##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11409026 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13543014101291 33.0 31.0 34.0 30.0 34.0 2 32.345293805097825 34.0 31.0 34.0 30.0 34.0 3 32.356821607734084 34.0 31.0 34.0 30.0 34.0 4 35.99089194818208 37.0 35.0 37.0 35.0 37.0 5 35.92788832280687 37.0 35.0 37.0 35.0 37.0 6 35.94819925907785 37.0 35.0 37.0 35.0 37.0 7 35.878438965780255 37.0 35.0 37.0 35.0 37.0 8 35.8744942819834 37.0 35.0 37.0 35.0 37.0 9 37.52828024057444 39.0 37.0 39.0 35.0 39.0 10-11 37.49323176229066 39.0 37.0 39.0 35.0 39.0 12-13 37.419381330185416 39.0 37.0 39.0 34.0 39.0 14-15 38.644714062357295 40.0 38.0 41.0 34.0 41.0 16-17 38.593082178969524 40.0 38.0 41.0 34.0 41.0 18-19 38.61505666653753 40.0 38.0 41.0 34.0 41.0 20-21 38.57404966033034 40.0 38.0 41.0 34.0 41.0 22-23 38.47236766749414 40.0 38.0 41.0 34.0 41.0 24-25 38.37038819089378 40.0 38.0 41.0 34.0 41.0 26-27 38.20308872115814 40.0 38.0 41.0 34.0 41.0 28-29 38.23412143157532 40.0 38.0 41.0 34.0 41.0 30-31 38.15099321361876 40.0 37.5 41.0 33.5 41.0 32-33 37.96658741946946 40.0 37.0 41.0 33.0 41.0 34-35 37.85583878939359 40.0 37.0 41.0 33.0 41.0 36-37 37.79694024713415 40.0 37.0 41.0 33.0 41.0 38-39 37.706532003696026 40.0 37.0 41.0 33.0 41.0 40-41 37.55323342237979 40.0 37.0 41.0 32.0 41.0 42-43 37.406221705516316 40.0 37.0 41.0 31.5 41.0 44-45 37.21967361631046 40.0 36.0 41.0 31.0 41.0 46-47 36.95508542972905 40.0 36.0 41.0 30.5 41.0 48-49 36.72305195027165 39.0 36.0 41.0 30.0 41.0 50-51 36.533506497399515 39.0 36.0 41.0 29.5 41.0 52-53 36.35499274872369 39.0 35.0 41.0 29.0 41.0 54-55 36.18372633211634 39.0 35.0 40.0 28.0 41.0 56-57 36.01384215444859 39.0 35.0 40.0 28.0 41.0 58-59 35.78719283311301 38.5 35.0 40.0 28.0 41.0 60-61 35.508970967372676 38.0 34.0 40.0 27.0 41.0 62-63 35.21603491831818 38.0 34.0 40.0 26.0 41.0 64-65 34.928658721612166 37.5 34.0 40.0 26.0 41.0 66-67 34.61633543476893 37.0 33.5 40.0 26.0 41.0 68-69 34.241640522162015 36.5 33.0 39.0 25.5 41.0 70-71 33.87258539861334 36.0 32.5 39.0 25.0 40.0 72-73 33.47357429985698 35.5 32.0 38.5 24.0 40.0 74-75 33.05962231131737 35.0 32.0 38.0 23.5 40.0 76-77 31.454853332791075 34.0 29.5 36.0 21.5 38.5 78-79 32.28664449533203 35.0 31.0 37.0 22.5 39.0 80-81 32.23904051932216 35.0 31.0 36.0 23.0 39.0 82-83 31.934703014963766 35.0 31.0 36.0 22.0 37.5 84-85 31.607277343394607 34.0 31.0 35.5 21.5 37.0 86-87 31.303246482215044 34.0 31.0 35.0 20.0 37.0 88-89 31.00320697840464 34.0 30.0 35.0 20.0 36.0 90-91 30.710308969407205 34.0 30.0 35.0 19.5 36.0 92-93 30.490586838876517 34.0 30.0 35.0 19.0 35.5 94-95 30.264080956604005 34.0 29.0 35.0 18.5 35.0 96-97 30.063220953304867 34.0 29.0 35.0 18.0 35.0 98-99 29.90543079663417 34.0 29.0 35.0 18.0 35.0 100 28.05787759621198 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30866796822963494 1101 2 0.23376231639174705 1101 3 0.29126855869768065 1101 4 0.19004539314546776 1101 5 0.21901414673695996 1101 6 0.22405748158833916 1101 7 0.2641286663595537 1101 8 0.20086294027432672 1101 9 0.22882669541193934 1101 10-11 0.2505257034539241 1101 12-13 0.24036060274849547 1101 14-15 0.3360541102698136 1101 16-17 0.32786522520391514 1101 18-19 0.3695964009643049 1101 20-21 0.3327319444246797 1101 22-23 0.2987579460987817 1101 24-25 0.30736083438799966 1101 26-27 0.25110407402087276 1101 28-29 0.30392431008883847 1101 30-31 0.3550623983895065 1101 32-33 0.3865781458852311 