##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 509607 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.052440409962973 31.0 31.0 34.0 28.0 34.0 2 31.09876237963764 31.0 31.0 34.0 27.0 34.0 3 31.21260304509161 31.0 31.0 34.0 27.0 34.0 4 34.69552419805851 37.0 35.0 37.0 32.0 37.0 5 34.776582739248084 37.0 35.0 37.0 32.0 37.0 6 34.889187157947205 37.0 35.0 37.0 32.0 37.0 7 34.803933226976866 37.0 35.0 37.0 32.0 37.0 8 34.76249541313208 37.0 35.0 37.0 31.0 37.0 9 36.31968948621192 39.0 37.0 39.0 31.0 39.0 10-11 36.29884401116939 39.0 37.0 39.0 31.0 39.0 12-13 36.24067173331606 39.0 37.0 39.0 31.0 39.0 14-15 37.41110306569572 40.0 37.0 41.0 31.0 41.0 16-17 37.391277984800055 40.0 37.0 41.0 31.0 41.0 18-19 37.38692266785974 40.0 37.0 41.0 31.0 41.0 20-21 37.32517704819596 40.0 37.0 41.0 31.0 41.0 22-23 37.15965636264808 40.0 37.0 41.0 30.0 41.0 24-25 37.09584542598512 40.0 37.0 41.0 30.0 41.0 26-27 36.9618127302019 40.0 36.0 41.0 30.0 41.0 28-29 36.808885082033804 40.0 36.0 41.0 30.0 41.0 30-31 36.654698620701836 39.0 36.0 41.0 29.0 41.0 32-33 36.51702194043645 39.0 36.0 41.0 29.0 41.0 34-35 36.35509323851517 39.0 35.0 41.0 28.5 41.0 36-37 36.201925209033625 39.0 35.0 41.0 28.0 41.0 38-39 36.08465543055728 39.0 35.0 40.0 27.0 41.0 40-41 36.04308614285126 39.0 35.0 40.0 27.0 41.0 42-43 36.00244305906316 39.0 35.0 40.0 27.0 41.0 44-45 35.84353531250552 39.0 35.0 40.0 27.0 41.0 46-47 35.842272574748776 39.0 35.0 40.0 27.0 41.0 48-49 35.806420437709846 39.0 35.0 40.5 26.0 41.0 50-51 34.88097592850961 38.0 34.0 40.0 25.0 40.5 52-53 35.10722282072263 38.0 34.0 39.5 25.5 40.5 54-55 35.69482267708254 39.0 35.0 40.5 26.0 41.0 56-57 35.67058341035347 39.0 34.5 41.0 26.0 41.0 58-59 35.52573551776173 39.0 34.0 40.5 26.0 41.0 60-61 35.34467933132787 38.0 34.0 40.0 26.0 41.0 62-63 35.099316728380884 38.0 34.0 40.0 26.0 41.0 64-65 34.83051056990975 37.5 34.0 40.0 25.5 41.0 66-67 34.5486168753569 37.0 33.0 40.0 25.0 41.0 68-69 34.280826205291525 36.5 33.0 39.5 25.0 41.0 70-71 33.92313488629473 36.0 33.0 39.0 24.0 41.0 72-73 33.584428785318885 35.5 32.5 39.0 24.0 40.5 74-75 33.208305615896165 35.0 32.0 38.0 23.5 40.0 76-77 32.86013732150461 35.0 32.0 37.0 23.0 39.0 78-79 32.52721999501577 35.0 32.0 37.0 23.0 39.0 80-81 32.18270942118142 35.0 31.0 36.0 22.0 38.5 82-83 31.86532563328212 35.0 31.0 36.0 21.5 37.0 84-85 31.607906681030677 35.0 31.0 35.5 21.0 37.0 86-87 31.315114392070754 34.0 31.0 35.0 20.0 36.5 88-89 31.0971209186687 34.0 30.5 35.0 20.0 36.0 90-91 30.870003747986196 34.0 30.0 35.0 20.0 36.0 92-93 30.71611948030541 34.0 30.0 35.0 19.5 36.0 94-95 30.566940799478814 34.0 30.0 35.0 19.0 35.0 96-97 30.48753156844392 34.0 30.0 35.0 19.0 35.0 98-99 30.31717382218062 34.0 29.0 35.0 18.0 35.0 100 28.966056196245344 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6912548181895382 1101 2 0.7165599618840339 1101 3 0.5867027261814606 1101 4 0.5491137980943961 1101 5 0.6557597207436174 1101 6 0.6690828310126378 1101 7 0.7621880112180932 1101 8 0.8458323705127526 1101 9 0.8141974750885694 1101 10-11 0.7989373983634209 1101 12-13 0.760049530395321 1101 14-15 0.7601877874771574 1101 16-17 0.8021301698751486 1101 18-19 0.8514025199238731 