##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211552_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3920661 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1118387945298 33.0 31.0 34.0 30.0 34.0 2 32.327177993710755 34.0 31.0 34.0 30.0 34.0 3 32.33647821119959 34.0 31.0 34.0 30.0 34.0 4 35.978958395025735 37.0 35.0 37.0 35.0 37.0 5 35.907834673796074 37.0 35.0 37.0 35.0 37.0 6 35.92660089714464 37.0 35.0 37.0 35.0 37.0 7 35.85435542629164 37.0 35.0 37.0 35.0 37.0 8 35.84867322117368 37.0 35.0 37.0 35.0 37.0 9 37.50095277301455 39.0 37.0 39.0 35.0 39.0 10-11 37.46408883604066 39.0 37.0 39.0 34.5 39.0 12-13 37.37325274998272 39.0 37.0 39.0 34.0 39.0 14-15 38.59648832173963 40.0 38.0 41.0 34.0 41.0 16-17 38.52354284137292 40.0 38.0 41.0 34.0 41.0 18-19 38.5211410779968 40.0 38.0 41.0 34.0 41.0 20-21 38.46119264073074 40.0 38.0 41.0 34.0 41.0 22-23 38.34721568633452 40.0 38.0 41.0 34.0 41.0 24-25 38.233115921014345 40.0 38.0 41.0 33.5 41.0 26-27 38.053526305895865 40.0 37.0 41.0 33.0 41.0 28-29 38.07928867096645 40.0 37.5 41.0 33.0 41.0 30-31 37.98526128629841 40.0 37.0 41.0 33.0 41.0 32-33 37.77472765434196 40.0 37.0 41.0 33.0 41.0 34-35 37.64291225382659 40.0 37.0 41.0 33.0 41.0 36-37 37.57364064375879 40.0 37.0 41.0 32.5 41.0 38-39 37.47021420622696 40.0 36.5 41.0 32.0 41.0 40-41 37.29827980536955 40.0 36.0 41.0 31.0 41.0 42-43 37.13824314318427 40.0 36.0 41.0 31.0 41.0 44-45 36.94838115307597 39.0 36.0 41.0 30.5 41.0 46-47 36.67333595023901 39.0 35.0 41.0 30.0 41.0 48-49 36.435949703379094 39.0 35.0 41.0 29.5 41.0 50-51 36.22693724349032 39.0 35.0 40.5 28.5 41.0 52-53 36.032722670998595 39.0 35.0 40.0 28.0 41.0 54-55 35.838311575522596 38.5 35.0 40.0 28.0 41.0 56-57 35.64087152651045 38.0 34.0 40.0 27.0 41.0 58-59 35.38725549084708 38.0 34.0 40.0 26.5 41.0 60-61 35.07295848837734 38.0 34.0 40.0 26.0 41.0 62-63 34.7417583412593 37.0 33.5 40.0 26.0 41.0 64-65 34.43147163195186 37.0 33.0 40.0 25.5 41.0 66-67 34.09401361148031 36.0 33.0 39.0 25.0 41.0 68-69 33.70103727407189 36.0 32.0 39.0 24.5 40.0 70-71 33.3131784410843 35.0 32.0 38.5 23.5 40.0 72-73 32.904077781782206 35.0 31.0 38.0 22.5 40.0 74-75 32.49724587257097 35.0 31.0 37.0 22.0 39.0 76-77 30.944321123402407 33.5 29.5 35.5 20.5 38.0 78-79 31.78388019265119 34.0 30.5 36.0 21.5 39.0 80-81 31.75835898589549 34.0 31.0 36.0 21.5 37.5 82-83 31.476279510011196 34.0 31.0 35.5 20.5 37.0 84-85 31.17290107458921 34.0 30.0 35.0 20.0 37.0 86-87 30.877022522477716 34.0 30.0 35.0 20.0 36.0 88-89 30.60373633425588 34.0 30.0 35.0 19.0 36.0 90-91 30.313748498021127 34.0 29.0 35.0 18.5 35.5 92-93 30.101414531886334 34.0 29.0 35.0 18.0 35.0 94-95 29.880236521341683 34.0 29.0 35.0 18.0 35.0 96-97 29.67304314247011 34.0 29.0 35.0 17.0 35.0 98-99 29.510993809462228 33.5 29.0 35.0 16.5 35.0 100 27.65675787832715 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2488382328064489 1101 2 0.24390145562938415 1101 3 0.31238503071764256 1101 4 0.16337446032981262 1101 5 0.18469312597349585 1101 6 0.1777001328392629 1101 7 0.2371957718605131 1101 8 0.20570215077175646 1101 9 0.16479123278580943 1101 10-11 0.22024843931261984 1101 12-13 0.21558287329626324 1101 14-15 0.33769109568473965 1101 16-17 0.35506775367606735 1101 18-19 0.36112187821407815 1101 20-21 0.3843054180286458 1101 22-23 0.34113502068260715 1101 24-25 0.35305097502855887 1101 26-27 0.36900163176870393 1101 28-29 0.4270718574995769 1101 30-31 0.44233977729867746 1101 32-33 0.43996964787557147 