FastQCFastQC Report
Mon 6 Jun 2016
ERR1211549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11409026
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492940.4320614222458604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400480.35102032373315656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297490.26074969063967424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216650.054.0506441
GTATCAA411850.039.7717481
ATCAACG510750.031.3006233
TCAACGC518250.030.9108474
CAACGCA521150.030.7025015
AACGCAG539900.029.7493936
TATCAAC541500.029.7105732
GTACATG570650.029.4375651
TACATGG580550.028.4654852
ACATGGG562600.028.0567473
ACGCAGA618450.025.8035057
CGCAGAG621100.025.6253198
CATGGGG206800.024.5412374
CATGGGA311150.023.4393254
GCAGAGT691600.022.6459339
AGAGTAC655250.020.93344910-11
GAGTACT400000.020.5940312-13
ACGGGCG9150.020.5427385
TACCTGG75700.020.1903532
AGTACTT433300.020.08499312-13
Produced by FastQC (version 0.11.5)