##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211551_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4301365
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.367106255804842	31.0	31.0	34.0	28.0	34.0
2	31.39424206036921	31.0	31.0	34.0	28.0	34.0
3	31.491947323698408	33.0	31.0	34.0	28.0	34.0
4	35.03990919161708	37.0	35.0	37.0	32.0	37.0
5	35.1266023692479	37.0	35.0	37.0	32.0	37.0
6	35.242673895379724	37.0	35.0	37.0	32.0	37.0
7	35.17327313538842	37.0	35.0	37.0	32.0	37.0
8	35.14176011568421	37.0	35.0	37.0	32.0	37.0
9	36.715817420749	39.0	37.0	39.0	32.0	39.0
10-11	36.70227985767309	39.0	37.0	39.0	32.0	39.0
12-13	36.64326312693761	39.0	37.0	39.0	32.0	39.0
14-15	37.834874162039256	40.0	38.0	41.0	32.0	41.0
16-17	37.81612534625636	40.0	38.0	41.0	32.0	41.0
18-19	37.82348812528116	40.0	38.0	41.0	32.0	41.0
20-21	37.774483448858675	40.0	38.0	41.0	32.0	41.0
22-23	37.63222046954861	40.0	37.0	41.0	32.0	41.0
24-25	37.549155907485186	40.0	37.0	41.0	32.0	41.0
26-27	37.40127645526478	40.0	37.0	41.0	31.0	41.0
28-29	37.23584966632686	40.0	36.5	41.0	31.0	41.0
30-31	37.051314292091	40.0	36.0	41.0	30.0	41.0
32-33	36.87153519871018	39.0	36.0	41.0	30.0	41.0
34-35	36.69721425640465	39.0	35.5	41.0	30.0	41.0
36-37	36.52662224665891	39.0	35.0	41.0	30.0	41.0
38-39	36.391808646790025	39.0	35.0	40.0	29.0	41.0
40-41	36.349352589236204	39.0	35.0	40.0	29.0	41.0
42-43	36.28168964968097	39.0	35.0	40.5	28.5	41.0
44-45	36.10421331368066	39.0	35.0	40.0	28.0	41.0
46-47	36.05113830144617	39.0	35.0	40.0	27.5	41.0
48-49	36.01709201613906	39.0	35.0	41.0	27.0	41.0
50-51	35.03075779432808	38.0	34.0	40.0	25.5	40.5
52-53	35.23577306273706	38.0	34.5	39.5	26.5	40.5
54-55	35.82207124947546	39.0	35.0	40.5	27.0	41.0
56-57	35.78531838149053	39.0	35.0	41.0	27.0	41.0
58-59	35.628503277447976	39.0	34.5	40.5	26.5	41.0
60-61	35.43376277065536	38.0	34.0	40.0	26.0	41.0
62-63	35.172576728550126	38.0	34.0	40.0	26.0	41.0
64-65	34.8928852817652	37.0	34.0	40.0	26.0	41.0
66-67	34.58851376249167	37.0	34.0	40.0	26.0	41.0
68-69	34.300338613440154	36.0	33.0	39.0	25.5	41.0
70-71	33.955157141977026	36.0	33.0	39.0	25.0	41.0
72-73	33.60670670821936	35.0	33.0	39.0	24.5	40.5
74-75	33.24110125506671	35.0	32.5	37.5	24.0	40.0
76-77	32.90179884757513	35.0	32.0	37.0	24.0	39.0
78-79	32.582907983860935	35.0	32.0	36.5	23.0	39.0
80-81	32.24835592887374	35.0	32.0	36.0	22.5	38.5
82-83	31.950947896772306	35.0	31.0	36.0	22.0	37.0
84-85	31.68622332678115	35.0	31.0	35.0	21.5	37.0
86-87	31.389677695336246	34.5	31.0	35.0	20.0	36.5
88-89	31.167852298049574	34.0	31.0	35.0	20.0	36.0
90-91	30.93747566179573	34.0	30.5	35.0	20.0	36.0
92-93	30.78341305608801	34.0	30.0	35.0	19.0	36.0
94-95	30.619564835813748	34.0	30.0	35.0	19.0	35.0
96-97	30.511829151908756	34.0	30.0	35.0	19.0	35.0
98-99	30.334252847642553	34.0	29.5	35.0	18.0	35.0
100	28.948125071924842	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3336876778407074
1101	2	0.3782745527617877
1101	3	0.329463830732621
1101	4	0.37102242629523374
1101	5	0.31365987871442513
1101	6	0.2968397917130048
1101	7	0.326074060612882
1101	8	0.36805745599929196
1101	9	0.32993394765288286
1101	10-11	0.3716052844350983
1101	12-13	0.38553548686506645
1101	14-15	0.4322150175548529
1101	16-17	0.47094085026703425
1101	18-19	0.3779083169611681
1101	20-21	0.41503961118539223
1101	22-23	0.4790335616212502
1101	24-25	0.36824991054346157
1101	26-27	0.38139399705747223
