FastQCFastQC Report
Mon 6 Jun 2016
ERR1211547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7628800
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260310.34122011325503354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235600.30882969798657717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136300.1786650587248322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133250.050.2198071
GTATCAA230950.037.722631
ATCAACG273150.031.0370033
TCAACGC272600.030.9785424
CAACGCA275450.030.672065
AACGCAG286750.029.603086
TATCAAC291800.029.263462
ACGCAGA317650.026.4437837
CGCAGAG324500.025.7997238
GTACATG243950.024.7477321
TACATGG246150.023.4963512
ACATGGG244200.022.8043213
GCAGAGT362300.022.3574499
GAGTACT225700.021.14067512-13
CAGAGTA349450.020.37598410-11
CATGGGG123100.019.81294
AGAGTAC333250.019.6323710-11
GTACTTT251050.018.92386214-15
TACCTGG53850.018.24972
AGTACTT248400.018.15892212-13
Produced by FastQC (version 0.11.5)