##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211550_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 509607 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09377814668951 33.0 31.0 34.0 30.0 34.0 2 32.34332927137971 34.0 31.0 34.0 31.0 34.0 3 32.36577794261068 34.0 31.0 34.0 30.0 34.0 4 35.98692914343798 37.0 35.0 37.0 35.0 37.0 5 35.910576189102585 37.0 35.0 37.0 35.0 37.0 6 35.928727431138114 37.0 35.0 37.0 35.0 37.0 7 35.85382068927625 37.0 35.0 37.0 35.0 37.0 8 35.85121868420175 37.0 35.0 37.0 35.0 37.0 9 37.519506207724774 39.0 37.0 39.0 35.0 39.0 10-11 37.481703548028186 39.0 37.0 39.0 35.0 39.0 12-13 37.394587397739826 39.0 37.0 39.0 34.0 39.0 14-15 38.6393495379773 40.0 38.0 41.0 34.0 41.0 16-17 38.56927102649689 40.0 38.0 41.0 34.0 41.0 18-19 38.54481590715983 40.0 38.0 41.0 34.0 41.0 20-21 38.485084584787884 40.0 38.0 41.0 34.0 41.0 22-23 38.382481009876244 40.0 38.0 41.0 34.0 41.0 24-25 38.27856171520407 40.0 38.0 41.0 34.0 41.0 26-27 38.09837384494326 40.0 38.0 41.0 33.0 41.0 28-29 38.13771396389767 40.0 38.0 41.0 33.5 41.0 30-31 38.07990667318149 40.0 37.5 41.0 33.0 41.0 32-33 37.91683787703073 40.0 37.0 41.0 33.0 41.0 34-35 37.81664400214283 40.0 37.0 41.0 33.0 41.0 36-37 37.767970220189284 40.0 37.0 41.0 33.0 41.0 38-39 37.670186045325124 40.0 37.0 41.0 32.5 41.0 40-41 37.52128404829604 40.0 37.0 41.0 32.0 41.0 42-43 37.38356517865728 40.0 37.0 41.0 31.5 41.0 44-45 37.21865672959751 40.0 36.0 41.0 31.0 41.0 46-47 36.99388352200813 39.0 36.0 41.0 30.5 41.0 48-49 36.807376076074306 39.0 36.0 41.0 30.0 41.0 50-51 36.61752880160594 39.0 35.0 40.5 30.0 41.0 52-53 36.434030537257144 39.0 35.0 40.0 29.5 41.0 54-55 36.24162246593944 39.0 35.0 40.0 29.0 41.0 56-57 36.04748757375782 38.5 35.0 40.0 28.0 41.0 58-59 35.79475262309976 38.0 34.0 40.0 28.0 41.0 60-61 35.47928403652226 38.0 34.0 40.0 27.5 41.0 62-63 35.148196551460245 37.5 34.0 40.0 26.5 41.0 64-65 34.839747099235296 37.0 33.5 40.0 26.0 41.0 66-67 34.50030415594762 36.5 33.0 39.0 26.0 41.0 68-69 34.10468262798588 36.0 32.5 39.0 26.0 40.0 70-71 33.71218017020959 35.5 32.0 39.0 25.5 40.0 72-73 33.29947685177009 35.0 32.0 38.0 25.0 40.0 74-75 32.879871155615994 35.0 31.0 37.0 24.0 39.0 76-77 31.371213503739156 33.5 29.5 35.5 22.5 38.5 78-79 32.14254415657555 34.0 31.0 36.0 23.5 39.0 80-81 32.0878323884876 34.0 31.0 36.0 24.0 37.5 82-83 31.78914928562598 34.0 31.0 35.5 23.0 37.0 84-85 31.484958997815966 34.0 31.0 35.0 22.5 37.0 86-87 31.171004322939048 34.0 30.0 35.0 21.0 36.0 88-89 30.887033537608392 34.0 30.0 35.0 20.0 36.0 90-91 30.596858952094458 34.0 29.5 35.0 20.0 35.5 92-93 30.379437488103576 34.0 29.0 35.0 19.5 35.0 94-95 30.15567093858601 34.0 29.0 35.0 19.0 35.0 96-97 29.94662553693336 34.0 29.0 35.0 18.0 35.0 98-99 29.77490007005398 33.5 29.0 35.0 18.0 35.0 100 27.75249751278927 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3781697861827027 1101 2 0.22256487469024933 1101 3 0.03950514437280361 1101 4 0.08536074409217775 1101 5 0.1920928552076404 1101 6 0.13676776927458434 1101 7 0.09758255901288493 1101 8 0.10428963219896303 1101 9 0.14070344898956932 1101 10-11 0.10477233658143348 1101 12-13 0.05136692580351365 1101 14-15 0.15754958095173777 1101 16-17 0.20351722600444333 