1101	28-29	0.4331475841127599
1101	30-31	0.4341924023699235
1101	32-33	0.3796115700604048
1101	34-35	0.32977178143974584
1101	36-37	0.3689785128398242
1101	38-39	0.3440303962807647
1101	40-41	0.21716222502993787
1101	42-43	0.12898515531479404
1101	44-45	0.10777406612815099
1101	46-47	0.1151981075876094
1101	48-49	0.19194588268629076
1101	50-51	0.21803540717078107
1101	52-53	0.2474708130645169
1101	54-55	0.3616810449965655
1101	56-57	0.33227368341292873
1101	58-59	0.27404700024248996
1101	60-61	0.21978364361755354
1101	62-63	0.1429493073078305
1101	64-65	0.253615903907054
1101	66-67	0.2029386796410222
1101	68-69	0.1245364209476918
1101	70-71	0.024496761723263205
1101	72-73	0.024553751052501127
1101	74-75	0.0033122269673029336
1101	76-77	0.018725321943705353
1101	78-79	-0.0181094768154324
1101	80-81	-0.05578758027795061
1101	82-83	-0.17501760323833437
1101	84-85	-0.24181973181686445
1101	86-87	-0.23669596000787507
1101	88-89	-0.12550638913409173
1101	90-91	-0.275840193068305
1101	92-93	-0.29106927405283045
1101	94-95	-0.41621580281600856
1101	96-97	-0.38817265263914535
1101	98-99	-0.3678270999967417
1101	100	-0.27313549503417534
1102	1	0.4313203300778241
1102	2	0.4623906083263165
1102	3	0.40699303979954493
1102	4	0.30459098010990715
1102	5	0.32859486498481516
1102	6	0.3375258682757618
1102	7	0.2901795497637991
1102	8	0.2979573779289737
1102	9	0.3851764367499584
1102	10-11	0.3304332874502336
1102	12-13	0.33288126433774323
1102	14-15	0.41253961508890313
1102	16-17	0.45570485581271925
1102	18-19	0.4856571324460077
1102	20-21	0.4444091933745966
1102	22-23	0.4909606533673312
1102	24-25	0.5518606963615795
1102	26-27	0.4675844684406556
1102	28-29	0.48285246485362165
1102	30-31	0.45358866980114243
1102	32-33	0.34372664974596034
1102	34-35	0.4696583102719245
1102	36-37	0.4830096332834799
1102	38-39	0.45412334033934343
1102	40-41	0.3346400490217505
1102	42-43	0.4416209370947968
1102	44-45	0.3779988211488856
1102	46-47	0.3273976041686595
1102	48-49	0.2384805028338164
1102	50-51	0.22226853456000129
1102	52-53	0.39518591024860683
1102	54-55	0.3730764509622091
1102	56-57	0.4371907673519786
1102	58-59	0.3542973973242738
1102	60-61	0.34130917799540583
1102	62-63	0.3922305219742839
1102	64-65	0.4196960336652964
1102	66-67	0.3298552722754948
1102	68-69	0.34599465332802737
1102	70-71	0.34180347038638104
1102	72-73	0.33816238320670067
1102	74-75	0.25633728350099005
1102	76-77	0.2505645509320118
1102	78-79	0.19705147591164973
1102	80-81	0.19681599749624468
1102	82-83	0.23334080008907776
1102	84-85	0.11826698680694747
1102	86-87	0.04305202766240157
1102	88-89	0.01929099820236857
1102	90-91	-0.08895112695082119
1102	92-93	-0.07991430447333059
1102	94-95	-0.10404154448402281
1102	96-97	-0.208078564446609
1102	98-99	-0.17110403425614962
1102	100	-0.2461295589979926
1103	1	0.35105121094450453
1103	2	0.38013489660814415
1103	3	0.2975712563594186
1103	4	0.3620306517203389
1103	5	0.326501481068739
1103	6	0.27124031472824583
1103	7	0.268724076023517
1103	8	0.28086018537101154
1103	9	0.3356079583646263
1103	10-11	0.2956959219351276
1103	12-13	0.28562771831789746
1103	14-15	0.44576416564426324
1103	16-17	0.4497598272105634
1103	18-19	0.33871854617405006
1103	20-21	0.4118093347673124
1103	22-23	0.4317550041307996
1103	24-25	0.37771023509272794
1103	26-27	0.42512701072156034
1103	28-29	0.38214046041269967
1103	30-31	0.35358176197028257