1101 34-35 0.3735679395294724 1101 36-37 0.4099417022621452 1101 38-39 0.4506133848348739 1101 40-41 0.45398038893146264 1101 42-43 0.5145448130158243 1101 44-45 0.4178208120372773 1101 46-47 0.4609123583077448 1101 48-49 0.45645383175471466 1101 50-51 0.44736031674283794 1101 52-53 0.33610640048788554 1101 54-55 0.29346927064250394 1101 56-57 0.2853032320624962 1101 58-59 0.41305923071766415 1101 60-61 0.35307052772056835 1101 62-63 0.2869106648390414 1101 64-65 0.2375168605899205 1101 66-67 0.17741401101140752 1101 68-69 0.21945709730844953 1101 70-71 0.25207322540064325 1101 72-73 0.17347959566272664 1101 74-75 0.0711777904514932 1101 76-77 0.21498643374926374 1101 78-79 0.31863277705195614 1101 80-81 0.2801625713302194 1101 82-83 0.23032462185912195 1101 84-85 0.1781539465027393 1101 86-87 0.1102034909476366 1101 88-89 0.04502067659797149 1101 90-91 0.13757348610441866 1101 92-93 0.0015709134652226453 1101 94-95 -0.08815049682931075 1101 96-97 -0.09107099964800014 1101 98-99 -0.0650249971187975 1101 100 0.14751459147460722 1102 1 0.33049941330877175 1102 2 0.2690564152028827 1102 3 0.34144632429622845 1102 4 0.19085754224767015 1102 5 0.2018106524475627 1102 6 0.178497910162271 1102 7 0.15558384543639647 1102 8 0.12550541194671183 1102 9 0.26530146341282546 1102 10-11 0.20511876581165467 1102 12-13 0.23151344585757982 1102 14-15 0.29074467510346125 1102 16-17 0.33947190073071454 1102 18-19 0.24716181564058815 1102 20-21 0.3295414734013278 1102 22-23 0.18875088049578892 1102 24-25 0.29409974003457506 1102 26-27 0.18833004200661208 1102 28-29 0.272942319273902 1102 30-31 0.44105604952805066 1102 32-33 0.4709771712625894 1102 34-35 0.38611073334565305 1102 36-37 0.501882417001255 1102 38-39 0.4866219314580249 1102 40-41 0.4001661461782291 1102 42-43 0.3030020157310389 1102 44-45 0.41361895356297396 1102 46-47 0.48438608888793766 1102 48-49 0.49169512470895427 1102 50-51 0.41299068222890867 1102 52-53 0.46328840818123496 1102 54-55 0.456852332095643 1102 56-57 0.47986794047488956 1102 58-59 0.47528655563155553 1102 60-61 0.5451742665213359 1102 62-63 0.4890667447693957 1102 64-65 0.44612973394104216 1102 66-67 0.43714776150134327 1102 68-69 0.5083079857895854 1102 70-71 0.48173632056853677 1102 72-73 0.35205441850413877 1102 74-75 0.33725595181756063 1102 76-77 0.3992428185692951 1102 78-79 0.43261746666454926 1102 80-81 0.41711249519451954 1102 82-83 0.3231557254626445 1102 84-85 0.27833153867203464 1102 86-87 0.26384950674362884 1102 88-89 0.18902538001558256 1102 90-91 0.18709464628075878 1102 92-93 0.09511758864151432 1102 94-95 0.2868967628055863 1102 96-97 0.28685033950795 1102 98-99 0.18386453153354765 1102 100 0.24266445221043753 1103 1 0.28036771066258126 1103 2 0.2637621253955089 1103 3 0.30337457949813995 1103 4 0.17805315839017766 1103 5 0.14871642177318023 1103 6 0.16082886718546519 1103 7 0.18190871773482087 1103 8 0.17289832582975606 1103 9 0.2330782533843916 1103 10-11 0.23912272557449654 1103 12-13 0.24456077945678345 1103 14-15 0.3002330474748618 1103 16-17 0.3204017220006037 1103 18-19 0.3205987654935498 1103 20-21 0.21324533559418057 1103 22-23 0.2332089999734137 1103 24-25 0.2539344540552335 1103 26-27 0.284956762459764 1103 28-29 0.24600319265217507 1103 30-31 0.37198698495215154 1103 32-33 0.4107124899029486 1103 34-35 0.3175905700667556 1103 36-37 0.34460274446961137 1103 38-39 0.3183931956521988 1103 40-41 0.35901436615255733 1103 42-43 0.272855247154979 1103 44-45 