##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211551_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4301365 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10640203749275 33.0 31.0 34.0 30.0 34.0 2 32.319939833053 34.0 31.0 34.0 30.0 34.0 3 32.33041464744331 34.0 31.0 34.0 30.0 34.0 4 35.97360837780565 37.0 35.0 37.0 35.0 37.0 5 35.90025584901537 37.0 35.0 37.0 35.0 37.0 6 35.92115014652325 37.0 35.0 37.0 35.0 37.0 7 35.84495619413837 37.0 35.0 37.0 35.0 37.0 8 35.84043669858289 37.0 35.0 37.0 35.0 37.0 9 37.49200265497115 39.0 37.0 39.0 35.0 39.0 10-11 37.453464074776264 39.0 37.0 39.0 34.5 39.0 12-13 37.36126462181191 39.0 37.0 39.0 34.0 39.0 14-15 38.58745642371666 40.0 38.0 41.0 34.0 41.0 16-17 38.512328179543005 40.0 38.0 41.0 34.0 41.0 18-19 38.50096376382846 40.0 38.0 41.0 34.0 41.0 20-21 38.43980492239092 40.0 38.0 41.0 34.0 41.0 22-23 38.32621295797962 40.0 38.0 41.0 34.0 41.0 24-25 38.21525573858531 40.0 38.0 41.0 33.5 41.0 26-27 38.031600085089266 40.0 37.0 41.0 33.0 41.0 28-29 38.06115663283632 40.0 37.5 41.0 33.0 41.0 30-31 37.97239248471125 40.0 37.0 41.0 33.0 41.0 32-33 37.7722353252979 40.0 37.0 41.0 33.0 41.0 34-35 37.64030580989988 40.0 37.0 41.0 33.0 41.0 36-37 37.576451545032796 40.0 37.0 41.0 32.5 41.0 38-39 37.47115369190943 40.0 36.5 41.0 32.0 41.0 40-41 37.30026433469375 40.0 36.0 41.0 31.0 41.0 42-43 37.14450447706716 40.0 36.0 41.0 31.0 41.0 44-45 36.95502148736506 39.0 36.0 41.0 30.5 41.0 46-47 36.69208042098264 39.0 35.0 41.0 30.0 41.0 48-49 36.46579527661568 39.0 35.0 41.0 29.5 41.0 50-51 36.25539508969827 39.0 35.0 40.5 29.0 41.0 52-53 36.05483759225269 39.0 35.0 40.0 28.0 41.0 54-55 35.85890571946347 38.5 35.0 40.0 28.0 41.0 56-57 35.65794172315067 38.0 34.0 40.0 27.5 41.0 58-59 35.403028805972056 38.0 34.0 40.0 27.0 41.0 60-61 35.08321904790689 38.0 34.0 40.0 26.0 41.0 62-63 34.753407232355315 37.0 33.5 40.0 26.0 41.0 64-65 34.43747833536563 37.0 33.0 39.5 26.0 41.0 66-67 34.09772827927878 36.0 33.0 39.0 25.0 41.0 68-69 33.702518851573856 36.0 32.0 39.0 24.5 40.0 70-71 33.31600968529757 35.0 32.0 38.5 24.0 40.0 72-73 32.90852008606571 35.0 31.0 37.5 23.0 40.0 74-75 32.5042173821566 35.0 31.0 37.0 22.0 39.0 76-77 30.95782420231717 33.5 29.5 35.5 20.5 38.0 78-79 31.80765350069106 34.0 30.5 36.0 22.0 38.5 80-81 31.78583705405145 34.0 31.0 36.0 22.0 37.5 82-83 31.50624790037581 34.0 31.0 35.0 20.5 37.0 84-85 31.210485973638598 34.0 30.0 35.0 20.0 37.0 86-87 30.917627543814582 34.0 30.0 35.0 20.0 36.0 88-89 30.64580429700804 34.0 30.0 35.0 19.5 36.0 90-91 30.359823451392757 34.0 29.0 35.0 19.0 35.5 92-93 30.149480223138468 34.0 29.0 35.0 18.0 35.0 94-95 29.93193614120169 34.0 29.0 35.0 18.0 35.0 96-97 29.726223303532716 34.0 29.0 35.0 17.5 35.0 98-99 29.56552664096165 33.5 29.0 35.0 17.0 35.0 100 27.702381220845012 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31999743027932226 1101 2 0.21846745845148519 1101 3 0.2733749152961451 1101 4 0.12740181119453808 1101 5 0.16919254646992954 1101 6 0.15126979915947203 1101 7 0.14005993564013153 1101 8 0.1363642986659528 1101 9 0.17759465448234835 1101 10-11 0.18463123120567104 1101 12-13 0.18571486605525678 1101 14-15 0.2886289967682387 1101 16-17 0.3127159721534696 1101 18-19 0.3323445574752881 1101 20-21 0.2592210443432279 1101 22-23 0.26885586291554375 1101 24-25 0.179992046936448 1101 26-27 0.16967951625746736 1101 28-29 0.2719593237415907 1101 30-31 0.3029982690473716 1101 32-33 0.3689819339027238 1101 