##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211552_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3920661
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.378171946006045	31.0	31.0	34.0	28.0	34.0
2	31.410754462066475	31.0	31.0	34.0	28.0	34.0
3	31.505968508881537	33.0	31.0	34.0	28.0	34.0
4	35.050650132719966	37.0	35.0	37.0	32.0	37.0
5	35.13737045870582	37.0	35.0	37.0	32.0	37.0
6	35.253021620588974	37.0	35.0	37.0	32.0	37.0
7	35.188487604513625	37.0	35.0	37.0	32.0	37.0
8	35.159498360097956	37.0	35.0	37.0	32.0	37.0
9	36.736669658509115	39.0	37.0	39.0	32.0	39.0
10-11	36.72449722125938	39.0	37.0	39.0	32.0	39.0
12-13	36.667875008831416	39.0	37.0	39.0	32.0	39.0
14-15	37.85649958004531	40.0	38.0	41.0	32.0	41.0
16-17	37.83542736798718	40.0	38.0	41.0	32.0	41.0
18-19	37.85095599951131	40.0	38.0	41.0	32.0	41.0
20-21	37.807531689171796	40.0	38.0	41.0	32.0	41.0
22-23	37.6597812205646	40.0	37.0	41.0	32.0	41.0
24-25	37.5791890704144	40.0	37.0	41.0	32.0	41.0
26-27	37.435665057499236	40.0	37.0	41.0	31.0	41.0
28-29	37.26764976620014	40.0	37.0	41.0	31.0	41.0
30-31	37.077453776289246	40.0	36.0	41.0	30.0	41.0
32-33	36.89079379727041	39.0	36.0	41.0	30.0	41.0
34-35	36.713656829804975	39.0	35.5	41.0	30.0	41.0
36-37	36.53700868297463	39.0	35.0	41.0	30.0	41.0
38-39	36.400639713558505	39.0	35.0	41.0	29.0	41.0
40-41	36.3589725048914	39.0	35.0	41.0	29.0	41.0
42-43	36.29595532488017	39.0	35.0	41.0	29.0	41.0
44-45	36.10733700261257	39.0	35.0	40.5	28.0	41.0
46-47	36.03832006898837	39.0	35.0	40.5	27.5	41.0
48-49	35.99536340938428	39.0	35.0	41.0	27.0	41.0
50-51	35.01050486129762	38.0	34.0	40.0	25.5	40.5
52-53	35.2102427371303	38.5	34.5	39.5	26.0	40.5
54-55	35.7965873611618	39.0	35.0	40.5	27.0	41.0
56-57	35.76198465003733	39.0	35.0	41.0	26.5	41.0
58-59	35.60688618577326	39.0	35.0	41.0	26.0	41.0
60-61	35.42223109827654	38.0	34.0	40.0	26.0	41.0
62-63	35.15742217957635	38.0	34.0	40.0	26.0	41.0
64-65	34.883863588308195	37.5	34.0	40.0	26.0	41.0
66-67	34.5822390663207	37.0	34.0	40.0	25.5	41.0
68-69	34.297390924642556	36.5	33.0	39.5	25.0	41.0
70-71	33.949747504311134	36.0	33.0	39.0	24.5	41.0
72-73	33.5996793652907	35.5	33.0	39.0	24.0	40.5
74-75	33.23470787196343	35.0	32.5	37.5	24.0	40.0
76-77	32.89121120137651	35.0	32.0	37.0	23.0	39.0
78-79	32.56610339430009	35.0	32.0	37.0	23.0	39.0
80-81	32.23119315850057	35.0	32.0	36.0	22.5	39.0
82-83	31.927716015233145	35.0	31.0	36.0	21.5	37.0
84-85	31.658783939748936	35.0	31.0	35.0	20.5	37.0
86-87	31.366376103417252	35.0	31.0	35.0	20.0	36.5
88-89	31.1425504525895	34.0	31.0	35.0	20.0	36.0
90-91	30.908561464508153	34.0	30.5	35.0	19.5	36.0
92-93	30.753497943331496	34.0	30.0	35.0	19.0	36.0
94-95	30.590889775984202	34.0	30.0	35.0	18.5	35.0
96-97	30.486444250089463	34.0	30.0	35.0	18.0	35.0
98-99	30.313482598980123	34.0	30.0	35.0	18.0	35.0
100	28.944854706897637	33.0	27.0	35.0	13.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4461086270018235
1101	2	0.44337214824999194
1101	3	0.4166461668364363
1101	4	0.3601047558206787
1101	5	0.34764843213355334
1101	6	0.41302564538156616
1101	7	0.4329613640378369
1101	8	0.3978951585277457
1101	9	0.43558031955811316
1101	10-11	0.4814380724616285
1101	12-13	0.4253173572734852
1101	14-15	0.5044636572344743
1101	16-17	0.562140034249822
1101	18-19	0.468273968720041
1101	20-21	0.4305551543567958
1101	22-23	0.5129354449934667