0.36037841333337184 1103 46-47 0.4114184197193893 1103 48-49 0.43376452962289136 1103 50-51 0.4857451124346852 1103 52-53 0.32936119413906084 1103 54-55 0.42275285431601617 1103 56-57 0.39566136762766746 1103 58-59 0.4695557952445526 1103 60-61 0.51769163570453 1103 62-63 0.49836186348613154 1103 64-65 0.3783404237617347 1103 66-67 0.3594134360346146 1103 68-69 0.37522036843535034 1103 70-71 0.42666880789991524 1103 72-73 0.35198855782962823 1103 74-75 0.40613368645831116 1103 76-77 0.41120365142655046 1103 78-79 0.421290579537132 1103 80-81 0.4086172697327193 1103 82-83 0.36372272147899665 1103 84-85 0.3046259253600674 1103 86-87 0.288599200110756 1103 88-89 0.3180317730164042 1103 90-91 0.3063591367112437 1103 92-93 0.24163135202625696 1103 94-95 0.31427610057263067 1103 96-97 0.2930233683514025 1103 98-99 0.367969457179921 1103 100 0.45371486344021505 1104 1 0.1947842144764067 1104 2 0.1275643857757629 1104 3 0.1861191074601649 1104 4 0.0016602802075524892 1104 5 0.10918647777309332 1104 6 0.1627919429574476 1104 7 0.14097500354706938 1104 8 0.15427970791070322 1104 9 0.1242571561490351 1104 10-11 0.1921960658163826 1104 12-13 0.2150187074625478 1104 14-15 0.2883449977502366 1104 16-17 0.2892088673041613 1104 18-19 0.2507212255677089 1104 20-21 0.13980148134548642 1104 22-23 0.24368152583375746 1104 24-25 0.2338671160771426 1104 26-27 0.30510057875255114 1104 28-29 0.3415468179318353 1104 30-31 0.23339927814370043 1104 32-33 0.26002979983368846 1104 34-35 0.3755501731037967 1104 36-37 0.24489029657956962 1104 38-39 0.32856613512018384 1104 40-41 0.44568785701091684 1104 42-43 0.5036731013285944 1104 44-45 0.40616205451332377 1104 46-47 0.44997561461869395 1104 48-49 0.40030565078704683 1104 50-51 0.40692074799488864 1104 52-53 0.44348153177454464 1104 54-55 0.3272036963372287 1104 56-57 0.5101236472468855 1104 58-59 0.5639703792911988 1104 60-61 0.44905818538682496 1104 62-63 0.4210811750468224 1104 64-65 0.5844851664771937 1104 66-67 0.5363896036551736 1104 68-69 0.4452984356141769 1104 70-71 0.3382192941500435 1104 72-73 0.5844100476377889 1104 74-75 0.5738133841579796 1104 76-77 0.45415230051349553 1104 78-79 0.4522771625035027 1104 80-81 0.6412721653216842 1104 82-83 0.5176367725599462 1104 84-85 0.45539393547373663 1104 86-87 0.5884871378048366 1104 88-89 0.554035301660722 1104 90-91 0.4361223207758833 1104 92-93 0.43054758733211074 1104 94-95 0.4760462667447314 1104 96-97 0.4258188950926787 1104 98-99 0.45631641745413276 1104 100 0.4623499508015314 1105 1 0.23638995700886767 1105 2 0.160963555666072 1105 3 0.2043711358875413 1105 4 0.1245327939013876 1105 5 0.10514786878260196 1105 6 0.1923754182692221 1105 7 0.17305345913171521 1105 8 0.18877164068636176 1105 9 0.23099457813635382 1105 10-11 0.19953195941462099 1105 12-13 0.2189745809116772 1105 14-15 0.2472988454110734 1105 16-17 0.30599362591705415 1105 18-19 0.27906342662569017 1105 20-21 0.24279051175943067 1105 22-23 0.36054874670351467 1105 24-25 0.35835120451609015 1105 26-27 0.2752290026388806 1105 28-29 0.3211405039025479 1105 30-31 0.3586795086200567 1105 32-33 0.45071807540905695 1105 34-35 0.43955026719940093 1105 36-37 0.4862651297889471 1105 38-39 0.44687104677668543 1105 40-41 0.40358012836999535 1105 42-43 0.4506917577109064 1105 44-45 0.5115146475089531 1105 46-47 0.48226640153925615 1105 48-49 0.5856061493276243 1105 50-51 0.675087321432251 1105 52-53 0.7359037653222984 1105 54-55 0.5847868454223359 1105 56-57 0.5535507237709965 