FastQCFastQC Report
Mon 6 Jun 2016
ERR1211548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12040603
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509270.42296054441791664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421380.3499658613443197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320810.26644014423530116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224500.051.4058881
GTATCAA407350.037.9131661
ATCAACG502300.030.1527543
TCAACGC509650.029.8005144
CAACGCA512700.029.6414415
AACGCAG531450.028.665936
GTACATG523000.028.657281
TATCAAC541700.028.0928152
ACATGGG517100.027.3358863
TACATGG547200.026.9596372
ACGCAGA602000.025.2047047
CGCAGAG602900.025.1359028
CATGGGG189050.023.3903854
CATGGGA296450.022.4934144
GCAGAGT673800.022.2119489
TACCTGG77100.021.6530762
AGAGTAC650100.020.4849610-11
GAGTACT411250.020.07668912-13
AGTACTT458200.019.22969612-13
CAGAGTA673850.019.11442610-11
Produced by FastQC (version 0.11.5)