34-35 0.40860530539060846 1101 36-37 0.307031147982606 1101 38-39 0.28875241530485596 1101 40-41 0.35089136866723436 1101 42-43 0.3000229902104721 1101 44-45 0.28168236834797256 1101 46-47 0.361755910824769 1101 48-49 0.3567261387623759 1101 50-51 0.3132023639318078 1101 52-53 0.26726794197598736 1101 54-55 0.2548122819687606 1101 56-57 0.2742835995459316 1101 58-59 0.2862015631228729 1101 60-61 0.34818731916892176 1101 62-63 0.25898832449913556 1101 64-65 0.21213646179531054 1101 66-67 0.1655493916034274 1101 68-69 0.10235860685675391 1101 70-71 0.17093646098241777 1101 72-73 0.08254094251930155 1101 74-75 0.19843883324836753 1101 76-77 0.2597939139028149 1101 78-79 0.3094663617810731 1101 80-81 0.3574480002343776 1101 82-83 0.27136496075349115 1101 84-85 0.23998956520891568 1101 86-87 0.12774412265238055 1101 88-89 0.10280247861890146 1101 90-91 -0.0209300604808611 1101 92-93 -0.15562849009686985 1101 94-95 0.009505963462697054 1101 96-97 -0.05833442234470709 1101 98-99 -0.1767248858428161 1101 100 -0.1465143622449041 1102 1 0.3245120482731849 1102 2 0.2749520059128585 1102 3 0.3334533894252587 1102 4 0.22630882675476016 1102 5 0.18479651459570334 1102 6 0.14913531630801913 1102 7 0.1494913010630583 1102 8 0.16129464109851455 1102 9 0.21589319268296236 1102 10-11 0.22549935967171564 1102 12-13 0.27048980817554025 1102 14-15 0.324743248639237 1102 16-17 0.35977033197687547 1102 18-19 0.32113187643291496 1102 20-21 0.32452736938495974 1102 22-23 0.3495109132574328 1102 24-25 0.26644768818987785 1102 26-27 0.2683746987804838 1102 28-29 0.32693832551836266 1102 30-31 0.42613316244100474 1102 32-33 0.5348091764052825 1102 34-35 0.49682257642921 1102 36-37 0.5189673254983589 1102 38-39 0.4648201614955667 1102 40-41 0.43825575514222237 1102 42-43 0.4195091058083804 1102 44-45 0.4018655643852327 1102 46-47 0.3506492304284947 1102 48-49 0.32503329161866645 1102 50-51 0.37815434395657377 1102 52-53 0.39460685464491974 1102 54-55 0.47465173305366193 1102 56-57 0.43635118421184416 1102 58-59 0.4750447427384472 1102 60-61 0.34503337930998157 1102 62-63 0.42486940859971156 1102 64-65 0.4144859745288514 1102 66-67 0.40882339822594105 1102 68-69 0.43654045012386433 1102 70-71 0.3612437955541097 1102 72-73 0.4001412561466111 1102 74-75 0.40347739729294574 1102 76-77 0.44918341748328316 1102 78-79 0.5283782230851237 1102 80-81 0.527728279403064 1102 82-83 0.4639898396098978 1102 84-85 0.4244478514308483 1102 86-87 0.36608724842635354 1102 88-89 0.3899589019215455 1102 90-91 0.3361183094647586 1102 92-93 0.2305759464508057 1102 94-95 0.39870584366514095 1102 96-97 0.38808914950690365 1102 98-99 0.355980183343636 1102 100 0.3619916007120736 1103 1 0.31836258521700955 1103 2 0.26952742946868113 1103 3 0.35279805409052045 1103 4 0.16998635676337415 1103 5 0.16434622359555107 1103 6 0.2065973916942383 1103 7 0.18947158574037104 1103 8 0.19500112176405793 1103 9 0.25019001284038467 1103 10-11 0.2251329334722456 1103 12-13 0.27198644263055627 1103 14-15 0.32884407309818897 1103 16-17 0.309410564677421 1103 18-19 0.3022963375723222 1103 20-21 0.29545890593547597 1103 22-23 0.29381487773002846 1103 24-25 0.32522747898839555 1103 26-27 0.3126142523977009 1103 28-29 0.35994406586357997 1103 30-31 0.38762636165039766 1103 32-33 0.4528529284900742 1103 34-35 0.48751853727736716 1103 36-37 0.4901880574546098 1103 38-39 0.3806449440544739 1103 40-41 0.390894422067781 1103 42-43 0.40416855596993884 1103 44-45 0.4219885735608244 1103 46-47 