1101 34-35 0.3886022781653793 1101 36-37 0.385090562657723 1101 38-39 0.3282841884617014 1101 40-41 0.36168236132549936 1101 42-43 0.40532299354254775 1101 44-45 0.3980241841366663 1101 46-47 0.41115240382844576 1101 48-49 0.36900336158265645 1101 50-51 0.3527875466257697 1101 52-53 0.30602351110460546 1101 54-55 0.332266554222862 1101 56-57 0.35072397112701736 1101 58-59 0.3841765359653735 1101 60-61 0.2927989772571209 1101 62-63 0.23980166596418684 1101 64-65 0.27399115413967223 1101 66-67 0.22151029347037365 1101 68-69 0.25525335481628275 1101 70-71 0.23636173802432126 1101 72-73 0.20446557846545943 1101 74-75 0.24744336753949625 1101 76-77 0.35378586545666124 1101 78-79 0.4332372051697533 1101 80-81 0.3766018447283983 1101 82-83 0.3344445972362422 1101 84-85 0.33317710310025106 1101 86-87 0.27756005644015147 1101 88-89 0.27572760649491457 1101 90-91 0.18130739869864954 1101 92-93 0.11498396068503425 1101 94-95 0.20088750732614713 1101 96-97 0.11966691903271354 1101 98-99 -0.012878518631840308 1101 100 -0.04221913544576239 1102 1 0.3078505872388533 1102 2 0.24415005238227394 1102 3 0.31699741302189466 1102 4 0.21167941292213754 1102 5 0.1848052153996207 1102 6 0.19672617220625455 1102 7 0.1889566378447043 1102 8 0.18656684492013653 1102 9 0.21889737599965997 1102 10-11 0.21275752371195011 1102 12-13 0.25814288413042874 1102 14-15 0.3364048191716833 1102 16-17 0.3344496547070577 1102 18-19 0.3015793719690052 1102 20-21 0.322415274173558 1102 22-23 0.31613977351052114 1102 24-25 0.28585577018120745 1102 26-27 0.2828750192102021 1102 28-29 0.35059820819048326 1102 30-31 0.3726787215408649 1102 32-33 0.4676690464683517 1102 34-35 0.38548885581504777 1102 36-37 0.49752572288335983 1102 38-39 0.4630287951735994 1102 40-41 0.48051476075141863 1102 42-43 0.4513569392657999 1102 44-45 0.43040225584478975 1102 46-47 0.484887277353387 1102 48-49 0.5112362322428652 1102 50-51 0.47794046500986553 1102 52-53 0.46197256625650596 1102 54-55 0.46582122510663027 1102 56-57 0.5185959043804189 1102 58-59 0.5131223125055939 1102 60-61 0.5251919917876293 1102 62-63 0.5651503162093974 1102 64-65 0.5146957370726639 1102 66-67 0.47204931598145805 1102 68-69 0.5112016258687007 1102 70-71 0.49793841627060687 1102 72-73 0.47621974139452306 1102 74-75 0.435851584461588 1102 76-77 0.4840196250667219 1102 78-79 0.5231192152216195 1102 80-81 0.5033489689881847 1102 82-83 0.4482141378306608 1102 84-85 0.33767929283252585 1102 86-87 0.3545992700968412 1102 88-89 0.33346623676134257 1102 90-91 0.3497927679719126 1102 92-93 0.29746551200769034 1102 94-95 0.33760725050300167 1102 96-97 0.3693883991766036 1102 98-99 0.39834847845877874 1102 100 0.4279430821429955 1103 1 0.2957182874541715 1103 2 0.24208553175695613 1103 3 0.3175215594323788 1103 4 0.16589026985241162 1103 5 0.16542730066809241 1103 6 0.18982495998275084 1103 7 0.15939564209511303 1103 8 0.15926923899953493 1103 9 0.18135391221882458 1103 10-11 0.20489344119378217 1103 12-13 0.25675943331328455 1103 14-15 0.32163764430505637 1103 16-17 0.3175164574112088 1103 18-19 0.3226405909452481 1103 20-21 0.3071560678003067 1103 22-23 0.2973177365852635 1103 24-25 0.2891712816760261 1103 26-27 0.2683488058969701 1103 28-29 0.34040915317670795 1103 30-31 0.39075784408336744 1103 32-33 0.427714560957277 1103 34-35 0.40800178692785494 1103 36-37 0.4363327410087976 1103 38-39 0.3576300441178475 1103 40-41 0.4281555849478167 