAC170600.027.7850112
GTACATG141700.027.3217241
TATCACG2850.026.4000172
TACATGG142600.026.2825722
ACGCAGA189600.024.9986367
ACATGGG143350.024.9150773
CGCAGAG194650.024.2315718
CATGGGG85100.022.9714264
ATGGGGG57200.022.3421545
GCAGAGT210850.021.7924619
GAGTACT134050.021.45128612-13
Produced by FastQC (version 0.11.5)