1101	24-25	0.46335280984794736
1101	26-27	0.47744325000984844
1101	28-29	0.5544300402842239
1101	30-31	0.5918488010272966
1101	32-33	0.42795610834372866
1101	34-35	0.4810541731576805
1101	36-37	0.46580614397520037
1101	38-39	0.4171093322045323
1101	40-41	0.42484921176250623
1101	42-43	0.2590873374910032
1101	44-45	0.19211524271035785
1101	46-47	0.18591643704508698
1101	48-49	0.2834354940923802
1101	50-51	0.26439230859967466
1101	52-53	0.512190780001859
1101	54-55	0.5646169608151723
1101	56-57	0.5383214051095422
1101	58-59	0.4446115868391942
1101	60-61	0.3655122691526316
1101	62-63	0.3490653987608212
1101	64-65	0.46970436283617545
1101	66-67	0.4331149191981041
1101	68-69	0.32580759991260777
1101	70-71	0.2755589835780583
1101	72-73	0.29523447276842774
1101	74-75	0.2366136476235141
1101	76-77	0.1511421308294274
1101	78-79	0.07755136656795258
1101	80-81	0.07638143234137118
1101	82-83	0.02211878101585185
1101	84-85	-0.054672956083713586
1101	86-87	0.01113683773422025
1101	88-89	-0.009642841440747674
1101	90-91	-0.16386525072315195
1101	92-93	-0.17669567424898602
1101	94-95	-0.17971123373773068
1101	96-97	-0.18001804524416443
1101	98-99	-0.3263320545646984
1101	100	-0.352546939728704
1102	1	0.27523027416919277
1102	2	0.3248279878065681
1102	3	0.2937086813611849
1102	4	0.2685275500531006
1102	5	0.3207022890400495
1102	6	0.2881228528140767
1102	7	0.3147772439688836
1102	8	0.30915387771359804
1102	9	0.2585035837854335
1102	10-11	0.2497718624935743
1102	12-13	0.261054312747099
1102	14-15	0.266605654660772
1102	16-17	0.2481210055263645
1102	18-19	0.32591095228929134
1102	20-21	0.32513061542006483
1102	22-23	0.3312492905923392
1102	24-25	0.3350303274424107
1102	26-27	0.27786017537604835
1102	28-29	0.25840227763337964
1102	30-31	0.321857715321741
1102	32-33	0.2642296557819961
1102	34-35	0.3445891591618917
1102	36-37	0.26380580405268006
1102	38-39	0.1590727662543543
1102	40-41	0.1993095847754418
1102	42-43	0.16416120126532263
1102	44-45	0.09990467112024248
1102	46-47	0.1095823279022099
1102	48-49	0.12525706252259994
1102	50-51	0.16983072192464022
1102	52-53	0.26702028111354537
1102	54-55	0.22915645252696493
1102	56-57	0.2591593487864614
1102	58-59	0.2703690417214162
1102	60-61	0.14180406141141333
1102	62-63	0.181740623612626
1102	64-65	0.2693738055366808
1102	66-67	0.17726148823533094
1102	68-69	0.09852188116727234
1102	70-71	0.03645540780134837
1102	72-73	0.11657640359766219
1102	74-75	0.09822668399868917
1102	76-77	0.0734379258492126
1102	78-79	-0.009480936476435886
1102	80-81	0.09102515539471057
1102	82-83	0.0461093325574673
1102	84-85	-0.053199842913798534
1102	86-87	-0.17683302709455262
1102	88-89	-0.11782005900155923
1102	90-91	-0.11186473737078728
1102	92-93	-0.18598076170966138
1102	94-95	-0.19726094740504507
1102	96-97	-0.2555526427655046
1102	98-99	-0.2818649871594552
1102	100	-0.1747015628482913
1103	1	0.37826041559410584
1103	2	0.3412948664065638
1103	3	0.27006958866638797
1103	4	0.31582251455165533
1103	5	0.2820909409225436
1103	6	0.25137601638414964
1103	7	0.2949399019336383
1103	8	0.3393444643449115
1103	9	0.316712289930706
1103	10-11	0.3537731883638031
1103	12-13	0.2270182642742853
1103	14-15	0.31653354991339455
1103	16-17	0.28596096884317745
1103	18-19	0.38926584855886404
1103	20-21	0.3689147651149369
1103	22-23	0.4451047627220035
1103	24-25	0.351089911875178