1103 42-43 0.47405682588833997 1103 44-45 0.44758005957972813 1103 46-47 0.5087528632730951 1103 48-49 0.47988425690003567 1103 50-51 0.5249673570076681 1103 52-53 0.49043559085860977 1103 54-55 0.5485211340537077 1103 56-57 0.5460256553355123 1103 58-59 0.5803952599170614 1103 60-61 0.5785286454902305 1103 62-63 0.631040402096616 1103 64-65 0.6175964287728206 1103 66-67 0.5849623995446507 1103 68-69 0.5475918153904118 1103 70-71 0.5915987583964792 1103 72-73 0.5433959449965755 1103 74-75 0.5309937550207664 1103 76-77 0.6015051413386168 1103 78-79 0.5651078743328313 1103 80-81 0.51847327405752 1103 82-83 0.5219464618655181 1103 84-85 0.4352893815069514 1103 86-87 0.3961929501880732 1103 88-89 0.43753614169037647 1103 90-91 0.4971493228875694 1103 92-93 0.4424025581282258 1103 94-95 0.5150941516443837 1103 96-97 0.4609350786383466 1103 98-99 0.45440575091866364 1103 100 0.6529469393612075 1104 1 0.25554983041907775 1104 2 0.19786973815860165 1104 3 0.24995494587834344 1104 4 0.06619688716713057 1104 5 0.10970112320266878 1104 6 0.1250073576772408 1104 7 0.1695256747263656 1104 8 0.1958681258002457 1104 9 0.1945342299248196 1104 10-11 0.21882767416673232 1104 12-13 0.22899431478190024 1104 14-15 0.2838858979848169 1104 16-17 0.2618793318423016 1104 18-19 0.2593251047136462 1104 20-21 0.1907598618420252 1104 22-23 0.2662682836857968 1104 24-25 0.23403641030234468 1104 26-27 0.2718506269923324 1104 28-29 0.36187446363151565 1104 30-31 0.2675812908013313 1104 32-33 0.2811262209888028 1104 34-35 0.3596806690490766 1104 36-37 0.26383959217795194 1104 38-39 0.3359930188236575 1104 40-41 0.47853023618775836 1104 42-43 0.5257303083132499 1104 44-45 0.4325185603737154 1104 46-47 0.3717463237574776 1104 48-49 0.43629253854278716 1104 50-51 0.4170796447604417 1104 52-53 0.4027189856570459 1104 54-55 0.48024425996000986 1104 56-57 0.5095380037777275 1104 58-59 0.44856708912058707 1104 60-61 0.47849143967156493 1104 62-63 0.4412847126865529 1104 64-65 0.560990524240502 1104 66-67 0.5641881452966686 1104 68-69 0.5485668708918681 1104 70-71 0.4203070326272993 1104 72-73 0.5394055844100265 1104 74-75 0.592409623592836 1104 76-77 0.49999100054197143 1104 78-79 0.5675116947247716 1104 80-81 0.6210853593497916 1104 82-83 0.514711918018449 1104 84-85 0.5482303813748253 1104 86-87 0.5224380186090869 1104 88-89 0.5718877618203528 1104 90-91 0.465756103062521 1104 92-93 0.4677231056102187 1104 94-95 0.5504063668815995 1104 96-97 0.4173732440965843 1104 98-99 0.4598467787030813 1104 100 0.6174696980132168 1105 1 0.22814038642877676 1105 2 0.19123375669512654 1105 3 0.2518855356534857 1105 4 0.11114969019568832 1105 5 0.11239226102592426 1105 6 0.1443609275999833 1105 7 0.1761920172374829 1105 8 0.17829832449618266 1105 9 0.21249819219828936 1105 10-11 0.20775882756162645 1105 12-13 0.21200793639147975 1105 14-15 0.32985416011312196 1105 16-17 0.3109751433189203 1105 18-19 0.2968753478721524 1105 20-21 0.23577528928714742 1105 22-23 0.31693449942622465 1105 24-25 0.30476865333164227 1105 26-27 0.29188842383339875 1105 28-29 0.32306644368849646 1105 30-31 0.38369373138137775 1105 32-33 0.4082676925895399 1105 34-35 0.45611791424740744 1105 36-37 0.44083633898028296 1105 38-39 0.43954230138601247 1105 40-41 0.4535164995257972 1105 42-43 0.4027935564629175 1105 44-45 0.45485563587738653 1105 46-47 0.49661130650456187 1105 48-49 0.5594649499534086 1105 50-51 0.6355502865024434 