1105 58-59 0.5939919135735892 1105 60-61 0.6717951231783204 1105 62-63 0.6556513850756005 1105 64-65 0.6841314452452778 1105 66-67 0.6939720346986675 1105 68-69 0.6601924566906305 1105 70-71 0.7623260731541421 1105 72-73 0.5667174218555147 1105 74-75 0.6626736061582292 1105 76-77 0.6368397973888094 1105 78-79 0.7774260985346153 1105 80-81 0.8019091400387488 1105 82-83 0.737911443840229 1105 84-85 0.7257664238485617 1105 86-87 0.657325144454866 1105 88-89 0.7155995681804086 1105 90-91 0.6393510859945479 1105 92-93 0.620852782347125 1105 94-95 0.7947868153210216 1105 96-97 0.8444937964463541 1105 98-99 0.6613107076836577 1105 100 0.9399499727928955 1106 1 0.19838805169224116 1106 2 0.20211697610755408 1106 3 0.20585802853211987 1106 4 0.13600489185692055 1106 5 0.10979117072140099 1106 6 0.15757979878176087 1106 7 0.13568592614941366 1106 8 0.17424926542388874 1106 9 0.14886381736907595 1106 10-11 0.12259426977930588 1106 12-13 0.19827680873756748 1106 14-15 0.23884809967163534 1106 16-17 0.28733790466606735 1106 18-19 0.2876181322210485 1106 20-21 0.3102798699655551 1106 22-23 0.3157710288777835 1106 24-25 0.27816111937733723 1106 26-27 0.22758426390394249 1106 28-29 0.39450184801522425 1106 30-31 0.3832617940145795 1106 32-33 0.35084930513674806 1106 34-35 0.40632210168365646 1106 36-37 0.35470490818975975 1106 38-39 0.4148679083493434 1106 40-41 0.4062728563601965 1106 42-43 0.3853594634078874 1106 44-45 0.537681791421285 1106 46-47 0.485343514291678 1106 48-49 0.3770933906130338 1106 50-51 0.5241980453601869 1106 52-53 0.5325583146121957 1106 54-55 0.4985645590687753 1106 56-57 0.4841865962507157 1106 58-59 0.5471383805552179 1106 60-61 0.6064879596101278 1106 62-63 0.5387008089273877 1106 64-65 0.5929763035959184 1106 66-67 0.6708137601210282 1106 68-69 0.5853589299367883 1106 70-71 0.5220358577095041 1106 72-73 0.45511411066949137 1106 74-75 0.5329158411460995 1106 76-77 0.4811485163130307 1106 78-79 0.5187521181825652 1106 80-81 0.5782638021254947 1106 82-83 0.6209297429744929 1106 84-85 0.5159483791671065 1106 86-87 0.5163424920306348 1106 88-89 0.5066033315216956 1106 90-91 0.5142866919235125 1106 92-93 0.4918162749231634 1106 94-95 0.7114459299360938 1106 96-97 0.592469012592673 1106 98-99 0.702733527402696 1106 100 0.8211044182604113 1107 1 0.15737365855641627 1107 2 0.1997282677697214 1107 3 0.1734903112991617 1107 4 0.16770063502499966 1107 5 0.13195070311844148 1107 6 0.09776239819957766 1107 7 0.17392388213499999 1107 8 0.14755095300880328 1107 9 0.17343090370915348 1107 10-11 0.14949414096010827 1107 12-13 0.1738735341965807 1107 14-15 0.16723368476857559 1107 16-17 0.15429612808529214 1107 18-19 0.06809855687833277 1107 20-21 0.1077986174397978 1107 22-23 0.2508872270880502 1107 24-25 0.2835898803133645 1107 26-27 0.2617653967099258 1107 28-29 0.26971729008182166 1107 30-31 0.14938231355557008 1107 32-33 0.2138210139803931 1107 34-35 0.28486128403863376 1107 36-37 0.30880838818130485 1107 38-39 0.3795305028784455 1107 40-41 0.3432227096544125 1107 42-43 0.3314149221210769 1107 44-45 0.415713369072769 1107 46-47 0.44029928399079665 1107 48-49 0.3816124886824426 1107 50-51 0.41945606734330454 1107 52-53 0.46121716098460297 1107 54-55 0.4257743568208596 1107 56-57 0.5741722423672684 1107 58-59 0.5995196844659105 1107 60-61 0.5327603278938327 1107 62-63 0.5748942955293899 1107 64-65 0.629296635617564 1107 66-67 0.640262580809889 1107 68-69 0.6182494733236155 1107 70-71 0.6335422371141419 1107 