1103	26-27	0.4093690869799076
1103	28-29	0.31527760570102714
1103	30-31	0.31270580683435867
1103	32-33	0.33079809875603416
1103	34-35	0.3934776580819701
1103	36-37	0.5230684466437197
1103	38-39	0.3209776482643605
1103	40-41	0.2921132066262615
1103	42-43	0.26181208860499083
1103	44-45	0.28012533991523014
1103	46-47	0.3345324140607957
1103	48-49	0.411661917734115
1103	50-51	0.36961812954536555
1103	52-53	0.36013632035464127
1103	54-55	0.4724431657650996
1103	56-57	0.39041646688258425
1103	58-59	0.3396025041594797
1103	60-61	0.33796969301902635
1103	62-63	0.3397662183799355
1103	64-65	0.3163661315524209
1103	66-67	0.296559991095279
1103	68-69	0.2443518157068354
1103	70-71	0.16792515033875333
1103	72-73	0.18874848116182363
1103	74-75	0.19381921819294234
1103	76-77	0.208926558884599
1103	78-79	0.16548493219754334
1103	80-81	0.2493746564326358
1103	82-83	0.27269091987934146
1103	84-85	0.1097725965447438
1103	86-87	0.04649418792016391
1103	88-89	0.21345477868861806
1103	90-91	0.17609154417988293
1103	92-93	0.04931241608097636
1103	94-95	0.0865254851036319
1103	96-97	-0.018421003655493706
1103	98-99	-0.06756765803557485
1103	100	-0.0020083015789147396
1104	1	0.3454538393379174
1104	2	0.3798519554168891
1104	3	0.3701522508974371
1104	4	0.3639806631294604
1104	5	0.3036191761921785
1104	6	0.30180564238374785
1104	7	0.20880509478102738
1104	8	0.31125598330271487
1104	9	0.4095812125585283
1104	10-11	0.3900429166055588
1104	12-13	0.3564005921555946
1104	14-15	0.40600266863701506
1104	16-17	0.4466195389914773
1104	18-19	0.3643797414205068
1104	20-21	0.3241429725410683
1104	22-23	0.37904383219188986
1104	24-25	0.4807456980433997
1104	26-27	0.48789476508365937
1104	28-29	0.42073262467183525
1104	30-31	0.3378801702443752
1104	32-33	0.3504565993882096
1104	34-35	0.38647887310505524
1104	36-37	0.400659358023276
1104	38-39	0.3268138252020947
1104	40-41	0.27494412940582436
1104	42-43	0.05673870410151238
1104	44-45	0.1882036206721267
1104	46-47	0.27022722868281335
1104	48-49	0.20510563750380584
1104	50-51	0.35137232024727894
1104	52-53	0.4585251824859711
1104	54-55	0.42007915990667755
1104	56-57	0.5029824337307574
1104	58-59	0.46306219675212645
1104	60-61	0.5308207362928314
1104	62-63	0.48206464560564655
1104	64-65	0.39976746767013793
1104	66-67	0.3006592975913023
1104	68-69	0.27469030518043525
1104	70-71	0.23049817733456024
1104	72-73	0.4098522128111952
1104	74-75	0.2959675626161129
1104	76-77	0.27090012190101476
1104	78-79	0.16915766494376783
1104	80-81	0.26086106926188535
1104	82-83	0.33802402251588504
1104	84-85	0.13962762341486723
1104	86-87	0.0816038494582223
1104	88-89	0.13864246822657833
1104	90-91	0.1618184246318073
1104	92-93	0.056050899476549176
1104	94-95	0.15605367081722932
1104	96-97	0.017327353728489925
1104	98-99	-0.00933165829246363
1104	100	0.044179994332282035
1105	1	0.42903591961271914
1105	2	0.4494159878764421
1105	3	0.3930339866033563
1105	4	0.37484988412021636
1105	5	0.37486305093550243
1105	6	0.32122416692504885
1105	7	0.29523699649120516
1105	8	0.32354312214319236
1105	9	0.4160508210988496
1105	10-11	0.4085792680785758
1105	12-13	0.40909053948188046
1105	14-15	0.5317359351460382
1105	16-17	0.4672304145033692
1105	18-19	0.45787267147022703
1105	20-21	0.44551539548837127
1105	22-23	0.4907592384338457
1105	24-25	0.49293089105003673
1105	26-27	0.4016088294451592
