FastQCFastQC Report
Mon 6 Jun 2016
ERR1211549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11409026
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT456270.39992020353008223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362130.3174065866797043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249870.2190108077586991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203050.054.89811
GTATCAA407000.041.6784321
ATCAACG490050.033.6972963
CAACGCA495500.033.2847825
TCAACGC496500.033.2780274
AACGCAG511150.032.294256
TATCAAC522600.031.7110542
GTACATG568500.030.5342271
TACATGG576750.029.3779072
ACATGGG563700.028.761053
ACGCAGA583450.028.0923587
CGCAGAG585700.027.888568
CATGGGA317050.024.7523654
GCAGAGT651250.024.4925679
CATGGGG203600.024.188644
GAGTACT372950.021.73238612-13
AGAGTAC621550.020.9870110-11
CAGAGTA657850.020.10390710-11
AGTACTT408900.019.75850312-13
GTACTTT411200.019.51343214-15
Produced by FastQC (version 0.11.5)