1101 18-19 0.2560530753117476 1101 20-21 0.2094414036996639 1101 22-23 -0.006684357028149179 1101 24-25 0.0658220880451168 1101 26-27 0.13467663674852304 1101 28-29 0.1445530334835894 1101 30-31 0.29502771865787736 1101 32-33 0.41186829655159585 1101 34-35 0.18784336712570848 1101 36-37 0.11975781240364114 1101 38-39 0.2169020150758456 1101 40-41 0.25399385176994826 1101 42-43 0.33202135625786866 1101 44-45 0.36715301625529406 1101 46-47 0.32953050681889096 1101 48-49 0.3059409900647978 1101 50-51 0.3410852141950187 1101 52-53 0.0692920564480275 1101 54-55 -0.12181813216191273 1101 56-57 0.22182392945411777 1101 58-59 0.05258182677115997 1101 60-61 0.2018304011166876 1101 62-63 -0.020013790745203153 1101 64-65 0.2143384930650427 1101 66-67 0.0555533778954711 1101 68-69 0.1452843444576999 1101 70-71 -0.12292892565470481 1101 72-73 -0.0354445845888165 1101 74-75 -0.15574327847956226 1101 76-77 -0.18939442369810067 1101 78-79 0.04403345482278809 1101 80-81 -0.008739604333221962 1101 82-83 -0.1527699222457386 1101 84-85 -0.1572610644777619 1101 86-87 0.23962473695234365 1101 88-89 0.26358447588971856 1101 90-91 0.1342066366406911 1101 92-93 -0.22973800492224683 1101 94-95 -0.2559175616584888 1101 96-97 -0.04950857684409016 1101 98-99 -0.3074068717006533 1101 100 -0.6935529180039346 1102 1 0.21908035528839065 1102 2 -0.04049203587885586 1102 3 0.27168400616141497 1102 4 0.08649895547429054 1102 5 -0.02376080332894759 1102 6 -0.033118409587203246 1102 7 -0.11244996131231488 1102 8 0.042728656589211766 1102 9 0.011093692892004015 1102 10-11 -0.037138232524263515 1102 12-13 0.01996855181977253 1102 14-15 -0.03713334587753536 1102 16-17 0.29214324226460775 1102 18-19 0.0872319371003698 1102 20-21 0.03184790776470692 1102 22-23 0.005364423459653267 1102 24-25 0.033700136825601135 1102 26-27 0.09514005138267123 1102 28-29 0.028317261125863524 1102 30-31 -0.09239504556977352 1102 32-33 -0.08430243515572755 1102 34-35 -0.01157126702064204 1102 36-37 0.32130252785079705 1102 38-39 -0.04826058655017107 1102 40-41 0.2632052338837667 1102 42-43 0.008053876583069552 1102 44-45 -0.06448926016747691 1102 46-47 -0.08816055009167911 1102 48-49 0.007477575430648642 1102 50-51 0.11548358817875481 1102 52-53 0.2346335198626619 1102 54-55 -0.08083439232451184 1102 56-57 0.11752311644599445 1102 58-59 0.1596224771776633 1102 60-61 -0.290535452541846 1102 62-63 -0.12711135172080645 1102 64-65 -0.06510866140650506 1102 66-67 0.0775452478141645 1102 68-69 0.0222680842950993 1102 70-71 0.09255887922334693 1102 72-73 0.047709886955892955 1102 74-75 0.054825827211487876 1102 76-77 0.47674378768401127 1102 78-79 0.025293617424416937 1102 80-81 0.05837421680499233 1102 82-83 -0.1883715482620012 1102 84-85 -0.12096025146963285 1102 86-87 -0.08633461264115283 1102 88-89 -0.3126350363054051 1102 90-91 0.027718831762641116 1102 92-93 0.015115653614337532 1102 94-95 -0.21084439092677698 1102 96-97 -0.09375247928311481 1102 98-99 0.10144678683593256 1102 100 0.23670724459769232 1103 1 0.32281617401540075 1103 2 0.05740898115412563 1103 3 0.07466864247165717 1103 4 0.07999952736214055 1103 5 0.016047227831215594 1103 6 0.13990084912249046 1103 7 0.07874605711174354 1103 8 0.21443411889097774 1103 9 