72-73 0.45239460062846604 1107 74-75 0.4968154116893331 1107 76-77 0.562331703624519 1107 78-79 0.6948588904678807 1107 80-81 0.6649938420647921 1107 82-83 0.630723993570637 1107 84-85 0.4982387513600024 1107 86-87 0.41528544314981986 1107 88-89 0.496434677851731 1107 90-91 0.3646148953864632 1107 92-93 0.5160106308762806 1107 94-95 0.776700566401022 1107 96-97 0.7504379492366446 1107 98-99 0.6236397489949006 1107 100 0.7350481365852986 1108 1 0.2738841916980519 1108 2 0.20873444875949332 1108 3 0.26502905043804503 1108 4 0.1803293431940176 1108 5 0.11728358455066257 1108 6 0.1535177568640762 1108 7 0.16903183439490732 1108 8 0.16712035781133494 1108 9 0.1440013535769964 1108 10-11 0.17496492963768873 1108 12-13 0.23948170236206323 1108 14-15 0.27827060784127156 1108 16-17 0.3442744309207484 1108 18-19 0.31173926424642673 1108 20-21 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2316 90-91 0.2272885497303676 2316 92-93 0.2291898440747353 2316 94-95 0.2124419143760896 2316 96-97 0.4016989004964664 2316 98-99 0.17994981657882292 2316 100 -0.08877712620720501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 6.0 11 17.0 12 47.0 13 138.0 14 447.0 15 1235.0 16 2868.0 17 6138.0 18 11107.0 19 16280.0 20 21926.0 21 28728.0 22 36128.0 23 40658.0 24 42483.0 25 45027.0 26 50975.0 27 60611.0 28 73099.0 29 87559.0 30 105316.0 31 127658.0 32 155236.0 33 195424.0 34 259048.0 35 352206.0 36 507801.0 37 785720.0 38 782707.0 39 124046.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89021065333986 19.86773683164746 13.750533411792373 23.491519103220316 2 15.279258492610323 21.365887648307222 40.15712238368643 23.197731475396026 3 17.277078146034544 27.351316139175452 31.087650619772212 24.283955095017795 4 11.921015936432811 17.725095322471667 38.210659027775826 32.14322971331969 5 12.108697786943052 38.14311348149962 35.45671001467889 14.291478716878434 6 29.76765789063574 37.826161589393166 18.76818101937923 13.637999500591869 7 26.30071816971679 33.139692516134396 23.190553837732974 17.36903547641584 8 25.65822010905953 35.996608691859514 20.825350946574396 17.51982025250656 9 26.63539471298065 15.427049381239225 20.800431764149856 37.137124141630274 10-11 24.347106266009735 26.453702577192978 29.40079491697956 19.798396239817727 12-13 24.931421002733977 24.47546472393303 29.172032471055264 21.421081802277726 14-15 22.366802434589474 25.81923048179886 27.075396215076996 24.73857086853467 16-17 21.74935338860589 28.467072205077166 27.788814123439042 21.9947602828779 18-19 21.444994654549205 27.458413183515322 29.904631240413675 21.191960921521794 20-21 22.32305177931824 26.84904410306508 29.844581061373677 20.983323056242998 22-23 22.102841842230177 26.658323685725442 29.674434999608483 21.5643994724359 24-25 21.722230001522703 26.756572424904885 29.874222739481937 21.646974834090475 26-27 21.749040051613843 26.802075215267045 29.761160990125752 21.687723742993356 28-29 21.692144766825265 27.166378192031775 29.737921686916003 21.40355535422696 30-31 22.292543046636325 26.772862079233995 29.83132056871361 21.10327430541607 32-33 21.948878496901703 26.88411449233295 29.77532188151536 21.39168512924999 34-35 22.067620244624568 27.109250802991202 29.672088810157383 21.151040142226847 36-37 22.084230727243604 27.113432800797426 29.463321032532676 21.339015439426294 38-39 21.987049404783768 27.296630202785703 29.366037067871382 21.35028332455915 40-41 22.11389525474912 27.036741198777538 29.467529819072396 