1101 20-21 0.9837113066577174 1101 22-23 0.9443892772113429 1101 24-25 0.7539478797377868 1101 26-27 0.8399982723679926 1101 28-29 0.8947849856458632 1101 30-31 0.8202670311258444 1101 32-33 0.8081967341140412 1101 34-35 0.7905284767293637 1101 36-37 0.9420208603512634 1101 38-39 1.1138632342872796 1101 40-41 0.756961143324844 1101 42-43 0.6638273290236825 1101 44-45 0.9161203821542045 1101 46-47 0.9012832670879902 1101 48-49 0.9296739233514586 1101 50-51 0.9691960287484989 1101 52-53 1.0023750724099472 1101 54-55 0.9450632514686532 1101 56-57 1.1080440103984657 1101 58-59 0.7942200549818352 1101 60-61 0.8145531052560031 1101 62-63 0.971774533470402 1101 64-65 0.930010284223286 1101 66-67 0.8246011902224382 1101 68-69 0.6621172751912567 1101 70-71 0.664880821353627 1101 72-73 0.633187827183562 1101 74-75 0.7374045476873405 1101 76-77 0.765694231513578 1101 78-79 0.6658303503400518 1101 80-81 0.7284482528941183 1101 82-83 0.7299091226960819 1101 84-85 0.5361718142422198 1101 86-87 0.6079480104513593 1101 88-89 0.8476168962106776 1101 90-91 0.5593035459750197 1101 92-93 0.34149453044876665 1101 94-95 0.3349049779049338 1101 96-97 0.22498978539923797 1101 98-99 0.14551332381976678 1101 100 -0.04360074613152776 1102 1 0.34575888323018944 1102 2 0.38636483993281345 1102 3 0.13416614081560496 1102 4 0.17913005825699457 1102 5 0.24988980204442868 1102 6 0.18631860337035988 1102 7 0.1840579299172802 1102 8 0.25438521604120723 1102 9 -0.002761874504926709 1102 10-11 -0.12010325204308003 1102 12-13 0.17605766047662286 1102 14-15 0.05581380373732259 1102 16-17 0.1203578121515747 1102 18-19 0.18583341423281752 1102 20-21 -0.0951423518788701 1102 22-23 -0.059952186203290125 1102 24-25 0.3103381236402214 1102 26-27 0.055949491880191715 1102 28-29 0.07689880678407235 1102 30-31 -0.14770044854895303 1102 32-33 0.15470079915468915 1102 34-35 0.05635774502204072 1102 36-37 -0.0730604404617381 1102 38-39 -0.02519367628182323 1102 40-41 -0.11038845017108656 1102 42-43 -0.1665547847974551 1102 44-45 -0.18610726012222045 1102 46-47 -0.08358665161119205 1102 48-49 -0.08007404412821728 1102 50-51 -0.02555193873117645 1102 52-53 -0.11908021214290443 1102 54-55 0.2333559343954832 1102 56-57 0.10909279088627244 1102 58-59 0.18874038018508088 1102 60-61 0.07176448736982621 1102 62-63 -0.03307912506618038 1102 64-65 -0.035892154801111076 1102 66-67 -0.19706547644423722 1102 68-69 -0.1333217491989913 1102 70-71 -0.17175332498784002 1102 72-73 -0.3588365630603363 1102 74-75 -0.054018216540306696 1102 76-77 -0.2746065814945524 1102 78-79 -0.31966558461929395 1102 80-81 0.04047264313802401 1102 82-83 -0.40729412933643516 1102 84-85 -0.19805582803420307 1102 86-87 -0.5049706887356358 1102 88-89 -0.5398139980982677 1102 90-91 -0.21146881174855636 1102 92-93 -0.37474124190896063 1102 94-95 -0.629208843233279 1102 96-97 -0.5323923284218992 1102 98-99 -0.6809419607330831 1102 100 -0.472999120115265 1103 1 0.4221445520298417 1103 2 0.3700428516178711 1103 3 0.21285481743621304 1103 4 0.09208718212481415 1103 5 0.03643652682726639 1103 6 -0.04871184579344856 1103 7 0.23591424695953833 1103 8 0.17040271271807939 1103 9 0.26204538383382214 1103 10-11 0.38885374817330387 1103 