0.5013931602499113 1103 48-49 0.516014838375348 1103 50-51 0.4725942869016322 1103 52-53 0.48349713339767675 1103 54-55 0.4531363292131587 1103 56-57 0.5117995086583491 1103 58-59 0.5565913127203714 1103 60-61 0.518947667442454 1103 62-63 0.5688150993579129 1103 64-65 0.5289903034361956 1103 66-67 0.47455109775645354 1103 68-69 0.5382550857637938 1103 70-71 0.42575982421102765 1103 72-73 0.3508024786957691 1103 74-75 0.32038141951749566 1103 76-77 0.32833029414800663 1103 78-79 0.447768255396344 1103 80-81 0.3762915750482705 1103 82-83 0.4362985860439643 1103 84-85 0.34153122832707794 1103 86-87 0.2953430945671798 1103 88-89 0.4170473694534955 1103 90-91 0.3719653464409447 1103 92-93 0.22594620618524885 1103 94-95 0.26151321102233993 1103 96-97 0.3306771714526455 1103 98-99 0.44295375684545135 1103 100 0.5483419758508532 1104 1 0.23166121759096825 1104 2 0.1734814176775643 1104 3 0.23147077241238634 1104 4 0.04349895837474804 1104 5 0.11546469915478497 1104 6 0.13024327380703227 1104 7 0.17688198161948065 1104 8 0.1658535166105395 1104 9 0.2506492902439348 1104 10-11 0.18899560652677394 1104 12-13 0.2524266285028247 1104 14-15 0.2742876210011218 1104 16-17 0.17670340955572783 1104 18-19 0.27158178598316596 1104 20-21 0.1516225287636601 1104 22-23 0.2022550756095427 1104 24-25 0.241226242241396 1104 26-27 0.2897164221082065 1104 28-29 0.3262937410674027 1104 30-31 0.2573806932666969 1104 32-33 0.2191722853816387 1104 34-35 0.24686468234898484 1104 36-37 0.22800970172774981 1104 38-39 0.25319996728850924 1104 40-41 0.3329774402107404 1104 42-43 0.3211759284228961 1104 44-45 0.2854601103690584 1104 46-47 0.22976209508655643 1104 48-49 0.2970986129752049 1104 50-51 0.2713542379120426 1104 52-53 0.29238069907134445 1104 54-55 0.24235051458112622 1104 56-57 0.28439715763833107 1104 58-59 0.35245751590709773 1104 60-61 0.3222883852359999 1104 62-63 0.19385112111505265 1104 64-65 0.33295539835351917 1104 66-67 0.3030049839036906 1104 68-69 0.2835992736532802 1104 70-71 0.24992562035189536 1104 72-73 0.2892683120591215 1104 74-75 0.2919767631050192 1104 76-77 0.30322012760741757 1104 78-79 0.3968770170884035 1104 80-81 0.49123700208620136 1104 82-83 0.34289867249617956 1104 84-85 0.29248010144644354 1104 86-87 0.5085258569556643 1104 88-89 0.42228403239791135 1104 90-91 0.3284825828517377 1104 92-93 0.42800124743016 1104 94-95 0.3920650643417467 1104 96-97 0.40019616092228816 1104 98-99 0.3875643016144892 1104 100 0.48797251269510866 1105 1 0.2358519026272532 1105 2 0.1546622279459129 1105 3 0.1953192595204385 1105 4 0.11562514037969862 1105 5 0.10309624797609729 1105 6 0.1388897961575637 1105 7 0.14429074809987696 1105 8 0.0777337900611883 1105 9 0.1920729974156714 1105 10-11 0.20911064524190692 1105 12-13 0.18564272516012892 1105 14-15 0.296205023934796 1105 16-17 0.2062172710419219 1105 18-19 0.24840776716904855 1105 20-21 0.19723772377621884 1105 22-23 0.29563999797879603 1105 24-25 0.2814168824657699 1105 26-27 0.2880722602751504 1105 28-29 0.38306599149814957 1105 30-31 0.3147112330216686 1105 32-33 0.3229271291957829 1105 34-35 0.44494438750473364 1105 36-37 0.4112117833228055 1105 38-39 0.39608983139592624 1105 40-41 0.3900274519398508 1105 42-43 0.4077346537108113 1105 44-45 0.4155489159718897 1105 46-47 0.4691778656510053 1105 48-49 0.47525481859984353 1105 50-51 0.4842421943381652 1105 52-53 0.37517656157096724 1105 54-55 0.3449810238385922 1105 56-57 0.34735875890772405 1105 58-59 0.3770781930314513 