21.38183372740095 42-43 21.88451278472353 27.251132670234686 29.187130233737424 21.677224311304357 44-45 21.80107431572251 27.448526941684488 29.184195483731585 21.56620325886142 46-47 21.83849202219379 27.1715646662463 28.90169208259616 22.088251228963752 48-49 21.835429274870677 27.337500601035963 28.91909564908632 21.907974475007045 50-51 21.912696835656302 27.60231959532071 28.807891358374878 21.677092210648112 52-53 21.947897697193355 27.645157879795136 28.4847067279837 21.922237695027814 54-55 21.86237946747801 27.518762894328773 28.72070531571872 21.8981523224745 56-57 21.967722534081798 27.64471882645576 28.369409738588896 22.01814890087355 58-59 21.895449176300325 27.60631626792513 28.623216222854847 21.875018332919698 60-61 22.222086180641114 27.70739751605078 28.539321543629807 21.531194759678296 62-63 21.99364150171373 27.745141205293322 28.616252409372393 21.644964883620553 64-65 21.932612533610136 27.4032959771907 28.570249787878172 22.09384170132099 66-67 21.829925246000997 27.466874952251235 28.47789246550961 22.22530733623816 68-69 21.667443206218508 27.614737066853866 28.60684978034751 22.110969946580116 70-71 21.79922702317196 27.40949457559226 28.58337150943723 22.207906891798547 72-73 21.671705522505224 27.786795498126132 28.20516188852193 22.336337090846715 74-75 21.767433532205434 27.783130658599042 28.100919608629095 22.34851620056643 76-77 21.569607706476635 27.556107292695035 28.332210828260806 22.54207417256752 78-79 21.556500707333363 27.54920596537357 28.349987424617858 22.544305902675216 80-81 21.553938219673867 27.72665255090055 28.090196021414947 22.629213208010633 82-83 21.7782641720254 27.764459333890727 28.02553059596263 22.43174589812124 84-85 21.66413071165458 27.834658369295678 27.96250417574787 22.53870674330187 86-87 21.41883147281652 27.848769410802472 28.040846076153592 22.69155304022742 88-89 21.36303156801235 27.57808325487495 28.175702040394086 22.88318313671861 90-91 21.78714032087586 27.532681635294864 28.04928886302529 22.630889180803983 92-93 21.554657349683726 27.657987936349258 27.97166683952941 22.815687874437607 94-95 21.35023675550234 27.48619431238203 28.027245860207405 23.136323071908222 96-97 21.232395745091754 27.445079163738654 28.123348509875168 23.19917658129442 98-99 21.17421049731424 27.359183925451845 28.014526257571553 23.45207931966236 100 20.36967875051276 27.92269481400755 28.191168037094407 23.516458398385286 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2067.0 1 1581.0 2 2473.5 3 3415.0 4 2189.5 5 1623.5 6 2329.5 7 4156.0 8 6397.0 9 7409.5 10 6320.5 11 4884.5 12 4886.5 13 5346.0 14 5692.5 15 6020.5 16 6386.5 17 6730.5 18 7102.5 19 7603.5 20 8529.0 21 9666.0 22 11095.5 23 13546.5 24 16751.0 25 20376.5 26 25110.0 27 30524.0 28 36194.5 29 42902.0 30 50622.0 31 58316.5 32 66678.5 33 75817.5 34 84825.5 35 92352.0 36 101364.0 37 110294.5 38 115923.0 39 121165.5 40 123688.0 41 125113.5 42 127272.5 43 129984.0 44 132826.0 45 136056.0 46 139117.0 47 140832.0 48 144527.5 49 147690.5 50 148718.0 51 145948.0 52 138423.0 53 130536.0 54 122809.0 55 112439.0 56 101536.5 57 91372.5 58 80056.5 59 68093.0 60 55172.0 61 43971.0 62 35660.5 63 27732.5 64 20665.5 65 15502.5 66 11574.5 67 8770.5 68 6710.0 69 5160.5 70 4004.5 71 3053.5 72 2353.0 73 1932.0 74 1587.0 75 1211.0 76 882.0 77 594.5 78 410.5 79 286.0 80 197.5 81 142.0 82 105.0 83 88.0 84 68.5 85 47.0 86 31.0 87 22.0 88 12.5 89 8.0 90 7.0 91 5.0 92 3.0 93 2.0 94 2.0 95 1.5 96 3.0 97 3.0 98 1.