1103	32-33	0.44596995226166314
1103	34-35	0.5302781356220407
1103	36-37	0.5417438697379069
1103	38-39	0.43435736022393456
1103	40-41	0.36266244284030336
1103	42-43	0.3159047008295559
1103	44-45	0.3786291762736198
1103	46-47	0.426175167036142
1103	48-49	0.457407461546147
1103	50-51	0.35614895565141325
1103	52-53	0.47296976608777896
1103	54-55	0.512068604662069
1103	56-57	0.5112187546465492
1103	58-59	0.38454784664444475
1103	60-61	0.35767513620489666
1103	62-63	0.44056816181118563
1103	64-65	0.4133033029437527
1103	66-67	0.3823938690025557
1103	68-69	0.39091845559728
1103	70-71	0.4096031457805651
1103	72-73	0.37443951044521384
1103	74-75	0.3855172679676997
1103	76-77	0.34947112220838505
1103	78-79	0.30190749431261565
1103	80-81	0.32493771472076105
1103	82-83	0.2548539013192901
1103	84-85	0.25305743682412185
1103	86-87	0.19635073471560816
1103	88-89	0.1826710931943758
1103	90-91	0.17260965698218556
1103	92-93	0.08580175754032027
1103	94-95	0.12832743076727482
1103	96-97	0.04126033746966229
1103	98-99	0.059748080909212575
1103	100	0.013964462181043302
1104	1	0.3886909453440808
1104	2	0.35257525058194616
1104	3	0.33360502075311516
1104	4	0.3896953612048719
1104	5	0.25949801097279845
1104	6	0.26360970584048005
1104	7	0.3252121948472819
1104	8	0.3898860369853878
1104	9	0.4153331123576365
1104	10-11	0.4553098287348263
1104	12-13	0.3904525325056696
1104	14-15	0.4185467263109075
1104	16-17	0.4650375549581227
1104	18-19	0.3313896195023389
1104	20-21	0.36087156835639433
1104	22-23	0.41051417466980666
1104	24-25	0.4589092793154421
1104	26-27	0.4598835036432902
1104	28-29	0.4837203146446569
1104	30-31	0.44297875775573203
1104	32-33	0.31019344325021336
1104	34-35	0.41269861759315063
1104	36-37	0.40886110855014834
1104	38-39	0.48701066281870453
1104	40-41	0.43993811734770105
1104	42-43	0.1015992603435052
1104	44-45	0.28412159369374734
1104	46-47	0.31586604032360555
1104	48-49	0.34279973848141054
1104	50-51	0.441985801106064
1104	52-53	0.501713627379992
1104	54-55	0.4802989157699784
1104	56-57	0.49678753819181054
1104	58-59	0.5162133726221327
1104	60-61	0.5101357223989638
1104	62-63	0.48263557052604256
1104	64-65	0.44485536038644824
1104	66-67	0.3471150606022775
1104	68-69	0.24474966965057376
1104	70-71	0.27366153704888774
1104	72-73	0.3542793336984573
1104	74-75	0.34646968322651617
1104	76-77	0.4627430384457938
1104	78-79	0.40054106899172126
1104	80-81	0.31745569558120224
1104	82-83	0.3921778449812585
1104	84-85	0.2115783834758993
1104	86-87	0.21032117286024388
1104	88-89	0.28097747024611763
1104	90-91	0.23675089829193752
1104	92-93	0.4061582528634986
1104	94-95	0.3812692076004822
1104	96-97	0.458096606576202
1104	98-99	0.3064956164206656
1104	100	0.3521607327284535
1105	1	0.2977590897516542
1105	2	0.3823175258334146
1105	3	0.2746987616535286
1105	4	0.2715720624021074
1105	5	0.21826274969303228
1105	6	0.20432653681447022
1105	7	0.284449953257365
1105	8	0.2669399239472412
1105	9	0.4470563644979464
1105	10-11	0.32833642632946436
1105	12-13	0.2883589044514494
1105	14-15	0.4208242873163428
1105	16-17	0.4022531829952882
1105	18-19	0.36211188324059407
1105	20-21	0.45312247262272365
1105	22-23	0.4706502214643322
1105	24-25	0.45898838050818114
1105	26-27	0.4264480780652633
1105	28-29	0.5057370476311362
1105	30-31	0.46267490939199973
1105	32-33	0.4061226663694697
1105	34-35	0.4251991233146555
1105	36-37	0.46343428685965904
