FastQCFastQC Report
Mon 6 Jun 2016
ERR1211552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3920661
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170800.43564082689117983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157550.4018455051329355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108980.27796333322365796No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52370.13357441513050988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81600.046.803981
TACCTGG39800.041.3620382
GTATCAA136450.036.1936761
GTACCTG46800.035.3764081
ACCTGGG49600.032.68513
TCAACGC168350.028.5262494
TATAACG4650.028.3218752
ATCAACG170550.028.1310833
CAACGCA171500.028.0567385
TATCAAC174850.027.6531372
AACGCAG178900.027.1063296
GTACATG136650.027.0538981
TACATGG139950.025.8110262
ACATGGG138650.024.8086763
TAACGCA5400.024.3652654
CATGGGG81250.024.2324624
ACGCAGA200700.024.0680877
ATGGGGG52950.023.8719355
CGCAGAG203800.023.6097628
CCTGGGG43250.022.5987344
Produced by FastQC (version 0.11.5)