0.2167414718032532 1103 10-11 0.094867393615651 1103 12-13 0.21953422618373963 1103 14-15 0.35203247068557886 1103 16-17 0.40914840471167224 1103 18-19 0.361699463144447 1103 20-21 0.38381782955517707 1103 22-23 0.09499625133686607 1103 24-25 0.21361296256982598 1103 26-27 0.2883724542390169 1103 28-29 0.4639180524949964 1103 30-31 0.272430760482969 1103 32-33 0.2697618326732609 1103 34-35 0.11248975495840341 1103 36-37 0.2931988770424283 1103 38-39 0.2770160835169051 1103 40-41 0.31843491640872656 1103 42-43 0.35250424599171026 1103 44-45 0.42693628621726987 1103 46-47 0.4177206208873301 1103 48-49 0.35797140831574836 1103 50-51 0.27760992902391024 1103 52-53 0.5483681020754076 1103 54-55 0.4605811073818131 1103 56-57 0.30433723743891505 1103 58-59 0.30323201688523227 1103 60-61 0.2767695646147885 1103 62-63 0.5671991370114497 1103 64-65 0.2900723333692241 1103 66-67 0.44996402428329674 1103 68-69 0.6264212265869773 1103 70-71 0.3415387549536604 1103 72-73 0.4803082671222114 1103 74-75 0.40376242494248515 1103 76-77 0.42556755348821085 1103 78-79 0.5789003749748858 1103 80-81 0.6268307378721047 1103 82-83 0.2869677203398062 1103 84-85 0.7254955895146331 1103 86-87 0.5577502122375151 1103 88-89 0.5254666051672849 1103 90-91 0.25735112333270393 1103 92-93 0.5104787251157745 1103 94-95 0.5305425143871396 1103 96-97 0.79839256384032 1103 98-99 0.5873041549153157 1103 100 0.9181657131747727 1104 1 0.265539549388528 1104 2 0.22433536649352703 1104 3 0.1491663465585944 1104 4 -0.09210737976938788 1104 5 -0.04634066027505668 1104 6 0.031944454156189295 1104 7 0.02847144790177225 1104 8 -0.052788692026901174 1104 9 0.12786920490942322 1104 10-11 0.16275958612605734 1104 12-13 0.18295891123155883 1104 14-15 0.21661515472222703 1104 16-17 0.03614564130499076 1104 18-19 0.24522429571247528 1104 20-21 0.14032118512042047 1104 22-23 0.13642311109570926 1104 24-25 0.15778383667899476 1104 26-27 0.1446147059652816 1104 28-29 0.2981759843032563 1104 30-31 0.4741005784028687 1104 32-33 0.23226538216179193 1104 34-35 0.2628634035555777 1104 36-37 0.1209326757916287 1104 38-39 0.20302223365508354 1104 40-41 0.2577351996752242 1104 42-43 0.31225997192271393 1104 44-45 0.2025428158909932 1104 46-47 0.1705359712997634 1104 48-49 0.24579891356206218 1104 50-51 0.21273913040630532 1104 52-53 0.31506801273219054 1104 54-55 0.3962747640129507 1104 56-57 0.7475488839167781 1104 58-59 0.6867949415252568 1104 60-61 0.7297120040310787 1104 62-63 0.600297684664632 1104 64-65 0.6949650868719672 1104 66-67 0.48549873308672176 1104 68-69 0.3654974592118023 1104 70-71 0.3703588703380092 1104 72-73 0.42500896732374827 1104 74-75 0.5222166486606881 1104 76-77 0.11280047611974808 1104 78-79 0.47055440199947185 1104 80-81 0.636642909327982 1104 82-83 0.31348326536810234 1104 84-85 0.1934584255040228 1104 86-87 0.30490888995780807 1104 88-89 0.22510542306640247 1104 90-91 0.2200918825423308 1104 92-93 0.54999059252766 1104 94-95 0.5073738773214735 1104 96-97 0.17763896413951485 1104 98-99 0.4332251865871406 1104 100 0.683106462688233 1105 1 0.254876288818906 1105 2 0.1918654019310182 1105 3 0.21546999498791308 1105 4 0.24031680736106864 1105 5 0.10615260916194558 1105 6 0.18845845468759137 1105 7 0.1677020669214997 