1105 60-61 0.4407512885001452 1105 62-63 0.35322272110176556 1105 64-65 0.2995621540308022 1105 66-67 0.43723369828978065 1105 68-69 0.4403639586717816 1105 70-71 0.49861496257286575 1105 72-73 0.5336138467514289 1105 74-75 0.5719248874179854 1105 76-77 0.35870176769445195 1105 78-79 0.43945130644310737 1105 80-81 0.5007180783050984 1105 82-83 0.49305135503352204 1105 84-85 0.5487034013921814 1105 86-87 0.46682084163161974 1105 88-89 0.4972017290582649 1105 90-91 0.5586766465612172 1105 92-93 0.5279895546047619 1105 94-95 0.5186342750733104 1105 96-97 0.5518601343384795 1105 98-99 0.6171701182372402 1105 100 0.76905529009764 1106 1 0.19071200249921816 1106 2 0.1582266913468544 1106 3 0.17893529050146384 1106 4 0.12497344919752607 1106 5 0.10374602798719224 1106 6 0.12729137953851932 1106 7 0.1487443530945427 1106 8 0.12363384702415914 1106 9 0.17584656045080038 1106 10-11 0.15112394029372922 1106 12-13 0.20377028037565736 1106 14-15 0.24945839670242265 1106 16-17 0.27215303617819586 1106 18-19 0.2501085551152471 1106 20-21 0.2555424201784646 1106 22-23 0.2694504187945199 1106 24-25 0.25068401711746446 1106 26-27 0.295571394211926 1106 28-29 0.34029860307507676 1106 30-31 0.3324899575348681 1106 32-33 0.38039666970824015 1106 34-35 0.3446823832422439 1106 36-37 0.35648364547922284 1106 38-39 0.38364756761991714 1106 40-41 0.4004534778872113 1106 42-43 0.3675391995930042 1106 44-45 0.49195062334523243 1106 46-47 0.4718775593144713 1106 48-49 0.4455930214482109 1106 50-51 0.4755683096989216 1106 52-53 0.45468538561257077 1106 54-55 0.31428841516904527 1106 56-57 0.3561282701784805 1106 58-59 0.46266750020402725 1106 60-61 0.5015842586383528 1106 62-63 0.4966521616959625 1106 64-65 0.5409572435601504 1106 66-67 0.6292328671958742 1106 68-69 0.4419360568630424 1106 70-71 0.49575099715203663 1106 72-73 0.5252601774413961 1106 74-75 0.489965103340829 1106 76-77 0.43902049075804683 1106 78-79 0.5090797481905831 1106 80-81 0.5991139563116548 1106 82-83 0.4831295360452508 1106 84-85 0.5020136645170012 1106 86-87 0.4118685008645997 1106 88-89 0.5152031814756661 1106 90-91 0.5454936508189405 1106 92-93 0.5245635614709094 1106 94-95 0.5484875070288027 1106 96-97 0.6486193083113712 1106 98-99 0.7685242017780674 1106 100 0.7425067139246266 1107 1 0.14442564189739215 1107 2 0.1005575003423047 1107 3 0.21191221833574048 1107 4 0.11731835263785229 1107 5 0.06582150062924796 1107 6 0.07884053545902958 1107 7 0.0779384842270261 1107 8 0.10350515568860885 1107 9 0.14503376190679518 1107 10-11 0.1619061574121048 1107 12-13 0.17154655418519127 1107 14-15 0.1708365859220038 1107 16-17 0.1449469633518632 1107 18-19 0.101697171939513 1107 20-21 0.1073663281202002 1107 22-23 0.22804079809957045 1107 24-25 0.24597066828577852 1107 26-27 0.2268664100469877 1107 28-29 0.17875379613310116 1107 30-31 0.1969407569867414 1107 32-33 0.2144568841341723 1107 34-35 0.1810284124672279 1107 36-37 0.20712600638972134 1107 38-39 0.27243048806592185 1107 40-41 0.29250324582140763 1107 42-43 0.3273573473850462 1107 44-45 0.3397295783533778 1107 46-47 0.32892987097830684 1107 48-49 0.44679170229495213 1107 50-51 0.4215533365151103 1107 52-53 0.4237671499067872 1107 54-55 0.4103595117372194 1107 56-57 0.4781624100363686 1107 58-59 0.5670567100088348 1107 60-61 0.5501761578500819 1107 62-63 0.5100050266934915 1107 64-65 0.6078788277536162 1107 66-67 0.6501561779643694 1107 68-69 0.5250256760597836 1107 70-71 0.6218791891718993 1107 72-73 