1105 52-53 0.5683524826128377 1105 54-55 0.4924672829326724 1105 56-57 0.5134477305671084 1105 58-59 0.5974779645315564 1105 60-61 0.5928482659878824 1105 62-63 0.5866822289702327 1105 64-65 0.6529716389079354 1105 66-67 0.5791957861603265 1105 68-69 0.6198045416622975 1105 70-71 0.6327106581777286 1105 72-73 0.6241252724232496 1105 74-75 0.712486672931206 1105 76-77 0.6357128922802566 1105 78-79 0.7065739294698474 1105 80-81 0.6477317442278832 1105 82-83 0.6253341832889276 1105 84-85 0.6314167033195766 1105 86-87 0.6282156803199008 1105 88-89 0.6215673576516529 1105 90-91 0.6553759690493131 1105 92-93 0.6050857684764992 1105 94-95 0.7277722591284927 1105 96-97 0.6795991585975933 1105 98-99 0.75714803539935 1105 100 0.8440601318178018 1106 1 0.260919760815554 1106 2 0.22599913945926886 1106 3 0.26540961997532264 1106 4 0.17899320016667275 1106 5 0.16421037124634097 1106 6 0.14678760315307215 1106 7 0.20290771849409595 1106 8 0.18585707628547965 1106 9 0.19348625016615273 1106 10-11 0.21071666219871332 1106 12-13 0.227633903582948 1106 14-15 0.2744826621219403 1106 16-17 0.3452382486292649 1106 18-19 0.3161353258857744 1106 20-21 0.3213301206754551 1106 22-23 0.3041765709064421 1106 24-25 0.36524963841252145 1106 26-27 0.318221530482667 1106 28-29 0.38097994077023145 1106 30-31 0.3843922550661105 1106 32-33 0.4113236745067823 1106 34-35 0.3864795385487412 1106 36-37 0.38162842677456155 1106 38-39 0.40937165928087893 1106 40-41 0.40755981369711236 1106 42-43 0.37067389727852174 1106 44-45 0.41367635638628997 1106 46-47 0.4714379306970642 1106 48-49 0.5075372287267896 1106 50-51 0.4771914961579071 1106 52-53 0.5477225410074738 1106 54-55 0.44247024262315904 1106 56-57 0.4846589024147079 1106 58-59 0.5832898250301071 1106 60-61 0.5826914047120013 1106 62-63 0.5045772831785769 1106 64-65 0.5895811388919086 1106 66-67 0.6284819014693284 1106 68-69 0.5586080016274693 1106 70-71 0.5666349978178147 1106 72-73 0.5900074548584158 1106 74-75 0.5926282362223176 1106 76-77 0.5868432504654457 1106 78-79 0.6682089947228107 1106 80-81 0.6892173159204305 1106 82-83 0.6890953212267164 1106 84-85 0.6313305900520056 1106 86-87 0.5820641111484548 1106 88-89 0.6506667406557476 1106 90-91 0.7074874481982256 1106 92-93 0.63257323174183 1106 94-95 0.7286971092426917 1106 96-97 0.7606222031614038 1106 98-99 0.785929797798179 1106 100 0.8624651343172047 1107 1 0.20886659562591348 1107 2 0.19067027673561654 1107 3 0.22111415760834063 1107 4 0.16583998100585262 1107 5 0.1560670384287235 1107 6 0.16345667431988886 1107 7 0.1660309353089744 1107 8 0.16789940668468972 1107 9 0.24263092960276111 1107 10-11 0.2054941788740834 1107 12-13 0.22082507329304946 1107 14-15 0.26511473547939346 1107 16-17 0.22611366353412876 1107 18-19 0.17718961819350199 1107 20-21 0.2388220867666746 1107 22-23 0.25911831523865914 1107 24-25 0.2921997963390268 1107 26-27 0.28342645492271146 1107 28-29 0.306015766437703 1107 30-31 0.19101445025031438 1107 32-33 0.23945715510512144 1107 34-35 0.2622145653623349 1107 36-37 0.34904068432879143 1107 38-39 0.35700392098259925 1107 40-41 0.35352540368776175 1107 42-43 0.32826814849155994 1107 44-45 0.30896696233556753 1107 46-47 0.2920582137942631 1107 48-49 0.41368837135296843 1107 50-51 0.34260277042884724 1107 52-53 0.313202067079402 1107 54-55 0.328926353017863 1107 56-57 0.3839147378081833 1107 58-59 0.5008377840268778 1107 60-61 0.41315395675369615 1107 62-63 