1105	28-29	0.48987770128285746
1105	30-31	0.5386106061154692
1105	32-33	0.5306893852648287
1105	34-35	0.5573680620829364
1105	36-37	0.5490796584315234
1105	38-39	0.5362836313110222
1105	40-41	0.5778010615965954
1105	42-43	0.5854505626284521
1105	44-45	0.6286928138510888
1105	46-47	0.5445073638311086
1105	48-49	0.6065713854987465
1105	50-51	0.6233005208012301
1105	52-53	0.5806679729918187
1105	54-55	0.5836184963429929
1105	56-57	0.6486372290082514
1105	58-59	0.6513953494140452
1105	60-61	0.7454519755721094
1105	62-63	0.6788441646581589
1105	64-65	0.7313077242714243
1105	66-67	0.598971401452701
1105	68-69	0.632937771364297
1105	70-71	0.681083249967422
1105	72-73	0.5898881266807052
1105	74-75	0.5317981768133748
1105	76-77	0.5181291644766404
1105	78-79	0.5861441235898468
1105	80-81	0.5597092126232965
1105	82-83	0.5235080235573726
1105	84-85	0.4667402425086351
1105	86-87	0.5221758853489433
1105	88-89	0.5122945527362184
1105	90-91	0.49769637599048977
1105	92-93	0.41673124069206935
1105	94-95	0.3884402841423338
1105	96-97	0.32935131959986563
1105	98-99	0.5035976991122695
1105	100	0.527313705844918
1106	1	0.3631754124579949
1106	2	0.33322351306327036
1106	3	0.2797223628521408
1106	4	0.29737765770648394
1106	5	0.3573966953253773
1106	6	0.33923267552096803
1106	7	0.3882462309639294
1106	8	0.38606674063466073
1106	9	0.38108605031974463
1106	10-11	0.3732979710203139
1106	12-13	0.3150251817543932
1106	14-15	0.40059674958860825
1106	16-17	0.38601610069670045
1106	18-19	0.4334962161422311
1106	20-21	0.3570040951262641
1106	22-23	0.4910993007184814
1106	24-25	0.44953132182492794
1106	26-27	0.5263874920105494
1106	28-29	0.5065766071445523
1106	30-31	0.6049976489065614
1106	32-33	0.519798318696644
1106	34-35	0.5086202380636351
1106	36-37	0.519726740208803
1106	38-39	0.4870355571941616
1106	40-41	0.4716327254866073
1106	42-43	0.5617040253907462
1106	44-45	0.572549130057098
1106	46-47	0.5385353224059841
1106	48-49	0.5955722347746004
1106	50-51	0.5531105159488519
1106	52-53	0.609523170687666
1106	54-55	0.5598417692291093
1106	56-57	0.6236496623980585
1106	58-59	0.5416030183684697
1106	60-61	0.5186798958294858
1106	62-63	0.5439345440189669
1106	64-65	0.6765112293325402
1106	66-67	0.5757221173211988
1106	68-69	0.5417457584727217
1106	70-71	0.5245451401154213
1106	72-73	0.39815145322892675
1106	74-75	0.5899180052160915
1106	76-77	0.5726312118891528
1106	78-79	0.48526003313767774
1106	80-81	0.5107799194029425
1106	82-83	0.5170700462108861
1106	84-85	0.4824988354602411
1106	86-87	0.5260274041691702
1106	88-89	0.5629958102187977
1106	90-91	0.5945652953974907
1106	92-93	0.6200643185021022
1106	94-95	0.6848803423356564
1106	96-97	0.544250254452642
1106	98-99	0.5981151076176978
1106	100	0.44674347022530725
1107	1	0.22857642017376634
1107	2	0.22600194405623952
1107	3	0.2554852418718312
1107	4	0.21832145899664113
1107	5	0.14504441587694572
1107	6	0.12013333487182365
1107	7	0.20880395382603467
1107	8	0.16608277166545804
1107	9	0.13646217737615274
1107	10-11	0.22142525312273875
1107	12-13	0.22832497139729213
1107	14-15	0.18892408280834871
1107	16-17	0.22972999853880793
1107	18-19	0.1899414688785228
1107	20-21	0.2727698712395821
1107	22-23	0.3271367367883897
1107	24-25	0.22556111706129656
1107	26-27	0.29851134042140615
1107	28-29	0.3478604812835684
1107	30-31	0.33610052494663023