12-13 0.22474154560445214 1103 14-15 0.6761459623821011 1103 16-17 0.4844571660728647 1103 18-19 0.34450137923946045 1103 20-21 0.3776504701558139 1103 22-23 0.5379101897588683 1103 24-25 0.3377539633879749 1103 26-27 0.2937891468927063 1103 28-29 0.33737053697665687 1103 30-31 0.34171189804600033 1103 32-33 0.1369800040760154 1103 34-35 0.20652467444799072 1103 36-37 0.2889600238493628 1103 38-39 0.2808822456941229 1103 40-41 0.26768737906628814 1103 42-43 0.14558778529744387 1103 44-45 0.4141469981237904 1103 46-47 0.25619201233514133 1103 48-49 0.14252943665943718 1103 50-51 0.3866903253264482 1103 52-53 0.5595651864783804 1103 54-55 0.18416971534698234 1103 56-57 0.34178545526538784 1103 58-59 0.5492808914837326 1103 60-61 0.46207021552216077 1103 62-63 0.5632878414551996 1103 64-65 0.6015464058962863 1103 66-67 0.590323623682508 1103 68-69 0.579003206750194 1103 70-71 0.368831391695835 1103 72-73 0.3192562682482034 1103 74-75 0.27040834996871155 1103 76-77 0.2263710375972252 1103 78-79 0.30900145300164894 1103 80-81 0.1793950209549635 1103 82-83 0.2667684382854354 1103 84-85 0.17881439979354852 1103 86-87 0.17530922718139408 1103 88-89 0.3095978848038321 1103 90-91 0.2804822531993558 1103 92-93 0.08985194489743265 1103 94-95 0.13978710718249943 1103 96-97 0.025472675133080713 1103 98-99 -0.09307602979240315 1103 100 -0.4730532176144173 1104 1 0.5312402462405394 1104 2 0.33207544457984284 1104 3 0.30818542199202525 1104 4 0.337722176965066 1104 5 0.08693276263797145 1104 6 0.2821247253660033 1104 7 0.3943738035678166 1104 8 0.4420545927349764 1104 9 0.3270062182579707 1104 10-11 0.4636077080173351 1104 12-13 0.5385959784827534 1104 14-15 0.5782770406647728 1104 16-17 0.4003013902758781 1104 18-19 0.40969942338470844 1104 20-21 0.6420683194081676 1104 22-23 0.544950297247766 1104 24-25 0.5694178250929767 1104 26-27 0.42420774413483997 1104 28-29 0.5253860785420343 1104 30-31 0.32294462675425706 1104 32-33 0.25775957564409424 1104 34-35 0.19145925997162294 1104 36-37 0.5556547401326881 1104 38-39 0.5005626878391922 1104 40-41 0.390172436585317 1104 42-43 0.3471952707358881 1104 44-45 0.3515721125731446 1104 46-47 0.5034563089823507 1104 48-49 0.4425391328232209 1104 50-51 0.6270703456883879 1104 52-53 0.8693022126649552 1104 54-55 0.7644712660406086 1104 56-57 0.9125103127664147 1104 58-59 0.8964295267486762 1104 60-61 0.7891997354927938 1104 62-63 0.5606889232700425 1104 64-65 0.5386113771194587 1104 66-67 0.5133170372169644 1104 68-69 0.2973504263752247 1104 70-71 0.49584074849388315 1104 72-73 0.7247688836498725 1104 74-75 0.7590621068858852 1104 76-77 0.7026414081984598 1104 78-79 0.21508718094114698 1104 80-81 0.39334260626388584 1104 82-83 0.6287470097634831 1104 84-85 0.5632118871019678 1104 86-87 0.3137185022546376 1104 88-89 0.3226132532234267 1104 90-91 0.45895746218631217 1104 92-93 0.3912468073157953 1104 94-95 0.26975379393407906 1104 96-97 0.3244815886779264 1104 98-99 0.062221884839804176 1104 100 -0.007640818991269782 1105 1 0.26614409762714786 1105 2 0.6279096982636467 1105 3 0.4218767156366461 1105 4 0.43140905292743525 1105 5 0.46847325325679634 1105 6 0.6345661350548113 1105 7 0.47162562105992123 1105 8 