1105	38-39	0.4975194875908713
1105	40-41	0.502398714158943
1105	42-43	0.5293161005701563
1105	44-45	0.5022093649940516
1105	46-47	0.5308997980275834
1105	48-49	0.38673008715983315
1105	50-51	0.44202167785116586
1105	52-53	0.42593153247924675
1105	54-55	0.47198691798771364
1105	56-57	0.37964100758645714
1105	58-59	0.41469551870280696
1105	60-61	0.42616592835052813
1105	62-63	0.5080519992995036
1105	64-65	0.4487065089956346
1105	66-67	0.4447777518897311
1105	68-69	0.36289852666554623
1105	70-71	0.38813840239924957
1105	72-73	0.4055271096577542
1105	74-75	0.3975269631941174
1105	76-77	0.40422001646092554
1105	78-79	0.33375643995000814
1105	80-81	0.47825574336848575
1105	82-83	0.36719180108046245
1105	84-85	0.26919362402607305
1105	86-87	0.3551021178909046
1105	88-89	0.4260867487253108
1105	90-91	0.3067142931264506
1105	92-93	0.36064853366728755
1105	94-95	0.29167091466227646
1105	96-97	0.25766382236117025
1105	98-99	0.30256044547228456
1105	100	0.3975062171661108
1106	1	0.39791820574948034
1106	2	0.36414406348082906
1106	3	0.3264576403956454
1106	4	0.2805701789613977
1106	5	0.2708404683429606
1106	6	0.2513535084715457
1106	7	0.28053697685054857
1106	8	0.3437345991368872
1106	9	0.36999287272622183
1106	10-11	0.3626444432949967
1106	12-13	0.38301000362897497
1106	14-15	0.4332130646257042
1106	16-17	0.4480546859808854
1106	18-19	0.3905678862080606
1106	20-21	0.3517937571434544
1106	22-23	0.44926024140159626
1106	24-25	0.4384475576716511
1106	26-27	0.5302865501159886
1106	28-29	0.5121190020007589
1106	30-31	0.5298795087651982
1106	32-33	0.52229123265667
1106	34-35	0.4845317946593539
1106	36-37	0.6200600210618248
1106	38-39	0.5482438559206955
1106	40-41	0.5020742099276561
1106	42-43	0.5091785706845684
1106	44-45	0.48718397945919634
1106	46-47	0.5216872363644072
1106	48-49	0.551501376804552
1106	50-51	0.5090422629018931
1106	52-53	0.5079379271641713
1106	54-55	0.4468977712291533
1106	56-57	0.4786269386714679
1106	58-59	0.5042582553781543
1106	60-61	0.48663763223964196
1106	62-63	0.4911571743886043
1106	64-65	0.5576790010669512
1106	66-67	0.5703654959957731
1106	68-69	0.5383381765713153
1106	70-71	0.5564882021665198
1106	72-73	0.48956739701691987
1106	74-75	0.5498711250294193
1106	76-77	0.5278040944406968
1106	78-79	0.42888896271261956
1106	80-81	0.5063385437773675
1106	82-83	0.4930591861404956
1106	84-85	0.4569794118390682
1106	86-87	0.5268681983577004
1106	88-89	0.5424067881414594
1106	90-91	0.5312768716343506
1106	92-93	0.5277361254378761
1106	94-95	0.6102113254918926
1106	96-97	0.6182434840425906
1106	98-99	0.5737007501640541
1106	100	0.6504809565313465
1107	1	0.14176491910955846
1107	2	0.2060960823335165
1107	3	0.2020749060219842
1107	4	0.160368494029953
1107	5	0.1485646619943921
1107	6	0.11729897875338935
1107	7	0.08268656674802344
1107	8	0.14606287657790062
1107	9	0.07785113560614576
1107	10-11	0.14910257947323657
1107	12-13	0.1806229177974572
1107	14-15	0.14709094160694747
1107	16-17	0.18070318912073446
1107	18-19	0.13867897159630616
1107	20-21	0.1879238206010001
1107	22-23	0.22990118157788686
1107	24-25	0.22447059676513703
1107	26-27	0.26065953202812153
1107	28-29	0.3010214961131723
1107	30-31	0.22477913936102567
1107	32-33	0.24346333350993632
1107	34-35	0.32175536744240674
1107	36-37	0.32620505734899297
1107	38-39	0.28849116196025903
1107	40-41	0.3306019286114861
1107	42-43	0.37252007733490444