0.5117974036661508 1107 74-75 0.5078499953577236 1107 76-77 0.47282860787435865 1107 78-79 0.5681920978950323 1107 80-81 0.6013069076016393 1107 82-83 0.5965963475568579 1107 84-85 0.6168272293454464 1107 86-87 0.541415818818141 1107 88-89 0.5849899867819026 1107 90-91 0.41037226437715546 1107 92-93 0.5379876624093605 1107 94-95 0.5201112742961556 1107 96-97 0.5872754451637157 1107 98-99 0.7264597771264967 1107 100 0.7727376711165199 1108 1 0.22688702484105505 1108 2 0.12372086443833297 1108 3 0.19441476633193844 1108 4 0.12229707250399713 1108 5 0.08859373467721099 1108 6 0.12670417205635687 1108 7 0.09664438403709852 1108 8 0.11207154639104999 1108 9 0.07067490937288312 1108 10-11 0.1240125476217031 1108 12-13 0.15548690953426103 1108 14-15 0.16356360577961482 1108 16-17 0.20125552981347994 1108 18-19 0.26275714633634095 1108 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78-79 -0.13413036821325264 2316 80-81 -0.049845492719526874 2316 82-83 -0.05633185929674411 2316 84-85 -0.11693281543477241 2316 86-87 -0.10323458161063215 2316 88-89 -0.20519210145166866 2316 90-91 4.725603487614194E-4 2316 92-93 0.10451559936235455 2316 94-95 0.1908097622015461 2316 96-97 0.17492061084153931 2316 98-99 -0.011046639594223251 2316 100 -0.10634403870660947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 8.0 11 19.0 12 53.0 13 146.0 14 479.0 15 1292.0 16 2896.0 17 6260.0 18 11317.0 19 16887.0 20 22781.0 21 29789.0 22 37964.0 23 42902.0 24 45717.0 25 49118.0 26 56372.0 27 67539.0 28 80858.0 29 97150.0 30 117025.0 31 140690.0 32 173426.0 33 218026.0 34 287333.0 35 389004.0 36 564439.0 37 865463.0 38 845342.0 39 131054.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08875402326215 19.65532698043074 13.3313308081677 23.92458818813941 2 15.369296582811975 21.324994393246957 39.81245992683769 23.49324909710338 3 17.42311345911435 27.041027698823015 30.948927984734055 24.58693085732858 4 12.069214838566285 17.330887269338895 37.837302491414285 32.76259540068053 5 12.233823915069161 38.14964261867608 35.46221503884418 14.154318427410582 6 30.466149975089163 37.57618844913714 18.34488360722854 13.612777968545158 7 26.66667441614464 33.07440777520625 22.859999093311075 17.398918715338038 8 25.854417350196673 36.08136380364832 20.53358931658934 17.530629529565672 9 26.793972050254766 15.09535465851173 20.44451956275255 37.66615372848095 10-11 24.574977943048314 26.222257353189043 29.354739716345858 19.848024987416785 12-13 25.04425920608923 24.166967927622977 29.15138566478316 21.637387201504637 14-15 22.37488564676562 25.537846706801215 26.902146179178004 25.18512146725516 16-17 21.947829184076003 28.34959031295422 27.51873140692232 22.183849096047457 18-19 21.7657046866665 27.37621560081738 29.51479191443463 21.343287798081494 20-21 22.528121587461083 26.725580781138802 29.46011062409314 21.286187007306975 22-23 22.301908812667605 26.550246259036374 29.33857043054937 21.809274497746646 24-25 22.004147986545664 26.589716657320793 29.454900817508122 21.95123453862542 26-27 21.966662164213684 26.677487277858 29.42390997821851 21.931940579709806 28-29 22.009491734110085 27.03766737733973 29.33720485669346 21.615636031856724 30-31 22.474417588191464 26.718360400763025 29.409491792588042 21.397730218457465 32-33 22.22999905027376 26.793974155589336 29.355689214701947 21.62033757943495 34-35 22.2943148501475 27.022843385169114 29.2403978955075 21.442443869175886 36-37 22.27929910247184 27.02577945085844 29.141598258299474 