1105 8 0.2053159942376297 1105 9 0.16860151577339622 1105 10-11 0.24736460371675406 1105 12-13 0.2752052386330348 1105 14-15 0.34715766531026304 1105 16-17 0.2797087901520712 1105 18-19 0.318364147367987 1105 20-21 0.2419422824342874 1105 22-23 0.21079543207554252 1105 24-25 0.45202068206972257 1105 26-27 0.2609261973812096 1105 28-29 0.4328816802322706 1105 30-31 0.6045022353538343 1105 32-33 0.524237365092894 1105 34-35 0.7317203442785996 1105 36-37 0.4321391832296584 1105 38-39 0.6673062330020301 1105 40-41 0.6107144141600998 1105 42-43 0.3726733773475033 1105 44-45 0.40414598637831745 1105 46-47 0.34617725550079115 1105 48-49 0.5209532923495104 1105 50-51 0.38023811401926366 1105 52-53 0.3940917049541852 1105 54-55 0.233670444288002 1105 56-57 0.44443025634339506 1105 58-59 0.4682637248379393 1105 60-61 0.2812100144734586 1105 62-63 0.5248561565307241 1105 64-65 0.30587452118455616 1105 66-67 0.1877853638357081 1105 68-69 0.541518087867189 1105 70-71 0.6530201077372197 1105 72-73 0.554202164093077 1105 74-75 0.4254518006065524 1105 76-77 0.34980592779574593 1105 78-79 0.5140861789001221 1105 80-81 0.6854871091992933 1105 82-83 0.7453197086857166 1105 84-85 0.7208496560846278 1105 86-87 0.7387829091843301 1105 88-89 0.5366512597210011 1105 90-91 0.3409395013155603 1105 92-93 0.6984711339178205 1105 94-95 0.934077165933779 1105 96-97 1.0851557465302513 1105 98-99 0.7162609666121753 1105 100 0.9638565723299877 1106 1 0.16555469914208487 1106 2 0.22890143175020938 1106 3 0.14614344224514042 1106 4 0.15269149844420582 1106 5 0.1539884064648902 1106 6 0.14666332053183595 1106 7 0.13571408705930565 1106 8 0.07645984496492275 1106 9 0.12113284937641566 1106 10-11 0.3031379071810463 1106 12-13 0.24759903412072504 1106 14-15 0.060433526793133296 1106 16-17 0.08558492426362818 1106 18-19 0.1657532687353438 1106 20-21 0.1621473998311913 1106 22-23 -0.043864240973988444 1106 24-25 0.198841430404876 1106 26-27 -0.13776436905418166 1106 28-29 -0.06417617347966598 1106 30-31 0.1380896142091359 1106 32-33 0.29762071434656434 1106 34-35 0.09836174236748718 1106 36-37 0.0767887601792765 1106 38-39 0.2053739686541789 1106 40-41 0.14602866801752867 1106 42-43 0.1406925361418203 1106 44-45 0.2688106564874033 1106 46-47 0.3260411451167684 1106 48-49 0.14270307903965573 1106 50-51 0.4195262199977208 1106 52-53 0.41614505451379813 1106 54-55 0.2593985022674943 1106 56-57 0.31439452906727183 1106 58-59 0.5077829873127513 1106 60-61 0.40072982084590336 1106 62-63 0.35480825954493156 1106 64-65 0.4530464234325464 1106 66-67 0.3163270722861782 1106 68-69 0.3131779614789778 1106 70-71 0.43973064881338075 1106 72-73 0.37062311367036216 1106 74-75 0.11234182790340697 1106 76-77 0.09274290705625177 1106 78-79 0.4933177875113799 1106 80-81 0.527807784448207 1106 82-83 0.4495685690501965 1106 84-85 0.34947974790134495 1106 86-87 0.7190309265074717 1106 88-89 0.7207895049032587 1106 90-91 0.5695702730043273 1106 92-93 0.845586946721852 1106 94-95 0.8190360166780728 1106 96-97 0.7254082510089113 1106 98-99 0.67466469503049 1106 100 0.7852439872185037 1107 1 0.20055929929131366 1107 2 0.3846472716939928 1107 3 0.36209690467242694 1107 4 0.14457422723824465 1107 5 0.13703667543235554 1107 6 