0.5713262193703983 1105 9 0.7138319214857631 1105 10-11 0.6466069941455004 1105 12-13 0.7406857342617528 1105 14-15 0.7057255730659122 1105 16-17 0.4954096074849943 1105 18-19 0.47513714206798596 1105 20-21 0.7880663154450644 1105 22-23 0.7950887499705601 1105 24-25 0.9302800414249575 1105 26-27 0.71189809662107 1105 28-29 0.8520784830096559 1105 30-31 0.853781969614424 1105 32-33 0.8426607059945326 1105 34-35 0.8755741709297098 1105 36-37 0.6260015281895832 1105 38-39 0.7550547984349052 1105 40-41 0.5003231819891667 1105 42-43 0.5509784713786914 1105 44-45 0.38853338742661236 1105 46-47 0.26812509485601055 1105 48-49 0.6876756808207034 1105 50-51 0.7542803872722246 1105 52-53 0.9137687455206631 1105 54-55 0.9472688753702343 1105 56-57 0.7558682634740421 1105 58-59 1.0347349934704084 1105 60-61 0.7723053020925548 1105 62-63 1.1097956231013413 1105 64-65 0.8222457851020195 1105 66-67 0.5891090988340295 1105 68-69 0.6197323894267583 1105 70-71 0.6864221218808737 1105 72-73 0.5439400240201167 1105 74-75 0.6294783613956056 1105 76-77 0.9488295566453786 1105 78-79 0.5343751658057911 1105 80-81 0.5693744391858573 1105 82-83 0.6942711613604082 1105 84-85 0.6257060848924816 1105 86-87 0.5105982740717465 1105 88-89 0.43076803856568446 1105 90-91 0.6361225266428576 1105 92-93 0.7615894337879574 1105 94-95 0.7225886334409601 1105 96-97 0.6357121052586407 1105 98-99 0.4694377526422606 1105 100 0.592430888314869 1106 1 0.3589840251527896 1106 2 0.38405319205811494 1106 3 0.21243193314471043 1106 4 0.5158797555411994 1106 5 0.49707886967978965 1106 6 0.5056012062544184 1106 7 0.6447992297867557 1106 8 0.5970083859866406 1106 9 0.5429324847597528 1106 10-11 0.6330418471061705 1106 12-13 0.4982042692734652 1106 14-15 0.5472728938601392 1106 16-17 0.34404119502021757 1106 18-19 0.5935011659586849 1106 20-21 0.5528679797718326 1106 22-23 0.6468341514860043 1106 24-25 0.6391877249989051 1106 26-27 0.41586481008559417 1106 28-29 0.6154813105975094 1106 30-31 0.7100349228086245 1106 32-33 0.7641541809225529 1106 34-35 1.1082518809846817 1106 36-37 0.7722046127690589 1106 38-39 0.6818206810957932 1106 40-41 0.6074234257232973 1106 42-43 0.6531445437238759 1106 44-45 0.5145323744172643 1106 46-47 0.6989022226005659 1106 48-49 0.751490170933657 1106 50-51 0.7728884852281865 1106 52-53 0.6221410298567491 1106 54-55 0.5866783385092802 1106 56-57 0.6316726370909223 1106 58-59 0.7149724727768003 1106 60-61 0.7367445946177043 1106 62-63 0.7072909744762086 1106 64-65 0.9381843654611899 1106 66-67 0.9270479987330731 1106 68-69 0.8029664047850673 1106 70-71 0.7523102217404798 1106 72-73 0.7211452739920787 1106 74-75 0.7835747604532983 1106 76-77 0.6768586415715987 1106 78-79 0.6534241607849296 1106 80-81 0.6783341329714929 1106 82-83 0.7162650221158167 1106 84-85 0.7651563403544053 1106 86-87 0.6093870820180882 1106 88-89 0.6306130277387219 1106 90-91 0.6879882655108034 1106 92-93 0.5760438534661709 1106 94-95 0.9303711287753416 1106 96-97 0.8315526480684845 1106 98-99 0.5542347938391039 1106 100 0.5824843602514527 1107 1 0.3137866533955318 1107 2 0.2674738195619355 1107 3 0.23277763254850115 1107 4 0.4042373936000132 1107 5 0.2398870615675861 1107 6 0.20833376734222497 