FastQCFastQC Report
Mon 6 Jun 2016
ERR1211546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4888435
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168820.34534569857224245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156040.31920236231022814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96040.19646369441344724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83600.050.5220831
GTATCAA142300.036.266091
TACCTGG44050.033.73462
GTACCTG48450.032.07121
TCAACGC169750.029.7026634
ATCAACG170200.029.6796533
CAACGCA172250.029.1609525
TATCAAC180700.028.1060642
AACGCAG179600.028.0670416
ACCTGGG53550.026.8517023
GTACATG146050.025.9853521
ACGCAGA201050.025.0037527
CGCAGAG205700.024.2560528
TACATGG149750.024.1487062
ACATGGG145250.023.7136123
CATGGGG90250.022.7530524
TATAACG4150.021.5297952
GCAGAGT228900.021.1215659
GAGTACT151350.020.55253612-13
CAGAGTA223200.019.44033210-11
Produced by FastQC (version 0.11.5)