0.26828836852551774 1107 7 0.19388967511775235 1107 8 0.20957802171207618 1107 9 0.25636637033787935 1107 10-11 0.18082102572000736 1107 12-13 0.2562433302978917 1107 14-15 0.3018978955584828 1107 16-17 0.3665471885512588 1107 18-19 0.3827010153866581 1107 20-21 0.2442691190554669 1107 22-23 0.3155178901628659 1107 24-25 0.3851891292436136 1107 26-27 0.5547065992953435 1107 28-29 0.6327065915360208 1107 30-31 0.3497580557365296 1107 32-33 0.28708927033436993 1107 34-35 0.3394838165639058 1107 36-37 0.4616491981714361 1107 38-39 0.6309095057125447 1107 40-41 0.4981211925939135 1107 42-43 0.40162060719419657 1107 44-45 0.4192391585773976 1107 46-47 0.2497402446466097 1107 48-49 0.39060765673146847 1107 50-51 0.17555712430737458 1107 52-53 0.35140067068024194 1107 54-55 0.5255189464897754 1107 56-57 0.5302846036114204 1107 58-59 0.7776342612280871 1107 60-61 0.5326581164724971 1107 62-63 0.6832933253596636 1107 64-65 0.8357317514920126 1107 66-67 0.6934559996183154 1107 68-69 0.6361120598135059 1107 70-71 0.5779886773415512 1107 72-73 0.5785516700928213 1107 74-75 0.918601290808823 1107 76-77 0.8522347897850473 1107 78-79 1.0179285859089333 1107 80-81 0.9880581484757656 1107 82-83 0.8908967444209317 1107 84-85 0.6460535422638038 1107 86-87 0.4909468343306216 1107 88-89 0.3249140639046999 1107 90-91 0.453805887577019 1107 92-93 0.6015091860889861 1107 94-95 0.9276067829108001 1107 96-97 0.808663707800104 1107 98-99 0.6513234653779953 1107 100 0.7485369696365147 1108 1 0.049281303550159805 1108 2 0.0692120410522179 1108 3 0.1035124569870618 1108 4 0.11773734372654587 1108 5 0.015310405481862688 1108 6 -0.01578067679488271 1108 7 0.06462826285201118 1108 8 0.04705380038909368 1108 9 0.05708736123819591 1108 10-11 -0.0560960363619003 1108 12-13 0.06166308668102971 1108 14-15 0.1090687765641718 1108 16-17 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-0.27086956211772417 2316 60-61 -0.041975154438866014 2316 62-63 -0.10365267963408797 2316 64-65 0.1381857152872712 2316 66-67 0.3662478223399077 2316 68-69 0.2941176777910286 2316 70-71 0.02300857434529746 2316 72-73 0.07364569318895775 2316 74-75 -0.05973633403512224 2316 76-77 -0.018873590364766102 2316 78-79 0.014241788156120094 2316 80-81 0.12417706233344461 2316 82-83 0.026313411087595995 2316 84-85 0.07725282441112569 2316 86-87 0.30522195917456685 2316 88-89 0.10559836477860784 2316 90-91 0.20056774775180486 2316 92-93 0.4504772728555295 2316 94-95 0.19256854945262347 2316 96-97 0.0820817009336885 2316 98-99 -0.03437214947843259 2316 100 -0.1215668068928224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 3.0 13 13.0 14 48.0 15 115.0 16 263.0 17 507.0 18 855.0 19 1321.0 20 1934.0 21 2663.0 22 3431.0 23 4151.0 24 4496.0 25 5255.0 26 6174.0 27 7627.0 28 9254.0 29 11345.0 30 13799.0 31 16647.0 32 20635.0 33 26042.0 34 34479.0 35 46695.0 36 68657.0 37 105920.0 38 102647.0 39 14626.