21.55332318837025 38-39 22.225520967679877 27.1668776300924 29.009728108848993 21.597873293378733 40-41 22.318683964293566 26.939388827981116 29.12125756734365 21.62066964038167 42-43 22.1513096106235 27.07688798725247 28.867605805394636 21.904196596729395 44-45 22.050319096339905 27.268505338305825 28.860110150110536 21.821065415243737 46-47 21.970290288316704 27.026065602795036 28.713280926768718 22.290363182119535 48-49 22.0858631819431 27.18237369977368 28.661256894355752 22.070506223927467 50-51 22.17787344011839 27.43747831176765 28.470538588923645 21.914109659190316 52-53 22.135123658119543 27.493915351634378 28.277058449009562 22.09390254123652 54-55 22.019335959772725 27.344426128108264 28.533751908306883 22.10248600381214 56-57 22.192303319703985 27.393352687365578 28.21947165677382 22.194872336156614 58-59 22.090900552121397 27.423540440078973 28.39546055219579 22.09009845560384 60-61 22.362251792358865 27.4984329356927 28.33917223664252 21.800143035305915 62-63 22.21058074981003 27.597881376031342 28.296927767376406 21.89461010678222 64-65 22.041406051836148 27.302324673051075 28.326932416877405 22.329336858235376 66-67 22.00912900146562 27.33226014922196 28.228291766727892 22.43031908258453 68-69 21.841198610727623 27.4135642974438 28.364070742446923 22.381166349381658 70-71 21.939169275205096 27.23452456749722 28.393170164977583 22.433135992320103 72-73 21.91171107641397 27.613570853376512 27.98731884121151 22.487399228998008 74-75 21.912965219427125 27.62408505673851 27.948655533554064 22.5142941902803 76-77 21.8114427467428 27.42216198119612 28.109882266509196 22.656513005551886 78-79 21.717569436064693 27.42149985480266 28.142464513840014 22.71846619529263 80-81 21.74339575703291 27.55280459089992 27.90816572669777 22.795633925369398 82-83 21.896489924916892 27.533309329123412 27.917473572794897 22.652727173164795 84-85 21.794586502670334 27.705690668988215 27.780006157303287 22.719716671038164 86-87 21.619855589203816 27.61546620047433 27.900116059753916 22.864562150567938 88-89 21.4994599358434 27.42539964166249 27.973707188724813 23.1014332337693 90-91 21.864893520912297 27.308670816217784 27.911197916333595 22.91523774653632 92-93 21.66199152043244 27.435160471879875 27.851557217312962 23.051290790374722 94-95 21.501106828099058 27.33468862988217 27.799665742100448 23.364538799918318 96-97 21.391844244783112 27.233815950492257 27.924366401474305 23.449973403250326 98-99 21.333919919787714 27.19064230286649 27.80458160605015 23.670856171295647 100 20.5807848280224 27.6543054857972 28.01825808731886 23.746651598861543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2552.0 1 1874.5 2 2631.5 3 3668.0 4 2399.5 5 1756.0 6 2489.0 7 4588.0 8 7087.5 9 8104.5 10 6850.0 11 5248.0 12 5187.5 13 5516.0 14 5790.0 15 6104.0 16 6526.5 17 6928.5 18 7424.5 19 7970.0 20 8728.5 21 9930.5 22 11628.5 23 14288.0 24 17615.5 25 21805.0 26 27148.5 27 33018.0 28 38972.0 29 45610.5 30 53401.0 31 61028.0 32 69014.0 33 77532.5 34 85583.0 35 93350.0 36 103186.5 37 112841.5 38 120079.5 39 126642.5 40 130949.0 41 134491.5 42 138322.0 43 142536.5 44 146698.0 45 151310.5 46 155316.0 47 157934.0 48 161491.5 49 164333.0 50 165900.0 51 162735.0 52 155071.5 53 147035.0 54 138793.0 55 128367.0 56 115880.0 57 103819.5 58 91657.0 59 78154.5 60 63993.5 61 52096.0 62 42297.0 63 33073.5 64 25132.5 65 19087.0 66 14365.0 67 10986.5 68 8689.5 69 6826.5 70 5386.5 71 4227.0 72 3268.0 73 2622.0 74 2048.5 75 1539.5 76 1226.0 77 865.5 78 596.0 79 426.5 80 291.0 81 195.0 82 127.5 83 104.0 84 85.5 85 61.0 86 39.5 87 20.0 88 15.0 89 10.5 90 6.5 91 6.5 92 3.5 93 2.0 94 4.0 95 3.5 96 4.0 97 3.5 98 2.5 99 5.