1107	32-33	0.35190995872373065
1107	34-35	0.3089281351081965
1107	36-37	0.38266744858945856
1107	38-39	0.4290263868557673
1107	40-41	0.39364503913813564
1107	42-43	0.5499849025209684
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2314	100	-0.5036359991138859
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2316	3	-0.3044627365602892
2316	4	-0.2428967001410669
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2316	76-77	0.012695985173941438
2316	78-79	-0.28330585738890335
2316	80-81	0.013969958628472057
2316	82-83	0.012856265887830176
2316	84-85	0.0603485867838387
2316	86-87	0.11376265274093811
2316	88-89	-0.00980891428402586
2316	90-91	0.08229749219015403
2316	92-93	0.18721017261659867
2316	94-95	0.16807114305273885
2316	96-97	0.11242987693884743
2316	98-99	0.22164845164679292
2316	100	-0.012865274255332793
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	5.0
1	27.0
2	53.0
3	63.0
4	69.0
5	88.0
6	202.0
7	1586.0
8	2643.0
9	2970.0
10	3909.0
11	5500.0
12	7543.0
13	8872.0
14	10056.0
15	11271.0
16	13077.0
17	16190.0
18	20295.0
19	24195.0
20	28459.0
21	33474.0
22	38518.0
23	41185.0
24	40160.0
25	40507.0
26	43561.0
27	48487.0
28	55803.0
29	65884.0
30	79792.0
31	98160.0
32	123594.0
33	161839.0
34	225323.0
35	319158.0
36	483712.0
37	783963.0
38	855710.0
39	224417.0
40	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27160068336728	18.45101882990087	12.660434061367647	23.616946425364198
2	15.78758693027271	20.402318293803575	40.691499887271824	23.118594888651884
3	18.15381538000716	26.433397041852952	30.590677736691696	24.8221098414482
4	12.090407473537516	16.66846969960621	38.510997565395236	32.73012526146104
5	12.447886542743305	37.51324442739043	35.42674163741816	14.612127392448107
6	29.76196913521047	36.993697877663124	19.141408524852423	14.102924462273988
7	26.36904635888955	32.797273627663266	23.302132548199587	17.531547465247606
8	25.481204568631245	35.39939303257718	21.201824065151307	17.917578333640268
9	26.608015296187638	15.371714602441452	20.892860877847312	37.1274092235236
10-11	24.279054012346464	25.979039779373615	29.613192180333296	20.12871402794662
12-13	24.866151282625207	24.137063331273584	29.21739041712182	21.77939496897939
14-15	22.393760144389134	25.461187447584	27.096891870776822	25.048160537250038
16-17	21.77856177747259	28.066158838247475	27.84557921187199	22.309700172407947
18-19	21.426077817254644	27.228054466593584	29.780224195427703	21.56564352072408
20-21	22.374724106298284	26.60259419161487	29.75843293397837	21.264248768108473
22-23	22.068157912330925	26.43214141536835	29.54993413769891	21.94976653460182
24-25	21.703592444713372	26.500759161208492	29.729260562697636	22.0663878313805
26-27	21.58615889699103	26.65959151793316	29.635605182212714	22.1186444028631
28-29	21.59723338410395	27.02653848999513	29.578068114267055	21.79816001163387
30-31	22.2411575513486	26.66817259292441	29.63069773279443	21.459972122932562
32-33	21.87814904595547	26.717405158070306	29.59291940126097	21.81152639471325
34-35	22.02691103709168	26.938997405890007	29.523438639036808	21.510652917981503
36-37	21.999247154602834	26.974595996143165	29.30939892144884	21.716757927805162
38-39	21.86678789427993	27.21896661112952	29.19701502513202	21.717230469458524
40-41	21.994597453384028	26.880080354461754	29.39790536511964	21.72741682703457