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.823711332114065 22.114184569737596 14.14316438904544 22.918939709102897 2 15.739848062094655 22.18474062758327 39.86553750635692 22.209873803965152 3 17.64412689878199 27.112870214657292 30.972541370439345 24.270461516121372 4 12.081121653045006 17.627378067326653 38.968220484492896 31.32327979513545 5 12.310291641646945 38.951879716953364 35.8372678071735 12.900560834226194 6 30.748777482123373 38.43513787175925 18.084826649712326 12.731257996405052 7 26.820079002054527 32.937145682849724 22.915894012444884 17.326881302650865 8 25.610474015117617 36.97282595897992 20.4425396974906 16.974160328411863 9 26.681933332940872 15.050028747642791 20.17417343168363 38.0938644877327 10-11 24.559121048180266 26.63729108901565 29.540214322016773 19.263373540787313 12-13 24.979739289295477 24.27605978724782 29.397555371099692 21.346645552357014 14-15 22.261664380591316 25.940283394851328 26.548889634561533 25.249162589995823 16-17 22.052809632577453 28.817473960172997 27.297470192541663 21.83224621470789 18-19 21.86003112251005 28.178136142339365 28.834828953745983 21.127003781404593 20-21 22.68807466372575 27.414703738190294 28.79551562378886 21.101705974295097 22-23 22.407463005806434 27.31192860380646 28.50745770760606 21.773150682781043 24-25 22.13710199644431 27.364571845700404 28.62230821458146 21.876017943273823 26-27 22.181820679288826 27.551552030831633 28.582543590005226 21.684083699874314 28-29 22.249302152167623 27.688934022105467 28.55392192934689 21.50784189638002 30-31 22.588643227321874 27.358079588068268 28.63359229511832 21.419684889491542 32-33 22.391039855570163 27.45540532585019 28.532742008281165 21.620812810298474 34-35 22.42342060360823 27.642466278965944 28.622085989495382 21.312027127930445 36-37 22.470967539373866 27.607819435559517 28.485563243472356 21.43564978159426 38-39 22.43899375882433 27.734917322328613 28.3973002328299 21.428788686017157 40-41 22.489488944380938 27.575613369768387 28.533260070744188 21.401637615106484 42-43 22.269232844856766 27.531827837767565 28.426981175581734 21.771958141793938 44-45 22.275817137115762 27.745867217181075 28.304067631636787 21.674248014066375 46-47 22.25267204559035 27.626199145769615 28.222276515140365 21.898852293499665 48-49 22.236351629935115 27.698545161610006 28.21583847732083 21.849264731134053 50-51 22.461150801710865 27.653309234417883 28.10938317177444 21.776156792096813 52-53 22.34584084758834 27.791329056667458 27.98844946986481 21.87438062587939 54-55 22.19085744561858 27.831709494794886 28.147941011980105 21.829492047606433 56-57 22.360327672296307 27.822611900246173 27.856462344079457 21.96059808337806 58-59 22.375273746261744 27.71097104372876 28.133364469108862 21.780390740900636 60-61 22.599335717684923 27.91065059437077 27.925074449661235 21.56493923828307 62-63 22.359773054210685 27.868173620201393 28.10799353938483 21.664059786203094 64-65 22.29109062829311 27.582532521434022 28.13032907040407 21.996047779868796 66-67 22.24170514735175 27.674791929555326 27.938645466801947 22.14485745629098 68-69 22.155671583062425 27.735949218286205 28.028656460999677 22.07972273765169 70-71 22.21330423759081 27.581135126436962 28.0436710872833 22.161889548688926 72-73 22.209161306021848 27.794861630604146 27.828516929694768 22.16746013367924 74-75 22.28699093979289 27.845762582149213 27.76560025589149 22.101646222166405 76-77 22.082132211392228 27.713979587631698 27.937687527104966 22.266200673871108 78-79 22.120425655089857 27.650507538255482 27.961653755390603 22.26741305126406 80-81 