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0029990479766306742 2 0.0692105878017792 3 6.277077160389784E-4 4 7.671983196031958E-4 5 0.0017901294124074566 6 5.579624142568695E-4 7 0.0 8 9.764342249495219E-4 9 4.649686785473914E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.671983196031958E-4 18-19 0.0010578037436953154 20-21 0.003068793278412783 22-23 0.0 24-25 2.324843392736957E-5 26-27 1.3949060356421738E-4 28-29 0.001011306875840576 30-31 0.0016273903749158699 32-33 0.002917678457884881 34-35 0.004312584493527055 36-37 0.0011972943472595328 38-39 0.0015460208561700762 40-41 0.0054750061898955335 42-43 0.006893160659465077 44-45 0.005451757755968163 46-47 0.007288384036230359 48-49 0.008904150194182545 50-51 0.008311315129034621 52-53 0.003987106418543881 54-55 0.0024294613454101197 56-57 0.0025108308641559134 58-59 0.003185035448049631 60-61 0.0076487347621045886 62-63 0.01508823361886285 64-65 0.0036732525605243918 66-67 0.010612910087844207 68-69 0.012844759744871686 70-71 0.006172459207716621 72-73 0.007869594884414598 74-75 0.003301277617686479 76-77 0.002906054240921196 78-79 0.007485995724613 80-81 0.005753987397023968 82-83 0.01698298098394347 84-85 0.018157026897275632 86-87 0.02310894332380535 88-89 0.02208601223100109 90-91 0.02465496417997543 92-93 0.03486102667409067 94-95 0.03931310177118194 96-97 0.02388776586037223 98-99 0.018064033161566153 100 0.018714989311532503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4301365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.97531366469774 #Duplication Level Percentage of deduplicated Percentage of total 1 77.71396560383421 51.27203256840494 2 14.586702317012834 19.2472452139699 3 3.9382321904379745 7.79478312145665 4 1.5067925695583304 3.9764444961698695 5 0.7441609194840998 2.454812503998668 6 0.3837590004734939 1.5191172256733791 7 0.2569233203386907 1.1865417652984516 8 0.16142652649144498 0.8520132575260578 9 0.12011638559402871 0.7132244594252243 >10 0.5469248956513618 6.189824323545122 >50 0.027993383327013406 1.25212813088383 >100 0.011903107120077127 1.420254006615362 >500 5.498903549065485E-4 0.2415077647925518 >1k 3.8069329942076545E-4 0.5085783796800193 >5k 4.229925549119615E-5 0.14944234104403545 >10k+ 1.2689776647358843E-4 1.2220504415160247 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17165 0.3990593683632986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15491 0.36014148996888196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11134 0.2588480633473328 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5355 0.12449536368106402 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.3248433927369566E-5 0.0 0.0 0.0 6 0.0 2.3248433927369566E-5 0.0 0.0 2.3248433927369566E-5 7 0.0 2.3248433927369566E-5 0.0 0.0 2.3248433927369566E-5 8 0.0 2.3248433927369566E-5 0.0 0.0 2.3248433927369566E-5 9 0.0 2.3248433927369566E-5 0.0 0.0 2.3248433927369566E-5 10-11 0.0 2.3248433927369566E-5 0.0 0.0 2.3248433927369566E-5 12-13 0.0 3.487265089105435E-5 0.0 0.0 2.3248433927369566E-5 14-15 0.0 4.649686785473913E-5 0.0 0.0 3.487265089105435E-5 16-17 0.0 4.649686785473913E-5 0.0 0.0 4.649686785473913E-5 18-19 0.0 4.649686785473913E-5 0.0 0.0 4.649686785473913E-5 20-21 0.0 4.649686785473913E-5 0.0 0.0 4.649686785473913E-5 22-23 0.0 4.649686785473913E-5 