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003672850062782781 2 0.06876391506432207 3 6.886593867717714E-4 4 5.611298707029249E-4 5 0.0016833896121087745 6 5.101180642753862E-4 7 0.0 8 0.0012752951606884654 9 5.1011806427538614E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.524241448061946E-4 18-19 9.564713705163491E-4 20-21 0.0032775085629693566 22-23 0.0 24-25 0.0 26-27 1.9129427410326984E-4 28-29 9.94730225337003E-4 30-31 0.0014920953380055046 32-33 0.003035202482438548 34-35 0.004170215175451282 36-37 0.0012497892574746961 38-39 0.0017599073217500823 40-41 0.005751581174704979 42-43 0.0071161469966416376 44-45 0.005407251481319094 46-47 0.007269182415924253 48-49 0.008837795463571066 50-51 0.008212900834833718 52-53 0.004144709272237513 54-55 0.0023082842408461224 56-57 0.002372048998880546 58-59 0.0030096965792247785 60-61 0.007422217835206869 62-63 0.014104764477214428 64-65 0.0039406620465273585 66-67 0.010266126043542148 68-69 0.012536151429567616 70-71 0.0059428754488082495 72-73 0.007485982593241292 74-75 0.0034305439822519723 76-77 0.002920425917976586 78-79 0.0077282886737721004 80-81 0.005662310513456787 82-83 0.01679563726626709 84-85 0.01796890881410048 86-87 0.02224114760240684 88-89 0.021794794296165876 90-91 0.024281619859508385 92-93 0.034853816741615765 94-95 0.03911330257831524 96-97 0.02309559536006811 98-99 0.01849177982998275 100 0.018287732604272595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3920661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.07504182062252 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33029504052087 51.756775206241166 2 14.434226268556674 19.074842086864205 3 3.8224392588259244 7.577035016511369 4 1.3318235726732746 3.5200119304830997 5 0.6462154383755604 2.1349356057898534 6 0.35781437648823333 1.4185559934287992 7 0.22727929248186177 1.0512242128990357 8 0.15517866812730396 0.8202749588944088 9 0.10926306594932864 0.6497605486846193 >10 0.5356033845461761 6.050835199915417 >50 0.030585696002563362 1.3938919769953027 >100 0.017898624182900467 2.2144280119055506 >500 8.263879637923135E-4 0.3680890640738263 >1k 3.672835371763618E-4 0.4838183990428021 >5k 4.591044214704522E-5 0.15886619573560437 >10k+ 1.3773132644113567E-4 1.3266555925348837 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17080 0.43564082689117983 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15755 0.4018455051329355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10898 0.27796333322365796 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5237 0.13357441513050988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.550590321376931E-5 2 0.0 0.0 0.0 0.0 2.550590321376931E-5 3 0.0 0.0 0.0 0.0 2.550590321376931E-5 4 0.0 0.0 0.0 0.0 2.550590321376931E-5 5 0.0 0.0 0.0 0.0 2.550590321376931E-5 6 0.0 0.0 0.0 0.0 2.550590321376931E-5 7 0.0 0.0 0.0 0.0 2.550590321376931E-5 8 0.0 0.0 0.0 0.0 2.550590321376931E-5 9 0.0 0.0 0.0 0.0 2.550590321376931E-5 10-11 0.0 0.0 0.0 2.550590321376931E-5 2.550590321376931E-5 12-13 0.0 0.0 0.0 5.101180642753862E-5 2.550590321376931E-5 14-15 0.0 0.0 0.0 5.101180642753862E-5 2.550590321376931E-5 16-17 0.0 0.0 0.0 5.101180642753862E-5 2.550590321376931E-5 18-19 0.0 0.0 0.0 5.101180642753862E-5 2.550590321376931E-5 20-21 0.0 0.0 0.0 6.376475803442327E-5 2.550590321376931E-5 22-23 0.0 0.0 0.0 2.0404722571015448E-4 7.651770964130793E-5 24-25 0.0 0.0 0.0 3.953414998134243E-4 1.2752951606884654E-4 26-27 0.0 0.0 0.0 