0.42358550866597966 1107 64-65 0.586080805852319 1107 66-67 0.543769046758328 1107 68-69 0.5000546248794038 1107 70-71 0.5708952953345019 1107 72-73 0.4456167066311849 1107 74-75 0.4773571618170749 1107 76-77 0.4448887623615647 1107 78-79 0.5385154099390945 1107 80-81 0.5536915868489842 1107 82-83 0.5998029965791147 1107 84-85 0.46045333799429145 1107 86-87 0.36740001131289546 1107 88-89 0.39102221736822784 1107 90-91 0.3678238602215025 1107 92-93 0.39964601858243043 1107 94-95 0.6277541258065611 1107 96-97 0.6702608502105925 1107 98-99 0.6637347619564906 1107 100 0.8550976706917695 1108 1 0.22793542310478898 1108 2 0.16646446414984695 1108 3 0.2299755168250357 1108 4 0.13992903538659363 1108 5 0.10320620275341952 1108 6 0.12901415354041745 1108 7 0.11998409525099873 1108 8 0.15350166311089453 1108 9 0.11187185477977124 1108 10-11 0.12983761290826834 1108 12-13 0.18874793185592154 1108 14-15 0.20933944337784283 1108 16-17 0.23477985089068198 1108 18-19 0.2552931989102234 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-0.18436168295794175 2316 68-69 -0.2452791890280892 2316 70-71 -0.4155041663751433 2316 72-73 -0.49157677642362785 2316 74-75 -0.5401655201807145 2316 76-77 -0.49975309274929103 2316 78-79 -0.4005730045465228 2316 80-81 -0.27173272789092096 2316 82-83 -0.27893779552075415 2316 84-85 -0.2999720652734901 2316 86-87 -0.31588524747183655 2316 88-89 -0.30240610702962556 2316 90-91 -0.23342549553211356 2316 92-93 -0.1147494443804078 2316 94-95 -0.07052902018663332 2316 96-97 -0.0814676845674498 2316 98-99 -0.20195651479275867 2316 100 -0.40830234674053045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 18.0 11 58.0 12 98.0 13 336.0 14 1023.0 15 2871.0 16 6842.0 17 15038.0 18 27501.0 19 42309.0 20 58882.0 21 81673.0 22 103838.0 23 109770.0 24 109839.0 25 116618.0 26 132556.0 27 159599.0 28 192382.0 29 233281.0 30 282286.0 31 341593.0 32 421235.0 33 527042.0 34 690520.0 35 937909.0 36 1387221.0 37 2275388.0 38 2661625.0 39 489588.0 40 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.45802934311467 21.49660175201796 14.00002436742643 23.045344537440947 2 14.003090639701721 22.74244540507238 41.38876696843295 21.865696986792944 3 16.356833237414286 29.490400761649255 31.485855899144234 22.66691010179223 4 10.648498709478197 18.875854581196815 41.174495358951056 29.301151350373928 5 11.074078485850338 40.26109885309302 36.54310216297703 12.121720498079606 6 27.45030410797987 40.96083939780213 19.407515561698496 12.181340932519504 7 24.953260690264006 34.11718932010498 24.536126046167304 16.393423943463713 8 24.686553781062933 36.85959946164812 21.663903471808617 16.789943285480323 9 26.492860342706003 15.015837029312157 21.56079824903469 36.93050437894715 10-11 22.961811113411436 27.794042190805772 30.47083510897425 18.77331158680855 12-13 24.199550426127526 25.837205559878644 30.148480685380154 19.814763328613676 14-15 21.54593652429226 26.83452995899913 28.08987375434152 23.529659762367096 16-17 20.291170847416772 29.78142793752705 28.794438570638153 21.132962644418026 18-19 20.189353223203714 28.75859653123996 31.0397826186326 20.012267626923723 20-21 21.336415080852703 27.926249302230755 30.913242931685737 19.82409268523081 22-23 20.84708286435156 28.14037009558792 30.51254903409586 20.499998005964663 24-25 20.545429652878283 28.313543735204693 30.868819278493937 20.272207333423086 26-27 20.581761180624632 28.394629105247198 