FastQCFastQC Report
Mon 6 Jun 2016
ERR1211550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509607
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.1473684623641355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.13029648336855654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.1118508968675862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT250.00524142756.380816
GGTATCA6000.045.4848251
GTATCAA9650.034.1319051
GTACCTG4100.030.98631
TCAACGC10250.030.7175244
CAACGCA10550.030.2894735
TACCTGG4250.029.8633142
AACGCAG10850.029.4461966
TAATACC1452.6577254E-829.1682114
ATCAACG10800.029.1532063
GTACATG15600.028.0509851
TAAGACG700.00883769426.8532754
ACATGGG16150.026.7701363
TACATGG16500.026.4948942
TATCAAC12300.025.6054762
ACGCAGA12600.025.3564457
CATGGGG9750.024.5810724
CGCAGAG13600.023.1488048
ACCTGGG5400.021.7561243
GAGTACA12200.021.212541
Produced by FastQC (version 0.11.5)