22.196939591520344 27.83264403004479 27.652591142575396 22.31782523585947 82-83 22.236385420867787 27.900160243672435 27.767392522419886 22.096061813039892 84-85 22.174748859478083 27.91204758152438 27.771819074805876 22.141384484191665 86-87 22.039716434549135 27.890903452715538 27.796106863037572 22.273273249697752 88-89 21.965005937018535 27.76012482458809 27.7868168748712 22.488052363522172 90-91 22.156123468372368 27.712278732186213 27.827193738260707 22.304404061180712 92-93 22.190489089363773 27.664847082285647 27.727560912893534 22.417102915457047 94-95 21.959581148338223 27.736899598677372 27.66034502557217 22.64317422741223 96-97 21.833173210442695 27.717125167687428 27.70044229246258 22.749259329407295 98-99 21.759285609145774 27.56930474461508 27.71110347873019 22.960306167508953 100 20.764903989574666 28.20021667111523 28.02691903094629 23.00796030836382 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 73.5 2 98.5 3 134.0 4 103.0 5 85.0 6 118.0 7 240.5 8 350.5 9 408.5 10 379.0 11 309.5 12 301.5 13 333.0 14 371.5 15 381.5 16 402.5 17 425.0 18 456.5 19 552.5 20 620.5 21 693.5 22 847.0 23 1051.5 24 1328.5 25 1690.5 26 2222.0 27 2881.0 28 3608.0 29 4476.5 30 5687.5 31 6969.0 32 8258.5 33 9670.0 34 11190.0 35 12662.5 36 14342.0 37 15893.0 38 16872.5 39 17905.5 40 18407.0 41 18634.0 42 19060.5 43 19461.0 44 19818.0 45 20353.5 46 20632.0 47 20479.5 48 20525.0 49 20361.5 50 20163.5 51 19358.5 52 17838.5 53 16529.5 54 15235.0 55 13752.0 56 12218.5 57 10569.0 58 8952.0 59 7413.0 60 5872.0 61 4683.0 62 3762.5 63 2836.5 64 2075.5 65 1560.5 66 1136.5 67 839.0 68 651.5 69 507.0 70 361.0 71 274.0 72 218.5 73 169.5 74 130.5 75 90.0 76 70.5 77 51.5 78 34.0 79 23.0 80 18.0 81 11.0 82 7.5 83 6.5 84 4.5 85 1.5 86 0.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002158526079900786 2 0.06279348596075014 3 7.849185745093768E-4 4 3.924592872546884E-4 5 0.001766066792646098 6 5.886889308820326E-4 7 0.0 8 5.886889308820326E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.886889308820326E-4 18-19 0.0015698371490187536 20-21 0.003041559476223835 22-23 0.0 24-25 1.962296436273442E-4 26-27 9.81148218136721E-5 28-29 8.83033396323049E-4 30-31 0.0015698371490187536 32-33 0.003335903941664852 34-35 0.0042189373379879005 36-37 9.81148218136721E-4 38-39 0.0014717223272050816 40-41 0.004807626268869933 42-43 0.005788774487006655 44-45 0.004513281803428916 46-47 0.005003855912497277 48-49 0.006181233774261343 50-51 0.00794730056690744 52-53 0.003924592872546884 54-55 0.0023547557235281302 56-57 0.0024528705453418028 58-59 0.002158526079900786 60-61 0.006769922705143375 62-63 0.011381319330385964 64-65 0.003924592872546884 66-67 0.008634104319603144 68-69 0.010498285934062915 70-71 0.0049057410906836055 72-73 0.005690659665192982 74-75 0.0031396742980375073 76-77 0.002747215010782819 78-79 0.007751070923280097 80-81 0.006769922705143375 82-83 0.013834189875727767 84-85 0.015992715955628553 86-87 0.0186418161445977 88-89 0.018053127213715665 90-91 0.019622964362734422 92-93 0.029434446544101635 94-95 0.03267223566395281 96-97 0.02011353847180278 98-99 0.016090830777442222 100 0.015502141846560194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 509607.