1107 7 0.3682179737649065 1107 8 0.24417693980114308 1107 9 0.2983023440024297 1107 10-11 0.4168475107229739 1107 12-13 0.39348024200580767 1107 14-15 0.22712786238352578 1107 16-17 0.3729841024594194 1107 18-19 0.3239306098115122 1107 20-21 0.5558236662082834 1107 22-23 0.44125819106901787 1107 24-25 0.5654422617602606 1107 26-27 0.6432679352893445 1107 28-29 0.673249405121517 1107 30-31 0.633038566706368 1107 32-33 0.4953877453499942 1107 34-35 0.49750600481924323 1107 36-37 0.5588935195272953 1107 38-39 0.6415186649988911 1107 40-41 0.7501783717892607 1107 42-43 0.8884602878251755 1107 44-45 0.8847683222291138 1107 46-47 0.6404930049157116 1107 48-49 0.4994454589319801 1107 50-51 0.56099003623914 1107 52-53 0.7604986679155559 1107 54-55 0.7370669967870072 1107 56-57 0.8719616133947241 1107 58-59 0.5808455231466212 1107 60-61 0.5674482363421447 1107 62-63 0.6300591776651601 1107 64-65 0.8456507336614862 1107 66-67 0.8853315273010907 1107 68-69 0.8608775748167261 1107 70-71 1.1291167764098091 1107 72-73 0.9589331455356245 1107 74-75 0.84740454768734 1107 76-77 0.7594095873188138 1107 78-79 0.8955531967820036 1107 80-81 1.0855306498978692 1107 82-83 0.8551750402990841 1107 84-85 0.9383628254781762 1107 86-87 0.9384498831105361 1107 88-89 0.8993359973342692 1107 90-91 0.6258953062746464 1107 92-93 0.6935993993626219 1107 94-95 0.6751896220996869 1107 96-97 0.641068437084634 1107 98-99 0.6869327994751977 1107 100 0.8559722875763391 1108 1 0.21896231361915497 1108 2 0.15815776809975546 1108 3 0.2150061996732333 1108 4 0.24278473045271198 1108 5 0.31122955620979553 1108 6 0.18403712717351084 1108 7 0.39694121048682973 1108 8 0.23262944546705455 1108 9 0.19408047326042066 1108 10-11 0.06879480238536217 1108 12-13 0.013186641912689367 1108 14-15 0.34884043829982403 1108 16-17 0.3474464404418782 1108 18-19 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 0 2.0 1 2.0 2 9.0 3 8.0 4 8.0 5 10.0 6 15.0 7 156.0 8 344.0 9 482.0 10 751.0 11 1169.0 12 1611.0 13 1875.0 14 2044.0 15 2127.0 16 2359.0 17 2488.0 18 2794.0 19 3118.0 20 3515.0 21 3941.0 22 4441.0 23 4682.0 24 5037.0 25 5367.0 26 5939.0 27 6672.0 28 7807.0 29 8969.0 30 10824.0 31 13393.0 32 16509.0 33 21268.0 34 28938.0 35 41010.0 36 61944.0 37 100474.0 38 111198.0 39 26268.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76666155726655 19.867705840044334 13.036044852672413 24.329587750016703 2 14.95999890004341 20.19756006057618 40.11895244848373 24.72348859089668 3 17.982122106717753 26.10981124964668 30.25835400898213 25.649712634653433 4 12.075815392346225 16.34749768376753 39.042846374899895 32.533840548986355 5 12.608274922615204 38.13043828157663 35.72792742218829 13.53335937361988 6 31.216628041373866 37.31399517658071 18.278168827524492 13.191207954520925 7 26.976483147302154 32.25737452707264 23.018807202624846 17.74733512300036 8 25.378005153233136 36.010620448484346 20.837822297839605 17.77355210044291 9 26.484151778705765 14.764003579390247 20.159777387045118 38.59206725485886 10-11 24.397985796173717 25.847117533817 29.818910045704218 19.935986624305066 12-13 24.824437547649865 23.699778149110095 29.191593411391615 22.284190891848425 14-15 22.28294308539399 