FastQCFastQC Report
Mon 6 Jun 2016
ERR1211551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4301365
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157460.3660698406203612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136190.3166204216568461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89630.20837571329101343No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.1260297603202704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79400.048.444831
TACCTGG41300.039.0529562
GTATCAA132000.036.80881
GTACCTG48050.034.4901581
ACCTGGG53400.030.0944583
TCAACGC159500.029.9613884
ATCAACG160050.029.9465073
CAACGCA161950.029.4487295
TATCAAC167200.028.6300012
AACGCAG166700.028.5780986
ATAACGC3750.027.5672283
GTACATG145100.027.0934891
TACATGG146950.026.2394142
TAACGCA4050.025.525214
ACGCAGA186400.025.3312247
ACATGGG147950.024.8684353
CGCAGAG191600.024.42398
CATGGGG94400.023.1463554
ATGGGGG60900.021.9120255
GCAGAGT210450.021.6123949
Produced by FastQC (version 0.11.5)