FastQCFastQC Report
Mon 6 Jun 2016
ERR1211547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7628800
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276540.3624947567114094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257160.33709102348993286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163220.2139523909395973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140800.050.0304831
GTATCAA239700.036.548571
TCAACGC292500.029.3182414
ATCAACG293150.029.1891173
CAACGCA297250.028.786135
AACGCAG308500.027.7967726
TATCAAC312550.027.5325052
ACGCAGA352700.024.2200537
CGCAGAG357100.023.895318
GTACATG251900.022.1402241
TACATGG256750.021.3734462
GCAGAGT398450.021.038029
ACATGGG253150.020.9007263
GAGTACT250950.020.55926112-13
CATGGGG127300.019.5635834
AGAGTAC371900.019.29930710-11
CAGAGTA392750.018.97464610-11
AGTACTT266950.018.94857212-13
GTACTTT271600.018.82465714-15
GTACCTG58900.018.7619551
Produced by FastQC (version 0.11.5)