42-43	21.800009134356433	27.096634602013793	29.020041747580986	22.083314516048784
44-45	21.71177775333954	27.293944621536614	29.1083178942926	21.885959730831242
46-47	21.75027548875257	26.99595890303497	28.80713720915193	22.446628399060526
48-49	21.768208144831007	27.141171713873558	28.833451738515443	22.257168402779993
50-51	21.836762076959495	27.39892586893703	28.81231327445186	21.95199877965161
52-53	21.865637296520646	27.540494330334546	28.458970439368457	22.134897933776347
54-55	21.75182243385266	27.389497607039047	28.680336695818294	22.178343263289996
56-57	21.82659604538511	27.51163557214565	28.382173313645637	22.279595068823603
58-59	21.781336307742116	27.48916774254696	28.617965059966323	22.111530889744603
60-61	22.167788305825887	27.54839066030502	28.529157877052597	21.754663156816495
62-63	21.93692333231383	27.587796141995806	28.629665767999406	21.845614757690953
64-65	21.89148221553635	27.257655300231466	28.531072293140543	22.31979019109164
66-67	21.77875706707617	27.356739078885557	28.420925344200075	22.443578509838197
68-69	21.612455269601902	27.457142244834543	28.63972646146514	22.290676024098413
70-71	21.761354741837565	27.295090155496204	28.609891033110323	22.33366406955591
72-73	21.634924334716164	27.64655623670067	28.23126426733651	22.487255161246658
74-75	21.757375130021398	27.60703346064681	28.21494608473727	22.420645324594517
76-77	21.707568237279666	27.37773504313298	28.338639518658688	22.57605720092867
78-79	21.66902103240479	27.38550607809011	28.3514316563234	22.5940412331817
80-81	21.69647515335819	27.62911873093785	28.062297419857668	22.612108695846285
82-83	21.942446134929263	27.555587018301114	28.130602707156672	22.371364139612957
84-85	21.880045913950504	27.73689352251813	27.940990956115037	22.442069607416336
86-87	21.64300359633769	27.72778399829992	28.122113229150003	22.50709917621238
88-89	21.6113343767941	27.440246012180964	28.24179582055315	22.706623790471784
90-91	22.139856305706225	27.363862470775942	28.109633329646638	22.3866478938712
92-93	21.941187058412773	27.473223327962458	28.06788913093478	22.517700482689992
94-95	21.782080089634757	27.375616978765827	28.076258422391547	22.76604450920787
96-97	21.787706290727233	27.255107285846027	28.16562439813935	22.791562025287394
98-99	21.78000224237869	27.208025521789914	28.09135435463436	22.920617881197042
100	21.88736307480005	27.240995496727678	28.03981088705891	22.831830541413368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2038.0
1	1469.0
2	2315.5
3	3398.5
4	2196.0
5	1523.0
6	2151.5
7	3876.5
8	6129.5
9	7185.0
10	6032.5
11	4522.5
12	4412.5
13	4738.0
14	5028.5
15	5299.0
16	5580.5
17	6015.0
18	6510.5
19	6935.5
20	7627.5
21	8745.5
22	10408.0
23	12726.5
24	15728.5
25	19529.5
26	24330.5
27	29627.0
28	35407.5
29	42226.0
30	49869.0
31	57360.0
32	65189.0
33	74189.5
34	83347.0
35	91224.0
36	100551.5
37	109530.5
38	115308.5
39	120511.0
40	122986.0
41	124731.5
42	126965.0
43	130026.5
44	133120.0
45	136343.0
46	139551.0
47	141306.0
48	145040.5
49	148895.5
50	149765.5
51	146134.5
52	138725.0
53	131025.0
54	123256.0
55	113243.5
56	102287.5
57	92059.5
58	81912.0
59	70078.0
60	56833.0
61	45402.0
62	36625.5
63	28827.5
64	21729.5
65	16212.0
66	12384.0
67	9541.5
68	7363.0
69	5818.0
70	4616.0
71	3684.5
72	2935.0
73	2301.5
74	1896.5
75	1559.0
76	1195.5
77	902.5
78	706.0
79	559.0
80	438.0
81	381.0
82	322.0