25.194666028165365 26.520308560987505 26.00208232545314 16-17 22.22282185810151 28.083172331099743 27.150716733864044 22.5432890769347 18-19 21.9425219342791 27.619086870637556 28.58340030635866 21.854990888724686 20-21 22.736555145907932 27.01580812397636 28.393977427620104 21.8536593024956 22-23 22.219364869348084 26.966747610456864 28.22762545469536 22.58626206549969 24-25 22.075255996977592 26.945159976645026 28.39621021436527 22.58337381201211 26-27 22.044924680707428 27.214049658139118 28.216867753608966 22.524157907544488 28-29 22.086387488505515 27.274190430112068 28.20081513925377 22.438606942128647 30-31 22.57049046487301 27.095775184137754 28.315019321462426 22.018715029526806 32-33 22.299266563170363 27.047175865865654 28.215925215742693 22.437632355221297 34-35 22.397501106464997 27.312560904641852 28.24778142728587 22.042156561607285 36-37 22.320463892587945 27.22660493469764 28.119710037692748 22.33322113502167 38-39 22.27359975506263 27.358110999022607 28.10411015901303 22.264179086901738 40-41 22.390069314208382 27.07169795766956 28.243847051422836 22.294385676699218 42-43 22.16182899228367 27.118555901687934 28.14095505055561 22.578660055472785 44-45 22.082482726130653 27.342375942211056 28.050800879396988 22.524340452261306 46-47 22.12907227097248 27.17810929259901 27.908218239770132 22.784600196658378 48-49 22.11889545352405 27.25550168433427 28.046201686394916 22.579401175746764 50-51 22.201659496969885 27.365705560963356 27.86447703080353 22.568157911263228 52-53 22.17113708002779 27.43779170870151 27.833475303141526 22.557595908129176 54-55 22.15707903807049 27.399327936939127 27.992682677888652 22.450910347101726 56-57 22.15881657919632 27.354535569747952 27.800457698241043 22.686190152814685 58-59 22.16988214650576 27.40974526412274 27.979532413391077 22.44084017598042 60-61 22.453680304548502 27.508290388841072 27.79142471031482 22.2466045962956 62-63 22.247886311900164 27.393083653261897 28.01877263263497 22.34025740220297 64-65 22.142278796563012 27.18901969251606 27.996643488416602 22.67205802250433 66-67 22.139897664577695 27.306619614093 27.87717786618672 22.676304855142583 68-69 22.080332265887296 27.421455370908877 27.971633349461158 22.526579013742673 70-71 22.11198413827965 27.16064408792654 27.958834124292675 22.76853764950113 72-73 22.008338960692246 27.43788966188734 27.813221414325422 22.740549963094995 74-75 22.225101316302183 27.348089344832697 27.670613315744685 22.756196023120435 76-77 22.11342285238189 27.234124402233167 27.814889288626805 22.83756345675814 78-79 22.086018403216297 27.189540174729483 27.857606168816197 22.86683525323803 80-81 22.051785302473466 27.37584824560973 27.663282679842787 22.909083772074013 82-83 22.302994856959494 27.382152148036354 27.725512169703652 22.589340825300503 84-85 22.144643836705296 27.426963193826353 27.657474036864073 22.770918932604282 86-87 22.000653583867276 27.460924811358623 27.80636253099862 22.73205907377549 88-89 22.03394820332809 27.259247337891296 27.851481271767177 22.855323187013443 90-91 22.328674492576965 27.240233069623514 27.866089979321302 22.56500245847822 92-93 22.297593706481063 27.184668969029975 27.768012852408308 22.749724472080654 