30.74156775107116 20.28204196305701 28-29 20.444539340835874 28.153607514952757 31.06492683716112 20.336926307050245 30-31 20.800217059477795 28.22781200338737 30.80799216082561 20.163978776309225 32-33 20.597370101097063 28.314119689207075 30.738911774222622 20.349598435473244 34-35 20.795704568517507 28.461835406306513 30.79005702155403 19.952403003621953 36-37 20.846088384318914 28.17876760722942 30.61004065342441 20.365103355027255 38-39 20.61576320137437 28.52504853688146 30.66150400785355 20.197684253890618 40-41 20.74697522176068 28.27687194571488 30.838901692855508 20.13725113966893 42-43 20.630872585585156 28.271132016543536 30.540473566423586 20.557521831447715 44-45 20.69093161351489 28.561952417951225 30.302473559684778 20.44464240884911 46-47 20.76020627551002 28.584513839043268 29.945371026943434 20.709908858503276 48-49 20.65668703013224 28.695840143836783 29.987954432225898 20.659518393805083 50-51 20.844744372488577 28.761157032363837 29.611157367654993 20.782941227492596 52-53 20.8565553764594 28.856492441044722 29.513409319227268 20.773542863268606 54-55 20.809816291849025 28.943123294510492 29.517651717787086 20.729408695853394 56-57 20.819447425830827 28.948752400460382 29.37247107461125 20.859329099097543 58-59 20.70844791736241 28.784875486669858 29.689907436965857 20.816769159001876 60-61 20.887352884676368 29.070455600426698 29.43240458237588 20.609786932521057 62-63 20.73772250094698 28.85916759874435 29.63060707295523 20.77250282735344 64-65 20.59858279936963 28.764105542975805 29.58227283456868 21.055038823085884 66-67 20.523089076777048 28.881994247410443 29.367708673380477 21.227208002432036 68-69 20.60372357141874 28.947062758508906 29.40574827641736 21.04346539365499 70-71 20.67123071894342 28.64157998184302 29.442555027781133 21.24463427143243 72-73 20.62436494983742 28.8459588928192 29.240772014126332 21.288904143217042 74-75 20.637531153949112 28.899728504629724 29.117768896030437 21.344971445390723 76-77 20.437844335269155 28.842858190306465 29.354350922607825 21.36494655181655 78-79 20.517012654872467 28.737052177681534 29.23344526553774 21.512489901908257 80-81 20.406738959335836 29.019085057794076 29.015872498290506 21.558303484579582 82-83 20.61198152609829 28.890399071237855 29.078581584606166 21.419037818057685 84-85 20.482802632254323 28.893829963005206 28.982307295077664 21.6410601096628 86-87 20.212954776677023 28.919349324623738 29.253305955412333 21.614389943286902 88-89 20.47905566517259 28.708727696031115 29.116731884564935 21.695484754231362 90-91 20.51631494359381 28.856310304832217 29.062937281199126 21.564437470374852 92-93 20.47727093177689 28.72346479346724 28.999335455305182 21.799928819450688 94-95 20.187856680221834 28.533041083186816 29.067966879934037 22.211135356657312 96-97 20.296409925236254 28.489898955804467 29.139506357815844 22.07418476114344 98-99 20.140123019130098 28.470010662534616 29.061756129313643 22.328110189021643 100 19.49143329846501 28.800679318619437 29.188853872505376 22.519033510410175 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2477.0 1 1828.0 2 1827.0 3 2552.0 4 2246.5 5 2289.5 6 3551.0 7 6666.0 8 10853.5 9 13355.5 10 12715.5 11 11589.0 12 12312.0 13 13518.5 14 14752.0 15 15928.5 16 17078.5 17 18400.0 18 20071.5 19 22372.0 20 25458.0 21 29735.0 22 35552.0 23 43208.5 24 53351.0 25 67423.0 26 86442.5 27 107885.0 28 130788.0 29 158767.5 30 191013.0 31 221767.5 