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2316	94-95	0.09275525774429028
2316	96-97	0.015230871512045496
2316	98-99	0.21047780464344257
2316	100	-0.006887447166416649
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	6.0
1	31.0
2	51.0
3	77.0
4	70.0
5	80.0
6	131.0
7	856.0
8	1789.0
9	2672.0
10	3692.0
11	5829.0
12	8118.0
13	10146.0
14	11264.0
15	12789.0
16	14733.0
17	17619.0
18	21807.0
19	25896.0
20	30504.0
21	35377.0
22	40802.0
23	43434.0
24	44063.0
25	44727.0
26	47726.0
27	54070.0
28	62525.0
29	74271.0
30	88964.0
31	109912.0
32	138501.0
33	180602.0
34	251103.0
35	358012.0
36	541012.0
37	865354.0
38	916955.0
39	235425.0
40	370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.506114450782256	18.14145845631512	12.202308390006834	24.150118702895792
2	15.943277582757814	20.274396345726178	40.35561122235162	23.426714849164394
3	18.33015625105376	26.08203546595902	30.42382827988494	25.163980003102278
4	12.246737076329229	16.293222895559	38.14656726150692	33.31347276660485
5	12.559910477874576	37.56759622768051	35.46497972403856	14.407513570406353
6	30.563175396295655	36.75948341780836	18.688545781002873	13.988795404893112
7	26.84957233997885	32.66583697514901	22.942101931160096	17.54248875371205
8	25.741943523757442	35.387860323412234	20.907174290699015	17.963021862131306
9	26.892276330143154	15.039166579092496	20.495564407005066	37.57299268375928
10-11	24.524100087255388	25.705209772138588	29.560489048669815	20.210201091936213
12-13	25.028397079463115	23.815730476579194	29.194731947961184	21.96114049599651
14-15	22.463793474483627	25.204373627602678	26.885716209015843	25.446116688897852
16-17	22.015453752726238	27.95190868970466	27.54594746161178	22.486690095957325
18-19	21.76497581359324	27.161252826456035	29.370193106429376	21.70357825352135
20-21	22.568808150086372	26.54364478399262	29.302004805357	21.585542260564008
22-23	22.267633847450973	26.416153275864573	29.188748528150484	22.127464348533966
24-25	21.9894480839382	26.39728716169595	29.283293520375757	22.329971233990094
26-27	21.871121849335907	26.56558562741597	29.255696169568573	22.30759635367955
28-29	21.954862547164733	26.913224566042604	29.14271489101547	21.989197995777193
30-31	22.511407986811495	26.539587022157946	29.213119213898153	21.735885777132406
32-33	22.17474474298855	26.644298821177024	29.18408559339996	21.996870842434465
34-35	22.276949721416052	26.85765070518561	29.061348462120325	21.804051111278017
36-37	22.183046680761333	26.896873904182918	28.934616983734703	21.985462431321046
38-39	22.15085514921861	27.047198302723196	28.831324746496716	21.970621801561478
40-41	22.2479092308474	26.76906562760049	28.981658483656208	22.0013666578959
42-43	22.09136233253813	26.924983034623324	28.730440978861537	22.253213653977014
44-45	21.945088497525298	27.153958797476957	28.77412359780125	22.126829107196492
46-47	21.929990189474065	26.903710583206585	28.562954440233806	22.603344787085543
48-49	22.046501418866075	27.02247852634636	28.54741805204086	22.383602002746702
50-51	22.085649497743756	27.29980230617606	28.43776539131577	22.176782804764418
52-53	22.0405803275414	27.359555728509694	28.270928965649627	22.32893497829928
54-55	21.957702932684878	27.217436933386463	28.474996189196762	22.3498639447319
56-57	22.069880162152504	27.3266404210003	28.172073164649063	22.431406252198137
58-59	22.016792398818772	27.326389752850922	28.34417255252508	22.312645295805225