83	238.0
84	198.5
85	171.5
86	139.5
87	112.5
88	89.5
89	61.5
90	42.5
91	36.0
92	28.5
93	23.0
94	14.5
95	5.5
96	8.5
97	10.0
98	7.0
99	6.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16614545353449328
2	0.10610455736928034
3	0.0318313672107841
4	0.03415240440323711
5	0.027750422696581012
6	0.001963954547460237
7	0.0035708264499277036
8	0.0047696039009748606
9	0.005279721965250247
10-11	0.0047696039009748606
12-13	0.0072309235611035996
14-15	0.017815873394817865
16-17	0.007855818189840947
18-19	0.01641304871806055
20-21	0.017165472862866748
22-23	0.047925592138672535
24-25	0.013900717251504274
26-27	0.009347913527846453
28-29	0.02731682234194693
30-31	0.015125000605765201
32-33	0.020991358344932144
34-35	0.02090208768368395
36-37	0.02207535923151734
38-39	0.019435498248892215
40-41	0.026220068503754853
42-43	0.03550421727356688
44-45	0.037850760369233655
46-47	0.02064702865154626
48-49	0.01290598702616727
50-51	0.011962268607257806
52-53	0.02175653544134522
54-55	0.026526139342320086
56-57	0.02002213402280891
58-59	0.025569667971803735
60-61	0.026156303745720432
62-63	0.053065031636247054
64-65	0.032775085629693564
66-67	0.020698040457973795
68-69	0.027214798729091855
70-71	0.04148535157719578
72-73	0.03890925535260509
74-75	0.04066916267435517
76-77	0.02204985332830357
78-79	0.016285519201991706
80-81	0.02336340734381269
82-83	0.017369520088576902
84-85	0.01835149736230702
86-87	0.021424958699566222
88-89	0.017662837975535247
90-91	0.029574094776365516
92-93	0.027151033971057434
94-95	0.01920594511996829
96-97	0.013416105090442658
98-99	0.01827497965266571
100	0.04420173026946221
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3920661.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.05706456289882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.20345393690275	53.11151124251471
2	13.880822441337695	18.61614413269833
3	3.6394557605939557	7.321536597358888
4	1.270077920224982	3.406707883857555
5	0.6078474511148089	2.038023288689961
6	0.35284693967437836	1.4196528008739633
7	0.22018220878043454	1.0335340812853886
8	0.1524755785037645	0.8179651769593828
9	0.11030372086968727	0.665697935869793
>10	0.5157280592439947	5.999007517819968
>50	0.02821268553085237	1.2997264860835567
>100	0.01741633697755153	2.195563720112616
>500	6.790155295081414E-4	0.3115960463083306
>1k	3.168739099044592E-4	0.4316204716915341
>5k	9.053540282984549E-5	0.43477741163498435
>10k+	9.053540282984549E-5	0.8969352062410471
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15464	0.3944232872977287	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14084	0.359225140862727	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9018	0.23001223518177163	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5305	0.1353088165490462	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.550590321376931E-5	0.0
5	0.0	0.0	0.0	2.550590321376931E-5	0.0
6	0.0	0.0	0.0	5.101180642753862E-5	0.0
7	0.0	0.0	0.0	5.101180642753862E-5	0.0
8	0.0	0.0	0.0	5.101180642753862E-5	0.0
9	0.0	0.0	0.0	5.101180642753862E-5	0.0
10-11	0.0	0.0	0.0	1.0202361285507723E-4	0.0
12-13	0.0	0.0	0.0	1.2752951606884654E-4	0.0
14-15	0.0	0.0	0.0	1.2752951606884654E-4	3.8258854820653964E-5
16-17	0.0	0.0	0.0	1.2752951606884654E-4	5.101180642753862E-5
18-19	0.0	1.2752951606884655E-5	0.0	1.2752951606884654E-4	5.101180642753862E-5
