FastQCFastQC Report
Mon 6 Jun 2016
ERR1211548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12040603
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451560.3750310511857255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369160.306595940419263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256170.21275512530394033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209400.053.204621
GTATCAA391450.038.697781
ATCAACG462150.031.6025753
TCAACGC467500.031.2601174
CAACGCA468850.031.1875615
GTACATG516550.030.4470621
AACGCAG486700.030.1331446
TATCAAC500800.029.326042
ACATGGG510750.029.055493
TACATGG536100.028.7459322
ACGCAGA547850.026.6685057
CGCAGAG549000.026.476948
CATGGGG190050.025.3188484
CATGGGA290100.023.0984924
GCAGAGT616850.023.0105539
GAGTACT379050.020.83751912-13
CAGAGTA614550.020.0838510-11
AGAGTAC589000.019.94200910-11
ATGGGGG114550.019.3197525
TACCTGG78700.018.9421942
Produced by FastQC (version 0.11.5)