32 257749.0 33 298837.5 34 341224.5 35 378888.0 36 419492.5 37 454853.0 38 468606.5 39 476209.0 40 464231.0 41 444319.5 42 432238.0 43 424845.0 44 425711.5 45 433282.5 46 429171.5 47 410421.5 48 397704.0 49 384200.0 50 367690.5 51 338112.5 52 301479.0 53 274184.0 54 247724.5 55 218564.0 56 190111.0 57 160798.5 58 133376.5 59 110969.0 60 89024.0 61 69759.5 62 54916.5 63 41959.0 64 30085.0 65 21037.5 66 15114.0 67 11380.0 68 8777.5 69 6730.5 70 5173.5 71 3814.5 72 2661.0 73 1912.0 74 1500.0 75 1093.5 76 747.5 77 491.5 78 352.0 79 271.0 80 175.5 81 124.5 82 93.5 83 70.5 84 55.5 85 45.0 86 33.5 87 17.5 88 10.5 89 6.5 90 5.0 91 5.0 92 4.5 93 6.0 94 5.5 95 5.0 96 8.5 97 7.0 98 4.0 99 4.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030940415071365425 2 0.07143466935740175 3 8.76499010520267E-4 4 6.573742578902003E-4 5 0.0014637533475688458 6 7.45024158942227E-4 7 0.0 8 0.0011482137037815497 9 4.3824950526013353E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.757016243104364E-4 18-19 0.0011964211493601645 20-21 0.003045834061557928 22-23 4.382495052601335E-6 24-25 1.752998021040534E-5 26-27 1.270923565254387E-4 28-29 8.063790896786457E-4 30-31 0.0015031958030422578 32-33 0.0029494191704006983 34-35 0.004671739726073023 36-37 0.0012884535454647924 38-39 0.0015426382585156698 40-41 0.0058637783803805864 42-43 0.007248646817002609 44-45 0.006047843172589842 46-47 0.0077613987381569645 48-49 0.009602046660249525 50-51 0.00903232230341135 52-53 0.004426320003127348 54-55 0.0024366672492463423 56-57 0.0024279022591411395 58-59 0.0032255163587145827 60-61 0.008120763332470273 62-63 0.01503195803042258 64-65 0.004198430260392079 66-67 0.011341897196132255 68-69 0.013471789791696503 70-71 0.006512387648165584 72-73 0.008506422897099191 74-75 0.003155396437872961 76-77 0.00315101394282036 78-79 0.008151440797838484 80-81 0.005933898301222208 82-83 0.01766145506198338 84-85 0.01884034623113314 86-87 0.024143165244780756 88-89 0.02320969379857667 90-91 0.02598381316687332 92-93 0.037032083194481286 94-95 0.04067393658319299 96-97 0.024796157007618353 98-99 0.019839555103126245 100 0.02017700722217655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1409026E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.285695994326936 #Duplication Level Percentage of deduplicated Percentage of total 1 67.6100650924713 31.969889841192533 2 16.785499416060016 15.874280450015323 3 6.619418498743144 9.39011432372377 4 3.2620252398950798 6.169885352780006 5 1.7779052166703435 4.203474279110091 6 1.123127587300856 3.18647217935701 7 0.7101960430974491 2.350747993319592 8 0.4911408080909728 1.8579147953438249 9 0.34453128549107637 1.466226146363913 >10 1.1747073234773926 8.719893078034136 >50 0.04434237037421638 1.4540367967854337 >100 0.04489298790834509 4.6883947916081885 >500 0.007233527420768617 2.4046188429461925 >1k 0.004706943717371341 4.031613803190564 >5k 1.0382964080345538E-4 0.28345085433208134 >10k+ 1.0382964080345538E-4 1.948986471897338 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49294 0.4320614222458604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40048 0.35102032373315656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29749 0.26074969063967424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.505996042081068E-5 0.0 10-11 0.0 0.0 0.0 3.505996042081068E-5 0.0 12-13 8.76499010520267E-6 0.0 0.0 3.505996042081068E-5 0.0 14-15 