6.504005319511175E-4 1.5303541928261586E-4 28-29 0.0 2.550590321376931E-5 0.0 0.0016706366605018898 1.5303541928261586E-4 30-31 0.0 2.550590321376931E-5 0.0 0.005228710158822709 1.5303541928261586E-4 32-33 0.0 2.550590321376931E-5 0.0 0.013824199541862967 1.5303541928261586E-4 34-35 0.0 2.550590321376931E-5 0.0 0.027482610712836433 2.0404722571015448E-4 36-37 0.0 2.550590321376931E-5 0.0 0.048116886412775804 2.0404722571015448E-4 38-39 0.0 3.8258854820653964E-5 0.0 0.0850239283631 2.295531289239238E-4 40-41 1.2752951606884655E-5 5.101180642753862E-5 0.0 0.12763153968170163 2.295531289239238E-4 42-43 2.550590321376931E-5 5.101180642753862E-5 0.0 0.1686450320494427 2.4230608053080844E-4 44-45 2.550590321376931E-5 5.101180642753862E-5 0.0 0.21848356692914792 2.550590321376931E-4 46-47 2.550590321376931E-5 5.101180642753862E-5 0.0 0.2789963223038156 2.550590321376931E-4 48-49 2.550590321376931E-5 5.101180642753862E-5 0.0 0.3451586352403332 2.550590321376931E-4 50-51 2.550590321376931E-5 5.101180642753862E-5 0.0 0.42000570822113925 2.550590321376931E-4 52-53 2.550590321376931E-5 5.101180642753862E-5 0.0 0.49881894915168645 2.6781198374457773E-4 54-55 2.550590321376931E-5 7.651770964130793E-5 0.0 0.5885487166577268 3.0607083856523177E-4 56-57 2.550590321376931E-5 7.651770964130793E-5 0.0 0.6898454112712117 3.3157674177900105E-4 58-59 2.550590321376931E-5 7.651770964130793E-5 0.0 0.8087411791021972 3.3157674177900105E-4 60-61 2.550590321376931E-5 7.651770964130793E-5 0.0 0.936908342751388 3.3157674177900105E-4 62-63 2.550590321376931E-5 1.0202361285507723E-4 0.0 1.066822660770722 4.0809445142030897E-4 64-65 2.550590321376931E-5 1.2752951606884654E-4 0.0 1.1999889814498117 4.0809445142030897E-4 66-67 2.550590321376931E-5 1.2752951606884654E-4 0.0 1.3597834650840763 4.0809445142030897E-4 68-69 2.550590321376931E-5 1.2752951606884654E-4 0.0 1.5479150071888388 4.0809445142030897E-4 70-71 2.550590321376931E-5 1.2752951606884654E-4 0.0 1.7539134344948466 4.0809445142030897E-4 72-73 2.550590321376931E-5 1.2752951606884654E-4 0.0 1.9570169417860916 4.336003546340783E-4 74-75 2.550590321376931E-5 1.2752951606884654E-4 0.0 2.1661015833809656 4.336003546340783E-4 76-77 2.550590321376931E-5 1.2752951606884654E-4 0.0 2.3955526886920344 4.336003546340783E-4 78-79 2.550590321376931E-5 1.5303541928261586E-4 0.0 2.6682745588052628 4.336003546340783E-4 80-81 2.550590321376931E-5 1.5303541928261586E-4 0.0 2.959118373151874 4.4635330624096295E-4 82-83 2.550590321376931E-5 1.5303541928261586E-4 0.0 3.254234426286792 4.591062578478476E-4 84-85 2.550590321376931E-5 1.5303541928261586E-4 0.0 3.5604200414164855 4.591062578478476E-4 86-87 2.550590321376931E-5 1.5303541928261586E-4 0.0 3.8756985110418882 4.591062578478476E-4 88 2.550590321376931E-5 1.5303541928261586E-4 0.0 4.130374954631375 4.591062578478476E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8160 0.0 46.80398 1 TACCTGG 3980 0.0 41.362038 2 GTATCAA 13645 0.0 36.193676 1 GTACCTG 4680 0.0 35.376408 1 ACCTGGG 4960 0.0 32.6851 3 TCAACGC 16835 0.0 28.526249 4 TATAACG 465 0.0 28.321875 2 ATCAACG 17055 0.0 28.131083 3 CAACGCA 17150 0.0 28.056738 5 TATCAAC 17485 0.0 27.653137 2 AACGCAG 17890 0.0 27.106329 6 GTACATG 13665 0.0 27.053898 1 TACATGG 13995 0.0 25.811026 2 ACATGGG 13865 0.0 24.808676 3 TAACGCA 540 0.0 24.365265 4 CATGGGG 8125 0.0 24.232462 4 ACGCAGA 20070 0.0 24.068087 7 ATGGGGG 5295 0.0 23.871935 5 CGCAGAG 20380 0.0 23.609762 8 CCTGGGG 4325 0.0 22.598734 4 >>END_MODULE