94-95 22.118036133109587 27.222990942188986 27.764398779207273 22.894574145494158 96-97 22.05889279658356 27.20357344721472 27.913140582804747 22.824393173396974 98-99 22.037525882456848 27.038065611414773 27.91281905341354 23.011589452714837 100 22.11523549839127 27.084916049775327 27.774538730175557 23.025309721657845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 81.0 1 59.0 2 84.0 3 126.0 4 96.5 5 87.5 6 125.5 7 224.0 8 325.0 9 363.0 10 307.5 11 228.0 12 220.0 13 225.5 14 261.5 15 301.5 16 310.0 17 338.5 18 371.5 19 382.0 20 440.0 21 528.0 22 648.5 23 835.0 24 1068.0 25 1376.5 26 1839.0 27 2430.5 28 3114.0 29 4096.0 30 5266.5 31 6412.5 32 7730.5 33 9116.5 34 10591.0 35 12082.5 36 13600.0 37 15082.5 38 16520.0 39 17518.0 40 17985.0 41 18405.5 42 18752.0 43 19253.0 44 19634.0 45 20055.5 46 20374.5 47 20521.5 48 20721.0 49 20597.0 50 20315.0 51 19418.5 52 18176.5 53 17299.0 54 16012.0 55 14330.0 56 12768.5 57 11293.0 58 9760.5 59 8161.5 60 6680.0 61 5497.0 62 4436.5 63 3395.5 64 2537.0 65 1901.0 66 1451.0 67 1089.5 68 850.0 69 684.5 70 535.5 71 400.0 72 312.0 73 262.0 74 203.5 75 172.5 76 140.0 77 93.0 78 71.5 79 60.0 80 48.0 81 35.5 82 27.0 83 25.5 84 23.0 85 14.5 86 10.5 87 10.0 88 7.0 89 6.5 90 5.0 91 3.5 92 3.0 93 2.0 94 1.5 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14540616592786207 2 0.09732990323916273 3 0.029630676187728976 4 0.03120051333674773 5 0.02747215010782819 6 0.001962296436273442 7 0.002943444654410163 8 0.0047095114470562605 9 0.004513281803428916 10-11 0.00510197073431095 12-13 0.005985004130633999 14-15 0.015796486312001208 16-17 0.007554841279652753 18-19 0.0144228788066098 20-21 0.01775878274827465 22-23 0.039245928725468844 24-25 0.015600256668373865 26-27 0.008437874675975801 28-29 0.02423436098797701 30-31 0.013736075053914096 32-33 0.01726820863920629 34-35 0.01991730882817544 36-37 0.01815124203552934 38-39 0.017464438282833634 40-41 0.022664523838958256 42-43 0.032966580129393824 44-45 0.032770350485766486 46-47 0.018445586500970355 48-49 0.011479434152199637 50-51 0.010792630399503931 52-53 0.02138903115538052 54-55 0.02570608331518209 56-57 0.019819194006361764 58-59 0.023253212769840288 60-61 0.025804198136995766 62-63 0.04876306644139504 64-65 0.02933633172228796 66-67 0.020800342224498485 68-69 0.02658911671150514 70-71 0.040128962121791886 72-73 0.037479861932822746 74-75 0.03679305818012704 76-77 0.020898457046312158 78-79 0.015011567737491831 80-81 0.01952484954092075 82-83 0.016385175242883243 84-85 0.016973864173765275 86-87 0.021290916333566844 88-89 0.018739930966411373 90-91 0.02855141314777858 92-93 0.025607968493368417 94-95 0.02001542364998911 96-97 0.013932304697541439 98-99 0.018053127213715665 100 0.038853469438214154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 509607.