0.0 1.1624216963684783E-4 4.649686785473913E-5 24-25 0.0 1.1624216963684784E-4 0.0 3.7197494283791306E-4 4.649686785473913E-5 26-27 0.0 1.1624216963684784E-4 0.0 6.044592821116087E-4 4.649686785473913E-5 28-29 0.0 1.1624216963684784E-4 0.0 0.001673887242770609 4.649686785473913E-5 30-31 0.0 1.394906035642174E-4 0.0 0.0061143381228981955 4.649686785473913E-5 32-33 0.0 1.394906035642174E-4 0.0 0.014844125062625469 4.649686785473913E-5 34-35 0.0 1.394906035642174E-4 0.0 0.029223281446703546 4.649686785473913E-5 36-37 0.0 1.394906035642174E-4 0.0 0.05104193668753988 4.649686785473913E-5 38-39 0.0 1.394906035642174E-4 0.0 0.08826267940525856 4.649686785473913E-5 40-41 0.0 1.5111482052790218E-4 0.0 0.13516639485372667 4.649686785473913E-5 42-43 0.0 1.6273903749158697E-4 0.0 0.17926867401394675 4.649686785473913E-5 44-45 0.0 1.6273903749158697E-4 0.0 0.2299386357586487 4.649686785473913E-5 46-47 0.0 1.6273903749158697E-4 0.0 0.29531323196241194 4.649686785473913E-5 48-49 0.0 1.6273903749158697E-4 0.0 0.3663255733935623 4.649686785473913E-5 50-51 0.0 1.6273903749158697E-4 0.0 0.44433569343685086 4.649686785473913E-5 52-53 0.0 1.7436325445527176E-4 0.0 0.5251472497683876 4.649686785473913E-5 54-55 0.0 2.4410855623738047E-4 0.0 0.6203844593518569 6.97453017821087E-5 56-57 0.0 2.789812071284348E-4 0.0 0.7266065539660085 9.299373570947827E-5 58-59 0.0 2.789812071284348E-4 0.0 0.8494396546212655 1.0461795267316306E-4 60-61 2.3248433927369566E-5 3.0222964105580436E-4 0.0 0.9818743584885263 1.1624216963684784E-4 62-63 2.3248433927369566E-5 3.0222964105580436E-4 0.0 1.1224808868812575 1.1624216963684784E-4 64-65 2.3248433927369566E-5 3.0222964105580436E-4 0.0 1.261192667908908 1.1624216963684784E-4 66-67 2.3248433927369566E-5 3.2547807498317394E-4 0.0 1.4243501772111875 1.394906035642174E-4 68-69 2.3248433927369566E-5 3.2547807498317394E-4 0.0 1.6194975315975277 1.394906035642174E-4 70-71 2.3248433927369566E-5 3.603507258742283E-4 0.0 1.834661787595333 1.394906035642174E-4 72-73 2.3248433927369566E-5 3.9522337676528265E-4 0.0 2.0477220603227115 1.394906035642174E-4 74-75 2.3248433927369566E-5 3.9522337676528265E-4 0.0 2.257457806998476 1.394906035642174E-4 76-77 2.3248433927369566E-5 3.9522337676528265E-4 0.0 2.4934642840121684 1.394906035642174E-4 78-79 2.3248433927369566E-5 3.9522337676528265E-4 0.0 2.767354084110509 1.394906035642174E-4 80-81 2.3248433927369566E-5 4.1847181069265224E-4 0.0 3.057738648080319 1.394906035642174E-4 82-83 2.3248433927369566E-5 4.1847181069265224E-4 0.0 3.360967971795 1.394906035642174E-4 84-85 2.3248433927369566E-5 4.1847181069265224E-4 0.0 3.668707491691591 1.394906035642174E-4 86-87 2.3248433927369566E-5 4.1847181069265224E-4 0.0 3.9911400218302795 1.394906035642174E-4 88 2.3248433927369566E-5 4.1847181069265224E-4 0.0 4.251441112298073 1.394906035642174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8260 0.0 45.54208 1 TACCTGG 4070 0.0 39.16409 2 GTATCAA 13980 0.0 36.99887 1 GTACCTG 4975 0.0 32.986427 1 ACCTGGG 4945 0.0 32.21391 3 TAACGCA 545 0.0 29.315172 4 TCAACGC 17355 0.0 29.269218 4 ATCAACG 17405 0.0 29.050142 3 CAACGCA 17700 0.0 28.725264 5 TATCAAC 18095 0.0 27.933966 2 AACGCAG 18415 0.0 27.763052 6 GTACATG 14750 0.0 27.735128 1 TATAACG 450 0.0 27.167118 2 TACATGG 15020 0.0 26.577888 2 CATGGGG 9620 0.0 25.93758 4 ATGGGGG 6425 0.0 25.671072 5 ACATGGG 15215 0.0 25.541334 3 ACGCAGA 20735 0.0 24.49806 7 CGCAGAG 21230 0.0 23.816193 8 TGGGGGG 5840 0.0 23.173397 6 >>END_MODULE