60-61	22.292435080042893	27.36562024083677	28.32449316401322	22.01745151510712
62-63	22.136536056806637	27.378049858392785	28.366985980699738	22.11842810410084
64-65	22.0397189005181	27.16632982898894	28.303388093632076	22.490563176860885
66-67	21.96248621380914	27.224759266308396	28.22550359853589	22.58725092134657
68-69	21.841456032731248	27.30475302152232	28.35090563697362	22.502885308772804
70-71	21.921777975003913	27.143502712905963	28.38509705363225	22.549622258457873
72-73	21.899827826678703	27.459819054049117	28.02246974640542	22.61788337286676
74-75	21.94921964441727	27.478114913117263	28.03486636023262	22.537799082232855
76-77	21.924874604979525	27.288572484949107	28.065033334030943	22.721519576040425
78-79	21.856359305310388	27.287567508619908	28.147029713733108	22.709043472336592
80-81	21.932618658786502	27.453813221455864	27.90195560014036	22.711612519617276
82-83	22.120697817610903	27.450944145571977	27.889789071684746	22.53856896513237
84-85	22.040503648872107	27.58759845048645	27.774864085050943	22.597033815590496
86-87	21.89326842852798	27.54075618097819	27.911693933567918	22.654281456925922
88-89	21.809005556753704	27.29431611353938	28.03875485467511	22.857923475031814
90-91	22.213227834670963	27.181947403762024	27.955327789033678	22.649496972533335
92-93	22.03482256990687	27.281485734457267	27.943190372110315	22.740501323525546
94-95	21.986107169444193	27.20517904223481	27.865290211387418	22.94342357693358
96-97	21.9427525777151	27.10829716762327	27.985970068394174	22.962980186267455
98-99	21.97392850038361	27.05177060903063	27.87721424225686	23.0970866483289
100	22.085972043491417	27.060868586361497	27.855452835573114	22.997706534573975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2475.0
1	1755.5
2	2577.5
3	3692.5
4	2369.5
5	1662.0
6	2475.0
7	4371.5
8	6741.0
9	7759.5
10	6317.0
11	4765.0
12	4620.0
13	4850.0
14	5069.5
15	5264.5
16	5619.5
17	6060.0
18	6652.5
19	7207.5
20	7951.5
21	9212.0
22	10934.0
23	13339.0
24	16673.5
25	20957.5
26	26069.5
27	31875.0
28	38111.0
29	44765.5
30	52313.5
31	59519.0
32	66969.0
33	75115.0
34	83266.5
35	91536.0
36	101734.5
37	111383.5
38	119128.0
39	126444.5
40	130817.5
41	134079.0
42	138034.5
43	142779.5
44	147111.5
45	151920.5
46	155988.5
47	158430.5
48	162072.0
49	165801.5
50	167340.0
51	163441.0
52	155888.5
53	147964.5
54	139483.5
55	129243.0
56	117405.0
57	105630.0
58	93658.0
59	80370.0
60	66430.0
61	54324.5
62	44132.5
63	34478.0
64	26106.0
65	19869.0
66	15050.5
67	11678.5
68	9232.5
69	7279.5
70	5807.5
71	4526.5
72	3546.0
73	2929.0
74	2334.5
75	1854.0
76	1494.5
77	1068.5
78	773.0
79	591.0
80	447.0
81	353.5
82	276.5
83	211.5
84	163.0
85	111.5
86	87.0
87	77.5
88	65.5
89	46.0
90	41.0
91	41.5
92	30.5
93	24.0
94	16.5
95	12.5
96	10.5
97	6.0
98	4.5
99	2.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16343649050940806
2	0.10157240782867763
3	0.0301764672377257
4	0.03224557785726159
5	0.027874872278916107
6	0.0021853527891727395
7	0.0039057368997980875
8	0.005323891369367631
9	0.0057888600479150225
10-11	0.004603189917619174
12-13	0.007125644998738772
14-15	0.01684349038037925
16-17	0.007869594884414598
18-19	0.01640177013575923
20-21	0.017389828577672434
22-23	0.044811356395004845
24-25	0.014634889157279143
26-27	0.00991545707002312
28-29	0.02660783262987447
30-31	0.015111482052790217
32-33	0.01967979931951834
34-35	0.020133143781102043
