FastQCFastQC Report
Mon 6 Jun 2016
ERR1211551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4301365
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171650.3990593683632986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154910.36014148996888196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111340.2588480633473328No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53550.12449536368106402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82600.045.542081
TACCTGG40700.039.164092
GTATCAA139800.036.998871
GTACCTG49750.032.9864271
ACCTGGG49450.032.213913
TAACGCA5450.029.3151724
TCAACGC173550.029.2692184
ATCAACG174050.029.0501423
CAACGCA177000.028.7252645
TATCAAC180950.027.9339662
AACGCAG184150.027.7630526
GTACATG147500.027.7351281
TATAACG4500.027.1671182
TACATGG150200.026.5778882
CATGGGG96200.025.937584
ATGGGGG64250.025.6710725
ACATGGG152150.025.5413343
ACGCAGA207350.024.498067
CGCAGAG212300.023.8161938
TGGGGGG58400.023.1733976
Produced by FastQC (version 0.11.5)