1.752998021040534E-5 0.0 0.0 4.3824950526013353E-5 0.0 16-17 3.0677465368209345E-5 0.0 0.0 5.258994063121602E-5 0.0 18-19 3.505996042081068E-5 0.0 0.0 8.764990105202671E-5 0.0 20-21 4.3824950526013353E-5 0.0 0.0 1.1832736642023604E-4 0.0 22-23 4.3824950526013353E-5 0.0 0.0 2.235072476826681E-4 0.0 24-25 6.573742578902002E-5 0.0 0.0 4.6454447557574155E-4 0.0 26-27 8.764990105202671E-5 0.0 0.0 7.231116836792202E-4 0.0 28-29 1.0079738620983071E-4 0.0 0.0 0.001972122773670601 0.0 30-31 1.1832736642023605E-4 0.0 0.0 0.006911194697952305 0.0 32-33 1.2270986147283738E-4 0.0 0.0 0.01644312143736021 0.0 34-35 1.5338732684104674E-4 0.0 0.0 0.03171173420062326 0.0 36-37 1.8406479220925607E-4 0.0 0.0 0.05664813104992486 0.0 38-39 1.8406479220925607E-4 0.0 0.0 0.09796191191079764 0.0 40-41 1.9282978231445876E-4 0.0 0.0 0.14761119836171815 0.0 42-43 1.9282978231445876E-4 0.0 0.0 0.1953278044944415 0.0 44-45 2.0159477241966142E-4 0.0 0.0 0.2537114035851965 0.0 46-47 2.1035976252486407E-4 0.0 0.0 0.3253608152001757 0.0 48-49 2.1035976252486407E-4 0.0 0.0 0.4064282086831952 0.0 50-51 2.1035976252486407E-4 0.0 0.0 0.4986052271245591 0.0 52-53 2.1912475263006676E-4 0.0 0.0 0.589055542515198 0.0 54-55 2.2788974273526942E-4 4.382495052601335E-6 0.0 0.6870130719309431 0.0 56-57 2.2788974273526942E-4 8.76499010520267E-6 0.0 0.8031053658743526 0.0 58-59 2.2788974273526942E-4 8.76499010520267E-6 0.0 0.9388969750792049 0.0 60-61 2.3227223778787078E-4 8.76499010520267E-6 0.0 1.089926519581952 0.0 62-63 2.366547328404721E-4 8.76499010520267E-6 0.0 1.2475955440893902 0.0 64-65 2.366547328404721E-4 8.76499010520267E-6 0.0 1.4038928476453645 4.382495052601335E-6 66-67 2.366547328404721E-4 8.76499010520267E-6 0.0 1.5826153783854995 8.76499010520267E-6 68-69 2.541847130508774E-4 8.76499010520267E-6 0.0 1.7934835103364652 8.76499010520267E-6 70-71 2.541847130508774E-4 8.76499010520267E-6 0.0 2.034279701001646 8.76499010520267E-6 72-73 2.541847130508774E-4 1.752998021040534E-5 0.0 2.2826400781276157 8.76499010520267E-6 74-75 2.541847130508774E-4 1.752998021040534E-5 0.0 2.524838667209629 1.3147485157804005E-5 76-77 2.541847130508774E-4 1.752998021040534E-5 0.0 2.7844752041059424 1.752998021040534E-5 78-79 2.629497031560801E-4 1.752998021040534E-5 0.0 3.0870952524781696 1.752998021040534E-5 80-81 2.629497031560801E-4 1.752998021040534E-5 0.0 3.4288904241256004 1.752998021040534E-5 82-83 2.629497031560801E-4 1.752998021040534E-5 0.0 3.7888729502413265 1.752998021040534E-5 84-85 2.6733219820868146E-4 1.752998021040534E-5 0.0 4.137982506131548 1.752998021040534E-5 86-87 2.717146932612828E-4 1.752998021040534E-5 0.0 4.490444670737011 1.752998021040534E-5 88 2.717146932612828E-4 1.752998021040534E-5 0.0 4.777340326860505 1.752998021040534E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21665 0.0 54.050644 1 GTATCAA 41185 0.0 39.771748 1 ATCAACG 51075 0.0 31.300623 3 TCAACGC 51825 0.0 30.910847 4 CAACGCA 52115 0.0 30.702501 5 AACGCAG 53990 0.0 29.749393 6 TATCAAC 54150 0.0 29.710573 2 GTACATG 57065 0.0 29.437565 1 TACATGG 58055 0.0 28.465485 2 ACATGGG 56260 0.0 28.056747 3 ACGCAGA 61845 0.0 25.803505 7 CGCAGAG 62110 0.0 25.625319 8 CATGGGG 20680 0.0 24.541237 4 CATGGGA 31115 0.0 23.439325 4 GCAGAGT 69160 0.0 22.645933 9 AGAGTAC 65525 0.0 20.933449 10-11 GAGTACT 40000 0.0 20.59403 12-13 ACGGGCG 915 0.0 20.542738 5 TACCTGG 7570 0.0 20.190353 2 AGTACTT 43330 0.0 20.084993 12-13 >>END_MODULE