36-37	0.02141180764710737
38-39	0.01927295172578937
40-41	0.02542216249957862
42-43	0.03326850895006585
44-45	0.03616293897402336
46-47	0.02050511872393996
48-49	0.013286479989491708
50-51	0.012147306727050599
52-53	0.02165591620334475
54-55	0.025352417197796517
56-57	0.01965655088559097
58-59	0.025247799245123353
60-61	0.02575926479152548
62-63	0.049960884509917196
64-65	0.030722805435018884
66-67	0.020133143781102043
68-69	0.02631722720578235
70-71	0.04026628756220409
72-73	0.037848450433757654
74-75	0.03930147755421825
76-77	0.022202254400637937
78-79	0.01651801230539608
80-81	0.021993018495291613
82-83	0.01759906448301876
84-85	0.01835463858565827
86-87	0.021225820175688415
88-89	0.017261962191071904
90-91	0.026793820101293428
92-93	0.02575926479152548
94-95	0.01863361979278671
96-97	0.013019122999326959
98-99	0.017866421473183514
100	0.0408707468443157
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4301365.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.69589617480698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33131871542935	52.24377500279991
2	14.13133147107505	18.85003633213208
3	3.905955871814401	7.81533681869733
4	1.4231847593138696	3.79682331779062
5	0.7063704712307436	2.355600580507758
6	0.3901839911703053	1.5614202578499892
7	0.24554557594098264	1.1463817567400072
8	0.1623369747610594	0.8661768011196703
9	0.1220056153524544	0.7323546468861661
>10	0.5430022413094291	6.147986847531717
>50	0.025784498500011323	1.1659939615428605
>100	0.012058192986593348	1.4419880010062893
>500	4.6081056164615035E-4	0.22603587414676443
>1k	2.9324307986066625E-4	0.42773586588300005
>5k	8.378373710304751E-5	0.4018912205148
>10k+	8.378373710304751E-5	0.8204627148510221
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15746	0.3660698406203612	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13619	0.3166204216568461	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8963	0.20837571329101343	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5421	0.1260297603202704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.3248433927369566E-5	0.0
3	0.0	0.0	0.0	2.3248433927369566E-5	0.0
4	0.0	0.0	0.0	2.3248433927369566E-5	2.3248433927369566E-5
5	0.0	0.0	0.0	2.3248433927369566E-5	2.3248433927369566E-5
6	0.0	0.0	0.0	2.3248433927369566E-5	2.3248433927369566E-5
7	0.0	0.0	0.0	2.3248433927369566E-5	2.3248433927369566E-5
8	0.0	0.0	0.0	2.3248433927369566E-5	2.3248433927369566E-5
9	0.0	0.0	0.0	2.3248433927369566E-5	2.3248433927369566E-5
10-11	0.0	0.0	0.0	5.812108481842392E-5	2.3248433927369566E-5
12-13	0.0	2.3248433927369566E-5	0.0	6.97453017821087E-5	2.3248433927369566E-5
14-15	0.0	3.487265089105435E-5	0.0	6.97453017821087E-5	2.3248433927369566E-5
16-17	0.0	4.649686785473913E-5	0.0	6.97453017821087E-5	2.3248433927369566E-5
18-19	0.0	4.649686785473913E-5	0.0	6.97453017821087E-5	4.649686785473913E-5
20-21	0.0	6.97453017821087E-5	0.0	1.2786638660053262E-4	4.649686785473913E-5
22-23	0.0	6.97453017821087E-5	0.0	2.5573277320106524E-4	4.649686785473913E-5
24-25	0.0	6.97453017821087E-5	0.0	5.114655464021305E-4	4.649686785473913E-5
26-27	0.0	8.136951874579349E-5	0.0	7.55574102639511E-4	4.649686785473913E-5
28-29	0.0	9.299373570947827E-5	0.0	0.0017901294124074568	4.649686785473913E-5
30-31	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.006230580292535044	4.649686785473913E-5