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.46175021461855 #Duplication Level Percentage of deduplicated Percentage of total 1 89.68969476669565 74.85658901443333 2 7.608435549273162 12.700266946749217 3 1.3990465506548655 3.5030062124814023 4 0.4830412172896641 1.6126186168318088 5 0.2308227095767329 0.963243366527736 6 0.14955873872966763 0.748946045656135 7 0.09629276064285688 0.5625733635375121 8 0.07209267649016718 0.48135847660205083 9 0.048133474179485734 0.3615573599037027 >10 0.21296013839772393 3.007381103282232 >50 0.00732295264831773 0.41777545904617114 >100 0.001889793033997468 0.23895403275347626 >500 2.3622412924913184E-4 0.14412161546411528 >1k 4.724482584982637E-4 0.40160838673105725 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1028 0.20172407364890985 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 0.1979957104199903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 0.14344386949158863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.81148218136721E-5 0.0 22-23 0.0 0.0 0.0 3.924592872546884E-4 0.0 24-25 0.0 0.0 0.0 3.924592872546884E-4 0.0 26-27 0.0 0.0 0.0 3.924592872546884E-4 0.0 28-29 0.0 0.0 0.0 5.886889308820327E-4 0.0 30-31 0.0 0.0 0.0 0.0023547557235281307 0.0 32-33 0.0 0.0 0.0 0.007554841279652752 0.0 34-35 0.0 0.0 0.0 0.013343615766659406 0.0 36-37 0.0 0.0 0.0 0.025804198136995762 0.0 38-39 0.0 0.0 0.0 0.0408157658744876 0.0 40-41 0.0 0.0 0.0 0.0625972563171228 0.0 42-43 0.0 0.0 0.0 0.08535989497789473 0.0 44-45 0.0 0.0 0.0 0.11106597829307682 0.0 46-47 0.0 0.0 0.0 0.13873435804453235 0.0 48-49 0.0 0.0 0.0 0.1736632346101996 0.0 50-51 0.0 0.0 0.0 0.20702227402684814 0.0 52-53 0.0 0.0 0.0 0.24460025078148456 0.0 54-55 0.0 0.0 0.0 0.2915972504302335 0.0 56-57 0.0 0.0 0.0 0.3328054755919758 0.0 58-59 0.0 0.0 0.0 0.384806331153222 0.0 60-61 0.0 0.0 0.0 0.4457356354995124 0.0 62-63 0.0 0.0 0.0 0.5163783072053563 0.0 64-65 0.0 0.0 0.0 0.5883945864165916 0.0 66-67 0.0 0.0 0.0 0.6695355440564985 0.0 68-69 0.0 0.0 0.0 0.7602917542341452 0.0 70-71 0.0 0.0 0.0 0.8744974068252594 2.9434446544101634E-4 72-73 0.0 0.0 0.0 0.987721911198237 3.924592872546884E-4 74-75 0.0 0.0 0.0 1.0963350189459722 3.924592872546884E-4 76-77 0.0 0.0 0.0 1.2200578092530128 3.924592872546884E-4 78-79 0.0 0.0 0.0 1.372233897886018 3.924592872546884E-4 80-81 0.0 0.0 0.0 1.527844005282502 3.924592872546884E-4 82-83 0.0 0.0 0.0 1.6962090395147635 3.924592872546884E-4 84-85 0.0 0.0 0.0 1.8696760444813356 3.924592872546884E-4 86-87 0.0 0.0 0.0 2.0599206839780457 3.924592872546884E-4 88 0.0 0.0 0.0 2.212685461541933 3.924592872546884E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 810 0.0 40.650555 1 GTACCTG 495 0.0 32.30927 1 GTATCAA 1330 0.0 31.476892 1 TACCTGG 535 0.0 29.893627 2 TACCCGG 70 0.00879629 26.87914 2 CAGTGCG 105 7.421827E-5 26.85277 9 CGGCTAT 70 0.0088385055 26.85277 6 ACCGTGA 70 0.0088385055 26.85277 8 ACCTGGG 575 0.0 26.152264 3 TAGACTG 205 1.0804797E-9 25.215406 5 ATCAACG 1680 0.0 24.335323 3 CATGGGG 875 0.0 24.167492 4 CAACGCA 1675 0.0 24.127415 5 TCAACGC 1675 0.0 24.127415 4 TATCAAC 1740 0.0 23.789587 2 AACGCAG 1720 0.0 23.769384 6 GTACATG 1745 0.0 23.721422 1 ACATGGG 1730 0.0 22.817093 3 TACATGG 1795 0.0 22.53655 2 ATGGGGG 565 0.0 21.624796 5 >>END_MODULE