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.60663643579655 #Duplication Level Percentage of deduplicated Percentage of total 1 90.27088373853977 76.37515841204693 2 7.247263974583043 12.263332565035869 3 1.2646347381294731 3.209894745389973 4 0.47158072954239744 1.5959543733808528 5 0.2217166022366678 0.9379347978608933 6 0.12840781039565932 0.6518491757797348 7 0.08515253740108295 0.5043128841435295 8 0.06794435168975152 0.4598834449024565 9 0.03846082767229022 0.29286371375003534 >10 0.19599473423632788 2.7972616585610623 >50 0.005150562275300703 0.270574583257352 >100 0.0021070449736737074 0.24779528337765408 >500 7.02348324557761E-4 0.39318436251363803 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.1473684623641355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 664 0.13029648336855654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 570 0.1118508968675862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.81148218136721E-5 0.0 22-23 0.0 0.0 0.0 4.905741090683606E-4 0.0 24-25 0.0 0.0 0.0 5.886889308820327E-4 0.0 26-27 0.0 0.0 0.0 5.886889308820327E-4 0.0 28-29 0.0 0.0 0.0 7.849185745093768E-4 0.0 30-31 0.0 0.0 0.0 0.0023547557235281307 0.0 32-33 0.0 0.0 0.0 0.007554841279652752 0.0 34-35 0.0 0.0 0.0 0.013245500944845733 0.0 36-37 0.0 0.0 0.0 0.02550985367155475 0.0 38-39 0.0 0.0 0.0 0.040913880696301266 0.0 40-41 0.0 0.0 0.0 0.0625972563171228 0.0 42-43 0.0 1.962296436273442E-4 0.0 0.08477120604701269 0.0 44-45 0.0 1.962296436273442E-4 0.0 0.11018294489675377 0.0 46-47 0.0 1.962296436273442E-4 0.0 0.13844001357909136 0.0 48-49 0.0 1.962296436273442E-4 0.0 0.17278020121387658 0.0 50-51 0.0 1.962296436273442E-4 0.0 0.20466751830332 0.0 52-53 0.0 1.962296436273442E-4 0.0 0.24175492094888806 0.0 54-55 0.0 3.924592872546884E-4 0.0 0.2880651168449413 0.0 56-57 0.0 3.924592872546884E-4 0.0 0.32956768647212464 0.0 58-59 0.0 3.924592872546884E-4 0.0 0.38254969025150753 0.0 60-61 0.0 3.924592872546884E-4 0.0 0.44406768352868 0.0 62-63 0.0 3.924592872546884E-4 0.0 0.515299044165406 0.0 64-65 0.0 3.924592872546884E-4 0.0 0.5874134381984548 0.0 66-67 0.0 3.924592872546884E-4 0.0 0.6707129219182625 0.0 68-69 0.0 3.924592872546884E-4 0.0 0.7628427396013007 0.0 70-71 0.0 3.924592872546884E-4 0.0 0.8798937220250114 0.0 72-73 0.0 3.924592872546884E-4 0.0 0.9947861783688214 1.962296436273442E-4 74-75 0.0 3.924592872546884E-4 0.0 1.102908712007488 1.962296436273442E-4 76-77 0.0 3.924592872546884E-4 0.0 1.2291824876816841 1.962296436273442E-4 78-79 0.0 3.924592872546884E-4 0.0 1.3831246431073356 1.962296436273442E-4 80-81 0.0 3.924592872546884E-4 0.0 1.5403045876528383 1.962296436273442E-4 82-83 0.0 3.924592872546884E-4 0.0 1.7114168368958826 1.962296436273442E-4 84-85 0.0 3.924592872546884E-4 0.0 1.888710319913188 1.962296436273442E-4 86-87 0.0 3.924592872546884E-4 0.0 2.0818002892424947 1.962296436273442E-4 88 0.0 3.924592872546884E-4 0.0 2.236233018777215 1.962296436273442E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 25 0.005241427 56.38081 6 GGTATCA 600 0.0 45.484825 1 GTATCAA 965 0.0 34.131905 1 GTACCTG 410 0.0 30.9863 1 TCAACGC 1025 0.0 30.717524 4 CAACGCA 1055 0.0 30.289473 5 TACCTGG 425 0.0 29.863314 2 AACGCAG 1085 0.0 29.446196 6 TAATACC 145 2.6577254E-8 29.168211 4 ATCAACG 1080 0.0 29.153206 3 GTACATG 1560 0.0 28.050985 1 TAAGACG 70 0.008837694 26.853275 4 ACATGGG 1615 0.0 26.770136 3 TACATGG 1650 0.0 26.494894 2 TATCAAC 1230 0.0 25.605476 2 ACGCAGA 1260 0.0 25.356445 7 CATGGGG 975 0.0 24.581072 4 CGCAGAG 1360 0.0 23.148804 8 ACCTGGG 540 0.0 21.756124 3 GAGTACA 1220 0.0 21.21254 1 >>END_MODULE