20-21	0.0	2.550590321376931E-5	0.0	1.4028246767573119E-4	5.101180642753862E-5
22-23	0.0	2.550590321376931E-5	0.0	2.805649353514624E-4	7.651770964130793E-5
24-25	0.0	2.550590321376931E-5	0.0	4.7185920945473224E-4	1.0202361285507724E-4
26-27	0.0	2.550590321376931E-5	0.0	7.269182415924253E-4	1.0202361285507724E-4
28-29	0.0	2.550590321376931E-5	0.0	0.0017216484669294286	1.0202361285507724E-4
30-31	0.0	2.550590321376931E-5	0.0	0.005215957207215825	1.0202361285507724E-4
32-33	0.0	2.550590321376931E-5	0.0	0.013989987912752466	1.0202361285507724E-4
34-35	0.0	2.550590321376931E-5	0.0	0.027559128422477742	1.0202361285507724E-4
36-37	0.0	2.550590321376931E-5	0.0	0.04818065117081023	1.0202361285507724E-4
38-39	0.0	2.550590321376931E-5	0.0	0.08512595197595507	1.0202361285507724E-4
40-41	0.0	2.550590321376931E-5	0.0	0.12786109281062558	1.0202361285507724E-4
42-43	0.0	2.550590321376931E-5	0.0	0.16898936174282858	1.0202361285507724E-4
44-45	0.0	2.550590321376931E-5	0.0	0.2190829556546715	1.0202361285507724E-4
46-47	0.0	2.550590321376931E-5	0.0	0.2801568409000421	1.0202361285507724E-4
48-49	0.0	2.550590321376931E-5	0.0	0.3467527541911938	1.0202361285507724E-4
50-51	0.0	2.550590321376931E-5	0.0	0.42189314505895814	1.0202361285507724E-4
52-53	0.0	2.550590321376931E-5	0.0	0.5007573977959329	1.147765644619619E-4
54-55	0.0	2.550590321376931E-5	0.0	0.5910227892694625	1.6578837088950053E-4
56-57	0.0	5.101180642753862E-5	0.0	0.6928423548988296	1.7854132249638517E-4
58-59	0.0	5.101180642753862E-5	0.0	0.8126053234390833	1.7854132249638517E-4
60-61	0.0	5.101180642753862E-5	0.0	0.9425451473616311	2.0404722571015448E-4
62-63	0.0	5.101180642753862E-5	0.0	1.0739898195737911	2.295531289239238E-4
64-65	0.0	7.651770964130793E-5	0.0	1.2088267769133827	2.295531289239238E-4
66-67	0.0	7.651770964130793E-5	0.0	1.370776509369211	2.4230608053080844E-4
68-69	0.0	7.651770964130793E-5	0.0	1.5606169469892959	2.550590321376931E-4
70-71	0.0	7.651770964130793E-5	0.0	1.7700969300839833	2.550590321376931E-4
72-73	0.0	7.651770964130793E-5	0.0	1.97601883968035	3.3157674177900105E-4
74-75	0.0	7.651770964130793E-5	0.0	2.1880494130964143	3.3157674177900105E-4
76-77	0.0	7.651770964130793E-5	0.0	2.4206249915511693	3.3157674177900105E-4
78-79	0.0	7.651770964130793E-5	0.0	2.696802911549864	3.3157674177900105E-4
80-81	0.0	7.651770964130793E-5	0.0	2.9909242344594444	3.443296933858857E-4
82-83	0.0	7.651770964130793E-5	0.0	3.2895601022378624	3.5708264499277034E-4
84-85	0.0	7.651770964130793E-5	0.0	3.600489815365317	3.5708264499277034E-4
86-87	0.0	7.651770964130793E-5	0.0	3.921264807133287	3.5708264499277034E-4
88	0.0	7.651770964130793E-5	0.0	4.181820361413547	3.5708264499277034E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7935	0.0	48.018406	1
TACCTGG	3780	0.0	40.182762	2
GTACCTG	4135	0.0	37.587593	1
GTATCAA	13565	0.0	35.692703	1
ACCTGGG	4390	0.0	33.827423	3
TCAACGC	16340	0.0	28.931442	4
ATCAACG	16420	0.0	28.820576	3
CAACGCA	16390	0.0	28.7524	5
AACGCAG	17000	0.0	27.906683	6
TATCAAC	17060	0.0	27.785011	2
GTACATG	14170	0.0	27.321724	1
TATCACG	285	0.0	26.400017	2
TACATGG	14260	0.0	26.282572	2
ACGCAGA	18960	0.0	24.998636	7
ACATGGG	14335	0.0	24.915077	3
CGCAGAG	19465	0.0	24.231571	8
CATGGGG	8510	0.0	22.971426	4
ATGGGGG	5720	0.0	22.342154	5
GCAGAGT	21085	0.0	21.792461	9
GAGTACT	13405	0.0	21.451286	12-13
>>END_MODULE