32-33	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.014902246147443893	4.649686785473913E-5
34-35	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.029060542409211958	4.649686785473913E-5
36-37	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.0507280828295204	4.649686785473913E-5
38-39	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.08798369819813012	4.649686785473913E-5
40-41	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.13531750967425457	4.649686785473913E-5
42-43	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.17950115835322045	4.649686785473913E-5
44-45	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.23019436853184977	4.649686785473913E-5
46-47	2.3248433927369566E-5	9.299373570947827E-5	0.0	0.2954062256981214	4.649686785473913E-5
48-49	2.3248433927369566E-5	1.1624216963684784E-4	0.0	0.3665348092989086	6.97453017821087E-5
50-51	2.3248433927369566E-5	1.394906035642174E-4	0.0	0.44502152223770824	6.97453017821087E-5
52-53	2.3248433927369566E-5	1.394906035642174E-4	0.0	0.5262980472477923	9.299373570947827E-5
54-55	2.3248433927369566E-5	1.9761168838264132E-4	0.0	0.6220932192455185	1.1624216963684784E-4
56-57	2.3248433927369566E-5	2.0923590534632612E-4	0.0	0.7290941363962371	1.1624216963684784E-4
58-59	2.3248433927369566E-5	2.0923590534632612E-4	0.0	0.8526130658523515	1.2786638660053262E-4
60-61	2.3248433927369566E-5	2.0923590534632612E-4	0.0	0.9860474523784892	1.394906035642174E-4
62-63	2.3248433927369566E-5	2.2086012231001088E-4	0.0	1.1278977719863346	1.5111482052790218E-4
64-65	2.3248433927369566E-5	2.4410855623738047E-4	0.0	1.2679812106156998	1.7436325445527176E-4
66-67	2.3248433927369566E-5	3.0222964105580436E-4	0.0	1.433184582103588	1.9761168838264132E-4
68-69	2.3248433927369566E-5	3.0222964105580436E-4	0.0	1.6309473853067573	2.0923590534632612E-4
70-71	4.649686785473913E-5	3.0222964105580436E-4	0.0	1.849598906393668	2.3248433927369568E-4
72-73	4.649686785473913E-5	3.0222964105580436E-4	0.0	2.0652048826360936	2.3248433927369568E-4
74-75	4.649686785473913E-5	3.0222964105580436E-4	0.0	2.2774049633081592	2.3248433927369568E-4
76-77	4.649686785473913E-5	3.0222964105580436E-4	0.0	2.5167708390243564	2.4410855623738047E-4
78-79	4.649686785473913E-5	3.0222964105580436E-4	0.0	2.7937410566180736	2.5573277320106524E-4
80-81	4.649686785473913E-5	3.138538580194891E-4	0.0	3.0872409107341507	2.5573277320106524E-4
82-83	4.649686785473913E-5	3.2547807498317394E-4	0.0	3.3949223095459233	2.5573277320106524E-4
84-85	4.649686785473913E-5	3.2547807498317394E-4	0.0	3.707009286586932	2.6735699016475006E-4
86-87	4.649686785473913E-5	3.4872650891054353E-4	0.0	4.035091186169971	2.789812071284348E-4
88	4.649686785473913E-5	3.4872650891054353E-4	0.0	4.299937345470566	3.0222964105580436E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7940	0.0	48.44483	1
TACCTGG	4130	0.0	39.052956	2
GTATCAA	13200	0.0	36.8088	1
GTACCTG	4805	0.0	34.490158	1
ACCTGGG	5340	0.0	30.094458	3
TCAACGC	15950	0.0	29.961388	4
ATCAACG	16005	0.0	29.946507	3
CAACGCA	16195	0.0	29.448729	5
TATCAAC	16720	0.0	28.630001	2
AACGCAG	16670	0.0	28.578098	6
ATAACGC	375	0.0	27.567228	3
GTACATG	14510	0.0	27.093489	1
TACATGG	14695	0.0	26.239414	2
TAACGCA	405	0.0	25.52521	4
ACGCAGA	18640	0.0	25.331224	7
ACATGGG	14795	0.0	24.868435	3
CGCAGAG	19160	0.0	24.4239	8
CATGGGG	9440	0.0	23.146355	4
ATGGGGG	6090	0.0	21.912025	5
GCAGAGT	21045	0.0	21.612394	9
>>END_MODULE