FastQCFastQC Report
Mon 6 Jun 2016
ERR1211550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509607
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.20172407364890985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.1979957104199903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.14344386949158863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.040.6505551
GTACCTG4950.032.309271
GTATCAA13300.031.4768921
TACCTGG5350.029.8936272
TACCCGG700.0087962926.879142
CAGTGCG1057.421827E-526.852779
CGGCTAT700.008838505526.852776
ACCGTGA700.008838505526.852778
ACCTGGG5750.026.1522643
TAGACTG2051.0804797E-925.2154065
ATCAACG16800.024.3353233
CATGGGG8750.024.1674924
CAACGCA16750.024.1274155
TCAACGC16750.024.1274154
TATCAAC17400.023.7895872
AACGCAG17200.023.7693846
GTACATG17450.023.7214221
ACATGGG17300.022.8170933
TACATGG17950.022.536552
ATGGGGG5650.021.6247965
Produced by FastQC (version 0.11.5)